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The map label for this gene is hpcH [H]

Identifier: 73538106

GI number: 73538106

Start: 931719

End: 932525

Strand: Reverse

Name: hpcH [H]

Synonym: Reut_B4276

Alternate gene names: 73538106

Gene position: 932525-931719 (Counterclockwise)

Preceding gene: 73538107

Following gene: 73538105

Centisome position: 34.21

GC content: 68.53

Gene sequence:

>807_bases
ATGCCCGCACACAATCCGTTCAAGGCCGCCCTGGCGGCCCGCCAGGCGCAGATCGGCCTCTGGCTCTCGATGGCGACGCC
TTACCTGGCCGAGGTGTCCGCCACCACCGGCTTCGACTGGCTCCTGATCGACGGCGAACACGCGCCGAACGACCTGCGTA
CGACGTTGCAGGCGCTGCAGGCTGTTGCGCCTTACCCGGCGCAGCCCGTGGTGCGTGCGCTGGCGGGCGAGGTAGCGCTG
ATCAAGCAGCTGCTCGATATCGGGGCGAAGAACCTGCTCGTGCCGATGGTCGATACGGCCGAGCAGGCGGCATCGCTGGT
CAGCGCCACACGCTACCCGCCGCAGGGCATTCGCGGCGTCGGCAGTGCGGTAGGACGTGCGTCGCGATGGAGCAGCCGTA
CGGACTACCTCGATGTGGCCGATGACGAGATCTGCCTGCTGGTGCAGGCGGAAACCGTGACGGCGCTGAAGAACCTGGAG
GCGATCTGCGCGGTGGAAGGTGTCGACGGCGTGTTCATCGGACCGGCAGACCTGGCCGCGTCGATGGGCTATCGCGGCAA
GCCGGGTCACCCCGAGGTGCAGGCGGCCATCGAAGGCGCGATGCGGACCATCGTCGCCAGCGGCAAGGCCGCTGGCACGC
TGACCTCGGACACGACGCTCGCGCGCCGCTACCTGGAGCTGGGCTGCACGTTCGTAGCGACCGGTGTGGACGTGACAATG
TACGCCAACGCTGCGCGACGGCTTGCCGCGGAGTTCAAGCCGCAACAGACGGCCAGCGCTGGTAGCGGGCCTTCCGCCGC
GTACTGA

Upstream 100 bases:

>100_bases
CGTCACGGGCTGGACGACTACATGCATACCAAGTACCTTTGCCAGGGCCAGCTCGGCTGAGCGGCAGTATTCCCGCATTC
ACAGAAGGCAAGACAACACC

Downstream 100 bases:

>100_bases
ACACCGAAACCGAATCCCGAGCGAACATGCAAGCCGAAGTCCAAGCCCCAAATCCCGAAACCACCCTGCGCGCGATGCAG
GCCATCGTCGGCGAGCACGC

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPAHNPFKAALAARQAQIGLWLSMATPYLAEVSATTGFDWLLIDGEHAPNDLRTTLQALQAVAPYPAQPVVRALAGEVAL
IKQLLDIGAKNLLVPMVDTAEQAASLVSATRYPPQGIRGVGSAVGRASRWSSRTDYLDVADDEICLLVQAETVTALKNLE
AICAVEGVDGVFIGPADLAASMGYRGKPGHPEVQAAIEGAMRTIVASGKAAGTLTSDTTLARRYLELGCTFVATGVDVTM
YANAARRLAAEFKPQQTASAGSGPSAAY

Sequences:

>Translated_268_residues
MPAHNPFKAALAARQAQIGLWLSMATPYLAEVSATTGFDWLLIDGEHAPNDLRTTLQALQAVAPYPAQPVVRALAGEVAL
IKQLLDIGAKNLLVPMVDTAEQAASLVSATRYPPQGIRGVGSAVGRASRWSSRTDYLDVADDEICLLVQAETVTALKNLE
AICAVEGVDGVFIGPADLAASMGYRGKPGHPEVQAAIEGAMRTIVASGKAAGTLTSDTTLARRYLELGCTFVATGVDVTM
YANAARRLAAEFKPQQTASAGSGPSAAY
>Mature_267_residues
PAHNPFKAALAARQAQIGLWLSMATPYLAEVSATTGFDWLLIDGEHAPNDLRTTLQALQAVAPYPAQPVVRALAGEVALI
KQLLDIGAKNLLVPMVDTAEQAASLVSATRYPPQGIRGVGSAVGRASRWSSRTDYLDVADDEICLLVQAETVTALKNLEA
ICAVEGVDGVFIGPADLAASMGYRGKPGHPEVQAAIEGAMRTIVASGKAAGTLTSDTTLARRYLELGCTFVATGVDVTMY
ANAARRLAAEFKPQQTASAGSGPSAAY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=260, Percent_Identity=56.1538461538462, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=47.7911646586345, Blast_Score=237, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27967; Mature: 27836

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAHNPFKAALAARQAQIGLWLSMATPYLAEVSATTGFDWLLIDGEHAPNDLRTTLQALQ
CCCCCHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
AVAPYPAQPVVRALAGEVALIKQLLDIGAKNLLVPMVDTAEQAASLVSATRYPPQGIRGV
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHH
GSAVGRASRWSSRTDYLDVADDEICLLVQAETVTALKNLEAICAVEGVDGVFIGPADLAA
HHHHHHHHHCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHH
SMGYRGKPGHPEVQAAIEGAMRTIVASGKAAGTLTSDTTLARRYLELGCTFVATGVDVTM
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCCEEEE
YANAARRLAAEFKPQQTASAGSGPSAAY
HHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PAHNPFKAALAARQAQIGLWLSMATPYLAEVSATTGFDWLLIDGEHAPNDLRTTLQALQ
CCCCHHHHHHHHHHHHHHEEEHHHCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
AVAPYPAQPVVRALAGEVALIKQLLDIGAKNLLVPMVDTAEQAASLVSATRYPPQGIRGV
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHH
GSAVGRASRWSSRTDYLDVADDEICLLVQAETVTALKNLEAICAVEGVDGVFIGPADLAA
HHHHHHHHHCCCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHEEECCCCEEEECHHHHHH
SMGYRGKPGHPEVQAAIEGAMRTIVASGKAAGTLTSDTTLARRYLELGCTFVATGVDVTM
HCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEECCCEEEE
YANAARRLAAEFKPQQTASAGSGPSAAY
HHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA