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The map label for this gene is nsrR [H]

Identifier: 73542904

GI number: 73542904

Start: 3532603

End: 3533055

Strand: Reverse

Name: nsrR [H]

Synonym: Reut_A3220

Alternate gene names: 73542904

Gene position: 3533055-3532603 (Counterclockwise)

Preceding gene: 73542916

Following gene: 73542903

Centisome position: 92.82

GC content: 65.34

Gene sequence:

>453_bases
ATGCAACTGACCCGCTTTTCCGACTACGCACTGCGGCTGCTGATGTACGTGGCACGCGCCGACGGCAGCCGGCCTATCAC
CATTGCCGAGGTGGGCCAGCAGTTCGAGGTTTCCCATCACCATCTGGTGAAGGTGGCAGCGCGCCTGTCGAAGCTGGGCT
GGATCACGGCCACGCGCGGCCGCCACGGGGGCCTGGAACTGGGGCCGCAGGCTGACCAGCTGACCATCGGGACGATCCTG
CGCGAACTGGAAGGGCACAAGCCGGTCATCGACTGTGATGACCCGCCATGTGCCCTGAAGGGAAACTGCCGGCTGCGCCG
GGCGCTGGACGAGGCGGAGCAGGCGTTCTATCGCTCGCTTGATACTGTCACCCTGGCGGATGTCACCGGCAGCCAGACTG
CCGAGTCGATCATCCGGCTGCATCGCGATTTTGTGAGCCGGCGTACGGCTTGA

Upstream 100 bases:

>100_bases
GAGGAATTCTCGCGGCCCGGAGTCTGATTTGTGTCACGGGATTCCGCGCCGCACAATAAAACTTGCATTTGAAATGCCGC
TTATCTAAGATGTCACCACG

Downstream 100 bases:

>100_bases
ACGGCGCGCTTCGAGCCGCAGTTCTGGCGTTACGTGCGCCGGCTTTGTTGTGAGTAAAAACATGCATTTTGTTTGCATGA
TTTAAAACGGAGCCTTACCT

Product: BadM/Rrf2 family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MQLTRFSDYALRLLMYVARADGSRPITIAEVGQQFEVSHHHLVKVAARLSKLGWITATRGRHGGLELGPQADQLTIGTIL
RELEGHKPVIDCDDPPCALKGNCRLRRALDEAEQAFYRSLDTVTLADVTGSQTAESIIRLHRDFVSRRTA

Sequences:

>Translated_150_residues
MQLTRFSDYALRLLMYVARADGSRPITIAEVGQQFEVSHHHLVKVAARLSKLGWITATRGRHGGLELGPQADQLTIGTIL
RELEGHKPVIDCDDPPCALKGNCRLRRALDEAEQAFYRSLDTVTLADVTGSQTAESIIRLHRDFVSRRTA
>Mature_150_residues
MQLTRFSDYALRLLMYVARADGSRPITIAEVGQQFEVSHHHLVKVAARLSKLGWITATRGRHGGLELGPQADQLTIGTIL
RELEGHKPVIDCDDPPCALKGNCRLRRALDEAEQAFYRSLDTVTLADVTGSQTAESIIRLHRDFVSRRTA

Specific function: Binds DNA; this binding is disrupted by nitrosylation upon exposure to nitric oxide (NO) and also by EDTA and iron chelators. The 2Fe-2S cluster is stable in the presence of O(2) [H]

COG id: COG1959

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH rrf2-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790621, Length=129, Percent_Identity=42.6356589147287, Blast_Score=111, Evalue=2e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000944
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02082 Rrf2 [H]

EC number: NA

Molecular weight: Translated: 16706; Mature: 16706

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTRFSDYALRLLMYVARADGSRPITIAEVGQQFEVSHHHLVKVAARLSKLGWITATRG
CCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHCCEEECCC
RHGGLELGPQADQLTIGTILRELEGHKPVIDCDDPPCALKGNCRLRRALDEAEQAFYRSL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DTVTLADVTGSQTAESIIRLHRDFVSRRTA
CCEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQLTRFSDYALRLLMYVARADGSRPITIAEVGQQFEVSHHHLVKVAARLSKLGWITATRG
CCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHCCEEECCC
RHGGLELGPQADQLTIGTILRELEGHKPVIDCDDPPCALKGNCRLRRALDEAEQAFYRSL
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DTVTLADVTGSQTAESIIRLHRDFVSRRTA
CCEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]