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The map label for this gene is lig [H]

Identifier: 73537729

GI number: 73537729

Start: 522871

End: 524526

Strand: Reverse

Name: lig [H]

Synonym: Reut_B3895

Alternate gene names: 73537729

Gene position: 524526-522871 (Counterclockwise)

Preceding gene: 73537730

Following gene: 73537724

Centisome position: 19.24

GC content: 68.3

Gene sequence:

>1656_bases
ATGAAGGCCTTTGCCGATCTCTACGCCGCGCTCGACGGCACCACCTCCACCAAGGCGCGGCTGGCCGCGCTGGTGGACTA
CCTGCGCGCGGCGCCGCCGCAGGACGCCGCCTGGGCCGTGTATTTCCTTGCAGGCGGCAAGCCACGCCAGATCGTGCCGG
TGTCCCTGCTGCGCGACATGGCACGCCAGGCCGCCGACCTGCCGGACTGGCTGTTCGAGGAAAGCTACCAGGCCGTGGGC
GACCTGGCCGAGACCATCGCGCTGTTGTTGCCGGACCCCATGGATTCTGATCACGCCGGCCTGGCCGAATGGGTCGAGCA
GCGCCTGCTGCCGCTACGCGGCCTGCCGCCTGACGAACTGATGCCGCGCCTGGATGCGCTGTGGCGGCCGCTCGACGCGC
ACGGCCGGCTGGTGCTGTTCAAGCTGATCACCGGCGCCTTTCGCGTGGGCGTATCGCGGCTGCTGGTGACGCGCGCGCTG
GGTGAAGTCGCCGGCATTGACCCCAAACGCGTGGCCGAGCGCATGGTCGGCTACACCGACCTGTCCGCGCGGCCCGACGC
AACGCGATTCCAGGCACTGCTAGCACCGGAAAGCGAGGACGACCGCGAACTGCGCGCCAACGGCCAGCCCTTCCCGTTCT
TCCTCGCGCATCCGCTGCAGGTTCCGCTGGAGCAGTTCGATGCCGTGCTGGGCGACCCGGACAACTGGCAGGTCGAATGG
AAGTGGGACGGCATCCGCGCGCAGCTAGTCTGCCGCGGCAACCAGACCTGGCTGTGGTCGCGCGGCGAGGAACTGATTAC
CGAACGCTTTCCCGAGATCGTCGAGGCCGCCGCGCCACTGCCTGACGGCACCGTACTCGACGGCGAGATCGTGATCTGGC
AACAGGGCCGCGTGCAGCCCTTCGCGCTGCTGCAGCAACGCATCGGCCGGAAGACGCTGAACGCGAAGCTGCTGCGCGAC
GCGCCGGCCATCCTGATGGCCTATGACCTGCTCGAATGGCGGGGAGAGGACTGGCGCACGCGGCCGCAAGCCGAGCGCCG
CACCCGGCTGGAACAGCTTGTCGCCAGTCACGTGCACCCGGCACTCGAACTGAGTCCGCTGGTGCAGGCCGGCGACTGGG
CGCACTTCGCCAGGCTGCGCGAGGCGTCGCGCGAGATGGGCGTGGAAGGCTTCATGTTGAAGGCCGCAAGCGCAGCCTAT
GGCGCGGGCCGCACCAAGGACGTCGGTATCTGGTGGAAATGGAAGATCGACCCATTCTCGGTCGATGCGGTGCTGGTCTA
CGCGCAGCGCGGCCACGGCCGCCGCGCGAGCCTCTATACCGACTTCACGTTCGCGGTCTGGAACGCGCCGCCAGGCACGC
CCAATCGCGCCCTGGTGCCGTTCGCCAAGGCCTATTCCGGCCTGACCGATGAGGAAATGCGCGCAGTCGATGCCATCGTT
CGGCGAACCACGGTGGAGAAGTTCGGCCCGGTGCGCAGCGTCGAGCCAACGCAGGTATTCGAGCTGGGCTTTGAAGGCAT
CGCCCGCAGCGGCCGGCACAAAAGCGGCATTGCGGTGCGCTTTCCGCGCATGTTGCGCTGGCGCACCGACAAGCCGATCG
ACGAGGCCGATACGCTAGCCACGCTGGAGGCGATGCTCCCCGTCAGTCCAGCGTAA

