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The map label for this gene is nadB [C]

Identifier: 73538416

GI number: 73538416

Start: 1270991

End: 1272640

Strand: Reverse

Name: nadB [C]

Synonym: Reut_B4589

Alternate gene names: 73538416

Gene position: 1272640-1270991 (Counterclockwise)

Preceding gene: 73538417

Following gene: 73538415

Centisome position: 46.68

GC content: 67.39

Gene sequence:

>1650_bases
ATGCCAGCCGGCATCCCTTACGAAGACGCGCGCCAGCGCTATCGTCCGGGCGCAGAGCACGCGGTGCGCAGCGACGACAT
GCCCGTCGACGCATTGCTCAAGGCCTATCATCCGGACCATGGCGCGAACGCGCATGTCCCGCTGGCCGTTGGCGTCAACC
GCGGGGAGTCGTGCCAGCCTGACCTCGCGCGCTATCTGCAGGCCAATGCGCTGATCGACGATGTCGACATCGCCGGCGCG
CAACTGGCGACCACCGACGTGCTCGTCATCGGCGGCGGGGGCGGCGGGTGTGCCGCAGCGCTGACTGCGGCAAAGCAGGG
GGCGCGCGTGATTCTCGCCACCAAGTTGCGCCTTGGTGACAGCAACACCGTCATGGCCGAAGGCGGCATCCAGGCCGCCA
TCGGCGAGGACGACAGTCCTCAGCTGCATTTCGAAGACACGCTGCGGGCCGGGCATTTCTGCGGCGAGCCCGAACTTGTC
GCTCAGATGGTGATGGACGGGCCCGACGTGATTCGCTGGCTGATCCGGCTCGGCATGATGTTCGACCAGGAAGAGGACCG
GCTTATCGGCAGCAATCTGCTGCGCAAGAAGCCAGGCGGTGCGTCGGCCGCGCGCATTCTTTCGTATCGCGACTACACCG
GGCTCGAGATGATGCGCGTGCTGCGCGAGGCGGTCGACCTCGATTCCGGCATCGACGTGTGGAACCGTTGCCCTGCGGTC
GAACTGCTGTCCGACGAGCGCGGGCGCTGCGCTGGCGCGGTGATCTACAACCTGGAGTTGCGCACCTTCGTGCTGGTCCG
CGCGCGGTCCGTGATCCTGGCCACGGGCGGCGCGGGGCGACTGCATCTGAATGCGTTCCCGACTTCGAATCACTATGGTG
CGACTGCGGACGGTCTTGTCCTTGCCTATCGTCTCGGCGCGCGCCTGCGCGAACTCGATTCGTTCCAGTACCACCCGACC
GGCATCGCGTATCCGTCCCACCTGGCGGGTGGGCTGATTTCGGAAGCAGCGCGTTCCGCGGGCGCAAAACTGCTCAATGG
CAACGGCGAACGCTTCGTGGAGGAACTCAAGCCTCGCGATGTCGTGGCCTCGGCCATCTTGCGCGAATGCGCCGAGGGGC
GAGGCATCGACCGCGATGGCCAGGTCGGCGTGTTCCTCGACACGCCGACGCTGGAAATGCAGAACCCGGGCATCCTTTCC
AGGCGGCTCGTGACGCTGCGGCATCTCGCGCACAAGTGTGGGTTCGATGCGGCACAGGAGCCCTTCATGGTCTACCCAAC
CCTCCACTACCAGAACGGGGGCGTGGCGATCGGGAAGGACGGCGCGACGAGCGTGCCTGGCCTTTATTGCGTGGGCGAAG
TCACGGGCGGCATTCACGGACGCAACCGCCTGATGGGCAATGCGCTGCTCGATATCCTCAGCATGGGCCGGCATGCCGGC
GCCAGCGCCGCATCAGCGGGCGGCAACGCCATGGCCAACCGCGCCGGCATCGGCCACGTGCATGCCTGGCAGCGGGAACT
GACGCTCGCCGGGCTGCCGCTGCACGTCCAGGCACCGCTGCTGTTCCCCGGCTACGCGCATTTCGATCTGCGCGTCGACG
CGGCCCGCAGCCCGGGCGCGTGGGGCCGGGCGCCCGGTGGCATCCGTTAG

Upstream 100 bases:

>100_bases
CAATCTCATGCGGCGCCTGCAGCAGATCGAGCGCGGCGAAATGGGCATCGATTTCGATGCGCCGGGCGCCCGCCCGCCGC
GCTGACGGGGGCGAACCGTC

Downstream 100 bases:

>100_bases
CGATAGGGCGATAGCGATCGGCGGAGCGCGTCATGGAAAAGCTGAATCAGGTCTTGCTTAAGGAGGACATCCGGGTCGGC
GCAGACCTGCAGGCCTGGCT

Product: L-aspartate oxidase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MPAGIPYEDARQRYRPGAEHAVRSDDMPVDALLKAYHPDHGANAHVPLAVGVNRGESCQPDLARYLQANALIDDVDIAGA
QLATTDVLVIGGGGGGCAAALTAAKQGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGEPELV
AQMVMDGPDVIRWLIRLGMMFDQEEDRLIGSNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDSGIDVWNRCPAV
ELLSDERGRCAGAVIYNLELRTFVLVRARSVILATGGAGRLHLNAFPTSNHYGATADGLVLAYRLGARLRELDSFQYHPT
GIAYPSHLAGGLISEAARSAGAKLLNGNGERFVEELKPRDVVASAILRECAEGRGIDRDGQVGVFLDTPTLEMQNPGILS
RRLVTLRHLAHKCGFDAAQEPFMVYPTLHYQNGGVAIGKDGATSVPGLYCVGEVTGGIHGRNRLMGNALLDILSMGRHAG
ASAASAGGNAMANRAGIGHVHAWQRELTLAGLPLHVQAPLLFPGYAHFDLRVDAARSPGAWGRAPGGIR

Sequences:

>Translated_549_residues
MPAGIPYEDARQRYRPGAEHAVRSDDMPVDALLKAYHPDHGANAHVPLAVGVNRGESCQPDLARYLQANALIDDVDIAGA
QLATTDVLVIGGGGGGCAAALTAAKQGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGEPELV
AQMVMDGPDVIRWLIRLGMMFDQEEDRLIGSNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDSGIDVWNRCPAV
ELLSDERGRCAGAVIYNLELRTFVLVRARSVILATGGAGRLHLNAFPTSNHYGATADGLVLAYRLGARLRELDSFQYHPT
GIAYPSHLAGGLISEAARSAGAKLLNGNGERFVEELKPRDVVASAILRECAEGRGIDRDGQVGVFLDTPTLEMQNPGILS
RRLVTLRHLAHKCGFDAAQEPFMVYPTLHYQNGGVAIGKDGATSVPGLYCVGEVTGGIHGRNRLMGNALLDILSMGRHAG
ASAASAGGNAMANRAGIGHVHAWQRELTLAGLPLHVQAPLLFPGYAHFDLRVDAARSPGAWGRAPGGIR
>Mature_548_residues
PAGIPYEDARQRYRPGAEHAVRSDDMPVDALLKAYHPDHGANAHVPLAVGVNRGESCQPDLARYLQANALIDDVDIAGAQ
LATTDVLVIGGGGGGCAAALTAAKQGARVILATKLRLGDSNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGEPELVA
QMVMDGPDVIRWLIRLGMMFDQEEDRLIGSNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDSGIDVWNRCPAVE
LLSDERGRCAGAVIYNLELRTFVLVRARSVILATGGAGRLHLNAFPTSNHYGATADGLVLAYRLGARLRELDSFQYHPTG
IAYPSHLAGGLISEAARSAGAKLLNGNGERFVEELKPRDVVASAILRECAEGRGIDRDGQVGVFLDTPTLEMQNPGILSR
RLVTLRHLAHKCGFDAAQEPFMVYPTLHYQNGGVAIGKDGATSVPGLYCVGEVTGGIHGRNRLMGNALLDILSMGRHAGA
SAASAGGNAMANRAGIGHVHAWQRELTLAGLPLHVQAPLLFPGYAHFDLRVDAARSPGAWGRAPGGIR

