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The map label for this gene is nusA [H]

Identifier: 73541717

GI number: 73541717

Start: 2227649

End: 2229124

Strand: Reverse

Name: nusA [H]

Synonym: Reut_A2029

Alternate gene names: 73541717

Gene position: 2229124-2227649 (Counterclockwise)

Preceding gene: 73541718

Following gene: 73541716

Centisome position: 58.56

GC content: 61.86

Gene sequence:

>1476_bases
ATGAGCCGCGAAGTTCTGTTGCTCGTCGATGCGCTTGCGCGTGAGAAGAACGTCGACAAGGATGTGGTATTCGGCGCGCT
GGAGGCTGCACTGGCCTCGGCGACCAAGAAGCGTTTCGAGGAAGACGTGGATATCCGTGTCGCTATCGACCGGGAATCGG
GCGAGCACGAGACGTTCCGCCGCTGGCTTGTTGTCCCTGATGAACAGGGCCTGCAGGAGCCGGACAAGCAGATCCTGCTG
TTCGAGGCGCGCGATGACGACGCCAGCCTCGAGCTGGGCGACTATGTCGAACAGCAGATCGAGTCGGTCGAATTCGGTCG
TATCGGCGCGCAGGCCGCCAAGCAGGTGATCCTGCAGCGTATCCGCGACGCCGAGCGTGAGCAGATCCTGAACGATTACC
TCGAGCGCGGCGAAAAGATCATGACCGGCACGGTCAAGCGTGCCGACAAGAAGGGCCTGATCGTCGAATCCGGTCGCGTG
GAAGCGCTGCTGGCACGCGACCAGATCATCCCGAAGGAAAACCTGCGCACCGGCGACCGTGTGCGTGCGTACATCCTGAA
TGTGGATCGCGCCGCGCGCGGCCCGCAGATCGAACTGTCGCGCACCGCGCCCGAGTTCCTGATCAAGCTCTTCGAGAACG
AAGTGCCGGAGATGGAGCAGGGCTTGCTCGAGATCAAGGCGGCTGCCCGTGACCCGGGCGTGCGTGCCAAGATCGCCGTC
GTGGCGCATGACAAGCGTATCGATCCGATCGGTACCTGCGTCGGCGTGCGCGGTACGCGGGTGACGGCCGTGCGCAACGA
GATCGGCGGCGAAGCCGTGGACATCGTGCTCTGGTCCGAAGATCCGGCGCAGTTCGTGATCGGCGCGCTGGCGCCGGCAC
AGGTACAGTCGATCGTCGTGGACGAGGAAAAGCACTGCATGGATGTGGTCGTCGATGAGGAGAACCTCGCGGTGGCCATC
GGTCGCAGTGGTCAGAACGTACGCCTGGCGTCCGAGCTGACCGGCTGGCAGATCAACATCATGACGCAGGAAGAGTCGGC
GCAGAAACAGGCCGAGGAAAGCGATGCCGTGCGCAAGCTGTTCATGGCCAAGCTCGACGTCGACGAAGAAGTGGCGGACA
TCCTGATCGAGGAAGGGTTCTCCACGCTGGAAGAAGTGGCCTATGTTCCCATCAGTGAAATGATGGAAATCGAGGCGTTT
GATGAAGACACCGTCAACGAGCTGCGCAACCGCGCACGTGATGCGCTCCTGACGATGGAACTGGCCCGGGAAGAGAAGGT
GGAAGAGGTGTCGCAGGATCTGCGCTCGCTCGACGGCCTGAATCCCGAGCTGATCGGCAAGTTGGCCGAAGGTGATATCC
ATACGCGTGACGACCTGGCCGAACTGGCCGTCGACGAACTCGTCGAGATGACGGGCGTCACCGAGGAAGAAGCCAAGGCG
CTGATCATGAAAGCACGGGAACATTGGTTTAACTGA

Upstream 100 bases:

>100_bases
ATGGTCCGGCGTTGCTGGAATTCACTGTATCTGATGTCGACAAGGCGCGCCTGGTGCCGGTGATCGACTTCAAAGGAAAT
CAAAGAAAAGGGAACAAGCA

Downstream 100 bases:

>100_bases
GGGACACGTCCGGGACGCGTGCACAGCACATTGTCCGGACGCGCTTTCCGCATACGATTTGTCCCGACGTAGAAACCATG
CATTGAAAGGGTTTGAATGG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVAIDRESGEHETFRRWLVVPDEQGLQEPDKQILL
FEARDDDASLELGDYVEQQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTVKRADKKGLIVESGRV
EALLARDQIIPKENLRTGDRVRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
VAHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHCMDVVVDEENLAVAI
GRSGQNVRLASELTGWQINIMTQEESAQKQAEESDAVRKLFMAKLDVDEEVADILIEEGFSTLEEVAYVPISEMMEIEAF
DEDTVNELRNRARDALLTMELAREEKVEEVSQDLRSLDGLNPELIGKLAEGDIHTRDDLAELAVDELVEMTGVTEEEAKA
LIMKAREHWFN

Sequences:

>Translated_491_residues
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVAIDRESGEHETFRRWLVVPDEQGLQEPDKQILL
FEARDDDASLELGDYVEQQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTVKRADKKGLIVESGRV
EALLARDQIIPKENLRTGDRVRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
VAHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHCMDVVVDEENLAVAI
GRSGQNVRLASELTGWQINIMTQEESAQKQAEESDAVRKLFMAKLDVDEEVADILIEEGFSTLEEVAYVPISEMMEIEAF
DEDTVNELRNRARDALLTMELAREEKVEEVSQDLRSLDGLNPELIGKLAEGDIHTRDDLAELAVDELVEMTGVTEEEAKA
LIMKAREHWFN
>Mature_490_residues
SREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVAIDRESGEHETFRRWLVVPDEQGLQEPDKQILLF
EARDDDASLELGDYVEQQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLERGEKIMTGTVKRADKKGLIVESGRVE
ALLARDQIIPKENLRTGDRVRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAVV
AHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHCMDVVVDEENLAVAIG
RSGQNVRLASELTGWQINIMTQEESAQKQAEESDAVRKLFMAKLDVDEEVADILIEEGFSTLEEVAYVPISEMMEIEAFD
EDTVNELRNRARDALLTMELAREEKVEEVSQDLRSLDGLNPELIGKLAEGDIHTRDDLAELAVDELVEMTGVTEEEAKAL
IMKAREHWFN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=52.2267206477733, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54955; Mature: 54824

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVAIDRESGEHETFR
CCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RWLVVPDEQGLQEPDKQILLFEARDDDASLELGDYVEQQIESVEFGRIGAQAAKQVILQR
EEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDAEREQILNDYLERGEKIMTGTVKRADKKGLIVESGRVEALLARDQIIPKENLRTGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCEEHHHHCCCCCCCCCCCCC
VRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
EEEEEEECHHCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VAHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVV
EEECCCCCHHHHEECCCCCEEEHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHC
DEEKHCMDVVVDEENLAVAIGRSGQNVRLASELTGWQINIMTQEESAQKQAEESDAVRKL
CCCCCCEEEEECCCCEEEEECCCCCCEEEHCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
FMAKLDVDEEVADILIEEGFSTLEEVAYVPISEMMEIEAFDEDTVNELRNRARDALLTME
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAREEKVEEVSQDLRSLDGLNPELIGKLAEGDIHTRDDLAELAVDELVEMTGVTEEEAKA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
LIMKAREHWFN
HHHHHHHHCCC
>Mature Secondary Structure 
SREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVAIDRESGEHETFR
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
RWLVVPDEQGLQEPDKQILLFEARDDDASLELGDYVEQQIESVEFGRIGAQAAKQVILQR
EEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRDAEREQILNDYLERGEKIMTGTVKRADKKGLIVESGRVEALLARDQIIPKENLRTGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCEEHHHHCCCCCCCCCCCCC
VRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAV
EEEEEEECHHCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEE
VAHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVV
EEECCCCCHHHHEECCCCCEEEHHHHHCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHC
DEEKHCMDVVVDEENLAVAIGRSGQNVRLASELTGWQINIMTQEESAQKQAEESDAVRKL
CCCCCCEEEEECCCCEEEEECCCCCCEEEHCCCCCEEEEEEECHHHHHHHHHHHHHHHHH
FMAKLDVDEEVADILIEEGFSTLEEVAYVPISEMMEIEAFDEDTVNELRNRARDALLTME
HHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LAREEKVEEVSQDLRSLDGLNPELIGKLAEGDIHTRDDLAELAVDELVEMTGVTEEEAKA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
LIMKAREHWFN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]