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The map label for this gene is rbfA [H]

Identifier: 73541715

GI number: 73541715

Start: 2224221

End: 2224589

Strand: Reverse

Name: rbfA [H]

Synonym: Reut_A2027

Alternate gene names: 73541715

Gene position: 2224589-2224221 (Counterclockwise)

Preceding gene: 73541716

Following gene: 73541714

Centisome position: 58.44

GC content: 59.08

Gene sequence:

>369_bases
ATGGCCAAGAAAGGCAATATCTCCTCCCGCAATCTGCGCATTTCCGACCAGATTCAGAAAGAACTGGCCGAAATGATCCA
GCGCGAAATCCGTGATCCGCGCCTGGGGCTCGTGACGCTCCAGTCCGTGGCGCTGACGCCGGACTACGCGCACGCCAAGG
TGTATTTCACCGTGCTCGGTGCGGAACCCGAAGTGGCCGCTGCCATTCTCAAGGAGAAGGCTGGCTACCTGCACTCGCTG
CTGTTCAAGCGCCTGCACATCCATACGGTGCCGACGCTGCATTTCCATCACGACACGTCGGTGGAGCATGCGATCGAGAT
GTCCAAGCTGATCAACGAAGCGAACGCCACGCGTTCCAAGGACGACTGA

Upstream 100 bases:

>100_bases
AAGTGTACGAAATCACCGAGGTGGCGCGTACGCTGTAAGGCGATACGCAAAACGGCAGGCTCCGGCCTGCCGTTTTCACT
TTGGAACAAGACAGTATTGA

Downstream 100 bases:

>100_bases
TTCCGGCCTCGCCGGGAAGCGCCGTCCTGGCGCGGGCCGTATCGCCCGTCGCCCCGCATTGTTTCCATGACTGACTCCGC
AAACAACCGTCCGCCCCGGC

Product: ribosome-binding factor A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MAKKGNISSRNLRISDQIQKELAEMIQREIRDPRLGLVTLQSVALTPDYAHAKVYFTVLGAEPEVAAAILKEKAGYLHSL
LFKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRSKDD

Sequences:

>Translated_122_residues
MAKKGNISSRNLRISDQIQKELAEMIQREIRDPRLGLVTLQSVALTPDYAHAKVYFTVLGAEPEVAAAILKEKAGYLHSL
LFKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRSKDD
>Mature_121_residues
AKKGNISSRNLRISDQIQKELAEMIQREIRDPRLGLVTLQSVALTPDYAHAKVYFTVLGAEPEVAAAILKEKAGYLHSLL
FKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRSKDD

Specific function: Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Essential for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA [H]

COG id: COG0858

COG function: function code J; Ribosome-binding factor A

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rbfA family [H]

Homologues:

Organism=Escherichia coli, GI1789558, Length=108, Percent_Identity=41.6666666666667, Blast_Score=85, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015946
- InterPro:   IPR000238
- ProDom:   PD007327 [H]

Pfam domain/function: PF02033 RBFA [H]

EC number: NA

Molecular weight: Translated: 13750; Mature: 13619

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKGNISSRNLRISDQIQKELAEMIQREIRDPRLGLVTLQSVALTPDYAHAKVYFTVLG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEEEEC
AEPEVAAAILKEKAGYLHSLLFKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCC
DD
CC
>Mature Secondary Structure 
AKKGNISSRNLRISDQIQKELAEMIQREIRDPRLGLVTLQSVALTPDYAHAKVYFTVLG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCEEEEEEEEC
AEPEVAAAILKEKAGYLHSLLFKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRSK
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCC
DD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA