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The map label for this gene is qor [C]

Identifier: 73537746

GI number: 73537746

Start: 544449

End: 545465

Strand: Direct

Name: qor [C]

Synonym: Reut_B3912

Alternate gene names: 73537746

Gene position: 544449-545465 (Clockwise)

Preceding gene: 73537745

Following gene: 73537749

Centisome position: 19.97

GC content: 66.08

Gene sequence:

>1017_bases
ATGAAAGCCATCGGCCTGACCCGCTACCTGCCCATTTCCGACCCCGATTCTCTGGTCGACATCGAACTTGAAACGCCTGC
TGCGTCGGGCCACGACCTGCTGGTCAAGGTCGAGGCCATCGCCGTCAATCCGGTCGACACCAAGGTCCGCGCACCGAAGC
CGCAGGTCGAGCCGAACCCGCGCGTGCTGGGCTGGGATGCAGCCGGCACGGTTGTCGCCGTGGGGCCGGATGTCAGCCTG
TTCAAGGTTGGCGATCCTGTCTACTACGCGGGCAGCATCACTCGTCCCGGCGCCAATGCGGAATACCACCTCGTGGATGA
GCGCATTGCCGGGCACAAGCCGGCCTCGCTCGATTTCGCGCACGCCGCCGCGTTGCCGCTGACGGCCATCACCGCGTGGG
AAGCGCTGTTCGACCGGCTCGGCGTGTCGCCCAAGGGCGACGATGCGGGCAAGTCGGTGCTGATCATTGGCGGTGCGGGC
GGCGTGGGGTCCATCGGCATCCAGCTTGCCAAGACGCTGGCGGGGCTGACGGTGATTGCCACGGCGGCGCGGCCAGAGTC
GCAGGCGTGGTGCCGTCGGCTGGGCGCGGACCATACCGTGGACCATCGCAGCGACATGGCTGCGCAATTGAAGGCGCTCG
GGTTTGCGCAGGTCGACTACATCCTGTGCTTCAACGATACCGATGGCCATTTCCCGGTCATGGCGGACCTGATCGCACCG
CAGGGCAAGATCTGCACGATCGTCGAGAACGCGGCGCCGCTGCCGGTGGAACGGCTCAAGAGCAAAAGCGCGGCGTTTGT
GTGGGAATTCATGTTCACGCGCGCGATGTTCGGCACGCCCGACATGATCGCGCAGCACCGGCTGCTCAACGAGGTGGCGC
GATTGGTGGATGCCGGGCGACTGCAGACCACGGTAGGCGAACATCTCGGCGCCATCAACGCGGCCAACCTGCGCCGCGCC
CATGCGATGCTGGAGGGCGGCAAGACCATCGGCAAGCTGGTGCTGGAAGGGTTCTGA

Upstream 100 bases:

>100_bases
CGCTAATCAAACAAGTCAATTGCGGCTACGCTACAGTCCATCTCCCTGAACCGGCTTGCGGGCACACCTGCGGGCCGGCA
CGAACCACCGGACTGTGACC

Downstream 100 bases:

>100_bases
CCCGGTCCGAGAGCGTACCCGAAAGACGAAAAGCGCCGGCCGCATATTGAGCGGTCAGGCGCTTTTCGCGTTGATTAGTG
CATCAACGCATCTTGCCGAG

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKAIGLTRYLPISDPDSLVDIELETPAASGHDLLVKVEAIAVNPVDTKVRAPKPQVEPNPRVLGWDAAGTVVAVGPDVSL
FKVGDPVYYAGSITRPGANAEYHLVDERIAGHKPASLDFAHAAALPLTAITAWEALFDRLGVSPKGDDAGKSVLIIGGAG
GVGSIGIQLAKTLAGLTVIATAARPESQAWCRRLGADHTVDHRSDMAAQLKALGFAQVDYILCFNDTDGHFPVMADLIAP
QGKICTIVENAAPLPVERLKSKSAAFVWEFMFTRAMFGTPDMIAQHRLLNEVARLVDAGRLQTTVGEHLGAINAANLRRA
HAMLEGGKTIGKLVLEGF

Sequences:

