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The map label for this gene is cynR [H]

Identifier: 73542594

GI number: 73542594

Start: 3185989

End: 3186927

Strand: Reverse

Name: cynR [H]

Synonym: Reut_A2910

Alternate gene names: 73542594

Gene position: 3186927-3185989 (Counterclockwise)

Preceding gene: 73542595

Following gene: 73542593

Centisome position: 83.72

GC content: 67.94

Gene sequence:

>939_bases
GTGACGTCCACCGCCCCCTCACGCTCTTCCCGCCTGCACTTCGACCTCACCACCATCCAGCTCTTCATCGCCGTAGCAGA
CCAGGGCAGCATCACCCGCGGCGCCCAGCGCCTGCACCTGGCCGCTGCCGCCGCTTCCCGCCGCATCCTCGAACTGGAAT
CTCAGCTCGGGGTTTCGCTTTTCGAACGGCTTCCGCACGGCATGGCTTTGACCGAGGCCGGCCGCGCCTTGCTGGCCCAC
GCGCGTGGTATCACCCACACCGTCCAGCGCATGCAGGACGATGCGTCTTCGTTCGTCGGCGGCGACCTGGGTGTGGTGCG
CGTGGCGGCGCCGAAGTCCGCGGTGATCCAGTTTCTCCCGTTCGATATCCAGCGCTGCGCCGCCGCCTGTCCTGGCGTCC
GCATCGATCTCCAGGAGATGAACAGCCAGGAAGTCCAGCAATCGCTGCGACGGGGAACCGTGGATATCGGCATCTATGAG
GGCAGCCTCGGCGTGGTCGACATGCCGACGGAGCCGTATCGCGCCGATCGGCTGGTGCTGGTCGTTGCGCGCGGACACGG
GCTGGCGCGCCGCAAGCAGGTGACGACGGAGGATGTGCTGGACTGCGATCTGATCGTGCTCGGCGAAAGCTCGGCGATCT
CGATCGGGCTGGAGCGGCTGGCAGAGGAGGCCGGTCGCGTGCTGCGCATGCGCATGCGGGTCGGGGGCTTCGATAGCATC
GCCGCGCTGGTGGCGCAGAACCTCGGAGGCGGGGTGATGCCGGAGGCCATCGCGCGCGAGGTTGCCGGTGGCAGCCGCTT
TGCGCGGCTGCCCATCGCCGGGGATTGGGCGGAGCGGCAGTTTGTGCTGTGCCATCGACCGCATGGCGCGTTGTCCTCGG
CCGCGCAGAGCGTGGTGGCGGTGCTTGCGCAGAACGCGAAACCTGAATCACCCAAATAG

Upstream 100 bases:

>100_bases
AATCGGGCCACAACACTACGGCAGCTCCAGGCGCTGCACGTTTTCTCTCCCGGAACAGGCAGTTCGCTTCCCAACGGCGC
CCGCTCGAGCGCAGACCATC

Downstream 100 bases:

>100_bases
CGATAGCCCGCGGTGAAGGCATTGCGCATCATGGACTCCATAAAACGATCAGGACGGAGACACGCAATGCAGCGCAGGCA
ATTCATTTTCGCGGCTGCGG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTSTAPSRSSRLHFDLTTIQLFIAVADQGSITRGAQRLHLAAAAASRRILELESQLGVSLFERLPHGMALTEAGRALLAH
ARGITHTVQRMQDDASSFVGGDLGVVRVAAPKSAVIQFLPFDIQRCAAACPGVRIDLQEMNSQEVQQSLRRGTVDIGIYE
GSLGVVDMPTEPYRADRLVLVVARGHGLARRKQVTTEDVLDCDLIVLGESSAISIGLERLAEEAGRVLRMRMRVGGFDSI
AALVAQNLGGGVMPEAIAREVAGGSRFARLPIAGDWAERQFVLCHRPHGALSSAAQSVVAVLAQNAKPESPK

Sequences:

