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The map label for this gene is iclR [H]

Identifier: 73537589

GI number: 73537589

Start: 351364

End: 352191

Strand: Direct

Name: iclR [H]

Synonym: Reut_B3754

Alternate gene names: 73537589

Gene position: 351364-352191 (Clockwise)

Preceding gene: 73537582

Following gene: 73537590

Centisome position: 12.89

GC content: 68.24

Gene sequence:

>828_bases
ATGAACGAATTGCGTCTTGCCAAGAGCGATGCCAGGCCTGACGGCGACACGCCGGCGCTGCGTCTCTTTGGCCTGCTGGA
AGTCATTGCCACCAAGGATCACCCCTTCAACCTGCAGGCGCTGGTGGAGGAAACCGGCCTGCCCAAGCCCACGCTGCACC
GCATGCTGCAGCAGCTCGAAGCGGCCGGCATGGTCCAGCGCAACGGCGATGGCCGCCACTACGGCACCGGCCTGCGCCTG
CGGCGGCTGGCGGAAAACCTGTTGCTGAACAACACCTCGCACGGCGCGCGCCACATGGTGCTGCGCAAGCTGGTGGAAGA
GGTCGGCGAAAGCTGCAACCTGACCGCGTTTTCGAGCGGCGAAGTGCTGTACCTGGACCGCGTGGAAACAGCGGCGCCCT
TGCGCTTCTACCTGCACCCCGGCTCGCGCGTGCCGGCGCACTGCTCGGCCACCGGCAAGCTGTTCCTGGCGCAATTGACG
CCAGCACAGCGCCAGCGCCTGCTCGCGCATGCGGAGCTGGAACGCTACACGGCCAACACGCTGACCGACCACGTGGAGCT
GGAAGCGGAGATCGAGCGCGTGAAGCGCGACGGCTACGCGATGGATGACGAAGAGTTCCTGCCCGGCCTGCTGTGCATCG
GCGTGCTGGTGCCGGCGGTGGACGGCGGGAAATCCAACCTGGGGCTCGCGTTGCAGGCCCCGGTGATGCGCGTGACGCAC
GAAAAGGCGCTGCAGATGCTGCCCGCGCTGCAGCGCGCGGCCAGGGCGCTGGCGGCCATCGAGGCCGAGAGCGGTACCGC
CTGGGGCGGCGCGGACGAGGACGAGTAG

Upstream 100 bases:

>100_bases
TCGGAATGTAGGTCTTCCTGAGCCGAAGGTCAACCCGGAATTTCAGGAATTGATATGATTTGTATCTGAAACTGAAACTT
GACTATGATGGGAGCCAGGC

Downstream 100 bases:

>100_bases
CGGCGGATCGCGCAGGCTCCCGGCTTGTCTGCTGTCCGCCACAAGAAGGACACAAGCAAGGCAAGCAAGAGGAGCAAGAC
GTGACGCAGAAGGAGTTTGG

Product: regulatory protein IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNELRLAKSDARPDGDTPALRLFGLLEVIATKDHPFNLQALVEETGLPKPTLHRMLQQLEAAGMVQRNGDGRHYGTGLRL
RRLAENLLLNNTSHGARHMVLRKLVEEVGESCNLTAFSSGEVLYLDRVETAAPLRFYLHPGSRVPAHCSATGKLFLAQLT
PAQRQRLLAHAELERYTANTLTDHVELEAEIERVKRDGYAMDDEEFLPGLLCIGVLVPAVDGGKSNLGLALQAPVMRVTH
EKALQMLPALQRAARALAAIEAESGTAWGGADEDE

Sequences:

>Translated_275_residues
MNELRLAKSDARPDGDTPALRLFGLLEVIATKDHPFNLQALVEETGLPKPTLHRMLQQLEAAGMVQRNGDGRHYGTGLRL
RRLAENLLLNNTSHGARHMVLRKLVEEVGESCNLTAFSSGEVLYLDRVETAAPLRFYLHPGSRVPAHCSATGKLFLAQLT
PAQRQRLLAHAELERYTANTLTDHVELEAEIERVKRDGYAMDDEEFLPGLLCIGVLVPAVDGGKSNLGLALQAPVMRVTH
EKALQMLPALQRAARALAAIEAESGTAWGGADEDE
>Mature_275_residues
MNELRLAKSDARPDGDTPALRLFGLLEVIATKDHPFNLQALVEETGLPKPTLHRMLQQLEAAGMVQRNGDGRHYGTGLRL
RRLAENLLLNNTSHGARHMVLRKLVEEVGESCNLTAFSSGEVLYLDRVETAAPLRFYLHPGSRVPAHCSATGKLFLAQLT
PAQRQRLLAHAELERYTANTLTDHVELEAEIERVKRDGYAMDDEEFLPGLLCIGVLVPAVDGGKSNLGLALQAPVMRVTH
EKALQMLPALQRAARALAAIEAESGTAWGGADEDE

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=253, Percent_Identity=29.6442687747036, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788131, Length=254, Percent_Identity=27.9527559055118, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789999, Length=262, Percent_Identity=27.0992366412214, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786715, Length=212, Percent_Identity=25, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786468, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788581, Length=207, Percent_Identity=26.0869565217391, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790752, Length=221, Percent_Identity=23.5294117647059, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30120; Mature: 30120

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELRLAKSDARPDGDTPALRLFGLLEVIATKDHPFNLQALVEETGLPKPTLHRMLQQLE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
AAGMVQRNGDGRHYGTGLRLRRLAENLLLNNTSHGARHMVLRKLVEEVGESCNLTAFSSG
HHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
EVLYLDRVETAAPLRFYLHPGSRVPAHCSATGKLFLAQLTPAQRQRLLAHAELERYTANT
CEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LTDHVELEAEIERVKRDGYAMDDEEFLPGLLCIGVLVPAVDGGKSNLGLALQAPVMRVTH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHH
EKALQMLPALQRAARALAAIEAESGTAWGGADEDE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNELRLAKSDARPDGDTPALRLFGLLEVIATKDHPFNLQALVEETGLPKPTLHRMLQQLE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
AAGMVQRNGDGRHYGTGLRLRRLAENLLLNNTSHGARHMVLRKLVEEVGESCNLTAFSSG
HHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
EVLYLDRVETAAPLRFYLHPGSRVPAHCSATGKLFLAQLTPAQRQRLLAHAELERYTANT
CEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LTDHVELEAEIERVKRDGYAMDDEEFLPGLLCIGVLVPAVDGGKSNLGLALQAPVMRVTH
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHH
EKALQMLPALQRAARALAAIEAESGTAWGGADEDE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]