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The map label for this gene is fadJ [H]

Identifier: 73537708

GI number: 73537708

Start: 492370

End: 494484

Strand: Direct

Name: fadJ [H]

Synonym: Reut_B3873

Alternate gene names: 73537708

Gene position: 492370-494484 (Clockwise)

Preceding gene: 73537700

Following gene: 73537709

Centisome position: 18.06

GC content: 66.29

Gene sequence:

>2115_bases
ATGACCAACGAAGCTTCCGTCGTTCGCACAGCCCGCCACGGCGACGTGCAGGTGATCACCATCTGCAATCCGCCGGTCAA
TGCCCTCGGCGTTGCCGTTCGCCAGGGCCTGAAGCAGGCCATCGAACAGGCCGAAGCCGATGCCGGCGTGCGCGCCGTGC
TGGTCGTGGGAGAAGGCACGGCCTTCATCGCCGGCGCCGATATCCGCGAATTCGGCAAGCCGCCGATGCCGCCGTCGCTG
CCGGAAGTCTGCAACCAGATCGAAGCCAGCAACAAGCTCGTTGTCGCCGCGATCCATGGTCCGGCCCTTGGGGGCGGCCT
GGAAGTCGCCATGTCGGCCCACTATCGCCTCGCGCTGCCGGCCGCCAAGCTGGGTCTGCCTGAAGTCAACCTGGGCCTGC
TGCCCGGCGCTGGCGGTACGCAACGCGCGCCGCGCCTGATGGGCGTGAAGGCCGCTGCCGATCTGATGCTCAGCGGCAAG
CACCTGTCGGCCAAGGCCGCGCAGGCCGCTGGTCTCGTTGATCGCCTGGCTGAAGGCGACGACCCTGTGGCCGCTGGCCT
CGCCTACGTCAACGAATTGCTGGAGCAAGGCGCGCCGCTGCGCCGCACGCGCGACCTCGCCATTGCCGACAAGGCCGCAG
CACTGGCCGAACTCGACACGTTGCAGGCCGAGACTGCCAAGAAGACGCGCGGCCTGTTCTCGCCGCAGAAGATTGTGGAA
TGCGTGCGTGCCGCGGTCGAACTGCCGTTCGACGAAGGCCTGGCCAAAGAGCGCGCGCTGTTCGTCGAATGCCTGAACAG
CCCGCAGCGCGCCGGCCTGGTCCATGCGTTCTTTGCCGAGCGCGAAACCGCCAAGGTGCCCGAGGCACAAGGCGCCGCCC
CGCGCGCGCTCAACCGCATCGCCGTGATCGGCGGTGGCACCATGGGCGCGGGCATCACCGTGGCCGCGCTCGATACCGGA
CTGCCGGTGGTGATGATCGAGCGCGATGCCGAATCCATCGCGCGTGGCCAGGCCAATGTCGAGAAGGTCTACGACGGCCT
GATCGCGAAGGGCCGCATGACGCCGGAAGGCAAGGCCGCGATCATGGCGCGCTACACGCCGAGCACGTCGTATGAATCGA
TTGCCGACGTGGACCTCGTGATCGAAGCCGTGTTCGAAGACATCGAAGTCAAGAAGGCCGTGTTCCAGCAACTGGACCGC
GTGTGCAAGCCGGGCGCCGTGCTGGCGACCAATACGTCGTATCTTGATATCGACGCGATCGCTGGCAGCATCTCGCGTCC
GCAGGACGTGATCGGCCTGCACTTCTTCAGCCCGGCCAACATCATGAAGCTGCTGGAGATCGTGGTGCCGGCCAAGGTCA
GCCCGGACGTGGTCGTGACCGCGTTCGAACTGGCCAAGCGCATGAAGAAGATCCCGGTGCGCGCAGGCGTGTGCGACGGC
TTTATCGGCAACCGCATCCTGGCCGTCTACAAGCAGGCCGCCGACTACATCATGGAAGACGGCGCCAGCCCTTACGAGAT
CGACGAGGCCGTGCGCGGTTTCGGCTATCCGATGGGCCCGTTCCAGGTGACCGACCTCGCCGGCGGCGACATCGGCTGGG
CCACGCGCAAGCGCCGCGCTGCCACGCGCGATCCAAAGGCGCGCTATGTCGAAGTCGCCGACCGCATCTGCGAACGCGGC
TGGTTCGGCCAGAAGACGGGCCGTGGCTTCTACCTGTACCCGCAAGGCGCACGCATCGGCCAGCCCGACCGGGAAGTGCT
CGCCATTGTCGAGGCCGAACGCCAGAAGAAGGGCATCGCACCGCGCAAGTTCACGGCCGAGGAGATCATGCGCCGCTATA
TGGCCGCGATGGTCAATGAGGGCGCCAAGGTGGTGGAAGAAGGCATCGCGCTGCGTCCGCTCGATGTCGATGTGACGTTC
CTGTCGGGCTACGGCTTCCCGCGCTTCCGCGGCGGCCCGATGAAGTATGCCGATATGGTCGGCCTGCCGAAGATCCTCGA
AGATATCCGCACCTTTGCCAAGGAAGACCCGCTATTCTGGAAGCCGGCACCGCTGCTCGAGAAGCTGGTGGCGGAAGGCA
AGGACTTTGCCAGCCTGAACCAGGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
AAATTTTATTTAACGTTATGTCAACAATCATTGTGATTGTATAGTCAATTTATCAGGGCAAGAATGCCGGACTGACCGTC
AGTAACTGGAGACAACCCCC

Downstream 100 bases:

>100_bases
CATGCCGGCGTCGCGCAGTGCGCGACGCCAGTGTGGCCAGAACAATTCATTGCAGCGAGGCGGCACATGCCTCGCTGCAG
CGCATGGAGACAAGCAATGC

Product: 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MTNEASVVRTARHGDVQVITICNPPVNALGVAVRQGLKQAIEQAEADAGVRAVLVVGEGTAFIAGADIREFGKPPMPPSL
PEVCNQIEASNKLVVAAIHGPALGGGLEVAMSAHYRLALPAAKLGLPEVNLGLLPGAGGTQRAPRLMGVKAAADLMLSGK
HLSAKAAQAAGLVDRLAEGDDPVAAGLAYVNELLEQGAPLRRTRDLAIADKAAALAELDTLQAETAKKTRGLFSPQKIVE
CVRAAVELPFDEGLAKERALFVECLNSPQRAGLVHAFFAERETAKVPEAQGAAPRALNRIAVIGGGTMGAGITVAALDTG
LPVVMIERDAESIARGQANVEKVYDGLIAKGRMTPEGKAAIMARYTPSTSYESIADVDLVIEAVFEDIEVKKAVFQQLDR
VCKPGAVLATNTSYLDIDAIAGSISRPQDVIGLHFFSPANIMKLLEIVVPAKVSPDVVVTAFELAKRMKKIPVRAGVCDG
FIGNRILAVYKQAADYIMEDGASPYEIDEAVRGFGYPMGPFQVTDLAGGDIGWATRKRRAATRDPKARYVEVADRICERG
WFGQKTGRGFYLYPQGARIGQPDREVLAIVEAERQKKGIAPRKFTAEEIMRRYMAAMVNEGAKVVEEGIALRPLDVDVTF
LSGYGFPRFRGGPMKYADMVGLPKILEDIRTFAKEDPLFWKPAPLLEKLVAEGKDFASLNQAGR

Sequences:

>Translated_704_residues
MTNEASVVRTARHGDVQVITICNPPVNALGVAVRQGLKQAIEQAEADAGVRAVLVVGEGTAFIAGADIREFGKPPMPPSL
PEVCNQIEASNKLVVAAIHGPALGGGLEVAMSAHYRLALPAAKLGLPEVNLGLLPGAGGTQRAPRLMGVKAAADLMLSGK
HLSAKAAQAAGLVDRLAEGDDPVAAGLAYVNELLEQGAPLRRTRDLAIADKAAALAELDTLQAETAKKTRGLFSPQKIVE
CVRAAVELPFDEGLAKERALFVECLNSPQRAGLVHAFFAERETAKVPEAQGAAPRALNRIAVIGGGTMGAGITVAALDTG
LPVVMIERDAESIARGQANVEKVYDGLIAKGRMTPEGKAAIMARYTPSTSYESIADVDLVIEAVFEDIEVKKAVFQQLDR
VCKPGAVLATNTSYLDIDAIAGSISRPQDVIGLHFFSPANIMKLLEIVVPAKVSPDVVVTAFELAKRMKKIPVRAGVCDG
FIGNRILAVYKQAADYIMEDGASPYEIDEAVRGFGYPMGPFQVTDLAGGDIGWATRKRRAATRDPKARYVEVADRICERG
WFGQKTGRGFYLYPQGARIGQPDREVLAIVEAERQKKGIAPRKFTAEEIMRRYMAAMVNEGAKVVEEGIALRPLDVDVTF
LSGYGFPRFRGGPMKYADMVGLPKILEDIRTFAKEDPLFWKPAPLLEKLVAEGKDFASLNQAGR
>Mature_703_residues
TNEASVVRTARHGDVQVITICNPPVNALGVAVRQGLKQAIEQAEADAGVRAVLVVGEGTAFIAGADIREFGKPPMPPSLP
EVCNQIEASNKLVVAAIHGPALGGGLEVAMSAHYRLALPAAKLGLPEVNLGLLPGAGGTQRAPRLMGVKAAADLMLSGKH
LSAKAAQAAGLVDRLAEGDDPVAAGLAYVNELLEQGAPLRRTRDLAIADKAAALAELDTLQAETAKKTRGLFSPQKIVEC
VRAAVELPFDEGLAKERALFVECLNSPQRAGLVHAFFAERETAKVPEAQGAAPRALNRIAVIGGGTMGAGITVAALDTGL
PVVMIERDAESIARGQANVEKVYDGLIAKGRMTPEGKAAIMARYTPSTSYESIADVDLVIEAVFEDIEVKKAVFQQLDRV
CKPGAVLATNTSYLDIDAIAGSISRPQDVIGLHFFSPANIMKLLEIVVPAKVSPDVVVTAFELAKRMKKIPVRAGVCDGF
IGNRILAVYKQAADYIMEDGASPYEIDEAVRGFGYPMGPFQVTDLAGGDIGWATRKRRAATRDPKARYVEVADRICERGW
FGQKTGRGFYLYPQGARIGQPDREVLAIVEAERQKKGIAPRKFTAEEIMRRYMAAMVNEGAKVVEEGIALRPLDVDVTFL
SGYGFPRFRGGPMKYADMVGLPKILEDIRTFAKEDPLFWKPAPLLEKLVAEGKDFASLNQAGR

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI68989263, Length=719, Percent_Identity=39.221140472879, Blast_Score=499, Evalue=1e-141,
Organism=Homo sapiens, GI261878539, Length=626, Percent_Identity=39.776357827476, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI20127408, Length=718, Percent_Identity=30.6406685236769, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI296179429, Length=292, Percent_Identity=33.2191780821918, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI296179427, Length=309, Percent_Identity=30.7443365695793, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI194097323, Length=279, Percent_Identity=28.673835125448, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI4502327, Length=193, Percent_Identity=36.2694300518135, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI70995211, Length=168, Percent_Identity=26.7857142857143, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788682, Length=716, Percent_Identity=32.6815642458101, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1790281, Length=682, Percent_Identity=30.6451612903226, Blast_Score=291, Evalue=1e-79,
Organism=Escherichia coli, GI1787661, Length=279, Percent_Identity=38.7096774193548, Blast_Score=176, Evalue=7e-45,
Organism=Escherichia coli, GI1787659, Length=276, Percent_Identity=30.4347826086957, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI221142681, Length=180, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=736, Percent_Identity=31.1141304347826, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17508951, Length=729, Percent_Identity=31.6872427983539, Blast_Score=318, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17508953, Length=729, Percent_Identity=31.6872427983539, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25144276, Length=606, Percent_Identity=32.3432343234323, Blast_Score=275, Evalue=9e-74,
Organism=Caenorhabditis elegans, GI71985923, Length=379, Percent_Identity=31.1345646437995, Blast_Score=231, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71985930, Length=382, Percent_Identity=29.5811518324607, Blast_Score=227, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17553560, Length=300, Percent_Identity=31.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17563036, Length=287, Percent_Identity=33.1010452961672, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17549919, Length=292, Percent_Identity=30.8219178082192, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=31.5018315018315, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=176, Percent_Identity=33.5227272727273, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=28.8372093023256, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=185, Percent_Identity=31.3513513513514, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921000, Length=731, Percent_Identity=33.6525307797538, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24583077, Length=731, Percent_Identity=33.6525307797538, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24583079, Length=731, Percent_Identity=33.6525307797538, Blast_Score=315, Evalue=7e-86,
Organism=Drosophila melanogaster, GI20129971, Length=276, Percent_Identity=29.3478260869565, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653477, Length=276, Percent_Identity=29.3478260869565, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24653139, Length=211, Percent_Identity=31.7535545023697, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583165, Length=190, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 75265; Mature: 75134

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEASVVRTARHGDVQVITICNPPVNALGVAVRQGLKQAIEQAEADAGVRAVLVVGEGT
CCCCCCHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
AFIAGADIREFGKPPMPPSLPEVCNQIEASNKLVVAAIHGPALGGGLEVAMSAHYRLALP
EEEECCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCEEEECC
AAKLGLPEVNLGLLPGAGGTQRAPRLMGVKAAADLMLSGKHLSAKAAQAAGLVDRLAEGD
HHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DPVAAGLAYVNELLEQGAPLRRTRDLAIADKAAALAELDTLQAETAKKTRGLFSPQKIVE
CHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
CVRAAVELPFDEGLAKERALFVECLNSPQRAGLVHAFFAERETAKVPEAQGAAPRALNRI
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHCCHHCCCCCCCCCCCHHHCEE
AVIGGGTMGAGITVAALDTGLPVVMIERDAESIARGQANVEKVYDGLIAKGRMTPEGKAA
EEEECCCCCCCEEEEEECCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEE
IMARYTPSTSYESIADVDLVIEAVFEDIEVKKAVFQQLDRVCKPGAVLATNTSYLDIDAI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHH
AGSISRPQDVIGLHFFSPANIMKLLEIVVPAKVSPDVVVTAFELAKRMKKIPVRAGVCDG
HCCCCCCCHHHEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FIGNRILAVYKQAADYIMEDGASPYEIDEAVRGFGYPMGPFQVTDLAGGDIGWATRKRRA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHC
ATRDPKARYVEVADRICERGWFGQKTGRGFYLYPQGARIGQPDREVLAIVEAERQKKGIA
CCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
PRKFTAEEIMRRYMAAMVNEGAKVVEEGIALRPLDVDVTFLSGYGFPRFRGGPMKYADMV
CCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHC
GLPKILEDIRTFAKEDPLFWKPAPLLEKLVAEGKDFASLNQAGR
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure 
TNEASVVRTARHGDVQVITICNPPVNALGVAVRQGLKQAIEQAEADAGVRAVLVVGEGT
CCCCCHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
AFIAGADIREFGKPPMPPSLPEVCNQIEASNKLVVAAIHGPALGGGLEVAMSAHYRLALP
EEEECCCHHHCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHCCEEEECC
AAKLGLPEVNLGLLPGAGGTQRAPRLMGVKAAADLMLSGKHLSAKAAQAAGLVDRLAEGD
HHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
DPVAAGLAYVNELLEQGAPLRRTRDLAIADKAAALAELDTLQAETAKKTRGLFSPQKIVE
CHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
CVRAAVELPFDEGLAKERALFVECLNSPQRAGLVHAFFAERETAKVPEAQGAAPRALNRI
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHHCCHHCCCCCCCCCCCHHHCEE
AVIGGGTMGAGITVAALDTGLPVVMIERDAESIARGQANVEKVYDGLIAKGRMTPEGKAA
EEEECCCCCCCEEEEEECCCCCEEEEECCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEE
IMARYTPSTSYESIADVDLVIEAVFEDIEVKKAVFQQLDRVCKPGAVLATNTSYLDIDAI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEHHHH
AGSISRPQDVIGLHFFSPANIMKLLEIVVPAKVSPDVVVTAFELAKRMKKIPVRAGVCDG
HCCCCCCCHHHEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
FIGNRILAVYKQAADYIMEDGASPYEIDEAVRGFGYPMGPFQVTDLAGGDIGWATRKRRA
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHC
ATRDPKARYVEVADRICERGWFGQKTGRGFYLYPQGARIGQPDREVLAIVEAERQKKGIA
CCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
PRKFTAEEIMRRYMAAMVNEGAKVVEEGIALRPLDVDVTFLSGYGFPRFRGGPMKYADMV
CCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHC
GLPKILEDIRTFAKEDPLFWKPAPLLEKLVAEGKDFASLNQAGR
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA