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The map label for this gene is menE [H]

Identifier: 73537440

GI number: 73537440

Start: 165506

End: 167050

Strand: Direct

Name: menE [H]

Synonym: Reut_B3605

Alternate gene names: 73537440

Gene position: 165506-167050 (Clockwise)

Preceding gene: 73537434

Following gene: 73537441

Centisome position: 6.07

GC content: 64.4

Gene sequence:

>1545_bases
ATGCACCCGTACGTCCACGCGCAGCGCACCCCAGACAAGCCGGCCGTCATCATGGGCGACGGCACTGTGGTGACCTACCG
CGAACTGGATGAGCATTCCAACCAGGTGGCTCGTCTCTATCGCGACCACGGCTTGCAGCCCGGCGATCGCGTGGCGTTCC
TGATCGAAAACCACCCGCGGCTGTTCGAACTGTGCTGGGGCGCGCAACGCAGCGGCATTACGTTCATCTGCCTGAGCACG
CGCCTGAACGTGGCCGACGCCGCCTATATCGTTCGGGACAGCGGCGCGCAGATGCTCATCACGTCGCGCGCGCAAGCGGA
GATTGCTGCCGCCCTGACGGAGCAGGTGCCCGGTCTGAAGGGCTATCTGATGCTCAACGGCACCTTGCCCGGCTACACGC
CTTACGAGACCACGCTAGCGCAATATCCGACCTGGCGCATCGACGATGAAACCACGGGCGGCGATATGCTGTATTCGTCG
GGCACGACCGGACGCCCCAAGGGCGTCTATGCGCCGCCCGCCAGCCCGCAGATCGACACGCCTACCTCGCTGACCGCGCT
GTGCCAGAAGCTGTATGGCTTCGGGCCGGACATGCGCTACCTGTCGCCAGCGCCGCTGTATCACGCCGCGCCGCTGCGCT
ACAACATGTCGGTGCAGGCACTGGGCGGCACGTCGATCGTGATGGATCATTTCGACGCGGAGCAGTTCCTGCGGCTCGTG
CAGGAACACCGTATCACGCACACGCAGGTGGTGCCGACGATGTTCTCGCGCATGCTCAAGCTGCCGGCAGAAACCCGCGG
CAGTTATGACGTGTCGTCGCTGCAGGTGGCGATCCATGCGGCGGCGCCGTGCCCGGTTCAGGTCAAGGAGCAGATGATCG
AATGGTGGGGGCCGGTGGTGTGGGAGTACTACGCCGGCACTGAAGGCAATGGCGTGACGGTGGTCAGTTCGCGGGAGTGG
GTGGAGCGCAAGGGCACGGTCGGACGTGCCATGATCGGCAAGCTGCGCATCTGCGACGCGGATGGCAAGCTGATGCCCAA
TAAGCAGCCAGGCACTATCTACTTTGCCGAGGGACGCCAGTTCGTCTACCACAATGACCCGGCCAAGACCGCGGAGTCGC
GCCACCCCGAACATGCCGAGTGGAGCACCATCGGTGACATCGGCTACGTCGACGATGACGGCTATCTCTACCTCACCGAT
CGCAAGGCCAACATGATCATCTCCGGCGGCGTGAACATCTACCCGCAGGAAGCCGAAAACCTGCTCATGACGCATCCGAA
GGTCATGGATGTGGCCGTGATCGGCGTGCCGAACGAGGATTTCGGCGAAGAGGTCAAGGCCGTGGTGCAGCCGGCCAGCA
TGGCGGACGCAGGACCGGCGCTGGCCGCGGAGCTGATTGCGTTCTGCCGGGAGAACCTGTCCGGCATCAAGTGTCCGCGC
ACTGTGGACTTCGAAGCCGAGCTGCCGCGCCTGCCAACCGGCAAGCTGCTCAAGCGCGTGCTGCGCGACCGCTACTGGGC
AGGCCACGCAAACAAGCTGGTCTGA

Upstream 100 bases:

>100_bases
CGTTTCCGACGATGTCCTAGTCTCGCGGTGGTTACCAGAATGCTCGCAGCCCGACCGCCGGCACGCGGCGGCATCCATTC
GAATAACAGGAGACCACGCC

Downstream 100 bases:

