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The map label for this gene is 73537444

Identifier: 73537444

GI number: 73537444

Start: 171739

End: 172710

Strand: Direct

Name: 73537444

Synonym: Reut_B3609

Alternate gene names: NA

Gene position: 171739-172710 (Clockwise)

Preceding gene: 73537441

Following gene: 73537445

Centisome position: 6.3

GC content: 66.26

Gene sequence:

>972_bases
ATGAAGACACGTATCACCGAAATGCTGGGTATCCGTTACCCGATCATCCAGGGCGGCATGCAATGGGTTGGTTATGCCGA
GATGGCAGCCGCTGTCTCCAATGCGGGCGGCCTGGGTATCCTGACCGCGCTGACGCAGCCGACGCCGGAGGCGCTGGCCG
AAGAAATCCGCCGCTGCCGCGAGATGACGGACCGTCCGTTCGGCGTCAACCTCACGCTGCTGCCGTCGATCAACCCGCCG
CCGTACGCGCGCTACCTCGATGTCATCATCGAAAGCGGCGTGAAGGTGCTGGAAACCGCCGGCAACAACCCCAAGGAACA
CATTGCGCGCGCCAAGGCCGCCGGCATGAAGGTGATCCACAAATGCGTGGCGATCCGTCATGCGCTGTCGGCCGAGCGGC
TGGGGGTGGACGCCGTGTCGATCGATGGCTTCGAGTGCGCGGGCCACCCGGGCGAGGACGACGTTCCGGGCATGGTGCTG
ATTCCGCTGGCGGCGCGCAAGCTCAGTATTCCTGTCATCGCTTCGGGCGGTATCGCCGATGGCCGTGGCATGGCGGCGGC
CCTGGTGCTGGGCGCCGAGGGCGTGAACATGGGCACGCGTTTCTGCGCCACGCAGGAAGCGCCGATCCACGACAACATCA
AGCAGGCGCTGGTAGCCGCCAGCGAGCGCGATACCAACCTGATCTTCCGCACCATGCACAACACCGCGCGCGTGCTGAAG
AACGCCATATCGGACGAGGTGGTCAGCATCGAACGCCGCCCGGGCGGTGCACAGTTCGAGGAAGTGCGCCACCTGGTCAC
GGGGCAGCGCGGCAAGGCGGCCTTGGTGGCTGGTGAGCCCGATGGCGGCATCGTCAGCGCGGGCCAGTGCGTGGGCCTGA
TCGACGATGTGCCGAGCTGCGAGGAGTTGGTCGCGCGCATGGTGGCGGAGTGTCGCGAACAGCTTGGCACGGCCTCGCGC
TATTTCGGCTGA

Upstream 100 bases:

>100_bases
TTCCGACGAAGCTGGCGCGCACGGCGGCATCTTAGACTCCCACTGCGTGGCGCGGCCTGCCCCAAGGCGGCAAGGCGCGC
GGCTGAAAACAAGGACGACC

Downstream 100 bases:

>100_bases
AGCCATGGCGGAAGCGGACATTGCTGCCATGTCGACCGGCGAGGATGTGGAAACCATGCCCGAAGGCTTCGTGGCCCTGC
GCCAGCTGGACGGCTATATG

Product: 2-nitropropane dioxygenase

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKTRITEMLGIRYPIIQGGMQWVGYAEMAAAVSNAGGLGILTALTQPTPEALAEEIRRCREMTDRPFGVNLTLLPSINPP
PYARYLDVIIESGVKVLETAGNNPKEHIARAKAAGMKVIHKCVAIRHALSAERLGVDAVSIDGFECAGHPGEDDVPGMVL
IPLAARKLSIPVIASGGIADGRGMAAALVLGAEGVNMGTRFCATQEAPIHDNIKQALVAASERDTNLIFRTMHNTARVLK
NAISDEVVSIERRPGGAQFEEVRHLVTGQRGKAALVAGEPDGGIVSAGQCVGLIDDVPSCEELVARMVAECREQLGTASR
YFG

Sequences:

>Translated_323_residues
MKTRITEMLGIRYPIIQGGMQWVGYAEMAAAVSNAGGLGILTALTQPTPEALAEEIRRCREMTDRPFGVNLTLLPSINPP
PYARYLDVIIESGVKVLETAGNNPKEHIARAKAAGMKVIHKCVAIRHALSAERLGVDAVSIDGFECAGHPGEDDVPGMVL
IPLAARKLSIPVIASGGIADGRGMAAALVLGAEGVNMGTRFCATQEAPIHDNIKQALVAASERDTNLIFRTMHNTARVLK
NAISDEVVSIERRPGGAQFEEVRHLVTGQRGKAALVAGEPDGGIVSAGQCVGLIDDVPSCEELVARMVAECREQLGTASR
YFG
>Mature_323_residues
MKTRITEMLGIRYPIIQGGMQWVGYAEMAAAVSNAGGLGILTALTQPTPEALAEEIRRCREMTDRPFGVNLTLLPSINPP
PYARYLDVIIESGVKVLETAGNNPKEHIARAKAAGMKVIHKCVAIRHALSAERLGVDAVSIDGFECAGHPGEDDVPGMVL
IPLAARKLSIPVIASGGIADGRGMAAALVLGAEGVNMGTRFCATQEAPIHDNIKQALVAASERDTNLIFRTMHNTARVLK
NAISDEVVSIERRPGGAQFEEVRHLVTGQRGKAALVAGEPDGGIVSAGQCVGLIDDVPSCEELVARMVAECREQLGTASR
YFG

Specific function: Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 34238; Mature: 34238

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRITEMLGIRYPIIQGGMQWVGYAEMAAAVSNAGGLGILTALTQPTPEALAEEIRRCR
CCCHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHH
EMTDRPFGVNLTLLPSINPPPYARYLDVIIESGVKVLETAGNNPKEHIARAKAAGMKVIH
HHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHH
KCVAIRHALSAERLGVDAVSIDGFECAGHPGEDDVPGMVLIPLAARKLSIPVIASGGIAD
HHHHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCEEEEEECHHHCCCCEEECCCCCC
GRGMAAALVLGAEGVNMGTRFCATQEAPIHDNIKQALVAASERDTNLIFRTMHNTARVLK
CCCHHHHEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NAISDEVVSIERRPGGAQFEEVRHLVTGQRGKAALVAGEPDGGIVSAGQCVGLIDDVPSC
HHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCEEEHHCCCCCH
EELVARMVAECREQLGTASRYFG
HHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKTRITEMLGIRYPIIQGGMQWVGYAEMAAAVSNAGGLGILTALTQPTPEALAEEIRRCR
CCCHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHH
EMTDRPFGVNLTLLPSINPPPYARYLDVIIESGVKVLETAGNNPKEHIARAKAAGMKVIH
HHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHH
KCVAIRHALSAERLGVDAVSIDGFECAGHPGEDDVPGMVLIPLAARKLSIPVIASGGIAD
HHHHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCEEEEEECHHHCCCCEEECCCCCC
GRGMAAALVLGAEGVNMGTRFCATQEAPIHDNIKQALVAASERDTNLIFRTMHNTARVLK
CCCHHHHEEECCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
NAISDEVVSIERRPGGAQFEEVRHLVTGQRGKAALVAGEPDGGIVSAGQCVGLIDDVPSC
HHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCEEECCCEEEHHCCCCCH
EELVARMVAECREQLGTASRYFG
HHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]