Upstream 100 bases:

>100_bases
GGCAGGCGCAGGCGTTCGATACCGAATACGGCGACGACGACGAATCCGCACGCGCCGATGAGCAGGCTGAGCCAGCCGCC
GCCAGTGAGGATACGGCGCC

Downstream 100 bases:

>100_bases
TCCCTTCCCGCTGGATCACCGCGCCCCAGGCCGCATCTTCCTTCTCGATGCGTTTGGCGAAGGTGGCCGAATCGATCATC
ACCACATCGCCCTGCTGCCC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKAFADLYAALDGTTSTKARLAALVDYLRAAPPQDAAWAVYFLAGGKPRQIVPVSLLRDMARQAADLPDWLFEESYQAVG
DLAETIALLLPDPMDSDHAGLAEWVEQRLLPLRGLPPDELMPRLDALWRPLDAHGRLVLFKLITGAFRVGVSRLLVTRAL
GEVAGIDPKRVAERMVGYTDLSARPDATRFQALLAPESEDDRELRANGQPFPFFLAHPLQVPLEQFDAVLGDPDNWQVEW
KWDGIRAQLVCRGNQTWLWSRGEELITERFPEIVEAAAPLPDGTVLDGEIVIWQQGRVQPFALLQQRIGRKTLNAKLLRD
APAILMAYDLLEWRGEDWRTRPQAERRTRLEQLVASHVHPALELSPLVQAGDWAHFARLREASREMGVEGFMLKAASAAY
GAGRTKDVGIWWKWKIDPFSVDAVLVYAQRGHGRRASLYTDFTFAVWNAPPGTPNRALVPFAKAYSGLTDEEMRAVDAIV
RRTTVEKFGPVRSVEPTQVFELGFEGIARSGRHKSGIAVRFPRMLRWRTDKPIDEADTLATLEAMLPVSPA

Sequences:

>Translated_551_residues
MKAFADLYAALDGTTSTKARLAALVDYLRAAPPQDAAWAVYFLAGGKPRQIVPVSLLRDMARQAADLPDWLFEESYQAVG
DLAETIALLLPDPMDSDHAGLAEWVEQRLLPLRGLPPDELMPRLDALWRPLDAHGRLVLFKLITGAFRVGVSRLLVTRAL
GEVAGIDPKRVAERMVGYTDLSARPDATRFQALLAPESEDDRELRANGQPFPFFLAHPLQVPLEQFDAVLGDPDNWQVEW
KWDGIRAQLVCRGNQTWLWSRGEELITERFPEIVEAAAPLPDGTVLDGEIVIWQQGRVQPFALLQQRIGRKTLNAKLLRD
APAILMAYDLLEWRGEDWRTRPQAERRTRLEQLVASHVHPALELSPLVQAGDWAHFARLREASREMGVEGFMLKAASAAY
GAGRTKDVGIWWKWKIDPFSVDAVLVYAQRGHGRRASLYTDFTFAVWNAPPGTPNRALVPFAKAYSGLTDEEMRAVDAIV
RRTTVEKFGPVRSVEPTQVFELGFEGIARSGRHKSGIAVRFPRMLRWRTDKPIDEADTLATLEAMLPVSPA
>Mature_551_residues
MKAFADLYAALDGTTSTKARLAALVDYLRAAPPQDAAWAVYFLAGGKPRQIVPVSLLRDMARQAADLPDWLFEESYQAVG
DLAETIALLLPDPMDSDHAGLAEWVEQRLLPLRGLPPDELMPRLDALWRPLDAHGRLVLFKLITGAFRVGVSRLLVTRAL
GEVAGIDPKRVAERMVGYTDLSARPDATRFQALLAPESEDDRELRANGQPFPFFLAHPLQVPLEQFDAVLGDPDNWQVEW
KWDGIRAQLVCRGNQTWLWSRGEELITERFPEIVEAAAPLPDGTVLDGEIVIWQQGRVQPFALLQQRIGRKTLNAKLLRD
APAILMAYDLLEWRGEDWRTRPQAERRTRLEQLVASHVHPALELSPLVQAGDWAHFARLREASREMGVEGFMLKAASAAY
GAGRTKDVGIWWKWKIDPFSVDAVLVYAQRGHGRRASLYTDFTFAVWNAPPGTPNRALVPFAKAYSGLTDEEMRAVDAIV
RRTTVEKFGPVRSVEPTQVFELGFEGIARSGRHKSGIAVRFPRMLRWRTDKPIDEADTLATLEAMLPVSPA