>100_bases
AAGCCCGACGCCACTGCCGCGGGCGGCTTGCCGACGCGAACGTCGGTTTACGCCATAATTCCCTGTTCATTCACAGGCAC
GGCCGCGCCGTCATGCCGGC

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MHPYVHAQRTPDKPAVIMGDGTVVTYRELDEHSNQVARLYRDHGLQPGDRVAFLIENHPRLFELCWGAQRSGITFICLST
RLNVADAAYIVRDSGAQMLITSRAQAEIAAALTEQVPGLKGYLMLNGTLPGYTPYETTLAQYPTWRIDDETTGGDMLYSS
GTTGRPKGVYAPPASPQIDTPTSLTALCQKLYGFGPDMRYLSPAPLYHAAPLRYNMSVQALGGTSIVMDHFDAEQFLRLV
QEHRITHTQVVPTMFSRMLKLPAETRGSYDVSSLQVAIHAAAPCPVQVKEQMIEWWGPVVWEYYAGTEGNGVTVVSSREW
VERKGTVGRAMIGKLRICDADGKLMPNKQPGTIYFAEGRQFVYHNDPAKTAESRHPEHAEWSTIGDIGYVDDDGYLYLTD
RKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPASMADAGPALAAELIAFCRENLSGIKCPR
TVDFEAELPRLPTGKLLKRVLRDRYWAGHANKLV

Sequences:

>Translated_514_residues
MHPYVHAQRTPDKPAVIMGDGTVVTYRELDEHSNQVARLYRDHGLQPGDRVAFLIENHPRLFELCWGAQRSGITFICLST
RLNVADAAYIVRDSGAQMLITSRAQAEIAAALTEQVPGLKGYLMLNGTLPGYTPYETTLAQYPTWRIDDETTGGDMLYSS
GTTGRPKGVYAPPASPQIDTPTSLTALCQKLYGFGPDMRYLSPAPLYHAAPLRYNMSVQALGGTSIVMDHFDAEQFLRLV
QEHRITHTQVVPTMFSRMLKLPAETRGSYDVSSLQVAIHAAAPCPVQVKEQMIEWWGPVVWEYYAGTEGNGVTVVSSREW
VERKGTVGRAMIGKLRICDADGKLMPNKQPGTIYFAEGRQFVYHNDPAKTAESRHPEHAEWSTIGDIGYVDDDGYLYLTD
RKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPASMADAGPALAAELIAFCRENLSGIKCPR
TVDFEAELPRLPTGKLLKRVLRDRYWAGHANKLV
>Mature_514_residues
MHPYVHAQRTPDKPAVIMGDGTVVTYRELDEHSNQVARLYRDHGLQPGDRVAFLIENHPRLFELCWGAQRSGITFICLST
RLNVADAAYIVRDSGAQMLITSRAQAEIAAALTEQVPGLKGYLMLNGTLPGYTPYETTLAQYPTWRIDDETTGGDMLYSS
GTTGRPKGVYAPPASPQIDTPTSLTALCQKLYGFGPDMRYLSPAPLYHAAPLRYNMSVQALGGTSIVMDHFDAEQFLRLV
QEHRITHTQVVPTMFSRMLKLPAETRGSYDVSSLQVAIHAAAPCPVQVKEQMIEWWGPVVWEYYAGTEGNGVTVVSSREW
VERKGTVGRAMIGKLRICDADGKLMPNKQPGTIYFAEGRQFVYHNDPAKTAESRHPEHAEWSTIGDIGYVDDDGYLYLTD
RKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPASMADAGPALAAELIAFCRENLSGIKCPR
TVDFEAELPRLPTGKLLKRVLRDRYWAGHANKLV

