Click here to switch to the map view.

The map label for this gene is truB

Identifier: 73541714

GI number: 73541714

Start: 2223207

End: 2224154

Strand: Reverse

Name: truB

Synonym: Reut_A2026

Alternate gene names: 73541714

Gene position: 2224154-2223207 (Counterclockwise)

Preceding gene: 73541715

Following gene: 73541709

Centisome position: 58.43

GC content: 67.3

Gene sequence:

>948_bases
ATGACTGACTCCGCAAACAACCGTCCGCCCCGGCTCCCGCGCCGCGAGGTGCACGGCGTGCTGTTGCTCGACAAGCCGCT
AGGGCTGTCTTCCAACGACGCGCTCGTGCGCGCCAAACGCCTGCTGCGGGCCCTCAAGGCCGGCCACACCGGCACGTTGG
ACCCTCTCGCCACAGGGCTGCTGCCGCTTTGCTTTGGCGAGGCGACGAAGTTCTCGCAGGACTTGCTCGATGCGGACAAG
ACTTACGAGGCGGTCGTCAGGCTCGGTCAGAAAACCACTACCGGCGATGCCGAAGGCGAAGTTGTGGTTGAGCGGCCGGT
GAGCTGCGACCGTGCCGGGCTGGACGCCGCAATCGCACGTTTCCTCGGTGAGATCGACCAGGTTCCGCCGATGCATTCTG
CGCTGAAGAAGGATGGCCGGCCGCTGTATGAGTACGCGCGAGCGGGACAGACCGTGGAGCGACCCGCCCGGCGCGTCACG
ATTCACGCGATCGACGTGCTCGATTGTGCCTTGCCGCTGGCGCCTTCCTTCACGATGCGGGTGAAGTGCAGCAAGGGTAC
GTATATTCGCACGCTGGCCGAGGATATTGGCGAGGCGCTCGGCTGTGGCGCACACCTGACCGGGTTGCGCCGCATCGCTG
TCGGCGACCTGACGCTCGATGGCGCTGTGACGCTCGATCAGATCGACGCGCAGGCGGACGAGGCCCGCCCGGCCATGCTG
GCGCCGGTCGATGCGCTGTTGCGACGCTGCCCGCCGGTGACGCTGTCCGCCGAAGCGATGTCGCGCTTCCTGCAAGGTCA
ACGCCTCGCATACCGGGATCTGGACCCCGACAGTGCGCCGCAGGAAGGCGTGCTTGCGCGTGTCTATGGCGGCGAGCCGC
CGAAGCTGCTCGGCGTGGCCCGCATGCGTGAAGGCGCCCTGCGGCCGGAACGACTGGTCCGGCTGTAA

Upstream 100 bases:

>100_bases
CGAAGCGAACGCCACGCGTTCCAAGGACGACTGATTCCGGCCTCGCCGGGAAGCGCCGTCCTGGCGCGGGCCGTATCGCC
CGTCGCCCCGCATTGTTTCC

Downstream 100 bases:

>100_bases
GCGTCCGGCAGGCCGCTCCCACGCAGACATTGCAACAGGCGGAAGGTGTTGCCGCCGCCGACTACGATCAGCTCGGCGCT
GTCGATGATCCGGCGTGCCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTDSANNRPPRLPRREVHGVLLLDKPLGLSSNDALVRAKRLLRALKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYEAVVRLGQKTTTGDAEGEVVVERPVSCDRAGLDAAIARFLGEIDQVPPMHSALKKDGRPLYEYARAGQTVERPARRVT
IHAIDVLDCALPLAPSFTMRVKCSKGTYIRTLAEDIGEALGCGAHLTGLRRIAVGDLTLDGAVTLDQIDAQADEARPAML
APVDALLRRCPPVTLSAEAMSRFLQGQRLAYRDLDPDSAPQEGVLARVYGGEPPKLLGVARMREGALRPERLVRL

Sequences:

>Translated_315_residues
MTDSANNRPPRLPRREVHGVLLLDKPLGLSSNDALVRAKRLLRALKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADK
TYEAVVRLGQKTTTGDAEGEVVVERPVSCDRAGLDAAIARFLGEIDQVPPMHSALKKDGRPLYEYARAGQTVERPARRVT
IHAIDVLDCALPLAPSFTMRVKCSKGTYIRTLAEDIGEALGCGAHLTGLRRIAVGDLTLDGAVTLDQIDAQADEARPAML
APVDALLRRCPPVTLSAEAMSRFLQGQRLAYRDLDPDSAPQEGVLARVYGGEPPKLLGVARMREGALRPERLVRL
>Mature_314_residues
TDSANNRPPRLPRREVHGVLLLDKPLGLSSNDALVRAKRLLRALKAGHTGTLDPLATGLLPLCFGEATKFSQDLLDADKT
YEAVVRLGQKTTTGDAEGEVVVERPVSCDRAGLDAAIARFLGEIDQVPPMHSALKKDGRPLYEYARAGQTVERPARRVTI
HAIDVLDCALPLAPSFTMRVKCSKGTYIRTLAEDIGEALGCGAHLTGLRRIAVGDLTLDGAVTLDQIDAQADEARPAMLA
PVDALLRRCPPVTLSAEAMSRFLQGQRLAYRDLDPDSAPQEGVLARVYGGEPPKLLGVARMREGALRPERLVRL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=260, Percent_Identity=35, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI215599015, Length=218, Percent_Identity=32.5688073394495, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4503337, Length=218, Percent_Identity=32.5688073394495, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI2367200, Length=315, Percent_Identity=46.031746031746, Blast_Score=248, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17553978, Length=263, Percent_Identity=28.1368821292776, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323204, Length=262, Percent_Identity=30.1526717557252, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_CUPPJ (Q46ZP3)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296234.1
- ProteinModelPortal:   Q46ZP3
- SMR:   Q46ZP3
- GeneID:   3611883
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2026
- NMPDR:   fig|264198.3.peg.2805
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01851
- BioCyc:   REUT264198:REUT_A2026-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34034; Mature: 33903

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSANNRPPRLPRREVHGVLLLDKPLGLSSNDALVRAKRLLRALKAGHTGTLDPLATGL
CCCCCCCCCCCCCHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYEAVVRLGQKTTTGDAEGEVVVERPVSCDRAGLDAAIAR
HHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
FLGEIDQVPPMHSALKKDGRPLYEYARAGQTVERPARRVTIHAIDVLDCALPLAPSFTMR
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCCHHHHHHHEEEEEHHHHHHHHHCCCCCEEEE
VKCSKGTYIRTLAEDIGEALGCGAHLTGLRRIAVGDLTLDGAVTLDQIDAQADEARPAML
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCEEECCCEEHHHHHCCHHHCCCCHH
APVDALLRRCPPVTLSAEAMSRFLQGQRLAYRDLDPDSAPQEGVLARVYGGEPPKLLGVA
CCHHHHHHHCCCCEECHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHH
RMREGALRPERLVRL
HHHHCCCCHHHHHCC
>Mature Secondary Structure 
TDSANNRPPRLPRREVHGVLLLDKPLGLSSNDALVRAKRLLRALKAGHTGTLDPLATGL
CCCCCCCCCCCCHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LPLCFGEATKFSQDLLDADKTYEAVVRLGQKTTTGDAEGEVVVERPVSCDRAGLDAAIAR
HHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
FLGEIDQVPPMHSALKKDGRPLYEYARAGQTVERPARRVTIHAIDVLDCALPLAPSFTMR
HHHHHHCCCCHHHHHHHCCCHHHHHHHCCCHHHHHHHEEEEEHHHHHHHHHCCCCCEEEE
VKCSKGTYIRTLAEDIGEALGCGAHLTGLRRIAVGDLTLDGAVTLDQIDAQADEARPAML
EEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHEECCEEECCCEEHHHHHCCHHHCCCCHH
APVDALLRRCPPVTLSAEAMSRFLQGQRLAYRDLDPDSAPQEGVLARVYGGEPPKLLGVA
CCHHHHHHHCCCCEECHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEECCCCCHHHHHHH
RMREGALRPERLVRL
HHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA