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The map label for this gene is pcaR [H]

Identifier: 73537433

GI number: 73537433

Start: 157173

End: 157997

Strand: Direct

Name: pcaR [H]

Synonym: Reut_B3598

Alternate gene names: 73537433

Gene position: 157173-157997 (Clockwise)

Preceding gene: 73537432

Following gene: 73537434

Centisome position: 5.77

GC content: 66.79

Gene sequence:

>825_bases
ATGACCACCGAATCGCCGCCCGAAGACGCCCAGGACAAGTACATCGTGCCCGGGCTGGAGCGCGGCCTGCGGTTGCTGGG
GGAATTCAGCAGCCGCGAGCGCACGCTGTCGGCCGCCGAGCTGGCGCGGCGCCTGAAGGTGCCGCGCTCGACGGTTTTCC
GGCTGCTGGCCACGCTCGAGATGATGGGCTTTGTCGAACGCACTGATGGCGGCCGGGAATTCCGCCTCGGCATGGCGGTG
CTGCGGCTCGGCTTTGACTACCTCGCCTCGCTGGAACTGACCGAGCTGGGTCGTCCGCTGCTGGACCGCTTGCGCGATGA
AATCCACTACCCGTGCAATCTCGTCGTGCGCGACGGACGCTCGATCGTCTATGTCGCAAAATCCGTGGCGCCGCGCCCGT
TTGCGAGCACGGTCAACGTCGGCACGCGGCTGCCGGCGCATGCGACGGTGCTCGGACGCGTGCTGCTCGAAGACCTGTCG
CTCGACGAACTGCGCGCGCTGTATCCCGAATCGCACCTCGAAGTGTTCTCGGATAGCACGCCGCGCACGGTCGAGGAACT
CTACGACATGGTCCAGCGTGACCGCGAACGCGGCTATGTGCTGCAGGAAGGTTTCTTCGAGGCCAGCATCTCGACCATCG
CCGCGCCGGTGCGCGACCGTACTGGCAAGGTGGTGGCGGCGCTCGGGGCAACCATTCCCGCTTCGCGCATCGACCCGGAT
CAACTGGACAATATGGTGGAAATGGTCAGGGGTGCTGCCGCGGAACTGTCGCGGCTGCTCGACTACCGCCCGCCCGAACC
GCCGCGGCCGTTTGGCCTCGGCTGA

Upstream 100 bases:

>100_bases
ATATGGGACAGTTACGGATGCATGCGGCAGTGGAAAACCGCACCGCAAGGCACCCGAAGCACGTCCAGAACGCCAAAACA
CGCCATAGCAGAGACAGACC

Downstream 100 bases:

>100_bases
AGCACACAGACCATGCAGCAAAGCAACACCAGCCCCCGCAAAAAGATGTCCGTTACCTGTACCCGGCGCCGCTGGCTCAC
CACCGTGGCGACAACCCTGC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTTESPPEDAQDKYIVPGLERGLRLLGEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERTDGGREFRLGMAV
LRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVAPRPFASTVNVGTRLPAHATVLGRVLLEDLS
LDELRALYPESHLEVFSDSTPRTVEELYDMVQRDRERGYVLQEGFFEASISTIAAPVRDRTGKVVAALGATIPASRIDPD
QLDNMVEMVRGAAAELSRLLDYRPPEPPRPFGLG

Sequences:

>Translated_274_residues
MTTESPPEDAQDKYIVPGLERGLRLLGEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERTDGGREFRLGMAV
LRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVAPRPFASTVNVGTRLPAHATVLGRVLLEDLS
LDELRALYPESHLEVFSDSTPRTVEELYDMVQRDRERGYVLQEGFFEASISTIAAPVRDRTGKVVAALGATIPASRIDPD
QLDNMVEMVRGAAAELSRLLDYRPPEPPRPFGLG
>Mature_273_residues
TTESPPEDAQDKYIVPGLERGLRLLGEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERTDGGREFRLGMAVL
RLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVAPRPFASTVNVGTRLPAHATVLGRVLLEDLSL
DELRALYPESHLEVFSDSTPRTVEELYDMVQRDRERGYVLQEGFFEASISTIAAPVRDRTGKVVAALGATIPASRIDPDQ
LDNMVEMVRGAAAELSRLLDYRPPEPPRPFGLG

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=29.4354838709677, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786715, Length=245, Percent_Identity=26.9387755102041, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788131, Length=244, Percent_Identity=28.6885245901639, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87082359, Length=244, Percent_Identity=26.2295081967213, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788581, Length=255, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789999, Length=248, Percent_Identity=25.4032258064516, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30583; Mature: 30451

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTESPPEDAQDKYIVPGLERGLRLLGEFSSRERTLSAAELARRLKVPRSTVFRLLATLE
CCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MMGFVERTDGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCC
SIVYVAKSVAPRPFASTVNVGTRLPAHATVLGRVLLEDLSLDELRALYPESHLEVFSDST
EEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCC
PRTVEELYDMVQRDRERGYVLQEGFFEASISTIAAPVRDRTGKVVAALGATIPASRIDPD
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHH
QLDNMVEMVRGAAAELSRLLDYRPPEPPRPFGLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTESPPEDAQDKYIVPGLERGLRLLGEFSSRERTLSAAELARRLKVPRSTVFRLLATLE
CCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
MMGFVERTDGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGR
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCC
SIVYVAKSVAPRPFASTVNVGTRLPAHATVLGRVLLEDLSLDELRALYPESHLEVFSDST
EEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCC
PRTVEELYDMVQRDRERGYVLQEGFFEASISTIAAPVRDRTGKVVAALGATIPASRIDPD
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHH
QLDNMVEMVRGAAAELSRLLDYRPPEPPRPFGLG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]