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The map label for this gene is panB

Identifier: 73542465

GI number: 73542465

Start: 3048675

End: 3049496

Strand: Direct

Name: panB

Synonym: Reut_A2780

Alternate gene names: 73542465

Gene position: 3048675-3049496 (Clockwise)

Preceding gene: 73542464

Following gene: 73542466

Centisome position: 80.09

GC content: 65.45

Gene sequence:

>822_bases
ATGAGCTACCTCCTCGATCCCTCCCGCAAGACCGTCACCATCACCCGGCTGCAGGCCATGCGCGACGCCGGTGAAAAGAT
CGCGATGCTGACCGCTTACGACTCCAGCTTCGCCGCGTTGCTCGACTACTGCGGCGTGGAAGTCATCCTGGTCGGTGATT
CGCTCGGCAACGTGATGCAAGGCCAGCAAACCACGCTGCCCGTGACGCTGGAGCACATGGCTTACCACACCGAATGCGTG
GCACGCGCCAACCAGACGGCGCTGCTGGTAACCGACCTGCCCTTCGGCACCTACGGCACGCCGGAAATGGCATTTGCAAG
CGCGGTCACGCTGATGCGTGCGGGCGCGCACATGGTCAAGCTCGAAGGCGGCGACTGGCTGGCCCCGACCGTGAAGTTCC
TGGTCGAGCGCAGCATCCCGGTGTGCGCCCATATCGGCCTGACCCCGCAGTCGGTGCATGCGTTCGGCGGCTTCAAGGTG
CAGGGCAAGACCGATGAAGGCGCAGCCCAACTGCGGCGCGACGCTCAGGCAATGGAGGCTGCCGGCGCACAGATCGTGCT
GATGGAGGCGGTGCCGGCCACGCTCGCCGGCGAGATCACGCAGATGCTCAAGGTGCCGACCATCGGCATCGGTGCCGGCG
CGGACTGCTCGGGCCAGGTGCTGGTGCTGCAGGACATGATCAACGTCTACCCGGGACGCAAGGCCAAGTTCGTGCGCAAC
TTCATGGACGGCCAGACGACGATCGAAGGTGCGATCCGCGCCTATGTGGCCGCCGTGAAGGATGGCAGCTTCCCGGCAGC
AGAGCACACCTTCTCCGCCTGA

Upstream 100 bases:

>100_bases
GCCCTGACCGCAGCGGGCTTGCCCGCGACGGCACACAACCATAACGACAGCCGCGCCGGCCCGCCTCACGCCGAGCGCCC
TCTCCTGACTCGTCACTGCC

Downstream 100 bases:

>100_bases
CCGGTGGAGATCGGCGCCGCCACGCTGGCCGCGTTCCGCCTGCTTGCCAGCGGCGACGCGGCGCTCTGGTTCATTGTCTG
GACCTCGCTGATGGTCGCGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSYLLDPSRKTVTITRLQAMRDAGEKIAMLTAYDSSFAALLDYCGVEVILVGDSLGNVMQGQQTTLPVTLEHMAYHTECV
ARANQTALLVTDLPFGTYGTPEMAFASAVTLMRAGAHMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHAFGGFKV
QGKTDEGAAQLRRDAQAMEAAGAQIVLMEAVPATLAGEITQMLKVPTIGIGAGADCSGQVLVLQDMINVYPGRKAKFVRN
FMDGQTTIEGAIRAYVAAVKDGSFPAAEHTFSA

Sequences:

>Translated_273_residues
MSYLLDPSRKTVTITRLQAMRDAGEKIAMLTAYDSSFAALLDYCGVEVILVGDSLGNVMQGQQTTLPVTLEHMAYHTECV
ARANQTALLVTDLPFGTYGTPEMAFASAVTLMRAGAHMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHAFGGFKV
QGKTDEGAAQLRRDAQAMEAAGAQIVLMEAVPATLAGEITQMLKVPTIGIGAGADCSGQVLVLQDMINVYPGRKAKFVRN
FMDGQTTIEGAIRAYVAAVKDGSFPAAEHTFSA
>Mature_272_residues
SYLLDPSRKTVTITRLQAMRDAGEKIAMLTAYDSSFAALLDYCGVEVILVGDSLGNVMQGQQTTLPVTLEHMAYHTECVA
RANQTALLVTDLPFGTYGTPEMAFASAVTLMRAGAHMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHAFGGFKVQ
GKTDEGAAQLRRDAQAMEAAGAQIVLMEAVPATLAGEITQMLKVPTIGIGAGADCSGQVLVLQDMINVYPGRKAKFVRNF
MDGQTTIEGAIRAYVAAVKDGSFPAAEHTFSA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=51.5267175572519, Blast_Score=276, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319653, Length=282, Percent_Identity=35.4609929078014, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CUPPJ (Q46XJ2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296985.1
- ProteinModelPortal:   Q46XJ2
- SMR:   Q46XJ2
- GeneID:   3612311
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2780
- NMPDR:   fig|264198.3.peg.3386
- HOGENOM:   HBG299908
- OMA:   AKFVRNF
- ProtClustDB:   PRK00311
- BioCyc:   REUT264198:REUT_A2780-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29015; Mature: 28884

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 92-92 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLLDPSRKTVTITRLQAMRDAGEKIAMLTAYDSSFAALLDYCGVEVILVGDSLGNVMQ
CCCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCEEEEEEECCCCHHHC
GQQTTLPVTLEHMAYHTECVARANQTALLVTDLPFGTYGTPEMAFASAVTLMRAGAHMVK
CCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHAFGGFKVQGKTDEGAAQLRRDAQAMEA
ECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQIVLMEAVPATLAGEITQMLKVPTIGIGAGADCSGQVLVLQDMINVYPGRKAKFVRN
CCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEHHHHHHHCCCCHHHHHHH
FMDGQTTIEGAIRAYVAAVKDGSFPAAEHTFSA
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SYLLDPSRKTVTITRLQAMRDAGEKIAMLTAYDSSFAALLDYCGVEVILVGDSLGNVMQ
CCCCCCCCCEEEHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCEEEEEEECCCCHHHC
GQQTTLPVTLEHMAYHTECVARANQTALLVTDLPFGTYGTPEMAFASAVTLMRAGAHMVK
CCCCCCCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEE
LEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHAFGGFKVQGKTDEGAAQLRRDAQAMEA
ECCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHH
AGAQIVLMEAVPATLAGEITQMLKVPTIGIGAGADCSGQVLVLQDMINVYPGRKAKFVRN
CCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEHHHHHHHCCCCHHHHHHH
FMDGQTTIEGAIRAYVAAVKDGSFPAAEHTFSA
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA