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The map label for this gene is zraR [H]

Identifier: 73537724

GI number: 73537724

Start: 512503

End: 514236

Strand: Reverse

Name: zraR [H]

Synonym: Reut_B3890

Alternate gene names: 73537724

Gene position: 514236-512503 (Counterclockwise)

Preceding gene: 73537729

Following gene: 73537723

Centisome position: 18.86

GC content: 67.01

Gene sequence:

>1734_bases
GTGTCCTCATCGACAACACAAGGGCCGACCCCGCCCGCCCTCGGCGCGGTAATCCGGTCACGCGAAGGGAAGCGGCTATC
GGCGATAGGGGTCTGTGCTGACCCCAGCACTGCGGAAGCGGCGGAAGCTGCTTGTGCCGGAGCGGCGGATCGCACGGCGG
GTAGGCGGCTGTTTCCCGTGGAGGCTGGCGGTCACCGCTACGTCGGCCTGTGTCTCGAGGACGCAGCGCAGCAGATCATC
CTGCTGCACCGCGCCGATACGCACACCGTACTGTTCGACTTCCTGGGCACGGTGCCCTTTGCCAGCGCCATCCTCAACCA
TTTCCTGACCGACCCGTACCAGGCCATCACCGTGGCTGACCGGCAAGGCCGCATGCGGTTCATGAGCCCCGTCCACGAAA
AATTCCTGGGCCTCGATACCGGCGAAGGCATCGGTCGCCCGGCCGCCGACGTCATCCCGAATTCGCGGCTGGCGCAGGTA
GTGGCCAGCGGCAAGGCCGAGATCGGGCAGCTTCAGCCGATGAATGGCGTGACGCGCGTGGTCAACCGCATTCCGATCCG
CCAGGACGGCGAGGTAGTCGGCGCCATCGGCCAGATCATGTTCAAGGGCCCGGAAGACATGGTCCGGATGCACGATGAAC
TGTCCGAACTGCGCTCGCAGGTCGCGCGCTACCAGCGCGAACTGGACGGCCTGCGCGAGGGCCAGCCGCCGCTCGGGTTG
ATCGGCGACAGCGCGCCGATGCAGCGCCTGCGCCGCGAAATCCAGACTGTTGCGCGGCTCGACGTGCCGGTGCTGATCCT
CGGGGAAAGCGGCACCGGCAAGGAACTGGTTGCCAACGCCATCCACCACGCGGGGCGCGAGCCCGTCAGCGAGCGGCCGC
TGCTCAGCCTGAACCTGGCCGCGCTGCCCGCCACGCTCATCGAGTCCGAACTGTTCGGCCATGCGCCCGGCGCGTTCACG
GGCAGCCAGCGGCATGGGCGGGCCGGCAAGCTTGAGCTCGCAGCTGGCGGCACCGTGTTCCTCGACGAGGTCGCCGACAT
CCCGATGGAGATGCAGGTCAAGTTGTTGCGCGTGTTGGAGGACCACATGGTCGAACGACTCGGCAGCCGCCGGGCGCAGC
ACGTGGACTTCCGGCTGATCTCCGCGACCAATCGCGACATTCCGGCGCTGATCGATGCGGGTCGCTTCAGGCTGGACCTG
TACTACCGCCTCAGCGGCGTGGTGCTGCGCATTCCGGCGCTGCGACAGCGGCGCGAGGATATTCCCGCGCTGCTGCAGCA
CTTCGTCAATGCGTTCTGTGCGCGCAACGGCGTGCCGGTGCCGAAGGTCGACCATGACGTCGCGCGGCACCTTGCGGGCC
AGCCGTGGCCCGGCAATGTGCGCCAGTTGCGCCAGCGTATCGAGGAAGCGCTGGTGTTCTGCGATGGCCGCATGCTCAGG
GCTACCGACTTTGCGCGTGGCGAATCGGCGCGTGGGCTCGTGGGCCTGGAAAGCGAGCCGGTCGTGCATCCACTGGCGCG
GGCGTATTCGCATGAAGAGGTTGAAACGGACAGCAGGGCATCGAATGCGGCATTGCCGGTTAGTGAGCCTGCGGCGGGGA
TGAGCGAGCTTGCCTATGCGGCAGTGGTCGACGCGATTGCGCGTCATGGCGGCAACAAGAAGCGCGCCGCAGAACAACTG
CGGATTTCGCGTTCGCATCTGTACAAGATATTGGAGCGCGGGCCCGGCGGGTGA

Upstream 100 bases:

>100_bases
GCGAGCCGTTGATTTCATTGAGGATTCTTTTTTGCGCAGCTATGATGTCCGCGATAACGACAGGATGTGTCCACACAGAG
GACACACACGGAGACAGACG

Downstream 100 bases:

>100_bases
GTCTGAACGGCGTGCGGAGACAGCAGCGCCGAGAGTTTCATCCGGCAAAATTTTTTCGCGTCGCAGGATGACGGGCATTC
TCTGATGGCCTATAACCGCT

Product: Fis-type helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MSSSTTQGPTPPALGAVIRSREGKRLSAIGVCADPSTAEAAEAACAGAADRTAGRRLFPVEAGGHRYVGLCLEDAAQQII
LLHRADTHTVLFDFLGTVPFASAILNHFLTDPYQAITVADRQGRMRFMSPVHEKFLGLDTGEGIGRPAADVIPNSRLAQV
VASGKAEIGQLQPMNGVTRVVNRIPIRQDGEVVGAIGQIMFKGPEDMVRMHDELSELRSQVARYQRELDGLREGQPPLGL
IGDSAPMQRLRREIQTVARLDVPVLILGESGTGKELVANAIHHAGREPVSERPLLSLNLAALPATLIESELFGHAPGAFT
GSQRHGRAGKLELAAGGTVFLDEVADIPMEMQVKLLRVLEDHMVERLGSRRAQHVDFRLISATNRDIPALIDAGRFRLDL
YYRLSGVVLRIPALRQRREDIPALLQHFVNAFCARNGVPVPKVDHDVARHLAGQPWPGNVRQLRQRIEEALVFCDGRMLR
ATDFARGESARGLVGLESEPVVHPLARAYSHEEVETDSRASNAALPVSEPAAGMSELAYAAVVDAIARHGGNKKRAAEQL
RISRSHLYKILERGPGG

Sequences:

>Translated_577_residues
MSSSTTQGPTPPALGAVIRSREGKRLSAIGVCADPSTAEAAEAACAGAADRTAGRRLFPVEAGGHRYVGLCLEDAAQQII
LLHRADTHTVLFDFLGTVPFASAILNHFLTDPYQAITVADRQGRMRFMSPVHEKFLGLDTGEGIGRPAADVIPNSRLAQV
VASGKAEIGQLQPMNGVTRVVNRIPIRQDGEVVGAIGQIMFKGPEDMVRMHDELSELRSQVARYQRELDGLREGQPPLGL
IGDSAPMQRLRREIQTVARLDVPVLILGESGTGKELVANAIHHAGREPVSERPLLSLNLAALPATLIESELFGHAPGAFT
GSQRHGRAGKLELAAGGTVFLDEVADIPMEMQVKLLRVLEDHMVERLGSRRAQHVDFRLISATNRDIPALIDAGRFRLDL
YYRLSGVVLRIPALRQRREDIPALLQHFVNAFCARNGVPVPKVDHDVARHLAGQPWPGNVRQLRQRIEEALVFCDGRMLR
ATDFARGESARGLVGLESEPVVHPLARAYSHEEVETDSRASNAALPVSEPAAGMSELAYAAVVDAIARHGGNKKRAAEQL
RISRSHLYKILERGPGG
>Mature_576_residues
SSSTTQGPTPPALGAVIRSREGKRLSAIGVCADPSTAEAAEAACAGAADRTAGRRLFPVEAGGHRYVGLCLEDAAQQIIL
LHRADTHTVLFDFLGTVPFASAILNHFLTDPYQAITVADRQGRMRFMSPVHEKFLGLDTGEGIGRPAADVIPNSRLAQVV
ASGKAEIGQLQPMNGVTRVVNRIPIRQDGEVVGAIGQIMFKGPEDMVRMHDELSELRSQVARYQRELDGLREGQPPLGLI
GDSAPMQRLRREIQTVARLDVPVLILGESGTGKELVANAIHHAGREPVSERPLLSLNLAALPATLIESELFGHAPGAFTG
SQRHGRAGKLELAAGGTVFLDEVADIPMEMQVKLLRVLEDHMVERLGSRRAQHVDFRLISATNRDIPALIDAGRFRLDLY
YRLSGVVLRIPALRQRREDIPALLQHFVNAFCARNGVPVPKVDHDVARHLAGQPWPGNVRQLRQRIEEALVFCDGRMLRA
TDFARGESARGLVGLESEPVVHPLARAYSHEEVETDSRASNAALPVSEPAAGMSELAYAAVVDAIARHGGNKKRAAEQLR
ISRSHLYKILERGPGG

