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The map label for this gene is amiA [C]

Identifier: 73542595

GI number: 73542595

Start: 3187081

End: 3187860

Strand: Reverse

Name: amiA [C]

Synonym: Reut_A2911

Alternate gene names: 73542595

Gene position: 3187860-3187081 (Counterclockwise)

Preceding gene: 73542596

Following gene: 73542594

Centisome position: 83.75

GC content: 65.38

Gene sequence:

>780_bases
ATGGCGCGGACGATCTCAACGGGCCGCCGCGCCGCATTGGCATGGCTGGCGGCTTGTGTGCCGATGCTGGCCACCGCATC
GCCGCTGTCCTCCGGTGCGCGGGCGTTCCGGATCGTCATCGATCCCGGCCACACGCCGGCGCAGGGTGGAGCGCTAGGCG
TTCGCGGCGTCTATGAGGTGCGCTACAACGATCGGCTGGCGTCGCAGGTGGCACAGGCGCTCACGCAGGCAGGGTTCGAC
GTGACGTTGACCCGTGGTCCGGACGACAACTTGTCGCTCGAGGAACGCGCCCGGATCGCCAATGCACGGCAGGCGGACCT
GTTCCTGTCCATTCACCACGACTCGGCGCAGATGCAGTATCTCGAAAAGGTGAGGGTGGAGGACCGTGACGCGTACCGGA
CCACGCGGCCGATCGCTGGGTATTCAGTTTTCGTATCGCAGCGCAATCCGAGCTTCGCGGGAAGCTACGCTTTCGCGCAA
ATGCTGGGCGAGGAAATGCGCAAGCTGGGCAGGGCGCCGACGCTGCATCATGCGGAGCCGATTGCCGGGGAGTCGCGGGA
ATTGCTGGCGCCCGATATCGGGATCTATCGATTCGATGATCTCGCTGTGCTGCGGCACACCGCCATGCCGGCTGTGTTGC
TGGAGGCGGGCGTGATCGTGGATCCCGGTGATGAGGGGTATGTCAGTGATCCGGGGAGTCAGGCGAGCATGGCCAATGCG
GTTGTCACGGCGGTTCGGCGGTATGTGGCGAGGGTTCGATCGGGCGACTTGCAGCGGTGA

Upstream 100 bases:

>100_bases
AACCGCGACCGCGCGGAGTACCTGCGCGACCGCGTGCGCGAGTGCAAGGCGGGCACCTGCCGCAACGACATGATCAGCAA
GAAGTCCTGGAACTGACGCC

Downstream 100 bases:

>100_bases
CAAAAGGACGACACGGGAAATCCGCAATTGCCGATCCGGCTTGATCGACGCAAAATCGGGCCACAACACTACGGCAGCTC
CAGGCGCTGCACGTTTTCTC

Product: cell wall hydrolase/autolysin

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: Cell Wall Hydrolase/Autolysin; Potential Peptidoglycan Amidase C-Terminal Fragment; N-Acetylmuramoyl-L-Alanine Amidase Family Protein

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MARTISTGRRAALAWLAACVPMLATASPLSSGARAFRIVIDPGHTPAQGGALGVRGVYEVRYNDRLASQVAQALTQAGFD
VTLTRGPDDNLSLEERARIANARQADLFLSIHHDSAQMQYLEKVRVEDRDAYRTTRPIAGYSVFVSQRNPSFAGSYAFAQ
MLGEEMRKLGRAPTLHHAEPIAGESRELLAPDIGIYRFDDLAVLRHTAMPAVLLEAGVIVDPGDEGYVSDPGSQASMANA
VVTAVRRYVARVRSGDLQR

Sequences:

>Translated_259_residues
MARTISTGRRAALAWLAACVPMLATASPLSSGARAFRIVIDPGHTPAQGGALGVRGVYEVRYNDRLASQVAQALTQAGFD
VTLTRGPDDNLSLEERARIANARQADLFLSIHHDSAQMQYLEKVRVEDRDAYRTTRPIAGYSVFVSQRNPSFAGSYAFAQ
MLGEEMRKLGRAPTLHHAEPIAGESRELLAPDIGIYRFDDLAVLRHTAMPAVLLEAGVIVDPGDEGYVSDPGSQASMANA
VVTAVRRYVARVRSGDLQR
>Mature_258_residues
ARTISTGRRAALAWLAACVPMLATASPLSSGARAFRIVIDPGHTPAQGGALGVRGVYEVRYNDRLASQVAQALTQAGFDV
TLTRGPDDNLSLEERARIANARQADLFLSIHHDSAQMQYLEKVRVEDRDAYRTTRPIAGYSVFVSQRNPSFAGSYAFAQM
LGEEMRKLGRAPTLHHAEPIAGESRELLAPDIGIYRFDDLAVLRHTAMPAVLLEAGVIVDPGDEGYVSDPGSQASMANAV
VTAVRRYVARVRSGDLQR

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 28077; Mature: 27946

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTISTGRRAALAWLAACVPMLATASPLSSGARAFRIVIDPGHTPAQGGALGVRGVYEV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
RYNDRLASQVAQALTQAGFDVTLTRGPDDNLSLEERARIANARQADLFLSIHHDSAQMQY
EHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHH
LEKVRVEDRDAYRTTRPIAGYSVFVSQRNPSFAGSYAFAQMLGEEMRKLGRAPTLHHAEP
HHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IAGESRELLAPDIGIYRFDDLAVLRHTAMPAVLLEAGVIVDPGDEGYVSDPGSQASMANA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHH
VVTAVRRYVARVRSGDLQR
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARTISTGRRAALAWLAACVPMLATASPLSSGARAFRIVIDPGHTPAQGGALGVRGVYEV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
RYNDRLASQVAQALTQAGFDVTLTRGPDDNLSLEERARIANARQADLFLSIHHDSAQMQY
EHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHH
LEKVRVEDRDAYRTTRPIAGYSVFVSQRNPSFAGSYAFAQMLGEEMRKLGRAPTLHHAEP
HHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IAGESRELLAPDIGIYRFDDLAVLRHTAMPAVLLEAGVIVDPGDEGYVSDPGSQASMANA
CCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHH
VVTAVRRYVARVRSGDLQR
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA