| Definition | Ralstonia eutropha JMP134 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007347 |
| Length | 3,806,533 |
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The map label for this gene is cphA [H]
Identifier: 73542270
GI number: 73542270
Start: 2832484
End: 2835120
Strand: Direct
Name: cphA [H]
Synonym: Reut_A2585
Alternate gene names: 73542270
Gene position: 2832484-2835120 (Clockwise)
Preceding gene: 73542267
Following gene: 73542271
Centisome position: 74.41
GC content: 67.31
Gene sequence:
>2637_bases ATGAAAAAGAAGGACATTGAGATTTTCGATGTCATGTCGCTGCGCGGCCCGAATATGTGGACATATCGGCCCGTGCTGGA GGCGTGGGTCGATATCGGGGAGCTGGAGGATTTTCCCTCCAACACGATTCCGGGGTTCTATGAACGGCTGTCGACGTGGC TGCCCTCGCTAGTCGAGCATCGCTGCAGCATCGGCGAACGCGGCGGCTTCCTGCAGCGCCTCAAGGAAGGCACGTGGCCG GGTCATATCCTGGAACACGTGACGCTTGAGCTGCAGAACCTGGCTGGCATGCCCGGCGGCTTCGGCAAGGCGCGCGAGAC CCCCATCCGCGGCGTCTACAAGGTGATCGTGCGCGCCTGGCATGAGGAAGTCACGCGCGCCGCGCTGTTCGCGGCCCGCG ACCTGGTCATGGCCGCCATCGAGGACCGTCCGTTCGACGTGCCCGCCGCTGTGGACAACCTGCGCCGCCTGGTCGACGAG CACTGCCTCGGCCCGAGCACGGCCTGCATCGTCGATGCCGCGGACGACCGCGACATCCCGTCGATCCGCCTGTCCGACGG CAACCTCGTGCAGCTTGGCTACGGCGCGCGCTCGCGCCGTATCTGGACGGCCGAGACCGATCGCACCAGCGCCATCGCCG AAAGCATCTCGCGCGACAAGGACCTGACCAAGAGCCTGCTGGAATCGTGCGGCGTGCCGGTGCCGGAAGGCCGCATGGTC GAGTCGCCCGAAGACGCGTGGGAAGCCGCCGAGGACATCGGGGTGCCGGTGGTGGTCAAGCCGTACGACGGCAACCACGG CCGCGGCGTCTTCACCAACCTGATGACGCGCGAGGAAGTCGAGACCGCCTATGCGGTAGCCATCGAGGAAGGCAGCGGGG TCATCGTCGAGCGTTTTGTGCCCGGCAACGAACACCGCCTGCTGGTAGTCGGCGGCCGCGTGGTCGCCGCCGCGATGGGC GAGACTGCGAGCGTGGTGGGCGACGGCAAGTCGACCATCGAGGAACTGATCGAACTGCAAATCAACTCGGATCCGCGCCG TGGCAGCACCGAAGATCATCCGCTGAACCGCGTCCGCCTGGACTCGGCAGCGCGCCTGGAACTCAAGCGCCAGAACATGG ATGGCGAGTCGGTACCTCCGGCAGGCAAGACCGTGCTGATCCAGCGCACCGGCAATGTGGCGTTCGACGTGACCGACCGC GTACACCCCAGCGTGGCCGCTCATGCGGCGCTGGCTGCGCGCGTGGTCGGCCTGGACATCGCCGGCGTGGATCTGGTGGC CGAAGACATTTCGCGCCCGCTGACCGACCAGCGCGGGGCCATCGTCGAAGTCAACGCCGGCCCCGGCCTGCTCATGCACC TCAAGCCGGCCGAAGGCACGCCGCGCCCGGTGGGCCGCGCCATCGTCGATCACCTGTTCCCCGAGGCCGACGACACGTAT CCCGGCCGCATCCCCGTGGTCGGCATTACCGGCACCAACGGCAAGACCGTGGTGGCCAAGCTGGTCGCGCGCCTGCTGCA ACTGTCGGGCAAGCACACCGGCCTGGCGTGCAGCGACGGCCTGTTCCTGGATCGTCGCCTGGTCCAGGGCGGCGACCGCG CCAACTGGGACGCCGGCCATCGCATCCTGATGAACCGTGCGGTCGAAGCCGCCGTGTTCGAGAACGACAGCGGCGCCATC CTGTCCGAAGGTCTCGTCTATGACCGCTGCCAGGTTGGCGTGGTCACGAACTTCGACACGCCCGACCACATGGGCGATTA CTACGTCGAAGACGAAGACCGCATGTACAACGTGCTGCGCACGCAGATCGACGTGGTCCTCAAGAACGGCGCCGCCGTGC TCAACGCGCGCGACGAGCGCGTGGTCGAAATGGCCGAGCTGTCCGACGGCGACGTGATCTTCTTCGGCCTGTCGGCAGAT CTGCCGGCCATCGTCGCGCATCGCGCCAAGGGCAAGCGCGCGGTATTCGTGCGCGATGGCAAGGTCGTGCTGACCACCGG CAACAGTGAAACGCCGCTGACCGATGTCGCCGCGATTCCGGTGACTTATGCCGGCCGCGTGTCGTTCCAGGTCGAGAACG TGCTGGCGGCCGTCGCTACAGGCTGGGCGCTGGGCATCTCCAATGACCTGATCCGCGCCGGCATCGTGACGTTCGACGTC GGCCAGGTCGATGTACCGGGGCGCTTCACGCTGTTCGAGCGCAATGGCGCCACGGTGATCGTGGACGATGCGCACAACGC CCCTGCCCTGGAAGCCTTGGCCACGGCTCTGGACCGCTTCCCGGCGGAACGCCGCATGATCGTCTACGGTGCCGGCGTAC AACGCCGCGACGAAGACATGGTCCGCCAGGGCAAGGTCATTGGCGCTACCTTCGACCGCGTGTTCCTGTGCGAGGACCGC AGCGTCAAGGGCGCGCTGCCCGATGCCGAGGCGCGCGCCCTGCTCAAGCAGGGGCTGTACGAAGGCCGCCGCGTCACCAA GATCATCGACGAAGGCACGCGCCAGGACGCCATCGAAACCGCGCTCGGCCAACTGGTGGCCGGCGACCTGCTGGTTCTGC AGTGCGACGAAGGTGCCACCGCCGCCACCGTCGACCGCGTGCACCAGTGGATGGGTCAGCCCCCGCGCCGCGCCTGA
Upstream 100 bases:
>100_bases ATGTCAGCTAAAGAAGTCAACCTGACGTATGCTGTAACGTTATTGAAAGGTTAGGCTTCCATTTCTCGGGCGGTCCCGCC CATTGCCTCAAGCTAAATCA
Downstream 100 bases:
>100_bases CGGCCCAAGAAGAACGGATACCAGAATCTATGGAAGTCTCTCGCATCCGGGCCCTGCGCGGCCCGAACCTGTGGTGCCGC CACACCGCCGTCGAGGCGAT
Product: cyanophycin synthetase
Products: NA
Alternate protein names: Cyanophycin synthase [H]
Number of amino acids: Translated: 878; Mature: 878
Protein sequence:
>878_residues MKKKDIEIFDVMSLRGPNMWTYRPVLEAWVDIGELEDFPSNTIPGFYERLSTWLPSLVEHRCSIGERGGFLQRLKEGTWP GHILEHVTLELQNLAGMPGGFGKARETPIRGVYKVIVRAWHEEVTRAALFAARDLVMAAIEDRPFDVPAAVDNLRRLVDE HCLGPSTACIVDAADDRDIPSIRLSDGNLVQLGYGARSRRIWTAETDRTSAIAESISRDKDLTKSLLESCGVPVPEGRMV ESPEDAWEAAEDIGVPVVVKPYDGNHGRGVFTNLMTREEVETAYAVAIEEGSGVIVERFVPGNEHRLLVVGGRVVAAAMG ETASVVGDGKSTIEELIELQINSDPRRGSTEDHPLNRVRLDSAARLELKRQNMDGESVPPAGKTVLIQRTGNVAFDVTDR VHPSVAAHAALAARVVGLDIAGVDLVAEDISRPLTDQRGAIVEVNAGPGLLMHLKPAEGTPRPVGRAIVDHLFPEADDTY PGRIPVVGITGTNGKTVVAKLVARLLQLSGKHTGLACSDGLFLDRRLVQGGDRANWDAGHRILMNRAVEAAVFENDSGAI LSEGLVYDRCQVGVVTNFDTPDHMGDYYVEDEDRMYNVLRTQIDVVLKNGAAVLNARDERVVEMAELSDGDVIFFGLSAD LPAIVAHRAKGKRAVFVRDGKVVLTTGNSETPLTDVAAIPVTYAGRVSFQVENVLAAVATGWALGISNDLIRAGIVTFDV GQVDVPGRFTLFERNGATVIVDDAHNAPALEALATALDRFPAERRMIVYGAGVQRRDEDMVRQGKVIGATFDRVFLCEDR SVKGALPDAEARALLKQGLYEGRRVTKIIDEGTRQDAIETALGQLVAGDLLVLQCDEGATAATVDRVHQWMGQPPRRA
Sequences:
>Translated_878_residues MKKKDIEIFDVMSLRGPNMWTYRPVLEAWVDIGELEDFPSNTIPGFYERLSTWLPSLVEHRCSIGERGGFLQRLKEGTWP GHILEHVTLELQNLAGMPGGFGKARETPIRGVYKVIVRAWHEEVTRAALFAARDLVMAAIEDRPFDVPAAVDNLRRLVDE HCLGPSTACIVDAADDRDIPSIRLSDGNLVQLGYGARSRRIWTAETDRTSAIAESISRDKDLTKSLLESCGVPVPEGRMV ESPEDAWEAAEDIGVPVVVKPYDGNHGRGVFTNLMTREEVETAYAVAIEEGSGVIVERFVPGNEHRLLVVGGRVVAAAMG ETASVVGDGKSTIEELIELQINSDPRRGSTEDHPLNRVRLDSAARLELKRQNMDGESVPPAGKTVLIQRTGNVAFDVTDR VHPSVAAHAALAARVVGLDIAGVDLVAEDISRPLTDQRGAIVEVNAGPGLLMHLKPAEGTPRPVGRAIVDHLFPEADDTY PGRIPVVGITGTNGKTVVAKLVARLLQLSGKHTGLACSDGLFLDRRLVQGGDRANWDAGHRILMNRAVEAAVFENDSGAI LSEGLVYDRCQVGVVTNFDTPDHMGDYYVEDEDRMYNVLRTQIDVVLKNGAAVLNARDERVVEMAELSDGDVIFFGLSAD LPAIVAHRAKGKRAVFVRDGKVVLTTGNSETPLTDVAAIPVTYAGRVSFQVENVLAAVATGWALGISNDLIRAGIVTFDV GQVDVPGRFTLFERNGATVIVDDAHNAPALEALATALDRFPAERRMIVYGAGVQRRDEDMVRQGKVIGATFDRVFLCEDR SVKGALPDAEARALLKQGLYEGRRVTKIIDEGTRQDAIETALGQLVAGDLLVLQCDEGATAATVDRVHQWMGQPPRRA >Mature_878_residues MKKKDIEIFDVMSLRGPNMWTYRPVLEAWVDIGELEDFPSNTIPGFYERLSTWLPSLVEHRCSIGERGGFLQRLKEGTWP GHILEHVTLELQNLAGMPGGFGKARETPIRGVYKVIVRAWHEEVTRAALFAARDLVMAAIEDRPFDVPAAVDNLRRLVDE HCLGPSTACIVDAADDRDIPSIRLSDGNLVQLGYGARSRRIWTAETDRTSAIAESISRDKDLTKSLLESCGVPVPEGRMV ESPEDAWEAAEDIGVPVVVKPYDGNHGRGVFTNLMTREEVETAYAVAIEEGSGVIVERFVPGNEHRLLVVGGRVVAAAMG ETASVVGDGKSTIEELIELQINSDPRRGSTEDHPLNRVRLDSAARLELKRQNMDGESVPPAGKTVLIQRTGNVAFDVTDR VHPSVAAHAALAARVVGLDIAGVDLVAEDISRPLTDQRGAIVEVNAGPGLLMHLKPAEGTPRPVGRAIVDHLFPEADDTY PGRIPVVGITGTNGKTVVAKLVARLLQLSGKHTGLACSDGLFLDRRLVQGGDRANWDAGHRILMNRAVEAAVFENDSGAI LSEGLVYDRCQVGVVTNFDTPDHMGDYYVEDEDRMYNVLRTQIDVVLKNGAAVLNARDERVVEMAELSDGDVIFFGLSAD LPAIVAHRAKGKRAVFVRDGKVVLTTGNSETPLTDVAAIPVTYAGRVSFQVENVLAAVATGWALGISNDLIRAGIVTFDV GQVDVPGRFTLFERNGATVIVDDAHNAPALEALATALDRFPAERRMIVYGAGVQRRDEDMVRQGKVIGATFDRVFLCEDR SVKGALPDAEARALLKQGLYEGRRVTKIIDEGTRQDAIETALGQLVAGDLLVLQCDEGATAATVDRVHQWMGQPPRRA
Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]
COG id: COG1181
COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 ATP-grasp domain [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR011761 - InterPro: IPR013651 - InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR011810 - InterPro: IPR004101 - InterPro: IPR013221 [H]
Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]
EC number: =6.3.2.29; =6.3.2.30 [H]
Molecular weight: Translated: 95336; Mature: 95336
Theoretical pI: Translated: 4.94; Mature: 4.94
Prosite motif: PS50975 ATP_GRASP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKKDIEIFDVMSLRGPNMWTYRPVLEAWVDIGELEDFPSNTIPGFYERLSTWLPSLVEH CCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH RCSIGERGGFLQRLKEGTWPGHILEHVTLELQNLAGMPGGFGKARETPIRGVYKVIVRAW HHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH HEEVTRAALFAARDLVMAAIEDRPFDVPAAVDNLRRLVDEHCLGPSTACIVDAADDRDIP HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC SIRLSDGNLVQLGYGARSRRIWTAETDRTSAIAESISRDKDLTKSLLESCGVPVPEGRMV EEEECCCCEEEECCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC ESPEDAWEAAEDIGVPVVVKPYDGNHGRGVFTNLMTREEVETAYAVAIEEGSGVIVERFV CCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCEEEEEEC PGNEHRLLVVGGRVVAAAMGETASVVGDGKSTIEELIELQINSDPRRGSTEDHPLNRVRL CCCCCEEEEECCEEEEECCCCCCEEECCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEEC DSAARLELKRQNMDGESVPPAGKTVLIQRTGNVAFDVTDRVHPSVAAHAALAARVVGLDI CCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCEE AGVDLVAEDISRPLTDQRGAIVEVNAGPGLLMHLKPAEGTPRPVGRAIVDHLFPEADDTY CCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC PGRIPVVGITGTNGKTVVAKLVARLLQLSGKHTGLACSDGLFLDRRLVQGGDRANWDAGH CCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCCCHHH RILMNRAVEAAVFENDSGAILSEGLVYDRCQVGVVTNFDTPDHMGDYYVEDEDRMYNVLR HHHHHHHHEEEEEECCCCCEEECCCEECCEEEEEEECCCCCCCCCCEEECCHHHHHHHHH TQIDVVLKNGAAVLNARDERVVEMAELSDGDVIFFGLSADLPAIVAHRAKGKRAVFVRDG HHHHHHEECCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCEEEEEECC KVVLTTGNSETPLTDVAAIPVTYAGRVSFQVENVLAAVATGWALGISNDLIRAGIVTFDV EEEEECCCCCCCCHHHEECCEEECCEEEEEHHHHHHHHHHHHHCCCCCHHHHHCEEEEEC GQVDVPGRFTLFERNGATVIVDDAHNAPALEALATALDRFPAERRMIVYGAGVQRRDEDM CCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH VRQGKVIGATFDRVFLCEDRSVKGALPDAEARALLKQGLYEGRRVTKIIDEGTRQDAIET HHCCCEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH ALGQLVAGDLLVLQCDEGATAATVDRVHQWMGQPPRRA HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCC >Mature Secondary Structure MKKKDIEIFDVMSLRGPNMWTYRPVLEAWVDIGELEDFPSNTIPGFYERLSTWLPSLVEH CCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH RCSIGERGGFLQRLKEGTWPGHILEHVTLELQNLAGMPGGFGKARETPIRGVYKVIVRAW HHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH HEEVTRAALFAARDLVMAAIEDRPFDVPAAVDNLRRLVDEHCLGPSTACIVDAADDRDIP HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC SIRLSDGNLVQLGYGARSRRIWTAETDRTSAIAESISRDKDLTKSLLESCGVPVPEGRMV EEEECCCCEEEECCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC ESPEDAWEAAEDIGVPVVVKPYDGNHGRGVFTNLMTREEVETAYAVAIEEGSGVIVERFV CCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCEEEEEEC PGNEHRLLVVGGRVVAAAMGETASVVGDGKSTIEELIELQINSDPRRGSTEDHPLNRVRL CCCCCEEEEECCEEEEECCCCCCEEECCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEEC DSAARLELKRQNMDGESVPPAGKTVLIQRTGNVAFDVTDRVHPSVAAHAALAARVVGLDI CCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHCCEE AGVDLVAEDISRPLTDQRGAIVEVNAGPGLLMHLKPAEGTPRPVGRAIVDHLFPEADDTY CCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC PGRIPVVGITGTNGKTVVAKLVARLLQLSGKHTGLACSDGLFLDRRLVQGGDRANWDAGH CCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCCCHHH RILMNRAVEAAVFENDSGAILSEGLVYDRCQVGVVTNFDTPDHMGDYYVEDEDRMYNVLR HHHHHHHHEEEEEECCCCCEEECCCEECCEEEEEEECCCCCCCCCCEEECCHHHHHHHHH TQIDVVLKNGAAVLNARDERVVEMAELSDGDVIFFGLSADLPAIVAHRAKGKRAVFVRDG HHHHHHEECCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCEEEEEECC KVVLTTGNSETPLTDVAAIPVTYAGRVSFQVENVLAAVATGWALGISNDLIRAGIVTFDV EEEEECCCCCCCCHHHEECCEEECCEEEEEHHHHHHHHHHHHHCCCCCHHHHHCEEEEEC GQVDVPGRFTLFERNGATVIVDDAHNAPALEALATALDRFPAERRMIVYGAGVQRRDEDM CCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH VRQGKVIGATFDRVFLCEDRSVKGALPDAEARALLKQGLYEGRRVTKIIDEGTRQDAIET HHCCCEEEEECCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHH ALGQLVAGDLLVLQCDEGATAATVDRVHQWMGQPPRRA HHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11131019 [H]