Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 73542598

GI number: 73542598

Start: 3189137

End: 3190078

Strand: Reverse

Name: gcvA [H]

Synonym: Reut_A2914

Alternate gene names: 73542598

Gene position: 3190078-3189137 (Counterclockwise)

Preceding gene: 73542603

Following gene: 73542596

Centisome position: 83.81

GC content: 63.16

Gene sequence:

>942_bases
ATGCAGATCGATTCCTCCATCCTCGGGGCGCTGCGGTGTTTCGAAGCCGCAGGGCGCCTGCTGAGCTTCACCAAGGCCGC
CGAATCGCTGAACCTCACCCAAAGCGCGGTGAGCCAGCAGATTCGCCATCTGGAAGACCGTCTCGGCTACGTGCTTTTCG
TGCGCCAGCCGCGCAGCCTGAAACTGACGCCACAGGGCGAGGTGCTGTTCGAGACCACGACGCGCGCACTGCATGACATC
CAGCAGACCATCCAGCGACTCGGCATGCCGAACGCGCCGCTGCAGGTCAACTGCCTGCCGTCGTTCGCGCTGCAATGGCT
GATGCCGCGCCTGACCGATTTCCACCGCGAACAGCCGGACGTTTCCGTACGCTTGAAAGCCGAGTTCCAGGCTTTGGACA
GGCAATCGATGGACACGGAAGACATCGACGTGGCCGTGCGTTACGACCCGGTCCGCTACAGCCGGCTGCACGCGGATGTC
TTGCTGGACGAGTATCTGATTCCGGTGGCTACGCCCGAGTATCTCGCCGAGCATCGCGAGCTAGCCGAGGGGACGTCGCT
CGACGGCGTCGTGTTGCTGCACGACGCATCGCCATGGGTCGGTGCGGCCGAGTTCGTGGAATGGCGCACATGGCTTGAGG
CGTTTCATCCGACATGGATGGCGCATCTGGAAGGGCCGCAATTCAATCTGTCGAGCCTGGCCATCAGCGCGGCGCTGAAC
CACCAGGGCGTGGCGATGGGACGTACGGCGCTGGTTTATGACGAGATCAGGAGCGGGCGACTGGTGAATGTGTTCCGCCA
GCACGTACGCGCGCCGGCGCGCTACATGCTGCTTTCCCGGAATCCGGATGATCGGCGTGTGACCATTTTCTCGGCATGGC
TCAAGGCAGAGTGCGCGCGGTTTGATGCGATGCGGATGGAGACGCTTTCGCGGGATCGCTAG

Upstream 100 bases:

>100_bases
TCATTCTCCAGAAAAGTCCTTACTGAAAGCGATCGTAAGGGCCGCAGCCGTGCAAGAATAGCGATCGTTTCTAACGCATG
CATTAGTCAGGCTAATAGCC

Downstream 100 bases:

>100_bases
GCGCCGCTCGCGGCGCTTCGATCGTCTGACTGTGCTGCTGCGTGGCGTCGGCTGGCCGGTCCGCGTGCGCCACAAGCCGA
CCGGCAGCCGCCGCGAACCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MQIDSSILGALRCFEAAGRLLSFTKAAESLNLTQSAVSQQIRHLEDRLGYVLFVRQPRSLKLTPQGEVLFETTTRALHDI
QQTIQRLGMPNAPLQVNCLPSFALQWLMPRLTDFHREQPDVSVRLKAEFQALDRQSMDTEDIDVAVRYDPVRYSRLHADV
LLDEYLIPVATPEYLAEHRELAEGTSLDGVVLLHDASPWVGAAEFVEWRTWLEAFHPTWMAHLEGPQFNLSSLAISAALN
HQGVAMGRTALVYDEIRSGRLVNVFRQHVRAPARYMLLSRNPDDRRVTIFSAWLKAECARFDAMRMETLSRDR

Sequences:

>Translated_313_residues
MQIDSSILGALRCFEAAGRLLSFTKAAESLNLTQSAVSQQIRHLEDRLGYVLFVRQPRSLKLTPQGEVLFETTTRALHDI
QQTIQRLGMPNAPLQVNCLPSFALQWLMPRLTDFHREQPDVSVRLKAEFQALDRQSMDTEDIDVAVRYDPVRYSRLHADV
LLDEYLIPVATPEYLAEHRELAEGTSLDGVVLLHDASPWVGAAEFVEWRTWLEAFHPTWMAHLEGPQFNLSSLAISAALN
HQGVAMGRTALVYDEIRSGRLVNVFRQHVRAPARYMLLSRNPDDRRVTIFSAWLKAECARFDAMRMETLSRDR
>Mature_313_residues
MQIDSSILGALRCFEAAGRLLSFTKAAESLNLTQSAVSQQIRHLEDRLGYVLFVRQPRSLKLTPQGEVLFETTTRALHDI
QQTIQRLGMPNAPLQVNCLPSFALQWLMPRLTDFHREQPDVSVRLKAEFQALDRQSMDTEDIDVAVRYDPVRYSRLHADV
LLDEYLIPVATPEYLAEHRELAEGTSLDGVVLLHDASPWVGAAEFVEWRTWLEAFHPTWMAHLEGPQFNLSSLAISAALN
HQGVAMGRTALVYDEIRSGRLVNVFRQHVRAPARYMLLSRNPDDRRVTIFSAWLKAECARFDAMRMETLSRDR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=36.026936026936, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=32.7702702702703, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786448, Length=256, Percent_Identity=35.546875, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI157672245, Length=232, Percent_Identity=30.6034482758621, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790262, Length=167, Percent_Identity=31.1377245508982, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788887, Length=259, Percent_Identity=25.8687258687259, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=253, Percent_Identity=25.296442687747, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=174, Percent_Identity=32.183908045977, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=23.9202657807309, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081978, Length=270, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787589, Length=141, Percent_Identity=31.2056737588652, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=143, Percent_Identity=29.3706293706294, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789639, Length=161, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081904, Length=170, Percent_Identity=28.2352941176471, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35724; Mature: 35724

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50006 FHA_DOMAIN ; PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIDSSILGALRCFEAAGRLLSFTKAAESLNLTQSAVSQQIRHLEDRLGYVLFVRQPRSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
KLTPQGEVLFETTTRALHDIQQTIQRLGMPNAPLQVNCLPSFALQWLMPRLTDFHREQPD
EECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCC
VSVRLKAEFQALDRQSMDTEDIDVAVRYDPVRYSRLHADVLLDEYLIPVATPEYLAEHRE
EEEEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAEGTSLDGVVLLHDASPWVGAAEFVEWRTWLEAFHPTWMAHLEGPQFNLSSLAISAALN
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHC
HQGVAMGRTALVYDEIRSGRLVNVFRQHVRAPARYMLLSRNPDDRRVTIFSAWLKAECAR
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHEEEEECCCCCCEEEEEHHHHHHHHHH
FDAMRMETLSRDR
HHHHHHHHHHCCC
>Mature Secondary Structure
MQIDSSILGALRCFEAAGRLLSFTKAAESLNLTQSAVSQQIRHLEDRLGYVLFVRQPRSL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
KLTPQGEVLFETTTRALHDIQQTIQRLGMPNAPLQVNCLPSFALQWLMPRLTDFHREQPD
EECCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCC
VSVRLKAEFQALDRQSMDTEDIDVAVRYDPVRYSRLHADVLLDEYLIPVATPEYLAEHRE
EEEEEEHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LAEGTSLDGVVLLHDASPWVGAAEFVEWRTWLEAFHPTWMAHLEGPQFNLSSLAISAALN
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHHHHC
HQGVAMGRTALVYDEIRSGRLVNVFRQHVRAPARYMLLSRNPDDRRVTIFSAWLKAECAR
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHEEEEECCCCCCEEEEEHHHHHHHHHH
FDAMRMETLSRDR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]