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The map label for this gene is b2999 [C]

Identifier: 73542864

GI number: 73542864

Start: 3479599

End: 3480900

Strand: Reverse

Name: b2999 [C]

Synonym: Reut_A3180

Alternate gene names: 73542864

Gene position: 3480900-3479599 (Counterclockwise)

Preceding gene: 73542865

Following gene: 73542863

Centisome position: 91.45

GC content: 64.29

Gene sequence:

>1302_bases
ATGCAGGCAGCAGCGGAAGCGCTCGCTGCGGCAGTCCCCACAGAACCTATGGAACTTAAAGGAGCGACAGGCATGGGCCA
GACTATCCAGATCCAGACCCAGGACGGCAGTTTCAGCGGTTATCTCGCCATCCCGGCGGCAGGCAAGGGCCCCGGCATCG
TGTTATGCCAGGAGATCTTCGGGGTCAATGCCACGATGCGGCAAGTAGCGGACTACTACGCCGAGGAAGGCTACACGGTG
CTGGTGCCGGACTTGTTCTGGCGTATTGCGCCCGGAATCGAGCTGACGGACCGAGGCGAGGACTTCCAGCGGGCGCTAGG
GCTATATCAGCAGTTCGACGAGGACAAGGGCGTGCAGGATGTGGGCGCCGCGCTCGATGCACTGCGCGCACGGCCAGAAT
GCGTCGGCCAGACCGGTGTGCTTGGCTTCTGCCTGGGTGGCAAGCTGGCCTACCTGGCGGCGTGCCGGTTGCCCGGCGTG
GCTTGTGCCGTCGGCTACTATGGCGTCGGCATCGAACGGGCGCTGGACGAGGCGGACAATATCCGAGGACGGCTCGTGCT
GCATATTGCCGAACGAGATGGCTTTTGCTCACCCGAAGCGCAGGCGCAGATTCGTGAAGCGCTGGGCGGCCGGCCCAGCA
TCGAACTGTACGTCTATCCTGGCATGGACCACGCCTTCGCGCGCGTTGGCGGCGAGCACTTCGACAAGGCGTCGGCACTG
ATGGCGCACCAGCGCTCCATCGCGGCGTACCGCAGCGAGATGGGCCCGCACTACGACTTCTCCGCGCTATGGGACAAGCA
CTGCGAGCACGAATTCGCCACGCGCGATGTGGATGCCACCATGGCAACGATGGTGCCCGAGCCCTACGTCAACCACATCC
CGACGATGACCGGAGGCGTGGGCTACCGCCAGCTCAAGCGCTTCTACCAGCACCACTTCGTCCACAGCAATCCGCCTGAC
ACCACGCTGATCCCGCTGTCGCGCACGATCGGCGCGAGCCAGGTGGTCGACGAGATGCTGTTCTGCTTCACCCATACTTG
CGAGATCGACTGGATGCTGCCCGGCGTGCCGCCGACCGGCAAGCGCGTGGAGATCCCGCTGATCGCGATTGTCAAGTTCC
GCGGGGACAAGCTCTACCACGAGCACATCTACTGGGATCAGGCCAGCGTGCTCGTGCAGATCGGCAAGCTCGACCCCAAG
GGTTTGCCGGTGGCAGGCGTGGAGACCGCGCGCAAGCTGCTGGAAGAAACACAGCCGTCTAACACGCTGATGGCGCGGTG
GAGCGAGAGCGAGCGCCAGTGA

Upstream 100 bases:

>100_bases
TGTGCCGATTTGCCTTTCCAGTACTGGCTCAATCTCTGCGTCAGACACTCCCCCGATATTCCGCACTGAGCCGTCCATGT
CGCGGTGCGGGTCTGCCATC

Downstream 100 bases:

>100_bases
ACGGGGCATGACTTTGAACGCTTCGGCGGCCATCGCGCCGCCGCAAGTCGGCTTCGCGCCGCAATTTCACGGAATCCGAC
GATCGCCGCAGGAAGTCCCC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MQAAAEALAAAVPTEPMELKGATGMGQTIQIQTQDGSFSGYLAIPAAGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTV
LVPDLFWRIAPGIELTDRGEDFQRALGLYQQFDEDKGVQDVGAALDALRARPECVGQTGVLGFCLGGKLAYLAACRLPGV
ACAVGYYGVGIERALDEADNIRGRLVLHIAERDGFCSPEAQAQIREALGGRPSIELYVYPGMDHAFARVGGEHFDKASAL
MAHQRSIAAYRSEMGPHYDFSALWDKHCEHEFATRDVDATMATMVPEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPD
TTLIPLSRTIGASQVVDEMLFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIVKFRGDKLYHEHIYWDQASVLVQIGKLDPK
GLPVAGVETARKLLEETQPSNTLMARWSESERQ

Sequences:

>Translated_433_residues
MQAAAEALAAAVPTEPMELKGATGMGQTIQIQTQDGSFSGYLAIPAAGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTV
LVPDLFWRIAPGIELTDRGEDFQRALGLYQQFDEDKGVQDVGAALDALRARPECVGQTGVLGFCLGGKLAYLAACRLPGV
ACAVGYYGVGIERALDEADNIRGRLVLHIAERDGFCSPEAQAQIREALGGRPSIELYVYPGMDHAFARVGGEHFDKASAL
MAHQRSIAAYRSEMGPHYDFSALWDKHCEHEFATRDVDATMATMVPEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPD
TTLIPLSRTIGASQVVDEMLFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIVKFRGDKLYHEHIYWDQASVLVQIGKLDPK
GLPVAGVETARKLLEETQPSNTLMARWSESERQ
>Mature_433_residues
MQAAAEALAAAVPTEPMELKGATGMGQTIQIQTQDGSFSGYLAIPAAGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTV
LVPDLFWRIAPGIELTDRGEDFQRALGLYQQFDEDKGVQDVGAALDALRARPECVGQTGVLGFCLGGKLAYLAACRLPGV
ACAVGYYGVGIERALDEADNIRGRLVLHIAERDGFCSPEAQAQIREALGGRPSIELYVYPGMDHAFARVGGEHFDKASAL
MAHQRSIAAYRSEMGPHYDFSALWDKHCEHEFATRDVDATMATMVPEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPD
TTLIPLSRTIGASQVVDEMLFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIVKFRGDKLYHEHIYWDQASVLVQIGKLDPK
GLPVAGVETARKLLEETQPSNTLMARWSESERQ

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=258, Percent_Identity=23.2558139534884, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 47532; Mature: 47532

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAAAEALAAAVPTEPMELKGATGMGQTIQIQTQDGSFSGYLAIPAAGKGPGIVLCQEIF
CCHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEHHHH
GVNATMRQVADYYAEEGYTVLVPDLFWRIAPGIELTDRGEDFQRALGLYQQFDEDKGVQD
CCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCCHHH
VGAALDALRARPECVGQTGVLGFCLGGKLAYLAACRLPGVACAVGYYGVGIERALDEADN
HHHHHHHHHCCHHHCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHC
IRGRLVLHIAERDGFCSPEAQAQIREALGGRPSIELYVYPGMDHAFARVGGEHFDKASAL
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHH
MAHQRSIAAYRSEMGPHYDFSALWDKHCEHEFATRDVDATMATMVPEPYVNHIPTMTGGV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCC
GYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEMLFCFTHTCEIDWMLPGVPPTG
CHHHHHHHHHHHCCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
KRVEIPLIAIVKFRGDKLYHEHIYWDQASVLVQIGKLDPKGLPVAGVETARKLLEETQPS
CEEEEEEEEEEEECCCEEEHHHEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
NTLMARWSESERQ
CCEEEECCCCCCC
>Mature Secondary Structure
MQAAAEALAAAVPTEPMELKGATGMGQTIQIQTQDGSFSGYLAIPAAGKGPGIVLCQEIF
CCHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEEEHHHH
GVNATMRQVADYYAEEGYTVLVPDLFWRIAPGIELTDRGEDFQRALGLYQQFDEDKGVQD
CCCHHHHHHHHHHHHCCCEEEECHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCCHHH
VGAALDALRARPECVGQTGVLGFCLGGKLAYLAACRLPGVACAVGYYGVGIERALDEADN
HHHHHHHHHCCHHHCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHC
IRGRLVLHIAERDGFCSPEAQAQIREALGGRPSIELYVYPGMDHAFARVGGEHFDKASAL
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHH
MAHQRSIAAYRSEMGPHYDFSALWDKHCEHEFATRDVDATMATMVPEPYVNHIPTMTGGV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHCCHHHHHHHHCCCHHHHHCCCCCCCC
GYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEMLFCFTHTCEIDWMLPGVPPTG
CHHHHHHHHHHHCCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHCCEEEEEECCCCCCC
KRVEIPLIAIVKFRGDKLYHEHIYWDQASVLVQIGKLDPKGLPVAGVETARKLLEETQPS
CEEEEEEEEEEEECCCEEEHHHEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCC
NTLMARWSESERQ
CCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8138139 [H]