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The map label for this gene is gph2 [H]

Identifier: 73542266

GI number: 73542266

Start: 2827787

End: 2828452

Strand: Direct

Name: gph2 [H]

Synonym: Reut_A2581

Alternate gene names: 73542266

Gene position: 2827787-2828452 (Clockwise)

Preceding gene: 73542265

Following gene: 73542267

Centisome position: 74.29

GC content: 65.77

Gene sequence:

>666_bases
ATGATGACTGGAATCGACGCCGTGTTTTTCGATCTCGACGGGACGCTCGCCGACACCGCTCCGGATCTTGCCGCAGCTGC
CAACCGGCTGGTCGTCGAGCATGGTGGCTCGCCTGTTGCCTATGAAAAGCTGCGCCCCGTGGCGTCGCATGGCGCGCGCG
GCCTGCTGGGCGCTGCATTCGGCAAGCACCCGGACGATCCGGACTTCCCTGCGCTGCGCGACCTGTTTCTCGACTACTAC
GAAGCCGATATCGCCGTGCATACTCGGCTGTTCGACGGCATGCCGCAGGTACTCGATGCGCTGGAAGGTGCAGGCATCCG
CTGGGGCATTGTGACCAACAAGATCGCCAGATTCACCGTGCCGCTCGTGACCGCGATCGGGCTGGCGCCGCGCGCGTCAG
CCGTGGTCAGCGGGGATACGACGCCCCATGCCAAGCCACATCCGGCTCCGCTGCTCCGTGCCGCAGAACTCAGTGGCGTA
TCGCCTAAGCGTTGCGTGTATGTGGGCGATGACTTGCGCGATATCCAGGCGGGCAAGGCAGCGGGCATGCTCACGGTCAC
GGCCGCGTACGGGTACTGCGGCGAAGGCGAACCGCCGGAGACCTGGGGCGCCGACTACCTCGTGCGCCACCCTGCCGAGT
TGATTCCGCTGCTCATGCCGGACTGA

Upstream 100 bases:

>100_bases
TGGAAATGCGCGGCATGACCTATAACCCGCTGTCGCAGATTTATTCACTGAGCCGTGATACGGACGTCAATTACATGATG
GCGTTTCGCCGGGTTGCAGA

Downstream 100 bases:

>100_bases
TCTCCCTCTGCAAGCAAAAACGGAACCCGGCGCGGCAACTTGCGTCCAACCCGGGTACAATCCGTCTTCTCATCACTGGG
GCCGACCTGGTTTCGACGTG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MMTGIDAVFFDLDGTLADTAPDLAAAANRLVVEHGGSPVAYEKLRPVASHGARGLLGAAFGKHPDDPDFPALRDLFLDYY
EADIAVHTRLFDGMPQVLDALEGAGIRWGIVTNKIARFTVPLVTAIGLAPRASAVVSGDTTPHAKPHPAPLLRAAELSGV
SPKRCVYVGDDLRDIQAGKAAGMLTVTAAYGYCGEGEPPETWGADYLVRHPAELIPLLMPD

Sequences:

>Translated_221_residues
MMTGIDAVFFDLDGTLADTAPDLAAAANRLVVEHGGSPVAYEKLRPVASHGARGLLGAAFGKHPDDPDFPALRDLFLDYY
EADIAVHTRLFDGMPQVLDALEGAGIRWGIVTNKIARFTVPLVTAIGLAPRASAVVSGDTTPHAKPHPAPLLRAAELSGV
SPKRCVYVGDDLRDIQAGKAAGMLTVTAAYGYCGEGEPPETWGADYLVRHPAELIPLLMPD
>Mature_221_residues
MMTGIDAVFFDLDGTLADTAPDLAAAANRLVVEHGGSPVAYEKLRPVASHGARGLLGAAFGKHPDDPDFPALRDLFLDYY
EADIAVHTRLFDGMPQVLDALEGAGIRWGIVTNKIARFTVPLVTAIGLAPRASAVVSGDTTPHAKPHPAPLLRAAELSGV
SPKRCVYVGDDLRDIQAGKAAGMLTVTAAYGYCGEGEPPETWGADYLVRHPAELIPLLMPD

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=235, Percent_Identity=37.8723404255319, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23350; Mature: 23350

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTGIDAVFFDLDGTLADTAPDLAAAANRLVVEHGGSPVAYEKLRPVASHGARGLLGAAF
CCCCCEEEEEECCCCCCCCCCHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHH
GKHPDDPDFPALRDLFLDYYEADIAVHTRLFDGMPQVLDALEGAGIRWGIVTNKIARFTV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEHHHHHHHHHHH
PLVTAIGLAPRASAVVSGDTTPHAKPHPAPLLRAAELSGVSPKRCVYVGDDLRDIQAGKA
HHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHCCCC
AGMLTVTAAYGYCGEGEPPETWGADYLVRHPAELIPLLMPD
CCEEEEEEECCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCC
>Mature Secondary Structure
MMTGIDAVFFDLDGTLADTAPDLAAAANRLVVEHGGSPVAYEKLRPVASHGARGLLGAAF
CCCCCEEEEEECCCCCCCCCCHHHHHHCEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHH
GKHPDDPDFPALRDLFLDYYEADIAVHTRLFDGMPQVLDALEGAGIRWGIVTNKIARFTV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEHHHHHHHHHHH
PLVTAIGLAPRASAVVSGDTTPHAKPHPAPLLRAAELSGVSPKRCVYVGDDLRDIQAGKA
HHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHCCCC
AGMLTVTAAYGYCGEGEPPETWGADYLVRHPAELIPLLMPD
CCEEEEEEECCCCCCCCCCCCCCCCHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]