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The map label for this gene is nuoF2 [H]

Identifier: 73538415

GI number: 73538415

Start: 1269714

End: 1270958

Strand: Reverse

Name: nuoF2 [H]

Synonym: Reut_B4588

Alternate gene names: 73538415

Gene position: 1270958-1269714 (Counterclockwise)

Preceding gene: 73538416

Following gene: 73538414

Centisome position: 46.62

GC content: 65.38

Gene sequence:

>1245_bases
ATGGAAAAGCTGAATCAGGTCTTGCTTAAGGAGGACATCCGGGTCGGCGCAGACCTGCAGGCCTGGCTTGCGCGTGGTGG
CGGAGAAGGGCTGGCCAAGGCTTTGTCCAATCCGGATGCGATCCTTGCGGAGATCGAACAGGCCGATCTGCGCGGCATGG
GCGGCGCGGGGTTCCCGACGCACCGCAAGTGGAGGCCAGTTGCTGCTGCAGCCGCGGTCGACAAGTACATCATCTGCAAT
GGCAACGAAGACGAACCCGGCACGTTCAAGGACCGCTTCCTTCTGGAGCACACGCCGCACCAGGTCATCGAGGGCGCGCT
GATCGCGGCGGCAGCCACGCGGGCCAATCATGTCGTGCTCTACGTCAATCCGCATCAGCCACTGTCGCTCGAAATGACGC
GGCAGGCGGTGCAGCAGTGGCAAACACATGCGCAACTCGCCGAACTCGAGCGGCTGCTCGGCGGGCCGCTGACGCTGCGG
GTCGTGCCGAGTTCCGGCCTGTACATCGGTGGCGAGGAAACGGCCGTGATAGCCAGCGTCGAGGGTACGTTCCCATTTCC
GCGACGCAAGCCGCCTTATCCCGCCGACAGCGGCGTGCACGGCGCGCCGACCATCGTCAACAACACCGAGACGCTCGCCC
ACGTGCCGGGGATACTTCGCCACGGCGCGCAGTGGTATCGCGCGCTCGGCGTCGCTCAGGCGGCAGGGACCAAGCTCTAT
TCGCTGTCGGGCGACGTCCTGCGCCCGGGGCTGTACGAGCTGCCGATGGGCACCAGCCTGGAGACACTGATCTTCGAGTT
TGGCGGCGGCATGCTGCAGGGCAAGGAATTCAAGGCTGTCTTCACGGGCGGGCCGTCCAACACGCTGCTGACCAAGCGCG
ATCTCGACGTAGCGCTGGATTTCGACTCGGTGCGCAAGCGGCGCTCGCGCCTGGGGACCGGCGCGATGATCGTCGTATCG
GAGGGCTCCAGCATCGTCCGCAAGGTGGCCGAGTATGTGAGCTTCTTTGCGCAAGGTTCCTGCGGCCAGTGCCCGCCGTG
CAAGGGGGGCACATTCCAGCTGATGCGATTGCTCAACCGGATCGACACGGGCCGCGGCGTGCGCGCCGACCTGGAGGCGC
TGGAGAACCTGTGCCGCATCCTTCCCGGCAGCGGTCGCTGCGGGCTGATCGACGGCGCTGTCACGGTGGTAGACAGTTCG
CTTCACCAGTTCCGCGATGAATATGAGTCGCAGCTGATGGCATAG

Upstream 100 bases:

>100_bases
TCGATCTGCGCGTCGACGCGGCCCGCAGCCCGGGCGCGTGGGGCCGGGCGCCCGGTGGCATCCGTTAGCGATAGGGCGAT
AGCGATCGGCGGAGCGCGTC

Downstream 100 bases:

>100_bases
ACGGCAGCGGCAATCAGCGGCCCGGCCGTTCACGGGACGGTCGGTCCGCACGCAGAGGAGTCCACCATGTCCTACAGGGC
ATCGTATGCGTTGTGCCTGG

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEKLNQVLLKEDIRVGADLQAWLARGGGEGLAKALSNPDAILAEIEQADLRGMGGAGFPTHRKWRPVAAAAAVDKYIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHVVLYVNPHQPLSLEMTRQAVQQWQTHAQLAELERLLGGPLTLR
VVPSSGLYIGGEETAVIASVEGTFPFPRRKPPYPADSGVHGAPTIVNNTETLAHVPGILRHGAQWYRALGVAQAAGTKLY
SLSGDVLRPGLYELPMGTSLETLIFEFGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRKRRSRLGTGAMIVVS
EGSSIVRKVAEYVSFFAQGSCGQCPPCKGGTFQLMRLLNRIDTGRGVRADLEALENLCRILPGSGRCGLIDGAVTVVDSS
LHQFRDEYESQLMA

Sequences:

>Translated_414_residues
MEKLNQVLLKEDIRVGADLQAWLARGGGEGLAKALSNPDAILAEIEQADLRGMGGAGFPTHRKWRPVAAAAAVDKYIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHVVLYVNPHQPLSLEMTRQAVQQWQTHAQLAELERLLGGPLTLR
VVPSSGLYIGGEETAVIASVEGTFPFPRRKPPYPADSGVHGAPTIVNNTETLAHVPGILRHGAQWYRALGVAQAAGTKLY
SLSGDVLRPGLYELPMGTSLETLIFEFGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRKRRSRLGTGAMIVVS
EGSSIVRKVAEYVSFFAQGSCGQCPPCKGGTFQLMRLLNRIDTGRGVRADLEALENLCRILPGSGRCGLIDGAVTVVDSS
LHQFRDEYESQLMA
>Mature_414_residues
MEKLNQVLLKEDIRVGADLQAWLARGGGEGLAKALSNPDAILAEIEQADLRGMGGAGFPTHRKWRPVAAAAAVDKYIICN
GNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHVVLYVNPHQPLSLEMTRQAVQQWQTHAQLAELERLLGGPLTLR
VVPSSGLYIGGEETAVIASVEGTFPFPRRKPPYPADSGVHGAPTIVNNTETLAHVPGILRHGAQWYRALGVAQAAGTKLY
SLSGDVLRPGLYELPMGTSLETLIFEFGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRKRRSRLGTGAMIVVS
EGSSIVRKVAEYVSFFAQGSCGQCPPCKGGTFQLMRLLNRIDTGRGVRADLEALENLCRILPGSGRCGLIDGAVTVVDSS
LHQFRDEYESQLMA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=381, Percent_Identity=36.745406824147, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI260656005, Length=381, Percent_Identity=36.745406824147, Blast_Score=239, Evalue=4e-63,
Organism=Escherichia coli, GI1788620, Length=396, Percent_Identity=39.8989898989899, Blast_Score=284, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17531727, Length=384, Percent_Identity=35.9375, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI19920794, Length=381, Percent_Identity=35.4330708661417, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45550452, Length=398, Percent_Identity=34.6733668341709, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24653819, Length=389, Percent_Identity=32.6478149100257, Blast_Score=184, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=32.3943661971831, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 44619; Mature: 44619

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLNQVLLKEDIRVGADLQAWLARGGGEGLAKALSNPDAILAEIEQADLRGMGGAGFPT
CCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
HRKWRPVAAAAAVDKYIICNGNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHVVL
CCCCCCHHHHHHHCCEEEECCCCCCCCCHHHHEHHCCCHHHHHHHHHHHHHHHCCCEEEE
YVNPHQPLSLEMTRQAVQQWQTHAQLAELERLLGGPLTLRVVPSSGLYIGGEETAVIASV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCEEEEEEE
EGTFPFPRRKPPYPADSGVHGAPTIVNNTETLAHVPGILRHGAQWYRALGVAQAAGTKLY
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SLSGDVLRPGLYELPMGTSLETLIFEFGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALD
ECCCCCCCCCCEECCCCCCHHHHHHHHCCCEECCCEEEEEEECCCCCCEEEECCCEEEEC
FDSVRKRRSRLGTGAMIVVSEGSSIVRKVAEYVSFFAQGSCGQCPPCKGGTFQLMRLLNR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDTGRGVRADLEALENLCRILPGSGRCGLIDGAVTVVDSSLHQFRDEYESQLMA
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKLNQVLLKEDIRVGADLQAWLARGGGEGLAKALSNPDAILAEIEQADLRGMGGAGFPT
CCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
HRKWRPVAAAAAVDKYIICNGNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHVVL
CCCCCCHHHHHHHCCEEEECCCCCCCCCHHHHEHHCCCHHHHHHHHHHHHHHHCCCEEEE
YVNPHQPLSLEMTRQAVQQWQTHAQLAELERLLGGPLTLRVVPSSGLYIGGEETAVIASV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCEEEEEEE
EGTFPFPRRKPPYPADSGVHGAPTIVNNTETLAHVPGILRHGAQWYRALGVAQAAGTKLY
CCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
SLSGDVLRPGLYELPMGTSLETLIFEFGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALD
ECCCCCCCCCCEECCCCCCHHHHHHHHCCCEECCCEEEEEEECCCCCCEEEECCCEEEEC
FDSVRKRRSRLGTGAMIVVSEGSSIVRKVAEYVSFFAQGSCGQCPPCKGGTFQLMRLLNR
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
IDTGRGVRADLEALENLCRILPGSGRCGLIDGAVTVVDSSLHQFRDEYESQLMA
HCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]