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The map label for this gene is yogA [H]

Identifier: 73538093

GI number: 73538093

Start: 921023

End: 922051

Strand: Reverse

Name: yogA [H]

Synonym: Reut_B4263

Alternate gene names: 73538093

Gene position: 922051-921023 (Counterclockwise)

Preceding gene: 73538094

Following gene: 73538092

Centisome position: 33.82

GC content: 64.82

Gene sequence:

>1029_bases
ATGGAGACATTGATGCGCGCCGCCATCATCGACGCTACAGGTGGCCCCGGGGTGCTCAGGATTGCCAACGTTCCGGTTCC
CGAACTTGCCGCAGGCGAAGTGCTGATCCGTGTGATCTGTGCGGGCGTCAATCCCGCTGACTGGAAATGCCGCGAAGGTT
ATCTCGGCAACTTCATGCACTACAGCTTTCCGTTCGTGATCGGCTTTGATGTCAGCGGTGTCGTGGCGGCCGTGGGGGAG
GGCGTAACGGGATGGATTCCCGGCCAACGCGTGTTTGCACAAACCGACGTTGGCGCCGGCAAATGGGGCTCCTACGCCGA
GTACGTTGCCGTCAGTCAGGATTCGGTAGTCGCGATTCCGGATGGCCTTGGTGATGCCGAAGCGGCGGCGGTCCCCACGC
CCGCGCTGGCGGCGTGGGCGGGGCTATTTGACGAAGGGGGACTGAAGCCAGGACAGACAGTGCTCATCCACGGCGGTGGT
GGCGCCGTTGGCACCTTTGCCATCCAGTTGGCTCGCGCTGCCGGCGCCAGCGTGGCTGCCACCTGCAGCGCAAGCCGCTG
CGATGAACTGAAGTCGCTTGGCTGCGATCTGCCGATCGATTACAGGTCCGATCTGACCGAACGCGCTGTCGAGAAATGGG
CGCCGGGCGGCGTCGATCTTGTCCTGGACGCCGTCGGATGCGGCACGCTGCCGAATGGACTCGATTTACTGAAGCCAGGC
GGCATCCTCGTGGCCATCATGACGCTGGTCAGCGGAGACAGCGGCCCGGATGCGGCTGAGGCCGCGCGCCGAGGCAAGCG
TTGCGCGCTGGCGTATAGCCGGATGCCGAGCGGTGCGCGGCTACGCGAAATTTCCGAGCTGCTCGCAACAGGAAAAATCC
GGCCGCCCCGCGTCGAATGCCTGCCGCTGGAAGATGCAGCAGCAGCACTGGGGCTGGTCCAGTCAGGGAAGGCGCCTGCG
AAGCTTGTGCTGCAAGTCAGCAGCCCGGCCGAAGCTGAAGTCCCGGCGCAGCACCGCCGCGTTGCATAG

Upstream 100 bases:

>100_bases
GGTCTTTGGGCTTGATGGGGCGGGCAAACTGCCGCTACCCGTTGTTCCGGCCTGGGCGCATCCGGGTCTGCTCCGTTCAA
CTCTGCGGGAGGGCTGAATC

Downstream 100 bases:

>100_bases
CCCCGTAGATAACGTCCATCGAAGACACAGGAGACACATGAACAAGGCAATACTGCTTCGCACCCTGGCCGGTGCACTGG
TGACGGCTGCGCAGCTAGGC

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
METLMRAAIIDATGGPGVLRIANVPVPELAAGEVLIRVICAGVNPADWKCREGYLGNFMHYSFPFVIGFDVSGVVAAVGE
GVTGWIPGQRVFAQTDVGAGKWGSYAEYVAVSQDSVVAIPDGLGDAEAAAVPTPALAAWAGLFDEGGLKPGQTVLIHGGG
GAVGTFAIQLARAAGASVAATCSASRCDELKSLGCDLPIDYRSDLTERAVEKWAPGGVDLVLDAVGCGTLPNGLDLLKPG
GILVAIMTLVSGDSGPDAAEAARRGKRCALAYSRMPSGARLREISELLATGKIRPPRVECLPLEDAAAALGLVQSGKAPA
KLVLQVSSPAEAEVPAQHRRVA

Sequences:

>Translated_342_residues
METLMRAAIIDATGGPGVLRIANVPVPELAAGEVLIRVICAGVNPADWKCREGYLGNFMHYSFPFVIGFDVSGVVAAVGE
GVTGWIPGQRVFAQTDVGAGKWGSYAEYVAVSQDSVVAIPDGLGDAEAAAVPTPALAAWAGLFDEGGLKPGQTVLIHGGG
GAVGTFAIQLARAAGASVAATCSASRCDELKSLGCDLPIDYRSDLTERAVEKWAPGGVDLVLDAVGCGTLPNGLDLLKPG
GILVAIMTLVSGDSGPDAAEAARRGKRCALAYSRMPSGARLREISELLATGKIRPPRVECLPLEDAAAALGLVQSGKAPA
KLVLQVSSPAEAEVPAQHRRVA
>Mature_342_residues
METLMRAAIIDATGGPGVLRIANVPVPELAAGEVLIRVICAGVNPADWKCREGYLGNFMHYSFPFVIGFDVSGVVAAVGE
GVTGWIPGQRVFAQTDVGAGKWGSYAEYVAVSQDSVVAIPDGLGDAEAAAVPTPALAAWAGLFDEGGLKPGQTVLIHGGG
GAVGTFAIQLARAAGASVAATCSASRCDELKSLGCDLPIDYRSDLTERAVEKWAPGGVDLVLDAVGCGTLPNGLDLLKPG
GILVAIMTLVSGDSGPDAAEAARRGKRCALAYSRMPSGARLREISELLATGKIRPPRVECLPLEDAAAALGLVQSGKAPA
KLVLQVSSPAEAEVPAQHRRVA

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=360, Percent_Identity=34.1666666666667, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI24308257, Length=253, Percent_Identity=33.9920948616601, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI18379349, Length=316, Percent_Identity=30.0632911392405, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI194239674, Length=252, Percent_Identity=30.1587301587302, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI13236495, Length=252, Percent_Identity=30.1587301587302, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI194239676, Length=185, Percent_Identity=35.1351351351351, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI22538446, Length=341, Percent_Identity=29.6187683284457, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI22538444, Length=341, Percent_Identity=29.6187683284457, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28557745, Length=245, Percent_Identity=31.0204081632653, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI90669511, Length=291, Percent_Identity=29.553264604811, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790485, Length=314, Percent_Identity=27.7070063694268, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507255, Length=245, Percent_Identity=32.6530612244898, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71987554, Length=373, Percent_Identity=27.3458445040214, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=350, Percent_Identity=26, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17562584, Length=352, Percent_Identity=24.4318181818182, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=338, Percent_Identity=27.2189349112426, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324195, Length=251, Percent_Identity=27.8884462151394, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581339, Length=256, Percent_Identity=30.46875, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24581341, Length=256, Percent_Identity=30.46875, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550423, Length=300, Percent_Identity=28.6666666666667, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34988; Mature: 34988

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLMRAAIIDATGGPGVLRIANVPVPELAAGEVLIRVICAGVNPADWKCREGYLGNFMH
CCHHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEE
YSFPFVIGFDVSGVVAAVGEGVTGWIPGQRVFAQTDVGAGKWGSYAEYVAVSQDSVVAIP
ECCCEEEECCHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCHHEEEECCCCEEEEC
DGLGDAEAAAVPTPALAAWAGLFDEGGLKPGQTVLIHGGGGAVGTFAIQLARAAGASVAA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
TCSASRCDELKSLGCDLPIDYRSDLTERAVEKWAPGGVDLVLDAVGCGTLPNGLDLLKPG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCC
GILVAIMTLVSGDSGPDAAEAARRGKRCALAYSRMPSGARLREISELLATGKIRPPRVEC
HHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCEEE
LPLEDAAAALGLVQSGKAPAKLVLQVSSPAEAEVPAQHRRVA
EECHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCC
>Mature Secondary Structure
METLMRAAIIDATGGPGVLRIANVPVPELAAGEVLIRVICAGVNPADWKCREGYLGNFMH
CCHHHHHHEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCEEE
YSFPFVIGFDVSGVVAAVGEGVTGWIPGQRVFAQTDVGAGKWGSYAEYVAVSQDSVVAIP
ECCCEEEECCHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCCCHHEEEECCCCEEEEC
DGLGDAEAAAVPTPALAAWAGLFDEGGLKPGQTVLIHGGGGAVGTFAIQLARAAGASVAA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEE
TCSASRCDELKSLGCDLPIDYRSDLTERAVEKWAPGGVDLVLDAVGCGTLPNGLDLLKPG
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCC
GILVAIMTLVSGDSGPDAAEAARRGKRCALAYSRMPSGARLREISELLATGKIRPPRVEC
HHHHHHHHHHCCCCCCCHHHHHHCCCEEEEHHHCCCCCHHHHHHHHHHHHCCCCCCCEEE
LPLEDAAAALGLVQSGKAPAKLVLQVSSPAEAEVPAQHRRVA
EECHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]