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The map label for this gene is pobB [H]

Identifier: 73537414

GI number: 73537414

Start: 135407

End: 136363

Strand: Direct

Name: pobB [H]

Synonym: Reut_B3579

Alternate gene names: 73537414

Gene position: 135407-136363 (Clockwise)

Preceding gene: 73537413

Following gene: 73537415

Centisome position: 4.97

GC content: 68.03

Gene sequence:

>957_bases
ATGAGTGCCCACAAGACCCTGACGATGCGAGTCCAGGCCATGCGCTACGAAGCGCGCGGCATTGTCAGCCTCGAGCTGCA
AGATCCCGACGGGGCCACGCTGCCCGAATACGGTCCGGGCGCCCATATCGACCTGCACCTCGGCAATGGCCTGGTCCGCA
GCTACTCGCTGTGCGGCGCGCCGGAAGTCCGCGACCGCTACACCGTGGGTGTCCTGCAGGACCGCAACAGCCGCGGCGGC
TCGCGCTACGTGCATGAGCAACTGCGCGTCGGCGCCACGCTGAACGTCAGCGCGCCGCGCAACAACTTCGAACTCGATGA
AAGCGCCGCGCACACCGTGCTCGTCGCCGGCGGCATCGGCGTCACGCCGATCGTCTGCATGGCACGCCGCCTGGCCGAGC
TGGGCCGTTCGTTCACGCTGCTGTACTGCGCCCGCACGCGCGCCGAAGCGGCGTTCGTCGATTCGCTGTCGGCGTATGGC
GACGCGGTGCGTTTCCACTTCGACGACGAAGCCGGTGCTCCGCCCGACATGAAGGCCATGCTGGCCGGCCAGGACGCGCA
GACGCACCTGTACTGCTGCGGCCCCGGCCCGATGCTCAATGCGTTCGAAGCCGCGTGCGAGGCGCACGGCTATCCGAACG
TCCACATCGAACGCTTTGCCGCCGATCCGTCGACCGAGTCGGTGCAGGAAGGCGAATACGTCGTGCAGCTGTCGCGCACG
GGCTCCCTCGTCAAGGTGCCGTCGGGCAAGTCGCTGCTCGACGCACTGCTCGAAGCCGGCCTGGACGTCGAGCACAGCTG
CCGCGAAGGCGTGTGCGGTTCGTGCGAGACCGCTGTGCTGGAAGGCTGCCCCGACCACCGCGACAGCGTGCTCAGCAACA
GCGAGCGCGCCTCCAACAAGACCATGATGGTCTGCGTGTCCGGCTGCAAGGGCAGCCGGCTGGTGCTGGACCTGTAA

Upstream 100 bases:

>100_bases
GACCGAGAAGCGCTGAACTCGCTGAACGCGAGCGCAAGACCAGGCTAGTAGCGCCGCGCTGTATTGTGCGCGGCGCGCCA
AGGAAACAGAAGGAAGACAC

Downstream 100 bases:

>100_bases
GTCCACGCCCGACGGCGAGGCCTGCCAGCCTCGCCGGCGCTTCACCGTGCGCCCGAGCGCGCAACCGAAGCCCTCCGTGC
CGCGGCGCGATGGCTGACTG

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NA

Alternate protein names: 4-carboxydiphenyl ether; phenoxybenzoate dioxygenase subunit beta [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSAHKTLTMRVQAMRYEARGIVSLELQDPDGATLPEYGPGAHIDLHLGNGLVRSYSLCGAPEVRDRYTVGVLQDRNSRGG
SRYVHEQLRVGATLNVSAPRNNFELDESAAHTVLVAGGIGVTPIVCMARRLAELGRSFTLLYCARTRAEAAFVDSLSAYG
DAVRFHFDDEAGAPPDMKAMLAGQDAQTHLYCCGPGPMLNAFEAACEAHGYPNVHIERFAADPSTESVQEGEYVVQLSRT
GSLVKVPSGKSLLDALLEAGLDVEHSCREGVCGSCETAVLEGCPDHRDSVLSNSERASNKTMMVCVSGCKGSRLVLDL

Sequences:

>Translated_318_residues
MSAHKTLTMRVQAMRYEARGIVSLELQDPDGATLPEYGPGAHIDLHLGNGLVRSYSLCGAPEVRDRYTVGVLQDRNSRGG
SRYVHEQLRVGATLNVSAPRNNFELDESAAHTVLVAGGIGVTPIVCMARRLAELGRSFTLLYCARTRAEAAFVDSLSAYG
DAVRFHFDDEAGAPPDMKAMLAGQDAQTHLYCCGPGPMLNAFEAACEAHGYPNVHIERFAADPSTESVQEGEYVVQLSRT
GSLVKVPSGKSLLDALLEAGLDVEHSCREGVCGSCETAVLEGCPDHRDSVLSNSERASNKTMMVCVSGCKGSRLVLDL
>Mature_317_residues
SAHKTLTMRVQAMRYEARGIVSLELQDPDGATLPEYGPGAHIDLHLGNGLVRSYSLCGAPEVRDRYTVGVLQDRNSRGGS
RYVHEQLRVGATLNVSAPRNNFELDESAAHTVLVAGGIGVTPIVCMARRLAELGRSFTLLYCARTRAEAAFVDSLSAYGD
AVRFHFDDEAGAPPDMKAMLAGQDAQTHLYCCGPGPMLNAFEAACEAHGYPNVHIERFAADPSTESVQEGEYVVQLSRTG
SLVKVPSGKSLLDALLEAGLDVEHSCREGVCGSCETAVLEGCPDHRDSVLSNSERASNKTMMVCVSGCKGSRLVLDL

Specific function: Degrades exclusively diarylether compounds having carboxyl groups in the 3- or 4-position. Yields a hemiacetal that spontaneously hydrolyzes to phenol and protocatechuate [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=325, Percent_Identity=35.0769230769231, Blast_Score=191, Evalue=4e-50,
Organism=Escherichia coli, GI1787658, Length=323, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787098, Length=238, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 34089; Mature: 33958

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHKTLTMRVQAMRYEARGIVSLELQDPDGATLPEYGPGAHIDLHLGNGLVRSYSLCGA
CCCCCEEEEEEEEEEHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCC
PEVRDRYTVGVLQDRNSRGGSRYVHEQLRVGATLNVSAPRNNFELDESAAHTVLVAGGIG
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCC
VTPIVCMARRLAELGRSFTLLYCARTRAEAAFVDSLSAYGDAVRFHFDDEAGAPPDMKAM
HHHHHHHHHHHHHHCCCEEEEEEEHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHE
LAGQDAQTHLYCCGPGPMLNAFEAACEAHGYPNVHIERFAADPSTESVQEGEYVVQLSRT
ECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECC
GSLVKVPSGKSLLDALLEAGLDVEHSCREGVCGSCETAVLEGCPDHRDSVLSNSERASNK
CCEEECCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCC
TMMVCVSGCKGSRLVLDL
EEEEEEECCCCCEEEEEC
>Mature Secondary Structure 
SAHKTLTMRVQAMRYEARGIVSLELQDPDGATLPEYGPGAHIDLHLGNGLVRSYSLCGA
CCCCEEEEEEEEEEHHCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCC
PEVRDRYTVGVLQDRNSRGGSRYVHEQLRVGATLNVSAPRNNFELDESAAHTVLVAGGIG
CCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCEEEEECCCC
VTPIVCMARRLAELGRSFTLLYCARTRAEAAFVDSLSAYGDAVRFHFDDEAGAPPDMKAM
HHHHHHHHHHHHHHCCCEEEEEEEHHCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHE
LAGQDAQTHLYCCGPGPMLNAFEAACEAHGYPNVHIERFAADPSTESVQEGEYVVQLSRT
ECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEEECC
GSLVKVPSGKSLLDALLEAGLDVEHSCREGVCGSCETAVLEGCPDHRDSVLSNSERASNK
CCEEECCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHCCCCCHHHHHHCCCCCCCCC
TMMVCVSGCKGSRLVLDL
EEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7710319 [H]