Click here to switch to the map view.

The map label for this gene is dosC [H]

Identifier: 73542148

GI number: 73542148

Start: 2703151

End: 2704620

Strand: Direct

Name: dosC [H]

Synonym: Reut_A2462

Alternate gene names: 73542148

Gene position: 2703151-2704620 (Clockwise)

Preceding gene: 73542146

Following gene: 73542152

Centisome position: 71.01

GC content: 64.29

Gene sequence:

>1470_bases
TTGCGCAGTGCAACCGCCGCGTCGTGCGTCGACGATGACTACCAAACCCTGTTTCAACTCGCGCCGGTCTCGCTCTGGCT
GGAAGACTTCAGCGCCGTGCGCCAGCACTTCGAGCGGTTGCGCGCCGAAGGCGTGACGGACTTGCGGGCCTACCTGCGGG
CCAATCCGGATGAAGTCGCGCACTGCTCGTCGCTGATCCGCGTGATCGCGGTGAACCGCCGTACGCTCGACCTGTTCCGC
GCGGCCGACCTGGCCGACCTGGTCGCCAATCTCGACACCGTGTTCCGCGACGACATGTTCGACCAGCACGTCGAGGAACT
CGGCCAGCTTTGGGACGGCGGCAACCGCTTCGCAAGCCAGACCGTGAACTACACGCTCGAAGGCGAGCGCCTCGACATCC
GGCTCGAAGCCACCGTCATGCCCGGGCATGAAGCCACGTGGGAACGTGTGCTGCTGTCGATCGAGGACATCACGGCGCGC
GTGCGCACCGAACGCGACCTGCGCCGCAGCGAGCAATACGCGCTGGGGCTATTCGAGCATTCGCCGGTGTCGCTGTGGGT
GGAGGACTTCAGCGTCGTGAAGATGCTGCTCGACGAAGTGCGCGCGGCCGGCATTACGGACTTTCGCACTTTTCTCAACG
TGCATCCGGATTTCGTCTCGCGCTGCATGCAGGAAATCCGCGTGCTCGACGTCAACCAGCAGACGCTGCTGATGTTCGGC
GCCGAATCGAAGGAAATCCTGCTCTCGCGGCTGGGCGACGTGTTTCGCGACGACATGCGCATCCACTTTGCCGAGCAACT
CATCGACCTGTGGCACGAGAAGCTGTGGCAGCAGCGTGAGGTCATCAACTACGCGCTGGACGGTCGTTCTGTCGACGTCT
TCATGCAATGGTCGGTGTTCCCCGGATGCGAGCAGGAGTGGGACCAGGTGCTGGTGTCGCTGACCGACATCACGGCCCGC
AAGAAGGCGGAGGCCTATGTCGAATTCCTGGGCAAGCACGACGTCCTGACCAAGCTGTACAACCGCGCTTTCTACGAAGA
TGAACTGGCCCGCCTGGGCCGCAAGGGCCCGTGGCCGGTCAGCGTGGTTGCCGTGGACCTGAACGGGCTCAAGACGGTCA
ACGACCAGTTCGGCCATGGCGATGGCGACGCCCTGCTGCGGCGCACCGGCGAGGTACTCAAGAAGGCCGTGGGCGAACAG
GTCTGCGTGGCCCGCATTGGCGGCGACGAGTTCATGGTCCTGCTGCCCGGCCGCGACGAACGCGGCGCGGCGACGGTGGT
GGAGCAGATCGAAAAGGTTGTGGAACTGAACAACCAGTTCTACCCGGGAACAGCGCTCAGTTTCTCGATCGGCCATGCCA
CCTGCCACCAGGGCGAACGCCTGAGCGACACGGTCAAGATCGCCGACAGCCGCATGTACGAGGCCAAGCGCGCCCACTAC
GAAGGCCTGGGGGATCGCCGGGGGGATTGA

Upstream 100 bases:

>100_bases
TTTCGTTTGCGCTGCCCTATCCTTGTCCGCTGGCGCGGCTGACGGGGCCGCCGCAGCACCACAGCGATCAACGGGAATAC
AAGAAGAAGGGAGAATGGCC

Downstream 100 bases:

>100_bases
TCGGCAGCCTCATCGCAAAAGAGCCGGCAAATGCCGGCTCTTTTCGTATCCGCACCGGTCTGCCGGTGCTTACAGGTAGA
TGATCAGCGCGATGCCCGCG

Product: PAS:GGDEF

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRSATAASCVDDDYQTLFQLAPVSLWLEDFSAVRQHFERLRAEGVTDLRAYLRANPDEVAHCSSLIRVIAVNRRTLDLFR
AADLADLVANLDTVFRDDMFDQHVEELGQLWDGGNRFASQTVNYTLEGERLDIRLEATVMPGHEATWERVLLSIEDITAR
VRTERDLRRSEQYALGLFEHSPVSLWVEDFSVVKMLLDEVRAAGITDFRTFLNVHPDFVSRCMQEIRVLDVNQQTLLMFG
AESKEILLSRLGDVFRDDMRIHFAEQLIDLWHEKLWQQREVINYALDGRSVDVFMQWSVFPGCEQEWDQVLVSLTDITAR
KKAEAYVEFLGKHDVLTKLYNRAFYEDELARLGRKGPWPVSVVAVDLNGLKTVNDQFGHGDGDALLRRTGEVLKKAVGEQ
VCVARIGGDEFMVLLPGRDERGAATVVEQIEKVVELNNQFYPGTALSFSIGHATCHQGERLSDTVKIADSRMYEAKRAHY
EGLGDRRGD

