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The map label for this gene is nifA1 [H]

Identifier: 73537425

GI number: 73537425

Start: 147986

End: 149296

Strand: Direct

Name: nifA1 [H]

Synonym: Reut_B3590

Alternate gene names: 73537425

Gene position: 147986-149296 (Clockwise)

Preceding gene: 73537422

Following gene: 73537426

Centisome position: 5.43

GC content: 66.97

Gene sequence:

>1311_bases
ATGGATCGCAAAGCCACTCTCCAGGCACTCCTGTCCTACCTGGAGCAGGAAAACCAGCCGATGATCGTCCTCGATACGGA
CTACCGCATCCTGGCTGCCAACACTGCCTACCGCCGGCAGTTCGGCGCTGTCGACCGACCCTATCTGGGCCAGCATTGCT
ACCGGATTTCGCATGGCTATGACGTACCGTGCGACCAGGCGGGCGAGCATTGCCCGATGCGCAAGGCGTTCGAGACTCGG
GGCCCGGACCGCGTCCTGCATATCCATCACACGCCGCGTGGCCCGGAACATGTGAATGTCGAACTGCGCCCGATCCTCGA
TGAGGACCAGCAGGTCGTTGCCTATGTCGAGCGCCTCAGCACAGTGCGCAGCGCATCGGCCCGCGCGAGCGCGGAAGGGC
TGGTCGGCCGCTCCGAGGCATTCAATACTGCGCTGGAGGCCGTGCAACGGGTGGCGCCTTCCTCGCTGCCGGTCCTGTTG
CTTGGCGAATCCGGCACCGGCAAGGAAATGTTTGCACGCGCTGTGCACGAGGCCAGCAGCCGCGCCGGCGGGCCGTTCGT
CGTGGTGGACTGCTCCGGCCTCACCGAGGCCCTGTTCGAGAGCGAACTCTTCGGATACGAAAAAGGCGCGTTCACGGGGG
CCATCGCGCGCAAGCCGGGTCTGGTTGAAACCGCCGATGGCGGCACGCTATTCCTCGATGAAATGGGCGACGTACCGCTG
TCGATGCAGGTCAAATTGCTGCGCCTGATCGAATCGGGGACCTACCGCCGCGTGGGTAGCGTGGAAACGCGGCGGGCGGA
CTTCCGGCTGGTCGCGGCCACGCACAAGCCACTGGACGAGATGGTCAGTCAAGGGCTGTTCCGGCAGGACCTGTACTTCC
GTATTGCCGCGTTCCCGATTCACCTGCCGGCCCTGCGCGAGCGGCTCGACGATATTCCGCTGCTTGCGGAGTCGATCCTG
CAGCGCGGGGGCGGGGCCGGGGACCAGCTCCACCTCAGCGCCGAAGCGCTGGCGCGGATGCGTGCGTATCACTGGCCCGG
CAACATCCGCGAGTTGCGCAACGTGCTCGATCGCGCCCGCCTGTACGCCGATGACGGCGTCATCCGTGCCAAGGATCTCC
CGCCGGGGCTCGTGGAAACGGGCATGGCGCCGGTCACCAGGCCGGGCCCGGCAGACGGCTGGAGAACCGACGACGAGGCG
CTGCGCGAGCGGGTCCGTCAGTTCCGGGGCACACGGCGCGACCTCGCCCAAGCCCTCGGCATGAGCGAGCGCACGCTCTA
CCGGCGGCTACGGGACCTGGGCATCCGCTGA

Upstream 100 bases:

>100_bases
CCGCGGGGCATAGCGTGGCGCTGCCACTTGGCAGTGCTGGCACAATTTGGCAGAATGGCGCGCCGCCTACGCCGTCCCCA
ACTTAGCCCCCAAGCCCCCG

Downstream 100 bases:

>100_bases
TTCGCCGATCTGGCAGCGCCGTCGCTTGACTGCCAGATCGCTGCCATTCACTGCCGCCGCTGGCAGCGGTGACATCCGCC
TGACAGCCGGAGCTGTCCTC

Product: Fis-type helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MDRKATLQALLSYLEQENQPMIVLDTDYRILAANTAYRRQFGAVDRPYLGQHCYRISHGYDVPCDQAGEHCPMRKAFETR
GPDRVLHIHHTPRGPEHVNVELRPILDEDQQVVAYVERLSTVRSASARASAEGLVGRSEAFNTALEAVQRVAPSSLPVLL
LGESGTGKEMFARAVHEASSRAGGPFVVVDCSGLTEALFESELFGYEKGAFTGAIARKPGLVETADGGTLFLDEMGDVPL
SMQVKLLRLIESGTYRRVGSVETRRADFRLVAATHKPLDEMVSQGLFRQDLYFRIAAFPIHLPALRERLDDIPLLAESIL
QRGGGAGDQLHLSAEALARMRAYHWPGNIRELRNVLDRARLYADDGVIRAKDLPPGLVETGMAPVTRPGPADGWRTDDEA
LRERVRQFRGTRRDLAQALGMSERTLYRRLRDLGIR

Sequences:

>Translated_436_residues
MDRKATLQALLSYLEQENQPMIVLDTDYRILAANTAYRRQFGAVDRPYLGQHCYRISHGYDVPCDQAGEHCPMRKAFETR
GPDRVLHIHHTPRGPEHVNVELRPILDEDQQVVAYVERLSTVRSASARASAEGLVGRSEAFNTALEAVQRVAPSSLPVLL
LGESGTGKEMFARAVHEASSRAGGPFVVVDCSGLTEALFESELFGYEKGAFTGAIARKPGLVETADGGTLFLDEMGDVPL
SMQVKLLRLIESGTYRRVGSVETRRADFRLVAATHKPLDEMVSQGLFRQDLYFRIAAFPIHLPALRERLDDIPLLAESIL
QRGGGAGDQLHLSAEALARMRAYHWPGNIRELRNVLDRARLYADDGVIRAKDLPPGLVETGMAPVTRPGPADGWRTDDEA
LRERVRQFRGTRRDLAQALGMSERTLYRRLRDLGIR
>Mature_436_residues
MDRKATLQALLSYLEQENQPMIVLDTDYRILAANTAYRRQFGAVDRPYLGQHCYRISHGYDVPCDQAGEHCPMRKAFETR
GPDRVLHIHHTPRGPEHVNVELRPILDEDQQVVAYVERLSTVRSASARASAEGLVGRSEAFNTALEAVQRVAPSSLPVLL
LGESGTGKEMFARAVHEASSRAGGPFVVVDCSGLTEALFESELFGYEKGAFTGAIARKPGLVETADGGTLFLDEMGDVPL
SMQVKLLRLIESGTYRRVGSVETRRADFRLVAATHKPLDEMVSQGLFRQDLYFRIAAFPIHLPALRERLDDIPLLAESIL
QRGGGAGDQLHLSAEALARMRAYHWPGNIRELRNVLDRARLYADDGVIRAKDLPPGLVETGMAPVTRPGPADGWRTDDEA
LRERVRQFRGTRRDLAQALGMSERTLYRRLRDLGIR

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=312, Percent_Identity=41.3461538461538, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1790437, Length=304, Percent_Identity=42.1052631578947, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=40.7624633431085, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1790299, Length=356, Percent_Identity=37.3595505617978, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=40.6832298136646, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=38.109756097561, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1788905, Length=239, Percent_Identity=43.0962343096234, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=47.1861471861472, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=234, Percent_Identity=46.1538461538462, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786524, Length=322, Percent_Identity=38.8198757763975, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787583, Length=439, Percent_Identity=31.4350797266515, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1789828, Length=289, Percent_Identity=36.3321799307958, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48532; Mature: 48532

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKATLQALLSYLEQENQPMIVLDTDYRILAANTAYRRQFGAVDRPYLGQHCYRISHGY
CCHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHCCCCCCHHHHHHEECCCCC
DVPCDQAGEHCPMRKAFETRGPDRVLHIHHTPRGPEHVNVELRPILDEDQQVVAYVERLS
CCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
TVRSASARASAEGLVGRSEAFNTALEAVQRVAPSSLPVLLLGESGTGKEMFARAVHEASS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
RAGGPFVVVDCSGLTEALFESELFGYEKGAFTGAIARKPGLVETADGGTLFLDEMGDVPL
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCH
SMQVKLLRLIESGTYRRVGSVETRRADFRLVAATHKPLDEMVSQGLFRQDLYFRIAAFPI
HHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHE
HLPALRERLDDIPLLAESILQRGGGAGDQLHLSAEALARMRAYHWPGNIRELRNVLDRAR
ECHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHH
LYADDGVIRAKDLPPGLVETGMAPVTRPGPADGWRTDDEALRERVRQFRGTRRDLAQALG
HHHCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHC
MSERTLYRRLRDLGIR
CCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDRKATLQALLSYLEQENQPMIVLDTDYRILAANTAYRRQFGAVDRPYLGQHCYRISHGY
CCHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHCCCCCCHHHHHHEECCCCC
DVPCDQAGEHCPMRKAFETRGPDRVLHIHHTPRGPEHVNVELRPILDEDQQVVAYVERLS
CCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
TVRSASARASAEGLVGRSEAFNTALEAVQRVAPSSLPVLLLGESGTGKEMFARAVHEASS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHC
RAGGPFVVVDCSGLTEALFESELFGYEKGAFTGAIARKPGLVETADGGTLFLDEMGDVPL
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEECCCCEEEEECCCCCCH
SMQVKLLRLIESGTYRRVGSVETRRADFRLVAATHKPLDEMVSQGLFRQDLYFRIAAFPI
HHHHHHHHHHHCCCCCCCCCCHHCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHE
HLPALRERLDDIPLLAESILQRGGGAGDQLHLSAEALARMRAYHWPGNIRELRNVLDRAR
ECHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHH
LYADDGVIRAKDLPPGLVETGMAPVTRPGPADGWRTDDEALRERVRQFRGTRRDLAQALG
HHHCCCEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHC
MSERTLYRRLRDLGIR
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2836706; 2710108 [H]