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The map label for this gene is ydgK [H]

Identifier: 73538275

GI number: 73538275

Start: 1109156

End: 1110430

Strand: Reverse

Name: ydgK [H]

Synonym: Reut_B4448

Alternate gene names: 73538275

Gene position: 1110430-1109156 (Counterclockwise)

Preceding gene: 73538276

Following gene: 73538272

Centisome position: 40.73

GC content: 64.86

Gene sequence:

>1275_bases
ATGGAAATGAAGGGCAGCAGAGCTGATGAACGGGCAGCACCCGGTGTGCTGACCGCGCAGAGCGATCCACAGCCGAAGGC
CCTTCCTGGCATTGCCGCCATGGCGGCCATTGCAGCGGGATTGTCGATGCTCGGGCAGTTTGCCATCGCGACCTATCTGC
CCGCCTTCGCGATCATGAGCGATGCGCTGCACGCGACGAACGCGCAGGTCCAGCAGTCGCTCACTGCCTATCTGCTGCCC
TTCGCGCTGATGCTGCCGTGGCACGGCGCCATCTCCGATGCCGTGGGCCGCCGGCGGATGGTTTTGCTCGGTTGTGCGTT
GTTCACCATCGGCTCACTGGTCTGTGCCGGGGCCACTGGCATCGATATGCTTCACGCCGGGCGGGTACTGCAGGGCATCA
GTGCCGGCATTGGCGTGATCGTCGGCCGGGCCATGGTGCGCGATGTCTTCGACGGCATGCAGGCCCAGCAGGTCATGGCG
CTGGTCGCGATGATCTTTGCGCTGGCTCCGGCGATCGCGCCGGTCTGCGGCGGCTGGCTCTTGCTGTGGACAGGCTGGCG
CAGCATCTTTGTCTTCCTGGCGCTCGTGTCGGCGTCGCTGCTCGTCGCCTGCTGGCGATGGGTGCCCGAAACCTTGCCGC
CCGCGCGCCGCCATCCGCTTCATCCCGCTTTGCTGGCGCGCGGCTATGGGTCCATGTTGACCCATTGGCGCTTTGTCGCG
CTGACGCTCGCGAACGCCGGGGTCAACGTCGGCATCTATGTCTATATCTTTGCCGCGCCCACGTTCGTGACCCGGCATCT
CGGGCTTGGTGCGCAGTCGTTCGGCTGGCTATTCATCCCGATCGTAGCGGGGATCATGCTGGGCTCGCTCGTGTCGCATC
GCATGGCCGGCAGGATCAGCCCGGTGCGCAGCGTGCTGCTCGGGCACGCTGTCATGCTGTTCGCCGGCATATTGAACATT
GTGCTCACGGCGCTGCACGCCCCGGCCATGCCGTGGGCGCTGATCGCACTGCCCGTCTACGGCGCCGGCATGACCATCAC
GCAACCGAGCCTGCAATTGCTGGCGCTCGATTGTTTCCCGGAACGGCGCGGGCTCGCATCGAGTGGCTATGTCACGGTGC
AGCAGTTGGGCAATTTCCTGATGTCAGCGCTGCTGGTCCCGCTGCTGCTGGATTCGACAGTCAAGCTGGCGGTTTGTATG
GCAGCGCTGCAGTTATTCGGCCTCGCCATGTTCATGGGCGGGCAACGCGACAGGGCAGTCGTGGTTTCTTCGTGA

Upstream 100 bases:

>100_bases
ATGAATTGCCATTCTTTTGGCGCGATTTGTGGCTTTTGAAATGCTTCGCGCCAATACCATCGGGTCCATACATTGCGCTG
CCTGGCAAAGGGAGACCCGA

Downstream 100 bases:

>100_bases
AACCAACGTTCAACCCGCCAACGCCACATCAATGGCGCGCTGCAGCCGTTCCATACCGAAGGTCACGTCCTGCACCACGC
CATTGACGAAGAACGATGGC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MEMKGSRADERAAPGVLTAQSDPQPKALPGIAAMAAIAAGLSMLGQFAIATYLPAFAIMSDALHATNAQVQQSLTAYLLP
FALMLPWHGAISDAVGRRRMVLLGCALFTIGSLVCAGATGIDMLHAGRVLQGISAGIGVIVGRAMVRDVFDGMQAQQVMA
LVAMIFALAPAIAPVCGGWLLLWTGWRSIFVFLALVSASLLVACWRWVPETLPPARRHPLHPALLARGYGSMLTHWRFVA
LTLANAGVNVGIYVYIFAAPTFVTRHLGLGAQSFGWLFIPIVAGIMLGSLVSHRMAGRISPVRSVLLGHAVMLFAGILNI
VLTALHAPAMPWALIALPVYGAGMTITQPSLQLLALDCFPERRGLASSGYVTVQQLGNFLMSALLVPLLLDSTVKLAVCM
AALQLFGLAMFMGGQRDRAVVVSS