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=536, Percent_Identity=25.5597014925373, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=28.3524904214559, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI38505220, Length=524, Percent_Identity=27.2900763358779, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=26.1904761904762, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=24.5136186770428, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=25.5952380952381, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=25.5952380952381, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI42544132, Length=534, Percent_Identity=25.4681647940075, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI187761345, Length=518, Percent_Identity=24.9034749034749, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI187761343, Length=518, Percent_Identity=24.9034749034749, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI227499619, Length=471, Percent_Identity=23.9915074309979, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI38524616, Length=496, Percent_Identity=25.6048387096774, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI62865631, Length=543, Percent_Identity=23.2044198895028, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI13325055, Length=543, Percent_Identity=23.2044198895028, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI28416953, Length=548, Percent_Identity=21.7153284671533, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI227499621, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=545, Percent_Identity=27.7064220183486, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI221142682, Length=512, Percent_Identity=26.5625, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=26.1627906976744, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1790505, Length=551, Percent_Identity=20.3266787658802, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1786810, Length=509, Percent_Identity=23.3791748526523, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=502, Percent_Identity=28.8844621513944, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=28.0078895463511, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17531443, Length=525, Percent_Identity=25.1428571428571, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=544, Percent_Identity=25.7352941176471, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71994694, Length=544, Percent_Identity=25.7352941176471, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71994690, Length=544, Percent_Identity=25.7352941176471, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=527, Percent_Identity=25.8064516129032, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=25.7518796992481, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=23.1775700934579, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=385, Percent_Identity=28.8311688311688, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=24.1095890410959, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=28.1767955801105, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319264, Length=573, Percent_Identity=23.3856893542757, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21355181, Length=486, Percent_Identity=26.1316872427983, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21356441, Length=458, Percent_Identity=25.9825327510917, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=25.5859375, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581924, Length=538, Percent_Identity=22.8624535315985, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648253, Length=458, Percent_Identity=24.0174672489083, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648255, Length=458, Percent_Identity=24.0174672489083, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648257, Length=517, Percent_Identity=23.5976789168279, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656500, Length=434, Percent_Identity=24.6543778801843, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648260, Length=379, Percent_Identity=27.9683377308707, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653035, Length=502, Percent_Identity=23.5059760956175, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281365686, Length=308, Percent_Identity=26.9480519480519, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161076582, Length=509, Percent_Identity=23.5756385068762, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130357, Length=500, Percent_Identity=23.8, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550496, Length=528, Percent_Identity=24.0530303030303, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI45552801, Length=528, Percent_Identity=24.0530303030303, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21358303, Length=445, Percent_Identity=22.6966292134831, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=23.4343434343434, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI62472339, Length=546, Percent_Identity=21.4285714285714, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24667955, Length=546, Percent_Identity=21.4285714285714, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21356947, Length=359, Percent_Identity=28.133704735376, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28573631, Length=542, Percent_Identity=23.6162361623616, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20130353, Length=542, Percent_Identity=23.8007380073801, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355225, Length=374, Percent_Identity=24.331550802139, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667959, Length=391, Percent_Identity=22.2506393861893, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56900; Mature: 56900

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPYVHAQRTPDKPAVIMGDGTVVTYRELDEHSNQVARLYRDHGLQPGDRVAFLIENHPR
CCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
LFELCWGAQRSGITFICLSTRLNVADAAYIVRDSGAQMLITSRAQAEIAAALTEQVPGLK
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCE
GYLMLNGTLPGYTPYETTLAQYPTWRIDDETTGGDMLYSSGTTGRPKGVYAPPASPQIDT
EEEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCEEEECCCCCCCCCEECCCCCCCCCC
PTSLTALCQKLYGFGPDMRYLSPAPLYHAAPLRYNMSVQALGGTSIVMDHFDAEQFLRLV
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEEECCCCEEEEECCCHHHHHHHH
QEHRITHTQVVPTMFSRMLKLPAETRGSYDVSSLQVAIHAAAPCPVQVKEQMIEWWGPVV
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEE
WEYYAGTEGNGVTVVSSREWVERKGTVGRAMIGKLRICDADGKLMPNKQPGTIYFAEGRQ
EEEECCCCCCCEEEEECHHHHHHCCCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCE
FVYHNDPAKTAESRHPEHAEWSTIGDIGYVDDDGYLYLTDRKANMIISGGVNIYPQEAEN
EEEECCCHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEECCCEECCCCCCH
LLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPASMADAGPALAAELIAFCRENLSGIKCPR
HHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TVDFEAELPRLPTGKLLKRVLRDRYWAGHANKLV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHPYVHAQRTPDKPAVIMGDGTVVTYRELDEHSNQVARLYRDHGLQPGDRVAFLIENHPR
CCCCCCCCCCCCCCEEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCH
LFELCWGAQRSGITFICLSTRLNVADAAYIVRDSGAQMLITSRAQAEIAAALTEQVPGLK
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCE
GYLMLNGTLPGYTPYETTLAQYPTWRIDDETTGGDMLYSSGTTGRPKGVYAPPASPQIDT
EEEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCEEEECCCCCCCCCEECCCCCCCCCC
PTSLTALCQKLYGFGPDMRYLSPAPLYHAAPLRYNMSVQALGGTSIVMDHFDAEQFLRLV
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCEEEECCCCEEEEECCCHHHHHHHH
QEHRITHTQVVPTMFSRMLKLPAETRGSYDVSSLQVAIHAAAPCPVQVKEQMIEWWGPVV
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCEE
WEYYAGTEGNGVTVVSSREWVERKGTVGRAMIGKLRICDADGKLMPNKQPGTIYFAEGRQ
EEEECCCCCCCEEEEECHHHHHHCCCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCE
FVYHNDPAKTAESRHPEHAEWSTIGDIGYVDDDGYLYLTDRKANMIISGGVNIYPQEAEN
EEEECCCHHHHHCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEECCCEECCCCCCH
LLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPASMADAGPALAAELIAFCRENLSGIKCPR
HHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TVDFEAELPRLPTGKLLKRVLRDRYWAGHANKLV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA