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The map label for this gene is phaZ1 [H]

Identifier: 73537461

GI number: 73537461

Start: 195600

End: 196688

Strand: Direct

Name: phaZ1 [H]

Synonym: Reut_B3626

Alternate gene names: 73537461

Gene position: 195600-196688 (Clockwise)

Preceding gene: 73537459

Following gene: 73537463

Centisome position: 7.17

GC content: 68.32

Gene sequence:

>1089_bases
ATGGCCAAGAGCCTCGCCACGGACTGGCAGGCGCAATTCCGGCGAATGAGCCGGGCGCAGGCCCAGACCGAAGCACAAGT
GAAATCGTGGCTGGAACGCATTGAATCGTTGAGCCCGCTGGCGCCACGGCGCCGGATCCGGACACCGCATGGTGTCCCGC
TTTCTCCGCGGTACCCTGTGCCGCAATTGCCGGGCAGCTGGGAATCGCACCGGCTGCGCCTGGCGCCGCAGCCCGGCGAA
CTGGTGCCGCAGCTTTCCTATCACCTTTACGTACCGGCCAGCGCCAAACACCGCTTTCGCGGCCCATTGCCCGTGGTGGT
GATGCTGCATGGCTGCCGCCAGACTCCCGACGACCTCGCCGCGGGCACACGCATGAATGCGCTGGCCGAGCGAGAGGGAT
TTATCGTGGCCTATCCGCAGCAGCCGCTGCGGCGCCAACTCCAGCGCTGCTGGCAGTGGTTCGACCTTGCCGCCGGTGAA
GGCGGCCGTGAAGCACAGGCGGTTGCCGCGCTGATCGATGCGCTGGCACGGCGCCACGACGTGCGTGAGGACCAGGTCTA
CCTTGCTGGGATGTCAGCGGGCGCTGCCATGGCCGCCGTGGTTGCGCTGCGCTATCCGGAGCGCGTGGCGGCAGTCGGCC
TGCACTCCGGGGTGGTGGTGGGAGCGGCCGATAATCCGGGGGCGGGCCTCCGGGCCATGCTCCATGGGTCGGAGGCCGAT
CCGGCGTGGCTGGTCGACGCGGTTGGCGCCACGCCCGGCGGTCCCGAACTGCCGGCAATCGTCGTGCACGGGCTTGACGA
CGACGCGGTGCATCCGGTCAACGGGCGCCTGCTGGCACGGCAGTTCCTGGCCTACAACCGCCTGGAAGACCGGCTGCGCG
GTATCGAAGAGGCCGACATCGACTTGCCGGCCGGCCACTTTCACGAGGCGCGCTTCGGGCGCTGGAGCCGCGACCTTGTC
ACACTCGTCGAAGTCGAAGGGCTGGGACACGCGTGGAGCGGAGGGGATGGCGACTACAGCTATCACAGCGCCACCGGTCC
CGATGCCAGCGCGATGATGTGGGAATTCTTTCGCGAACACAGCCGGTAG

Upstream 100 bases:

>100_bases
AGCCACGCTGCGGGGCAAGTCTGCAGTGGCTTGTGATATGCTGCATTGCAACAACATGAAGCACCCGGGGGAGACACGTC
TCAGATGACCAGGAGTTCTC

Downstream 100 bases:

>100_bases
CCGGCGACCATGAAAAAGGAGGGCAGGCAGCCCTCCTTGATGTTGTGGCAACTTGCCGCAGTGCCTGGCAACTCGCCGCT
GCGCATCAGCCGCGCGGGCG

Product: esterase, PHB depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAKSLATDWQAQFRRMSRAQAQTEAQVKSWLERIESLSPLAPRRRIRTPHGVPLSPRYPVPQLPGSWESHRLRLAPQPGE
LVPQLSYHLYVPASAKHRFRGPLPVVVMLHGCRQTPDDLAAGTRMNALAEREGFIVAYPQQPLRRQLQRCWQWFDLAAGE
GGREAQAVAALIDALARRHDVREDQVYLAGMSAGAAMAAVVALRYPERVAAVGLHSGVVVGAADNPGAGLRAMLHGSEAD
PAWLVDAVGATPGGPELPAIVVHGLDDDAVHPVNGRLLARQFLAYNRLEDRLRGIEEADIDLPAGHFHEARFGRWSRDLV
TLVEVEGLGHAWSGGDGDYSYHSATGPDASAMMWEFFREHSR

Sequences:

>Translated_362_residues
MAKSLATDWQAQFRRMSRAQAQTEAQVKSWLERIESLSPLAPRRRIRTPHGVPLSPRYPVPQLPGSWESHRLRLAPQPGE
LVPQLSYHLYVPASAKHRFRGPLPVVVMLHGCRQTPDDLAAGTRMNALAEREGFIVAYPQQPLRRQLQRCWQWFDLAAGE
GGREAQAVAALIDALARRHDVREDQVYLAGMSAGAAMAAVVALRYPERVAAVGLHSGVVVGAADNPGAGLRAMLHGSEAD
PAWLVDAVGATPGGPELPAIVVHGLDDDAVHPVNGRLLARQFLAYNRLEDRLRGIEEADIDLPAGHFHEARFGRWSRDLV
TLVEVEGLGHAWSGGDGDYSYHSATGPDASAMMWEFFREHSR
>Mature_361_residues
AKSLATDWQAQFRRMSRAQAQTEAQVKSWLERIESLSPLAPRRRIRTPHGVPLSPRYPVPQLPGSWESHRLRLAPQPGEL
VPQLSYHLYVPASAKHRFRGPLPVVVMLHGCRQTPDDLAAGTRMNALAEREGFIVAYPQQPLRRQLQRCWQWFDLAAGEG
GREAQAVAALIDALARRHDVREDQVYLAGMSAGAAMAAVVALRYPERVAAVGLHSGVVVGAADNPGAGLRAMLHGSEADP
AWLVDAVGATPGGPELPAIVVHGLDDDAVHPVNGRLLARQFLAYNRLEDRLRGIEEADIDLPAGHFHEARFGRWSRDLVT
LVEVEGLGHAWSGGDGDYSYHSATGPDASAMMWEFFREHSR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39781; Mature: 39649

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSLATDWQAQFRRMSRAQAQTEAQVKSWLERIESLSPLAPRRRIRTPHGVPLSPRYPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCC
PQLPGSWESHRLRLAPQPGELVPQLSYHLYVPASAKHRFRGPLPVVVMLHGCRQTPDDLA
CCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHH
AGTRMNALAEREGFIVAYPQQPLRRQLQRCWQWFDLAAGEGGREAQAVAALIDALARRHD
CCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
VREDQVYLAGMSAGAAMAAVVALRYPERVAAVGLHSGVVVGAADNPGAGLRAMLHGSEAD
CCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCC
PAWLVDAVGATPGGPELPAIVVHGLDDDAVHPVNGRLLARQFLAYNRLEDRLRGIEEADI
CHHEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DLPAGHFHEARFGRWSRDLVTLVEVEGLGHAWSGGDGDYSYHSATGPDASAMMWEFFREH
CCCCCCCHHHHCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SR
CC
>Mature Secondary Structure 
AKSLATDWQAQFRRMSRAQAQTEAQVKSWLERIESLSPLAPRRRIRTPHGVPLSPRYPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCC
PQLPGSWESHRLRLAPQPGELVPQLSYHLYVPASAKHRFRGPLPVVVMLHGCRQTPDDLA
CCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHEECCCCCCHHHHH
AGTRMNALAEREGFIVAYPQQPLRRQLQRCWQWFDLAAGEGGREAQAVAALIDALARRHD
CCCHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
VREDQVYLAGMSAGAAMAAVVALRYPERVAAVGLHSGVVVGAADNPGAGLRAMLHGSEAD
CCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCC
PAWLVDAVGATPGGPELPAIVVHGLDDDAVHPVNGRLLARQFLAYNRLEDRLRGIEEADI
CHHEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DLPAGHFHEARFGRWSRDLVTLVEVEGLGHAWSGGDGDYSYHSATGPDASAMMWEFFREH
CCCCCCCHHHHCCCCHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]