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The map label for this gene is hmp [H]

Identifier: 73542903

GI number: 73542903

Start: 3531281

End: 3532501

Strand: Reverse

Name: hmp [H]

Synonym: Reut_A3219

Alternate gene names: 73542903

Gene position: 3532501-3531281 (Counterclockwise)

Preceding gene: 73542904

Following gene: 73542902

Centisome position: 92.8

GC content: 67.9

Gene sequence:

>1221_bases
ATGTTGTCTGCTGCTTCTCGCCCCTACATTGATGCCAGCGTACCGGTGCTGCGTGAACATGGCCTCGCCATCACTACCCA
TTTCTACCGCGAGATGTTCGCGGCGCGCCCTGAACTGACCCAACTGTTCAACATGGGCAACCAGGCCAACGGCTCGCAGC
AGCAGTCGCTCGCCTCCGCCGTGTTCGCCTACGCCGCCAATATCGACAACGCCAACGCGCTTGCGCCGGTGGTCGAACGC
ATCGTGCACAAGCATGCAGCGGTGGGCCTGAAGCCCGCGCATTACCCGATCGTCGGCCGGCACCTGCTGGGCGCCATCTC
CGCCGTGCTCGGCGAAGCCGCCACGCCCGATCTGATTGCCGCCTGGGACGAAGCGTACTGGCTGCTGGCCGGCGAGTTGA
TCGCCGCCGAAGCGCGCCTGTACCAGAGCACCGGCATGGCCGCCGGCGAACGCATCGCCGTACGGGTCGACCGCCGCGAA
GTGCAGAGCGACACCGTGGTCGCGCTGACGCTGTCGGCCGTGGACGGCCAGCCGCTGCGCGATTTCCGCCCGGGCCAGTA
CGTCAGCGTCGAAGTCACGCTCGATGACGGCAACCGCCAGCAGCGCCAGTACTCGCTGTCTGCGGAGCGCGGCCTGCCGA
CGTGGCAAATCTCGGTCAAGCGCGAGGACGGCGACCACGCCACGCCGGCCGGCGCAGTATCCAACTGGCTGCATGCCAAC
GCGCAGCCCGGCACGGAACTGTCGGTGAGCGCGCCGTTCGGCGACTTCGCGCCGCGCCTGGATAACCACCGCCCGATCGT
GCTGCTGTCGGCCGGTATCGGCATCACGCCGATGCTGTCGGTGCTGCGCACGTTGGCCGCACAAGGCTCCAGGCGCGAGA
TTCTGTTTGCCCACGCGGCCCGCGACGGCCGCCACCACGCCCATCGTGCCGACGTGGCCTGGGCCAGGGAACGCCTGCCG
CAGCTGCGTACCCATATCAGCTACGAACAGCCGCAAGCCGCCGATGTCGCGGGCCGTGACTACGACCACGCGGGCACCAT
GCCCGTCGCCGCACTGCTCGATGCGCCCGACAACCGTCTGTTTATCGACGGCGACTTCCACCTGTGCGGACCGCTCGGGT
TCATGCAGGCGCAGCGGCACGCGCTGATCAGCGCTGGTGTCCCGGTCGGGCATATCCACCGCGAAGTGTTCGGCCCGGAT
CTGCTCGACGACCTGTTGTAA

Upstream 100 bases:

>100_bases
CGGCGCGCTTCGAGCCGCAGTTCTGGCGTTACGTGCGCCGGCTTTGTTGTGAGTAAAAACATGCATTTTGTTTGCATGAT
TTAAAACGGAGCCTTACCTG

Downstream 100 bases:

>100_bases
GCCGCCACAGACACGGTCATAGGGCTCTGATACGCTTGGCGCATTCCATGTCGAGCGTATCCCTGATTCCGTGCAACTCT
CCATCCGGCCCGCCCTCCGT

Product: globin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELTQLFNMGNQANGSQQQSLASAVFAYAANIDNANALAPVVER
IVHKHAAVGLKPAHYPIVGRHLLGAISAVLGEAATPDLIAAWDEAYWLLAGELIAAEARLYQSTGMAAGERIAVRVDRRE
VQSDTVVALTLSAVDGQPLRDFRPGQYVSVEVTLDDGNRQQRQYSLSAERGLPTWQISVKREDGDHATPAGAVSNWLHAN
AQPGTELSVSAPFGDFAPRLDNHRPIVLLSAGIGITPMLSVLRTLAAQGSRREILFAHAARDGRHHAHRADVAWARERLP
QLRTHISYEQPQAADVAGRDYDHAGTMPVAALLDAPDNRLFIDGDFHLCGPLGFMQAQRHALISAGVPVGHIHREVFGPD
LLDDLL

Sequences:

>Translated_406_residues
MLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELTQLFNMGNQANGSQQQSLASAVFAYAANIDNANALAPVVER
IVHKHAAVGLKPAHYPIVGRHLLGAISAVLGEAATPDLIAAWDEAYWLLAGELIAAEARLYQSTGMAAGERIAVRVDRRE
VQSDTVVALTLSAVDGQPLRDFRPGQYVSVEVTLDDGNRQQRQYSLSAERGLPTWQISVKREDGDHATPAGAVSNWLHAN
AQPGTELSVSAPFGDFAPRLDNHRPIVLLSAGIGITPMLSVLRTLAAQGSRREILFAHAARDGRHHAHRADVAWARERLP
QLRTHISYEQPQAADVAGRDYDHAGTMPVAALLDAPDNRLFIDGDFHLCGPLGFMQAQRHALISAGVPVGHIHREVFGPD
LLDDLL
>Mature_406_residues
MLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELTQLFNMGNQANGSQQQSLASAVFAYAANIDNANALAPVVER
IVHKHAAVGLKPAHYPIVGRHLLGAISAVLGEAATPDLIAAWDEAYWLLAGELIAAEARLYQSTGMAAGERIAVRVDRRE
VQSDTVVALTLSAVDGQPLRDFRPGQYVSVEVTLDDGNRQQRQYSLSAERGLPTWQISVKREDGDHATPAGAVSNWLHAN
AQPGTELSVSAPFGDFAPRLDNHRPIVLLSAGIGITPMLSVLRTLAAQGSRREILFAHAARDGRHHAHRADVAWARERLP
QLRTHISYEQPQAADVAGRDYDHAGTMPVAALLDAPDNRLFIDGDFHLCGPLGFMQAQRHALISAGVPVGHIHREVFGPD
LLDDLL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=405, Percent_Identity=39.2592592592593, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1787658, Length=246, Percent_Identity=27.6422764227642, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=414, Percent_Identity=34.0579710144928, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44053; Mature: 44053

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELTQLFNMGNQANGSQQQSLASA
CCCCCCCCCCCCCCCHHHHCCCEEHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
VFAYAANIDNANALAPVVERIVHKHAAVGLKPAHYPIVGRHLLGAISAVLGEAATPDLIA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
AWDEAYWLLAGELIAAEARLYQSTGMAAGERIAVRVDRREVQSDTVVALTLSAVDGQPLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCEEEEEEEEECCCCCCC
DFRPGQYVSVEVTLDDGNRQQRQYSLSAERGLPTWQISVKREDGDHATPAGAVSNWLHAN
CCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCC
AQPGTELSVSAPFGDFAPRLDNHRPIVLLSAGIGITPMLSVLRTLAAQGSRREILFAHAA
CCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECC
RDGRHHAHRADVAWARERLPQLRTHISYEQPQAADVAGRDYDHAGTMPVAALLDAPDNRL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCEE
FIDGDFHLCGPLGFMQAQRHALISAGVPVGHIHREVFGPDLLDDLL
EEECCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELTQLFNMGNQANGSQQQSLASA
CCCCCCCCCCCCCCCHHHHCCCEEHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
VFAYAANIDNANALAPVVERIVHKHAAVGLKPAHYPIVGRHLLGAISAVLGEAATPDLIA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
AWDEAYWLLAGELIAAEARLYQSTGMAAGERIAVRVDRREVQSDTVVALTLSAVDGQPLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCEEEEEEEEECCCCCCC
DFRPGQYVSVEVTLDDGNRQQRQYSLSAERGLPTWQISVKREDGDHATPAGAVSNWLHAN
CCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHCCC
AQPGTELSVSAPFGDFAPRLDNHRPIVLLSAGIGITPMLSVLRTLAAQGSRREILFAHAA
CCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECC
RDGRHHAHRADVAWARERLPQLRTHISYEQPQAADVAGRDYDHAGTMPVAALLDAPDNRL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCEE
FIDGDFHLCGPLGFMQAQRHALISAGVPVGHIHREVFGPDLLDDLL
EEECCCEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14521231; 11481432 [H]