Sequences:

>Translated_489_residues
MRSATAASCVDDDYQTLFQLAPVSLWLEDFSAVRQHFERLRAEGVTDLRAYLRANPDEVAHCSSLIRVIAVNRRTLDLFR
AADLADLVANLDTVFRDDMFDQHVEELGQLWDGGNRFASQTVNYTLEGERLDIRLEATVMPGHEATWERVLLSIEDITAR
VRTERDLRRSEQYALGLFEHSPVSLWVEDFSVVKMLLDEVRAAGITDFRTFLNVHPDFVSRCMQEIRVLDVNQQTLLMFG
AESKEILLSRLGDVFRDDMRIHFAEQLIDLWHEKLWQQREVINYALDGRSVDVFMQWSVFPGCEQEWDQVLVSLTDITAR
KKAEAYVEFLGKHDVLTKLYNRAFYEDELARLGRKGPWPVSVVAVDLNGLKTVNDQFGHGDGDALLRRTGEVLKKAVGEQ
VCVARIGGDEFMVLLPGRDERGAATVVEQIEKVVELNNQFYPGTALSFSIGHATCHQGERLSDTVKIADSRMYEAKRAHY
EGLGDRRGD
>Mature_489_residues
MRSATAASCVDDDYQTLFQLAPVSLWLEDFSAVRQHFERLRAEGVTDLRAYLRANPDEVAHCSSLIRVIAVNRRTLDLFR
AADLADLVANLDTVFRDDMFDQHVEELGQLWDGGNRFASQTVNYTLEGERLDIRLEATVMPGHEATWERVLLSIEDITAR
VRTERDLRRSEQYALGLFEHSPVSLWVEDFSVVKMLLDEVRAAGITDFRTFLNVHPDFVSRCMQEIRVLDVNQQTLLMFG
AESKEILLSRLGDVFRDDMRIHFAEQLIDLWHEKLWQQREVINYALDGRSVDVFMQWSVFPGCEQEWDQVLVSLTDITAR
KKAEAYVEFLGKHDVLTKLYNRAFYEDELARLGRKGPWPVSVVAVDLNGLKTVNDQFGHGDGDALLRRTGEVLKKAVGEQ
VCVARIGGDEFMVLLPGRDERGAATVVEQIEKVVELNNQFYPGTALSFSIGHATCHQGERLSDTVKIADSRMYEAKRAHY
EGLGDRRGD

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=151, Percent_Identity=36.4238410596026, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082007, Length=154, Percent_Identity=35.7142857142857, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081974, Length=148, Percent_Identity=31.0810810810811, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=28.7581699346405, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=30.2469135802469, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787541, Length=195, Percent_Identity=29.2307692307692, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=29.3103448275862, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787802, Length=104, Percent_Identity=33.6538461538462, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 55747; Mature: 55747

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSATAASCVDDDYQTLFQLAPVSLWLEDFSAVRQHFERLRAEGVTDLRAYLRANPDEVA
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHH
HCSSLIRVIAVNRRTLDLFRAADLADLVANLDTVFRDDMFDQHVEELGQLWDGGNRFASQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVNYTLEGERLDIRLEATVMPGHEATWERVLLSIEDITARVRTERDLRRSEQYALGLFEH
HEEEEEECCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
SPVSLWVEDFSVVKMLLDEVRAAGITDFRTFLNVHPDFVSRCMQEIRVLDVNQQTLLMFG
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHEECCCCCEEEEEC
AESKEILLSRLGDVFRDDMRIHFAEQLIDLWHEKLWQQREVINYALDGRSVDVFMQWSVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
PGCEQEWDQVLVSLTDITARKKAEAYVEFLGKHDVLTKLYNRAFYEDELARLGRKGPWPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
SVVAVDLNGLKTVNDQFGHGDGDALLRRTGEVLKKAVGEQVCVARIGGDEFMVLLPGRDE
EEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
RGAATVVEQIEKVVELNNQFYPGTALSFSIGHATCHQGERLSDTVKIADSRMYEAKRAHY
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EGLGDRRGD
CCCCCCCCC
>Mature Secondary Structure
MRSATAASCVDDDYQTLFQLAPVSLWLEDFSAVRQHFERLRAEGVTDLRAYLRANPDEVA
CCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHH
HCSSLIRVIAVNRRTLDLFRAADLADLVANLDTVFRDDMFDQHVEELGQLWDGGNRFASQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
TVNYTLEGERLDIRLEATVMPGHEATWERVLLSIEDITARVRTERDLRRSEQYALGLFEH
HEEEEEECCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
SPVSLWVEDFSVVKMLLDEVRAAGITDFRTFLNVHPDFVSRCMQEIRVLDVNQQTLLMFG
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHEECCCCCEEEEEC
AESKEILLSRLGDVFRDDMRIHFAEQLIDLWHEKLWQQREVINYALDGRSVDVFMQWSVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
PGCEQEWDQVLVSLTDITARKKAEAYVEFLGKHDVLTKLYNRAFYEDELARLGRKGPWPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
SVVAVDLNGLKTVNDQFGHGDGDALLRRTGEVLKKAVGEQVCVARIGGDEFMVLLPGRDE
EEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCC
RGAATVVEQIEKVVELNNQFYPGTALSFSIGHATCHQGERLSDTVKIADSRMYEAKRAHY
CCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
EGLGDRRGD
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]