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The map label for this gene is gldA [H]

Identifier: 73538397

GI number: 73538397

Start: 1250369

End: 1251466

Strand: Reverse

Name: gldA [H]

Synonym: Reut_B4570

Alternate gene names: 73538397

Gene position: 1251466-1250369 (Counterclockwise)

Preceding gene: 73538398

Following gene: 73538396

Centisome position: 45.91

GC content: 66.03

Gene sequence:

>1098_bases
ATGGTGCGTACGATGGGTTTTCCGGGGCAATATCTGCAAGGGCCTGGCGCCCTGCGGCAACTGCCCCCGCTACTGCAGCG
CATGGGCTGGCGGCGCCCGTTGGCGATCTGCGACGCCATCGTTGCGGAGAAGGTCTGGCCCGCCGTGGCGCAGGCACTGT
GCGAAGCCGGAGTCGCGGCAAGGCAACTGGCCTTTCCCGGGGAATGCACGCGGGCGGTGATCGCCGGGATGGTGGATCAG
GCGTCGCAGGGCAGCCATGACGTGGTCATTGGCCTGGGCGGCGGCAAGGCACTCGACACCGCCAAGGGCGTGGCGCGCGC
GCTTGACGTTCCCATTGTCATTTCTCCGACCATTGCGTCGAACGATGCGCCGACGAGCCGCGTGATTGTCCTGTACGACG
ATGCACACCGGCTGGAAGGCGCCGAATTCCTGAAGACCAATCCCGCAGCGGTGATCGTCGACACGCAGGTCATCGCGCAG
GCGCCGGTGCGCTTCTTCGCCGCCGGCATCGGCGACGCCATCAGCAAGAAATTCGAGGCTGCGCAATGCAGGGCGACGGG
ACGCAACAACGCATTCGGAACGCCGCCGCTGGATACGGCATTGCTGCTCGCTGGCGCTACCTATGAAACACTGGTCAAAC
ACGGACCTGCCGCCTGCGAAGCTATCCGGCGCCAGGAGCCCAATGAAGCCGTGGAACGTGTGGTCGAGGCGACCGTGCTG
CTCAGTGGCCTGGGCTTCGAAGGCGGAGGACTGGCGCTTGCCCATGCGCTGACGCGCGGCTTCAGCGCGGTCCCCGGCAT
GGCGCAGGCGCTGCATGGCGAACAGGTCGCCTTTGGTGCGCTGGTCCAACTGGTGGCCGAGCAGCGAGACCAGGAGGAGA
TCGCAGAACTTGTCGATCTGCTCTGCGCGGTCGGCCTGCCTGTCACGCTGCAACAGCTAGGGGTGGCGCCCGAGAACCAT
CACGACAGCATTGCCACCATCGTCCAGGCCACGTTGGCGGGACAGACGAGCAACGTCATCAGCCCTTCTCTCAACCCTCA
GCAACTGGCTGCGGCGCTCGCGCATGCCGACAGTATCGGCACGCTGGCCCTCGGCTGA

Upstream 100 bases:

>100_bases
GCAATCCGCGAGCAGGACAACGGCTTGCGGTACATCGCCGCCGCGGTCCCGACGTACGCGGCCTGACCGCAGCACGCCAA
TCTTGACGGAGGCGATCTGT

Downstream 100 bases:

>100_bases
CCGGCGGGAAATTCCTTCTCTGCAGGAGCAACTATCCATGATGCACACATTGAAGCGGCTGGTGGCCGTTACGGCCACCG
TCGCCGCCATGAGTCCGTTC

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH; GlyDH [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MVRTMGFPGQYLQGPGALRQLPPLLQRMGWRRPLAICDAIVAEKVWPAVAQALCEAGVAARQLAFPGECTRAVIAGMVDQ
ASQGSHDVVIGLGGGKALDTAKGVARALDVPIVISPTIASNDAPTSRVIVLYDDAHRLEGAEFLKTNPAAVIVDTQVIAQ
APVRFFAAGIGDAISKKFEAAQCRATGRNNAFGTPPLDTALLLAGATYETLVKHGPAACEAIRRQEPNEAVERVVEATVL
LSGLGFEGGGLALAHALTRGFSAVPGMAQALHGEQVAFGALVQLVAEQRDQEEIAELVDLLCAVGLPVTLQQLGVAPENH
HDSIATIVQATLAGQTSNVISPSLNPQQLAAALAHADSIGTLALG

Sequences:

>Translated_365_residues
MVRTMGFPGQYLQGPGALRQLPPLLQRMGWRRPLAICDAIVAEKVWPAVAQALCEAGVAARQLAFPGECTRAVIAGMVDQ
ASQGSHDVVIGLGGGKALDTAKGVARALDVPIVISPTIASNDAPTSRVIVLYDDAHRLEGAEFLKTNPAAVIVDTQVIAQ
APVRFFAAGIGDAISKKFEAAQCRATGRNNAFGTPPLDTALLLAGATYETLVKHGPAACEAIRRQEPNEAVERVVEATVL
LSGLGFEGGGLALAHALTRGFSAVPGMAQALHGEQVAFGALVQLVAEQRDQEEIAELVDLLCAVGLPVTLQQLGVAPENH
HDSIATIVQATLAGQTSNVISPSLNPQQLAAALAHADSIGTLALG
>Mature_365_residues
MVRTMGFPGQYLQGPGALRQLPPLLQRMGWRRPLAICDAIVAEKVWPAVAQALCEAGVAARQLAFPGECTRAVIAGMVDQ
ASQGSHDVVIGLGGGKALDTAKGVARALDVPIVISPTIASNDAPTSRVIVLYDDAHRLEGAEFLKTNPAAVIVDTQVIAQ
APVRFFAAGIGDAISKKFEAAQCRATGRNNAFGTPPLDTALLLAGATYETLVKHGPAACEAIRRQEPNEAVERVVEATVL
LSGLGFEGGGLALAHALTRGFSAVPGMAQALHGEQVAFGALVQLVAEQRDQEEIAELVDLLCAVGLPVTLQQLGVAPENH
HDSIATIVQATLAGQTSNVISPSLNPQQLAAALAHADSIGTLALG

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=361, Percent_Identity=39.612188365651, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786815, Length=373, Percent_Identity=27.3458445040214, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 37803; Mature: 37803

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTMGFPGQYLQGPGALRQLPPLLQRMGWRRPLAICDAIVAEKVWPAVAQALCEAGVAA
CCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RQLAFPGECTRAVIAGMVDQASQGSHDVVIGLGGGKALDTAKGVARALDVPIVISPTIAS
HHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCC
NDAPTSRVIVLYDDAHRLEGAEFLKTNPAAVIVDTQVIAQAPVRFFAAGIGDAISKKFEA
CCCCCCEEEEEECCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AQCRATGRNNAFGTPPLDTALLLAGATYETLVKHGPAACEAIRRQEPNEAVERVVEATVL
HHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
LSGLGFEGGGLALAHALTRGFSAVPGMAQALHGEQVAFGALVQLVAEQRDQEEIAELVDL
HHCCCCCCCHHHHHHHHHHCHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LCAVGLPVTLQQLGVAPENHHDSIATIVQATLAGQTSNVISPSLNPQQLAAALAHADSIG
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TLALG
CCCCC
>Mature Secondary Structure
MVRTMGFPGQYLQGPGALRQLPPLLQRMGWRRPLAICDAIVAEKVWPAVAQALCEAGVAA
CCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RQLAFPGECTRAVIAGMVDQASQGSHDVVIGLGGGKALDTAKGVARALDVPIVISPTIAS
HHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCC
NDAPTSRVIVLYDDAHRLEGAEFLKTNPAAVIVDTQVIAQAPVRFFAAGIGDAISKKFEA
CCCCCCEEEEEECCCCCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AQCRATGRNNAFGTPPLDTALLLAGATYETLVKHGPAACEAIRRQEPNEAVERVVEATVL
HHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHH
LSGLGFEGGGLALAHALTRGFSAVPGMAQALHGEQVAFGALVQLVAEQRDQEEIAELVDL
HHCCCCCCCHHHHHHHHHHCHHCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHH
LCAVGLPVTLQQLGVAPENHHDSIATIVQATLAGQTSNVISPSLNPQQLAAALAHADSIG
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCC
TLALG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]