Sequences:

>Translated_424_residues
MEMKGSRADERAAPGVLTAQSDPQPKALPGIAAMAAIAAGLSMLGQFAIATYLPAFAIMSDALHATNAQVQQSLTAYLLP
FALMLPWHGAISDAVGRRRMVLLGCALFTIGSLVCAGATGIDMLHAGRVLQGISAGIGVIVGRAMVRDVFDGMQAQQVMA
LVAMIFALAPAIAPVCGGWLLLWTGWRSIFVFLALVSASLLVACWRWVPETLPPARRHPLHPALLARGYGSMLTHWRFVA
LTLANAGVNVGIYVYIFAAPTFVTRHLGLGAQSFGWLFIPIVAGIMLGSLVSHRMAGRISPVRSVLLGHAVMLFAGILNI
VLTALHAPAMPWALIALPVYGAGMTITQPSLQLLALDCFPERRGLASSGYVTVQQLGNFLMSALLVPLLLDSTVKLAVCM
AALQLFGLAMFMGGQRDRAVVVSS
>Mature_424_residues
MEMKGSRADERAAPGVLTAQSDPQPKALPGIAAMAAIAAGLSMLGQFAIATYLPAFAIMSDALHATNAQVQQSLTAYLLP
FALMLPWHGAISDAVGRRRMVLLGCALFTIGSLVCAGATGIDMLHAGRVLQGISAGIGVIVGRAMVRDVFDGMQAQQVMA
LVAMIFALAPAIAPVCGGWLLLWTGWRSIFVFLALVSASLLVACWRWVPETLPPARRHPLHPALLARGYGSMLTHWRFVA
LTLANAGVNVGIYVYIFAAPTFVTRHLGLGAQSFGWLFIPIVAGIMLGSLVSHRMAGRISPVRSVLLGHAVMLFAGILNI
VLTALHAPAMPWALIALPVYGAGMTITQPSLQLLALDCFPERRGLASSGYVTVQQLGNFLMSALLVPLLLDSTVKLAVCM
AALQLFGLAMFMGGQRDRAVVVSS

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=329, Percent_Identity=27.355623100304, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082312, Length=348, Percent_Identity=28.735632183908, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790146, Length=332, Percent_Identity=25.6024096385542, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787065, Length=339, Percent_Identity=26.2536873156342, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI48994889, Length=250, Percent_Identity=26, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790195, Length=205, Percent_Identity=29.2682926829268, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790794, Length=281, Percent_Identity=20.2846975088968, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44830; Mature: 44830

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMKGSRADERAAPGVLTAQSDPQPKALPGIAAMAAIAAGLSMLGQFAIATYLPAFAIMS
CCCCCCCCCHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALHATNAQVQQSLTAYLLPFALMLPWHGAISDAVGRRRMVLLGCALFTIGSLVCAGATG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDMLHAGRVLQGISAGIGVIVGRAMVRDVFDGMQAQQVMALVAMIFALAPAIAPVCGGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLWTGWRSIFVFLALVSASLLVACWRWVPETLPPARRHPLHPALLARGYGSMLTHWRFVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHH
LTLANAGVNVGIYVYIFAAPTFVTRHLGLGAQSFGWLFIPIVAGIMLGSLVSHRMAGRIS
HHHHCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
PVRSVLLGHAVMLFAGILNIVLTALHAPAMPWALIALPVYGAGMTITQPSLQLLALDCFP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHEEECCH
ERRGLASSGYVTVQQLGNFLMSALLVPLLLDSTVKLAVCMAALQLFGLAMFMGGQRDRAV
HHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VVSS
EECC
>Mature Secondary Structure
MEMKGSRADERAAPGVLTAQSDPQPKALPGIAAMAAIAAGLSMLGQFAIATYLPAFAIMS
CCCCCCCCCHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALHATNAQVQQSLTAYLLPFALMLPWHGAISDAVGRRRMVLLGCALFTIGSLVCAGATG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDMLHAGRVLQGISAGIGVIVGRAMVRDVFDGMQAQQVMALVAMIFALAPAIAPVCGGWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LLWTGWRSIFVFLALVSASLLVACWRWVPETLPPARRHPLHPALLARGYGSMLTHWRFVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHH
LTLANAGVNVGIYVYIFAAPTFVTRHLGLGAQSFGWLFIPIVAGIMLGSLVSHRMAGRIS
HHHHCCCCCEEEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
PVRSVLLGHAVMLFAGILNIVLTALHAPAMPWALIALPVYGAGMTITQPSLQLLALDCFP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEECCCHHHHEEECCH
ERRGLASSGYVTVQQLGNFLMSALLVPLLLDSTVKLAVCMAALQLFGLAMFMGGQRDRAV
HHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VVSS
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]