Specific function: Catalyzes The Oxidation Of L-Aspartate To Iminoaspartate. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=407, Percent_Identity=32.9238329238329, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1788928, Length=414, Percent_Identity=34.5410628019324, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1786942, Length=425, Percent_Identity=35.0588235294118, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790597, Length=412, Percent_Identity=32.5242718446602, Blast_Score=155, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17550100, Length=402, Percent_Identity=32.3383084577114, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=399, Percent_Identity=31.8295739348371, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=478, Percent_Identity=28.2426778242678, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322701, Length=428, Percent_Identity=32.7102803738318, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322416, Length=420, Percent_Identity=33.0952380952381, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320788, Length=483, Percent_Identity=28.9855072463768, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322511, Length=475, Percent_Identity=26.1052631578947, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137288, Length=477, Percent_Identity=32.9140461215933, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655642, Length=477, Percent_Identity=32.9140461215933, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24655647, Length=477, Percent_Identity=32.9140461215933, Blast_Score=177, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24663005, Length=426, Percent_Identity=31.2206572769953, Blast_Score=142, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 58417; Mature: 58286

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAGIPYEDARQRYRPGAEHAVRSDDMPVDALLKAYHPDHGANAHVPLAVGVNRGESCQP
CCCCCCHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
DLARYLQANALIDDVDIAGAQLATTDVLVIGGGGGGCAAALTAAKQGARVILATKLRLGD
HHHHHHHHCCCEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGEPELVAQMVMDGPDVIRWLIRLGMM
CCEEEECCCEEEECCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
FDQEEDRLIGSNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDSGIDVWNRCPAV
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCH
ELLSDERGRCAGAVIYNLELRTFVLVRARSVILATGGAGRLHLNAFPTSNHYGATADGLV
HHHCCCCCCEEEEEEEEEEHHHEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LAYRLGARLRELDSFQYHPTGIAYPSHLAGGLISEAARSAGAKLLNGNGERFVEELKPRD
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHH
VVASAILRECAEGRGIDRDGQVGVFLDTPTLEMQNPGILSRRLVTLRHLAHKCGFDAAQE
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
PFMVYPTLHYQNGGVAIGKDGATSVPGLYCVGEVTGGIHGRNRLMGNALLDILSMGRHAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
ASAASAGGNAMANRAGIGHVHAWQRELTLAGLPLHVQAPLLFPGYAHFDLRVDAARSPGA
CCCCCCCCCHHHCCCCCCCHHHHHHHHEEECCCEEEECCEECCCCEEEEEEEECCCCCCC
WGRAPGGIR
CCCCCCCCC
>Mature Secondary Structure 
PAGIPYEDARQRYRPGAEHAVRSDDMPVDALLKAYHPDHGANAHVPLAVGVNRGESCQP
CCCCCHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
DLARYLQANALIDDVDIAGAQLATTDVLVIGGGGGGCAAALTAAKQGARVILATKLRLGD
HHHHHHHHCCCEECCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECC
SNTVMAEGGIQAAIGEDDSPQLHFEDTLRAGHFCGEPELVAQMVMDGPDVIRWLIRLGMM
CCEEEECCCEEEECCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
FDQEEDRLIGSNLLRKKPGGASAARILSYRDYTGLEMMRVLREAVDLDSGIDVWNRCPAV
CCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCH
ELLSDERGRCAGAVIYNLELRTFVLVRARSVILATGGAGRLHLNAFPTSNHYGATADGLV
HHHCCCCCCEEEEEEEEEEHHHEEEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCHHH
LAYRLGARLRELDSFQYHPTGIAYPSHLAGGLISEAARSAGAKLLNGNGERFVEELKPRD
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHH
VVASAILRECAEGRGIDRDGQVGVFLDTPTLEMQNPGILSRRLVTLRHLAHKCGFDAAQE
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCC
PFMVYPTLHYQNGGVAIGKDGATSVPGLYCVGEVTGGIHGRNRLMGNALLDILSMGRHAG
CEEEEEEEEECCCCEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
ASAASAGGNAMANRAGIGHVHAWQRELTLAGLPLHVQAPLLFPGYAHFDLRVDAARSPGA
CCCCCCCCCHHHCCCCCCCHHHHHHHHEEECCCEEEECCEECCCCEEEEEEEECCCCCCC
WGRAPGGIR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]