>Translated_312_residues
MTSTAPSRSSRLHFDLTTIQLFIAVADQGSITRGAQRLHLAAAAASRRILELESQLGVSLFERLPHGMALTEAGRALLAH
ARGITHTVQRMQDDASSFVGGDLGVVRVAAPKSAVIQFLPFDIQRCAAACPGVRIDLQEMNSQEVQQSLRRGTVDIGIYE
GSLGVVDMPTEPYRADRLVLVVARGHGLARRKQVTTEDVLDCDLIVLGESSAISIGLERLAEEAGRVLRMRMRVGGFDSI
AALVAQNLGGGVMPEAIAREVAGGSRFARLPIAGDWAERQFVLCHRPHGALSSAAQSVVAVLAQNAKPESPK
>Mature_311_residues
TSTAPSRSSRLHFDLTTIQLFIAVADQGSITRGAQRLHLAAAAASRRILELESQLGVSLFERLPHGMALTEAGRALLAHA
RGITHTVQRMQDDASSFVGGDLGVVRVAAPKSAVIQFLPFDIQRCAAACPGVRIDLQEMNSQEVQQSLRRGTVDIGIYEG
SLGVVDMPTEPYRADRLVLVVARGHGLARRKQVTTEDVLDCDLIVLGESSAISIGLERLAEEAGRVLRMRMRVGGFDSIA
ALVAQNLGGGVMPEAIAREVAGGSRFARLPIAGDWAERQFVLCHRPHGALSSAAQSVVAVLAQNAKPESPK

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=27.4647887323944, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=24.6527777777778, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788481, Length=238, Percent_Identity=28.9915966386555, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082132, Length=311, Percent_Identity=23.7942122186495, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788887, Length=186, Percent_Identity=28.494623655914, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=29.2490118577075, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787879, Length=293, Percent_Identity=24.5733788395904, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789204, Length=285, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87081904, Length=198, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=269, Percent_Identity=23.4200743494424, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=271, Percent_Identity=24.7232472324723, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33340; Mature: 33209

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTAPSRSSRLHFDLTTIQLFIAVADQGSITRGAQRLHLAAAAASRRILELESQLGVSL
CCCCCCCCCCEEEEEHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FERLPHGMALTEAGRALLAHARGITHTVQRMQDDASSFVGGDLGVVRVAAPKSAVIQFLP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCC
FDIQRCAAACPGVRIDLQEMNSQEVQQSLRRGTVDIGIYEGSLGVVDMPTEPYRADRLVL
CCHHHHHHHCCCCEEEHHHCCHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCEEEE
VVARGHGLARRKQVTTEDVLDCDLIVLGESSAISIGLERLAEEAGRVLRMRMRVGGFDSI
EEECCCCCHHHHCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AALVAQNLGGGVMPEAIAREVAGGSRFARLPIAGDWAERQFVLCHRPHGALSSAAQSVVA
HHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
VLAQNAKPESPK
HHHHCCCCCCCC
>Mature Secondary Structure 
TSTAPSRSSRLHFDLTTIQLFIAVADQGSITRGAQRLHLAAAAASRRILELESQLGVSL
CCCCCCCCCEEEEEHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FERLPHGMALTEAGRALLAHARGITHTVQRMQDDASSFVGGDLGVVRVAAPKSAVIQFLP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHCC
FDIQRCAAACPGVRIDLQEMNSQEVQQSLRRGTVDIGIYEGSLGVVDMPTEPYRADRLVL
CCHHHHHHHCCCCEEEHHHCCHHHHHHHHHCCCEEEEEEECCCCEEECCCCCCCCCEEEE
VVARGHGLARRKQVTTEDVLDCDLIVLGESSAISIGLERLAEEAGRVLRMRMRVGGFDSI
EEECCCCCHHHHCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AALVAQNLGGGVMPEAIAREVAGGSRFARLPIAGDWAERQFVLCHRPHGALSSAAQSVVA
HHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
VLAQNAKPESPK
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]