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The map label for this gene is mexA [H]

Identifier: 73537923

GI number: 73537923

Start: 733530

End: 734690

Strand: Reverse

Name: mexA [H]

Synonym: Reut_B4090

Alternate gene names: 73537923

Gene position: 734690-733530 (Counterclockwise)

Preceding gene: 73537929

Following gene: 73537922

Centisome position: 26.95

GC content: 69.34

Gene sequence:

>1161_bases
ATGCAACGATCACGCGGCTGCCGCGCCTGGGGTCCGGTTACGCTGGCCATCGCCCTGTCCACGCTCGCCGGATGCGGCGG
AAAAAAAGAAGCCGCCGCCCCGCCCCCGCCCCAGGTCAGCGTGGTCACGCTGAAGACCGAAGCAGTCACGCTGGATACCT
CGCTTCCCGGCCGCACCACGGCCTACCGCATTGCCGAAGTCCGGCCCCAGGTGGACGGCATTATCCTGCGGCGGCTGTTC
AAGGAAGGCAGCGACGTGACCCAGGGCCAGCAGCTCTATCAGATCGACCCGTCTACCTACGATGCGACCTACAAGAGCGC
CAGGGCAACGATGGAGTCATCGCGGCTGCTGGCGCAGCGCTACGGACGCCTGGTTGGCGACGAAGCGGTCAGCAAGCAGC
AGTATGACGAGGCCAAGGCCGCGTGGCAGCAGGCCCAGGCGACGCTGGACCGGGCCGAGATCAACCTGCGCTACACCAAG
GTGCTGGCGCCCATTTCGGGGCGGATCGGCCGTTCCAGCGTGACCGAGGGCGCGCTGGTTACCAACGGGCAGGCGGCGGC
GCTCGCCGTTGTGCAGCAGCTCGATCCGATCTACGTCGATGTGACGCAGCCTTCCGCATCGCTGCTCCGGCTGCGGCGCG
AACTCGCCAACGGCGCGTTGCAGAGCGCCGGCACCAATGCGGCGCAGGTCAACCTGGTGCTCGAAGACGGCACGAAATAC
CCCGAGACCGGCCGGCTGGAGTTCTCCGAGGTCGCGGTGGACCAGGGCACCGGCTCGGTCACGCTGCGCGCCGTGTTCCC
CAACCCGCGCCACGAGCTGCTGCCCGGCATGTTCGTCCACGCGCGCCTGCGCGAAGGCGTGAAGACCGACGGCCTGCTCG
TGCCCCAGCAAGGCGTTACGCGTGACCTCAAGGGCCAGGCCACGGCGCTGGTGGTCAACGCGAAGGACGAGGTCGAACTG
CGCCAGATCCGCACCGACCGCGCCATCGGCGACAAATGGCTGGTGGCCGACGGCCTGAAGGCCGGCGACCGTGTGATCGT
CGAAGGCGTGCAGTTCGTCAAGCCCGGCGCGAAGGTCCGCGTCGCCCAGGCAGACAGCGGCACAGGCAACCCGCCCGCGG
CCGCCGCCGGCGCATCCGCCGCGGTCGCGGCCAAGCCCTGA

Upstream 100 bases:

>100_bases
GGGCATGGCATTTGCTAAGCCTCCCTATCCGGCTCCGGACGGCTGTCGTGATTGACCGCCGAACACCTTGATTCGCTTGA
TTTTTTCGCAGGAACTCGCA

Downstream 100 bases:

>100_bases
GCGCGTTACGAGGGCGAGGGAGTATTCATGTCCAATTTCTTTATCGATCGCCCTATCTTCGCGTGGGTGATCGCGCTGGT
CATCATGCTGGCCGGCGTGC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQRSRGCRAWGPVTLAIALSTLAGCGGKKEAAAPPPPQVSVVTLKTEAVTLDTSLPGRTTAYRIAEVRPQVDGIILRRLF
KEGSDVTQGQQLYQIDPSTYDATYKSARATMESSRLLAQRYGRLVGDEAVSKQQYDEAKAAWQQAQATLDRAEINLRYTK
VLAPISGRIGRSSVTEGALVTNGQAAALAVVQQLDPIYVDVTQPSASLLRLRRELANGALQSAGTNAAQVNLVLEDGTKY
PETGRLEFSEVAVDQGTGSVTLRAVFPNPRHELLPGMFVHARLREGVKTDGLLVPQQGVTRDLKGQATALVVNAKDEVEL
RQIRTDRAIGDKWLVADGLKAGDRVIVEGVQFVKPGAKVRVAQADSGTGNPPAAAAGASAAVAAKP

Sequences:

>Translated_386_residues
MQRSRGCRAWGPVTLAIALSTLAGCGGKKEAAAPPPPQVSVVTLKTEAVTLDTSLPGRTTAYRIAEVRPQVDGIILRRLF
KEGSDVTQGQQLYQIDPSTYDATYKSARATMESSRLLAQRYGRLVGDEAVSKQQYDEAKAAWQQAQATLDRAEINLRYTK
VLAPISGRIGRSSVTEGALVTNGQAAALAVVQQLDPIYVDVTQPSASLLRLRRELANGALQSAGTNAAQVNLVLEDGTKY
PETGRLEFSEVAVDQGTGSVTLRAVFPNPRHELLPGMFVHARLREGVKTDGLLVPQQGVTRDLKGQATALVVNAKDEVEL
RQIRTDRAIGDKWLVADGLKAGDRVIVEGVQFVKPGAKVRVAQADSGTGNPPAAAAGASAAVAAKP
>Mature_386_residues
MQRSRGCRAWGPVTLAIALSTLAGCGGKKEAAAPPPPQVSVVTLKTEAVTLDTSLPGRTTAYRIAEVRPQVDGIILRRLF
KEGSDVTQGQQLYQIDPSTYDATYKSARATMESSRLLAQRYGRLVGDEAVSKQQYDEAKAAWQQAQATLDRAEINLRYTK
VLAPISGRIGRSSVTEGALVTNGQAAALAVVQQLDPIYVDVTQPSASLLRLRRELANGALQSAGTNAAQVNLVLEDGTKY
PETGRLEFSEVAVDQGTGSVTLRAVFPNPRHELLPGMFVHARLREGVKTDGLLVPQQGVTRDLKGQATALVVNAKDEVEL
RQIRTDRAIGDKWLVADGLKAGDRVIVEGVQFVKPGAKVRVAQADSGTGNPPAAAAGASAAVAAKP

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=396, Percent_Identity=54.7979797979798, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=54.1208791208791, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=47.2527472527472, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI87082036, Length=362, Percent_Identity=26.5193370165746, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41023; Mature: 41023

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRSRGCRAWGPVTLAIALSTLAGCGGKKEAAAPPPPQVSVVTLKTEAVTLDTSLPGRTT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCC
AYRIAEVRPQVDGIILRRLFKEGSDVTQGQQLYQIDPSTYDATYKSARATMESSRLLAQR
EEEEEHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
YGRLVGDEAVSKQQYDEAKAAWQQAQATLDRAEINLRYTKVLAPISGRIGRSSVTEGALV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCEE
TNGQAAALAVVQQLDPIYVDVTQPSASLLRLRRELANGALQSAGTNAAQVNLVLEDGTKY
ECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
PETGRLEFSEVAVDQGTGSVTLRAVFPNPRHELLPGMFVHARLREGVKTDGLLVPQQGVT
CCCCCEEHHHEEEECCCCCEEEEEECCCCHHHHCCCHHHHHHHHCCCCCCCEEECCCCCC
RDLKGQATALVVNAKDEVELRQIRTDRAIGDKWLVADGLKAGDRVIVEGVQFVKPGAKVR
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCHHHCCCCCEEE
VAQADSGTGNPPAAAAGASAAVAAKP
EEECCCCCCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MQRSRGCRAWGPVTLAIALSTLAGCGGKKEAAAPPPPQVSVVTLKTEAVTLDTSLPGRTT
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCC
AYRIAEVRPQVDGIILRRLFKEGSDVTQGQQLYQIDPSTYDATYKSARATMESSRLLAQR
EEEEEHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
YGRLVGDEAVSKQQYDEAKAAWQQAQATLDRAEINLRYTKVLAPISGRIGRSSVTEGALV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCCCEE
TNGQAAALAVVQQLDPIYVDVTQPSASLLRLRRELANGALQSAGTNAAQVNLVLEDGTKY
ECCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
PETGRLEFSEVAVDQGTGSVTLRAVFPNPRHELLPGMFVHARLREGVKTDGLLVPQQGVT
CCCCCEEHHHEEEECCCCCEEEEEECCCCHHHHCCCHHHHHHHHCCCCCCCEEECCCCCC
RDLKGQATALVVNAKDEVELRQIRTDRAIGDKWLVADGLKAGDRVIVEGVQFVKPGAKVR
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEECCHHHCCCCCEEE
VAQADSGTGNPPAAAAGASAAVAAKP
EEECCCCCCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]