>Translated_338_residues
MKAIGLTRYLPISDPDSLVDIELETPAASGHDLLVKVEAIAVNPVDTKVRAPKPQVEPNPRVLGWDAAGTVVAVGPDVSL
FKVGDPVYYAGSITRPGANAEYHLVDERIAGHKPASLDFAHAAALPLTAITAWEALFDRLGVSPKGDDAGKSVLIIGGAG
GVGSIGIQLAKTLAGLTVIATAARPESQAWCRRLGADHTVDHRSDMAAQLKALGFAQVDYILCFNDTDGHFPVMADLIAP
QGKICTIVENAAPLPVERLKSKSAAFVWEFMFTRAMFGTPDMIAQHRLLNEVARLVDAGRLQTTVGEHLGAINAANLRRA
HAMLEGGKTIGKLVLEGF
>Mature_338_residues
MKAIGLTRYLPISDPDSLVDIELETPAASGHDLLVKVEAIAVNPVDTKVRAPKPQVEPNPRVLGWDAAGTVVAVGPDVSL
FKVGDPVYYAGSITRPGANAEYHLVDERIAGHKPASLDFAHAAALPLTAITAWEALFDRLGVSPKGDDAGKSVLIIGGAG
GVGSIGIQLAKTLAGLTVIATAARPESQAWCRRLGADHTVDHRSDMAAQLKALGFAQVDYILCFNDTDGHFPVMADLIAP
QGKICTIVENAAPLPVERLKSKSAAFVWEFMFTRAMFGTPDMIAQHRLLNEVARLVDAGRLQTTVGEHLGAINAANLRRA
HAMLEGGKTIGKLVLEGF

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=337, Percent_Identity=29.9703264094956, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI194239676, Length=217, Percent_Identity=33.6405529953917, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI194239674, Length=205, Percent_Identity=34.6341463414634, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI13236495, Length=205, Percent_Identity=34.6341463414634, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI18379349, Length=220, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI28557745, Length=360, Percent_Identity=28.0555555555556, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI24308257, Length=301, Percent_Identity=25.9136212624585, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI22538446, Length=355, Percent_Identity=26.1971830985915, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI22538444, Length=355, Percent_Identity=26.1971830985915, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790485, Length=203, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71987554, Length=342, Percent_Identity=28.6549707602339, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17507255, Length=240, Percent_Identity=31.6666666666667, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324195, Length=245, Percent_Identity=29.3877551020408, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319945, Length=237, Percent_Identity=30.379746835443, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323493, Length=244, Percent_Identity=29.5081967213115, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35800; Mature: 35800

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIGLTRYLPISDPDSLVDIELETPAASGHDLLVKVEAIAVNPVDTKVRAPKPQVEPNP
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCC
RVLGWDAAGTVVAVGPDVSLFKVGDPVYYAGSITRPGANAEYHLVDERIAGHKPASLDFA
CEEEECCCCEEEEECCCCEEEEECCCEEEECCCCCCCCCCEEEEEHHHHCCCCCCCCCHH
HAAALPLTAITAWEALFDRLGVSPKGDDAGKSVLIIGGAGGVGSIGIQLAKTLAGLTVIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEE
TAARPESQAWCRRLGADHTVDHRSDMAAQLKALGFAQVDYILCFNDTDGHFPVMADLIAP
ECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHCC
QGKICTIVENAAPLPVERLKSKSAAFVWEFMFTRAMFGTPDMIAQHRLLNEVARLVDAGR
CCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCH
LQTTVGEHLGAINAANLRRAHAMLEGGKTIGKLVLEGF
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MKAIGLTRYLPISDPDSLVDIELETPAASGHDLLVKVEAIAVNPVDTKVRAPKPQVEPNP
CCCCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCC
RVLGWDAAGTVVAVGPDVSLFKVGDPVYYAGSITRPGANAEYHLVDERIAGHKPASLDFA
CEEEECCCCEEEEECCCCEEEEECCCEEEECCCCCCCCCCEEEEEHHHHCCCCCCCCCHH
HAAALPLTAITAWEALFDRLGVSPKGDDAGKSVLIIGGAGGVGSIGIQLAKTLAGLTVIA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEE
TAARPESQAWCRRLGADHTVDHRSDMAAQLKALGFAQVDYILCFNDTDGHFPVMADLIAP
ECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHCC
QGKICTIVENAAPLPVERLKSKSAAFVWEFMFTRAMFGTPDMIAQHRLLNEVARLVDAGR
CCCEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCH
LQTTVGEHLGAINAANLRRAHAMLEGGKTIGKLVLEGF
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]