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=373, Percent_Identity=27.6139410187668, Blast_Score=94, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985269, Length=448, Percent_Identity=25.6696428571429, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6320038, Length=364, Percent_Identity=26.9230769230769, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24762424, Length=366, Percent_Identity=27.5956284153005, Blast_Score=99, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 61681; Mature: 61681

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00024 HEMOPEXIN ; PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFADLYAALDGTTSTKARLAALVDYLRAAPPQDAAWAVYFLAGGKPRQIVPVSLLRDM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECHHHHHHH
ARQAADLPDWLFEESYQAVGDLAETIALLLPDPMDSDHAGLAEWVEQRLLPLRGLPPDEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
MPRLDALWRPLDAHGRLVLFKLITGAFRVGVSRLLVTRALGEVAGIDPKRVAERMVGYTD
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
LSARPDATRFQALLAPESEDDRELRANGQPFPFFLAHPLQVPLEQFDAVLGDPDNWQVEW
CCCCCCHHHHHHEECCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEE
KWDGIRAQLVCRGNQTWLWSRGEELITERFPEIVEAAAPLPDGTVLDGEIVIWQQGRVQP
EECCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCCCH
FALLQQRIGRKTLNAKLLRDAPAILMAYDLLEWRGEDWRTRPQAERRTRLEQLVASHVHP
HHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
ALELSPLVQAGDWAHFARLREASREMGVEGFMLKAASAAYGAGRTKDVGIWWKWKIDPFS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
VDAVLVYAQRGHGRRASLYTDFTFAVWNAPPGTPNRALVPFAKAYSGLTDEEMRAVDAIV
CCEEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
RRTTVEKFGPVRSVEPTQVFELGFEGIARSGRHKSGIAVRFPRMLRWRTDKPIDEADTLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHCCCCCCCCHHHHHH
TLEAMLPVSPA
HHHHHCCCCCC
>Mature Secondary Structure
MKAFADLYAALDGTTSTKARLAALVDYLRAAPPQDAAWAVYFLAGGKPRQIVPVSLLRDM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEECHHHHHHH
ARQAADLPDWLFEESYQAVGDLAETIALLLPDPMDSDHAGLAEWVEQRLLPLRGLPPDEL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHH
MPRLDALWRPLDAHGRLVLFKLITGAFRVGVSRLLVTRALGEVAGIDPKRVAERMVGYTD
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
LSARPDATRFQALLAPESEDDRELRANGQPFPFFLAHPLQVPLEQFDAVLGDPDNWQVEW
CCCCCCHHHHHHEECCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEE
KWDGIRAQLVCRGNQTWLWSRGEELITERFPEIVEAAAPLPDGTVLDGEIVIWQQGRVQP
EECCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCCCH
FALLQQRIGRKTLNAKLLRDAPAILMAYDLLEWRGEDWRTRPQAERRTRLEQLVASHVHP
HHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
ALELSPLVQAGDWAHFARLREASREMGVEGFMLKAASAAYGAGRTKDVGIWWKWKIDPFS
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
VDAVLVYAQRGHGRRASLYTDFTFAVWNAPPGTPNRALVPFAKAYSGLTDEEMRAVDAIV
CCEEEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
RRTTVEKFGPVRSVEPTQVFELGFEGIARSGRHKSGIAVRFPRMLRWRTDKPIDEADTLA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHCCCCCCCCHHHHHH
TLEAMLPVSPA
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA