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The map label for this gene is pntB [H]

Identifier: 73542511

GI number: 73542511

Start: 3097415

End: 3098890

Strand: Direct

Name: pntB [H]

Synonym: Reut_A2826

Alternate gene names: 73542511

Gene position: 3097415-3098890 (Clockwise)

Preceding gene: 73542510

Following gene: 73542512

Centisome position: 81.37

GC content: 64.43

Gene sequence:

>1476_bases
ATGACCGAACTCGTCAGCATGAACCTTGTCACCCTGCTCTACCTGATCGCCTCGGTCTGTTTCATCCAGGCGCTCAAGGG
GCTTTCGCACCCGGCTTCGGCCCGCAAGGGCAATGCCTTCGGCATGATCGGCATGGCCATTGCCGTGGTCACTACGCTGG
TGCTGATCATCAAGCTCAAGAACGAGTTCCTCGCGGCCGGCACGGCGCAGTCCTCGGTGGGTTCGGGCCTGGCGCTGATC
TTCGGCGCGCTGGTCGTGGGCGGCGGCATTGGCGCCTATGTGGCGAAGAAGGTCGAGATGACCAAGATGCCCGAACTGGT
CGCGGCGATGCACTCGCTGATCGGTCTCGCGGCGGTGTTCATCGCGGTGGCCGCGGTGGCCGAGCCTTCGGCCTTCGGCA
TAGCGCCGGCGGGCTCGCATGAGATCCCGCTGGGCAACCGCATCGAACTGTTCATCGGCTGCTTCGTCGGTGCCATCACC
TTCTCCGGTTCGGTGATCGCCTTCGGCAAGCTGGCCGGGCGCTACAAGTTCCGCCTGTTCCAGGGTGCGCCGGTGGTGTT
CGCGGGCCAGCACATGCTGAACCTGGCGCTCGCCGTCGCCATGGTCGGCTTCGGCATCGCGTTCTTCATGACGCAGGAGT
GGCTGCCGTTCCTCGTCATGCTGGCCATTGCCTTCGTGCTCGGCGTGCTGATCATCATCCCGATCGGCGGCGCCGACATG
CCGGTGGTGGTGTCGATGCTGAACTCGTACTCGGGCTGGGCGGCGGCAGGCATCGGCTTCTCGCTGAACAACCCGATGCT
GATCATTGCCGGTTCGCTGGTGGGTTCGTCCGGTGCCATCCTCTCGTACATCATGTGCAAGGCGATGAACCGCTCGTTCT
TCAACGTGATCCTGGGCGGATTCGGCGGCGAGGCGGCGGCGGCCGGCGCGGCCGGGGCACAGGCCCAGCGCAACGTGAAG
TCGGGCTCCGCTGACGATGCGGCCTTCCTGATGGGCAATGCCGAGACCGTGATCATCGTGCCGGGCTATGGCCTGGCGGT
GGCACGGGCCCAGCATGCGCTCAAGGAGCTGACCGAGAAGCTGACCGAGAAGGGCGTGACGGTGAAGTACGCGATCCACC
CGGTAGCGGGCCGCATGCCGGGCCACATGAACGTGCTGCTGGCCGAAGCCGAAGTGCCGTACGACATGGTCTTCGAGATG
GAAGACATCAACAGCGAGTTCGGCCAGGCCGACGTGGTGCTGGTGCTCGGCGCCAACGACGTGGTCAACCCGTCGGCCAA
GACCGATCCGAAGTCGCCGATCGCCGGCATGCCGATCCTGGAGGCGTACAAGGCCAAGACCATCATCGTGAACAAGCGAT
CGATGGCGGCCGGCTACGCGGGCCTGGACAACGAGCTGTTCTACATGGACAAGACCATGATGGTGTTCGGCGACGCCAAG
AAGGTGGTCGAGGACATGTTCAAGGCCGTGGAATAA

Upstream 100 bases:

>100_bases
CTGCCGTCAACGTGTTCGGCGGCTTCCTGGTGACCCAGCGGATGCTGGAGATGTTCAAGAAGAAAGAGCCGAAGGCCAAG
GCCGCCAAGGAGGGTGCGTG

Downstream 100 bases:

>100_bases
CCACGGGCGCTACAGTCGAATCGCGAATTCGTGCCTGATGCGCCACGCTGCCGCCCTTCGGGGCGGCAGCGCGTTATACT
GGCCTGCGCTGAATTCCCAG

Product: NAD(P) transhydrogenase, beta subunit

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTELVSMNLVTLLYLIASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLVLIIKLKNEFLAAGTAQSSVGSGLALI
FGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPSAFGIAPAGSHEIPLGNRIELFIGCFVGAIT
FSGSVIAFGKLAGRYKFRLFQGAPVVFAGQHMLNLALAVAMVGFGIAFFMTQEWLPFLVMLAIAFVLGVLIIIPIGGADM
PVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAAAAGAAGAQAQRNVK
SGSADDAAFLMGNAETVIIVPGYGLAVARAQHALKELTEKLTEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVFEM
EDINSEFGQADVVLVLGANDVVNPSAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAK
KVVEDMFKAVE

Sequences:

>Translated_491_residues
MTELVSMNLVTLLYLIASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLVLIIKLKNEFLAAGTAQSSVGSGLALI
FGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPSAFGIAPAGSHEIPLGNRIELFIGCFVGAIT
FSGSVIAFGKLAGRYKFRLFQGAPVVFAGQHMLNLALAVAMVGFGIAFFMTQEWLPFLVMLAIAFVLGVLIIIPIGGADM
PVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAAAAGAAGAQAQRNVK
SGSADDAAFLMGNAETVIIVPGYGLAVARAQHALKELTEKLTEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVFEM
EDINSEFGQADVVLVLGANDVVNPSAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAK
KVVEDMFKAVE
>Mature_490_residues
TELVSMNLVTLLYLIASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLVLIIKLKNEFLAAGTAQSSVGSGLALIF
GALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPSAFGIAPAGSHEIPLGNRIELFIGCFVGAITF
SGSVIAFGKLAGRYKFRLFQGAPVVFAGQHMLNLALAVAMVGFGIAFFMTQEWLPFLVMLAIAFVLGVLIIIPIGGADMP
VVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGGEAAAAGAAGAQAQRNVKS
GSADDAAFLMGNAETVIIVPGYGLAVARAQHALKELTEKLTEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVFEME
DINSEFGQADVVLVLGANDVVNPSAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKK
VVEDMFKAVE

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=486, Percent_Identity=46.0905349794239, Blast_Score=404, Evalue=1e-112,
Organism=Homo sapiens, GI122939153, Length=486, Percent_Identity=46.0905349794239, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI1787886, Length=486, Percent_Identity=51.0288065843621, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17550456, Length=483, Percent_Identity=42.6501035196687, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 51178; Mature: 51047

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELVSMNLVTLLYLIASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLVLIIKLK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NEFLAAGTAQSSVGSGLALIFGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVF
HHHHCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVAAVAEPSAFGIAPAGSHEIPLGNRIELFIGCFVGAITFSGSVIAFGKLAGRYKFRLF
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCEEEEEEE
QGAPVVFAGQHMLNLALAVAMVGFGIAFFMTQEWLPFLVMLAIAFVLGVLIIIPIGGADM
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCH
PVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGG
HHHHHHHHCCCCCEEECCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCC
FGGEAAAAGAAGAQAQRNVKSGSADDAAFLMGNAETVIIVPGYGLAVARAQHALKELTEK
CCCCHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
LTEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVFEMEDINSEFGQADVVLVLGAND
HHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHEEEHHHHCCCCCCCCEEEEECCCC
VVNPSAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAK
CCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHCCCCCCCCCEEEECCEEEEECCHH
KVVEDMFKAVE
HHHHHHHHHCC
>Mature Secondary Structure 
TELVSMNLVTLLYLIASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLVLIIKLK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
NEFLAAGTAQSSVGSGLALIFGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVF
HHHHCCCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAVAAVAEPSAFGIAPAGSHEIPLGNRIELFIGCFVGAITFSGSVIAFGKLAGRYKFRLF
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCEEEEEEE
QGAPVVFAGQHMLNLALAVAMVGFGIAFFMTQEWLPFLVMLAIAFVLGVLIIIPIGGADM
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCH
PVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGG
HHHHHHHHCCCCCEEECCCEECCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHCC
FGGEAAAAGAAGAQAQRNVKSGSADDAAFLMGNAETVIIVPGYGLAVARAQHALKELTEK
CCCCHHHCCCCCHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
LTEKGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDMVFEMEDINSEFGQADVVLVLGAND
HHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHEEEHHHHCCCCCCCCEEEEECCCC
VVNPSAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAK
CCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCHHHCCCCCCCCCEEEECCEEEEECCHH
KVVEDMFKAVE
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]