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The map label for this gene is garD [H]

Identifier: 73538108

GI number: 73538108

Start: 934081

End: 935604

Strand: Reverse

Name: garD [H]

Synonym: Reut_B4278

Alternate gene names: 73538108

Gene position: 935604-934081 (Counterclockwise)

Preceding gene: 73538109

Following gene: 73538107

Centisome position: 34.32

GC content: 64.5

Gene sequence:

>1524_bases
GTGAAAACGAACCCCGTCATCCGGCTGCATGCTAACGATAACGTGCTGATCGCCAGGGGCGATCTTGCGCTCGGGCAGCA
GCTGTCCGATCCCGCGTTGCGCGTGCGCGCCCAAGTCCCGGCCGGACACAAGATCGCGGCCAGCGCGATTGCCAGCGGCA
CGCCGGTGCGCAAGTACGACACGGTGATCGGCGTGGCGTCGCGCGATATCGTAGCTGGCGAGCACGTGCATTCGCACAAT
CTGAAGCTGGTGGATTTCTACCGTGACCCGGCCTTCTGCCAGGACGTTCGCGCGGTGGAATACGTGCCGGAAGCCGAGCG
GGCCACGTTCATGGGTTTCGTTCGCCCGGACGGCCGCGTGGGCACGCGCAATTTCATCGGCATCCTGTCGTCGGTGAACT
GTTCGTCGACAGTGATTCGCCAGATTGCCTCGCATTTCACGCCGGAGCGGCTGGCCGCCTATCCGAACGTGGATGGCGTA
GTCGCCTTCGCCCAGACCAGCGGTTGCGGCATGTCGTCGCCGAGCGAGCATTTCGACGTGCTGCGCCGGACGCTGGCCGG
GTATGCGCGGCATCCCAACCTGGCGGGTGTGCTGATCGTTGGACTCGGCTGCGAGCGCAATCAGGTCGCAGGTCTGGTGG
AATCGCAAGGGCTCGAACCCGGCGCGGCCATGCACACGCTGGTCATGCAGGACAGCGGCGGCACGCGTGAAACGATCCAG
GCAGGTATTCGCGCCGTTGAATCGATGTTGCCTGCTGCGAACCAGGCCGTGCGCCAACGCGTGCCAGCCAGCCACCTGAA
GATCGGCCTCGAGTGCGGCGGTTCGGACGGGTTCTCGGGCATCAGTGCGAATCCAGCGCTCGGTGCGGCCATGGACATCC
TGGTACGGCACGGGGGCACTGCGATCCTGTCGGAAACGCCGGAGATCCACGGTGTGGAGTTCATGCTGACGCGCCGCGCG
GTGACGCCGGAAGTCGGCCAGAAGCTGCTGGACCGCCTGGCCTGGTGGGAGCGCTATACCGCCGGCCACAACGCGCAGTT
CAACGGCGTGGTAGGCCACGGCAACCAGCAAGGCGGCCTGGCGAACATCTTTGAAAAGTCGCTGGGATCGGCCATGAAGG
GCGGCACCACGCCGCTGCAGGCGGTGTACGAGTATGCTGAGCCCATCGACAAGGCCGGCTTCGTCTTCATGGATTCTCCC
GGCTACGACCCGGTCGCCGTGACGGGCCAGATTGCCAGCGGCGCCAACCTGATCTGCTTCACCACCGGCCGTGGTTCCAT
GTTCGGCTCCAAGCCGGCGCCCACCATCAAGCTCGCGTCGAACACGCCGATGTACGAACGTTTGCGCGACGACATGGACA
TCAATTGCGGTCTGGTGGTCGATGGCGAGCTGACGATCGGGCAGATGGGGCAGCGCATTTTCGAACATATTCTGCAAGCG
GCATCTGGCCAACCAACTCGAAGCGAACTGCTGGGCCTTGGCGACAACGAGTTCGTGCCCTGGCATCTCGGTATCGTGAG
CTGA

Upstream 100 bases:

>100_bases
CCCGAATACAAGAAGTACGTGGCGACGGTCTATGACCAGGAAACCCAGCTGATCCAGAAGCTGCGACTCAAGGAACTGCT
GCAGCAAGGTTGATCCTCAA

Downstream 100 bases:

>100_bases
CCGCGTTCCCGCCTTCATCGCCCTCTTTTTGTACGTCCCATGTCCCTGACGCTTGCTAACGCCGCGCTGCTGCGCAGCCA
GAACTTCATCAATCAACAAT

Product: Altronate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKTNPVIRLHANDNVLIARGDLALGQQLSDPALRVRAQVPAGHKIAASAIASGTPVRKYDTVIGVASRDIVAGEHVHSHN
LKLVDFYRDPAFCQDVRAVEYVPEAERATFMGFVRPDGRVGTRNFIGILSSVNCSSTVIRQIASHFTPERLAAYPNVDGV
VAFAQTSGCGMSSPSEHFDVLRRTLAGYARHPNLAGVLIVGLGCERNQVAGLVESQGLEPGAAMHTLVMQDSGGTRETIQ
AGIRAVESMLPAANQAVRQRVPASHLKIGLECGGSDGFSGISANPALGAAMDILVRHGGTAILSETPEIHGVEFMLTRRA
VTPEVGQKLLDRLAWWERYTAGHNAQFNGVVGHGNQQGGLANIFEKSLGSAMKGGTTPLQAVYEYAEPIDKAGFVFMDSP
GYDPVAVTGQIASGANLICFTTGRGSMFGSKPAPTIKLASNTPMYERLRDDMDINCGLVVDGELTIGQMGQRIFEHILQA
ASGQPTRSELLGLGDNEFVPWHLGIVS

Sequences:

>Translated_507_residues
MKTNPVIRLHANDNVLIARGDLALGQQLSDPALRVRAQVPAGHKIAASAIASGTPVRKYDTVIGVASRDIVAGEHVHSHN
LKLVDFYRDPAFCQDVRAVEYVPEAERATFMGFVRPDGRVGTRNFIGILSSVNCSSTVIRQIASHFTPERLAAYPNVDGV
VAFAQTSGCGMSSPSEHFDVLRRTLAGYARHPNLAGVLIVGLGCERNQVAGLVESQGLEPGAAMHTLVMQDSGGTRETIQ
AGIRAVESMLPAANQAVRQRVPASHLKIGLECGGSDGFSGISANPALGAAMDILVRHGGTAILSETPEIHGVEFMLTRRA
VTPEVGQKLLDRLAWWERYTAGHNAQFNGVVGHGNQQGGLANIFEKSLGSAMKGGTTPLQAVYEYAEPIDKAGFVFMDSP
GYDPVAVTGQIASGANLICFTTGRGSMFGSKPAPTIKLASNTPMYERLRDDMDINCGLVVDGELTIGQMGQRIFEHILQA
ASGQPTRSELLGLGDNEFVPWHLGIVS
>Mature_507_residues
MKTNPVIRLHANDNVLIARGDLALGQQLSDPALRVRAQVPAGHKIAASAIASGTPVRKYDTVIGVASRDIVAGEHVHSHN
LKLVDFYRDPAFCQDVRAVEYVPEAERATFMGFVRPDGRVGTRNFIGILSSVNCSSTVIRQIASHFTPERLAAYPNVDGV
VAFAQTSGCGMSSPSEHFDVLRRTLAGYARHPNLAGVLIVGLGCERNQVAGLVESQGLEPGAAMHTLVMQDSGGTRETIQ
AGIRAVESMLPAANQAVRQRVPASHLKIGLECGGSDGFSGISANPALGAAMDILVRHGGTAILSETPEIHGVEFMLTRRA
VTPEVGQKLLDRLAWWERYTAGHNAQFNGVVGHGNQQGGLANIFEKSLGSAMKGGTTPLQAVYEYAEPIDKAGFVFMDSP
GYDPVAVTGQIASGANLICFTTGRGSMFGSKPAPTIKLASNTPMYERLRDDMDINCGLVVDGELTIGQMGQRIFEHILQA
ASGQPTRSELLGLGDNEFVPWHLGIVS

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=497, Percent_Identity=36.0160965794769, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1789475, Length=489, Percent_Identity=34.560327198364, Blast_Score=265, Evalue=7e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 53982; Mature: 53982

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNPVIRLHANDNVLIARGDLALGQQLSDPALRVRAQVPAGHKIAASAIASGTPVRKYD
CCCCCEEEEECCCCEEEEECCHHHCCCCCCCHHEEEEECCCCCHHHHHHHHCCCCCHHHH
TVIGVASRDIVAGEHVHSHNLKLVDFYRDPAFCQDVRAVEYVPEAERATFMGFVRPDGRV
HEEEECCCCEECCCCEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
GTRNFIGILSSVNCSSTVIRQIASHFTPERLAAYPNVDGVVAFAQTSGCGMSSPSEHFDV
CCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
LRRTLAGYARHPNLAGVLIVGLGCERNQVAGLVESQGLEPGAAMHTLVMQDSGGTRETIQ
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHEEEEEEECCCCCHHHHH
AGIRAVESMLPAANQAVRQRVPASHLKIGLECGGSDGFSGISANPALGAAMDILVRHGGT
HHHHHHHHHCCHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCE
AILSETPEIHGVEFMLTRRAVTPEVGQKLLDRLAWWERYTAGHNAQFNGVVGHGNQQGGL
EEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEECCCCCCCH
ANIFEKSLGSAMKGGTTPLQAVYEYAEPIDKAGFVFMDSPGYDPVAVTGQIASGANLICF
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEECCCCCEEEE
TTGRGSMFGSKPAPTIKLASNTPMYERLRDDMDINCGLVVDGELTIGQMGQRIFEHILQA
ECCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
ASGQPTRSELLGLGDNEFVPWHLGIVS
HCCCCCHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKTNPVIRLHANDNVLIARGDLALGQQLSDPALRVRAQVPAGHKIAASAIASGTPVRKYD
CCCCCEEEEECCCCEEEEECCHHHCCCCCCCHHEEEEECCCCCHHHHHHHHCCCCCHHHH
TVIGVASRDIVAGEHVHSHNLKLVDFYRDPAFCQDVRAVEYVPEAERATFMGFVRPDGRV
HEEEECCCCEECCCCEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
GTRNFIGILSSVNCSSTVIRQIASHFTPERLAAYPNVDGVVAFAQTSGCGMSSPSEHFDV
CCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
LRRTLAGYARHPNLAGVLIVGLGCERNQVAGLVESQGLEPGAAMHTLVMQDSGGTRETIQ
HHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHEEEEEEECCCCCHHHHH
AGIRAVESMLPAANQAVRQRVPASHLKIGLECGGSDGFSGISANPALGAAMDILVRHGGT
HHHHHHHHHCCHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCE
AILSETPEIHGVEFMLTRRAVTPEVGQKLLDRLAWWERYTAGHNAQFNGVVGHGNQQGGL
EEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEECCCCCCCH
ANIFEKSLGSAMKGGTTPLQAVYEYAEPIDKAGFVFMDSPGYDPVAVTGQIASGANLICF
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEECCCCCEEEE
TTGRGSMFGSKPAPTIKLASNTPMYERLRDDMDINCGLVVDGELTIGQMGQRIFEHILQA
ECCCCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
ASGQPTRSELLGLGDNEFVPWHLGIVS
HCCCCCHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2407727; 9772162; 10762278 [H]