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=337, Percent_Identity=42.433234421365, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1788905, Length=358, Percent_Identity=40.5027932960894, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082117, Length=240, Percent_Identity=45.4166666666667, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1789087, Length=244, Percent_Identity=45.4918032786885, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788550, Length=336, Percent_Identity=36.9047619047619, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789233, Length=476, Percent_Identity=29.8319327731092, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1790299, Length=337, Percent_Identity=37.6854599406528, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=44.4444444444444, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787583, Length=245, Percent_Identity=42.4489795918367, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786524, Length=247, Percent_Identity=41.7004048582996, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI87081872, Length=336, Percent_Identity=33.9285714285714, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081858, Length=478, Percent_Identity=26.3598326359833, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789828, Length=286, Percent_Identity=32.5174825174825, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62594; Mature: 62462

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTTQGPTPPALGAVIRSREGKRLSAIGVCADPSTAEAAEAACAGAADRTAGRRLFPV
CCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHCCCCEEEEE
EAGGHRYVGLCLEDAAQQIILLHRADTHTVLFDFLGTVPFASAILNHFLTDPYQAITVAD
ECCCCEEEEHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RQGRMRFMSPVHEKFLGLDTGEGIGRPAADVIPNSRLAQVVASGKAEIGQLQPMNGVTRV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHHHH
VNRIPIRQDGEVVGAIGQIMFKGPEDMVRMHDELSELRSQVARYQRELDGLREGQPPLGL
HHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IGDSAPMQRLRREIQTVARLDVPVLILGESGTGKELVANAIHHAGREPVSERPLLSLNLA
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHH
ALPATLIESELFGHAPGAFTGSQRHGRAGKLELAAGGTVFLDEVADIPMEMQVKLLRVLE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHCCCHHHHHHHHHHHH
DHMVERLGSRRAQHVDFRLISATNRDIPALIDAGRFRLDLYYRLSGVVLRIPALRQRRED
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEEEEEEECCCHHEECHHHHHHHHH
IPALLQHFVNAFCARNGVPVPKVDHDVARHLAGQPWPGNVRQLRQRIEEALVFCDGRMLR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
ATDFARGESARGLVGLESEPVVHPLARAYSHEEVETDSRASNAALPVSEPAAGMSELAYA
HHHCCCCCCCCCEECCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
AVVDAIARHGGNKKRAAEQLRISRSHLYKILERGPGG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSSTTQGPTPPALGAVIRSREGKRLSAIGVCADPSTAEAAEAACAGAADRTAGRRLFPV
CCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCHHCCCCEEEEE
EAGGHRYVGLCLEDAAQQIILLHRADTHTVLFDFLGTVPFASAILNHFLTDPYQAITVAD
ECCCCEEEEHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RQGRMRFMSPVHEKFLGLDTGEGIGRPAADVIPNSRLAQVVASGKAEIGQLQPMNGVTRV
CCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHHHH
VNRIPIRQDGEVVGAIGQIMFKGPEDMVRMHDELSELRSQVARYQRELDGLREGQPPLGL
HHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
IGDSAPMQRLRREIQTVARLDVPVLILGESGTGKELVANAIHHAGREPVSERPLLSLNLA
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEHH
ALPATLIESELFGHAPGAFTGSQRHGRAGKLELAAGGTVFLDEVADIPMEMQVKLLRVLE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCEEEHHHHHCCCHHHHHHHHHHHH
DHMVERLGSRRAQHVDFRLISATNRDIPALIDAGRFRLDLYYRLSGVVLRIPALRQRRED
HHHHHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCEEEEEEEECCCHHEECHHHHHHHHH
IPALLQHFVNAFCARNGVPVPKVDHDVARHLAGQPWPGNVRQLRQRIEEALVFCDGRMLR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
ATDFARGESARGLVGLESEPVVHPLARAYSHEEVETDSRASNAALPVSEPAAGMSELAYA
HHHCCCCCCCCCEECCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
AVVDAIARHGGNKKRAAEQLRISRSHLYKILERGPGG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]