Click here to switch to the map view.

The map label for this gene is mutT [H]

Identifier: 73542651

GI number: 73542651

Start: 3253334

End: 3253762

Strand: Reverse

Name: mutT [H]

Synonym: Reut_A2967

Alternate gene names: 73542651

Gene position: 3253762-3253334 (Counterclockwise)

Preceding gene: 73542652

Following gene: 73542650

Centisome position: 85.48

GC content: 67.37

Gene sequence:

>429_bases
ATGACGCAGACGCAAGACAGCACGACGCCCCGCAAGGTGACCGAAGTGGCCGTGGGCGTGCTGGTGCAGCCCGATGGCCG
CTTCCTGCTGGCGCAGCGCCCGGGCGGCAAGCCGTATGAGGGCTACTGGGAATTTCCCGGCGGCAAGCTGGAACCGGGCG
AGACGGTGGAAGCCGCGCTCGCGCGCGAGTTGCATGAAGAGCTGGGCCTGGACATTACCGAGTGCGTGCGCTGGCATGTG
CTAGAATACGATTACCCGCACGCCTACGTGCGGCTGCATTTCTGCAAGGTCACGGCCTGGCGCGGTGACCCGGTCGGCCG
CGAAGGGCAGGCTTTTTCCTGGCAGACGGTCCCCGTGACGGTGGAACCGCTGCTGCCGGCCACCATCCCGGTGGTCGAAT
GGCTGGGCGAAGAATCCCGGCCCGCCTGA

Upstream 100 bases:

>100_bases
CGCTCGAACGCGGCTCGCGTTCGGGCCGCGTGGCGTGGCAGTTTGCGCGCGACTGGGGCGGCAAGCATGGCAAGCCGTTC
ATCGCGGATGTGGAGGGCGC

Downstream 100 bases:

>100_bases
CAATTCGAGCTTTACCCGCGCCCCTGCGGCTGCGGCGCCCGTGATCGCGCCGGCATAACAACAGCAAGAAAGGATCCGTA
TGCTACGTGCAGACCGCGTT

Product: NUDIX hydrolase

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MTQTQDSTTPRKVTEVAVGVLVQPDGRFLLAQRPGGKPYEGYWEFPGGKLEPGETVEAALARELHEELGLDITECVRWHV
LEYDYPHAYVRLHFCKVTAWRGDPVGREGQAFSWQTVPVTVEPLLPATIPVVEWLGEESRPA

Sequences:

>Translated_142_residues
MTQTQDSTTPRKVTEVAVGVLVQPDGRFLLAQRPGGKPYEGYWEFPGGKLEPGETVEAALARELHEELGLDITECVRWHV
LEYDYPHAYVRLHFCKVTAWRGDPVGREGQAFSWQTVPVTVEPLLPATIPVVEWLGEESRPA
>Mature_141_residues
TQTQDSTTPRKVTEVAVGVLVQPDGRFLLAQRPGGKPYEGYWEFPGGKLEPGETVEAALARELHEELGLDITECVRWHVL
EYDYPHAYVRLHFCKVTAWRGDPVGREGQAFSWQTVPVTVEPLLPATIPVVEWLGEESRPA

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=122, Percent_Identity=35.2459016393443, Blast_Score=80, Evalue=6e-17,
Organism=Escherichia coli, GI1788056, Length=125, Percent_Identity=40, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 15940; Mature: 15809

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTQDSTTPRKVTEVAVGVLVQPDGRFLLAQRPGGKPYEGYWEFPGGKLEPGETVEAAL
CCCCCCCCCCHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHH
ARELHEELGLDITECVRWHVLEYDYPHAYVRLHFCKVTAWRGDPVGREGQAFSWQTVPVT
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEECCEE
VEPLLPATIPVVEWLGEESRPA
ECCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQTQDSTTPRKVTEVAVGVLVQPDGRFLLAQRPGGKPYEGYWEFPGGKLEPGETVEAAL
CCCCCCCCCHHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCHHHHHHH
ARELHEELGLDITECVRWHVLEYDYPHAYVRLHFCKVTAWRGDPVGREGQAFSWQTVPVT
HHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCEEEEECCEE
VEPLLPATIPVVEWLGEESRPA
ECCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8244038 [H]