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The map label for this gene is dnaB [H]

Identifier: 73541690

GI number: 73541690

Start: 2192807

End: 2194198

Strand: Reverse

Name: dnaB [H]

Synonym: Reut_A2002

Alternate gene names: 73541690

Gene position: 2194198-2192807 (Counterclockwise)

Preceding gene: 73541691

Following gene: 73541689

Centisome position: 57.64

GC content: 62.79

Gene sequence:

>1392_bases
ATGAACGCGCCCGTCGCCGACCCCCAACTCGACAGTCTCAAAGTACCCCCGCATTCGATCGAGGCCGAGCAATCGGTACT
CGGTGGTCTGTTGCTGGACAATGCCGCCTGGGACCGTATTGCCGATTTCATCTCCGAAGCGGACTTCTACCGCTTTGACC
ACCGGATGATCTTCCAGAGCATTGCGAAGCTGATCTCCGCCACCAAGCCGGCGGACGTGATCACGGTCTTCGAGATGCTG
CAGGTGGCCGGCAAGGCCGAGGAAGTCGGTGGCCTTGCGTACCTGAACTCGCTCGCGCAGAACACGCCTAGCGCCGCCAA
TATCCGGCGCTACGCGGAAATCGTGCGCGAGCGGGCGGTGCTGCGCAAGCTGGTGACGGTGGCGGATGACATCGCTTCGT
CGGCATTTGCGCCACAGGGCCGTGAAGTCCGCGAACTGCTGGACGCAGCAGAATCCAAGGTCTTCGCGATTGCCGAAGAG
GGCTCGCGCGGCCAGAAGGGTTTCCAGGAAATCCAGCCGCTGCTGACGCAGGTGGTCGAGCGTATCGACGAGCTGTACCA
CCGCGATTCGACTACCGACGTGACCGGCGTGCCGACCGGCTTCATCGACCTCGATAAGATGACCAGCGGCATGCAGCCCG
GTGATCTGGTCATCGTGGCAGGCCGCCCGTCGATGGGCAAGACGGCCTTCTCGCTGAATATCGGCGAACACGTGGCCGTG
GAACAGGGCCTTCCGGTCGCGGTGTTCTCGATGGAAATGGCGGGCACCCAGCTGGCCATGCGTATGCTGGGTTCGGTCGG
TCGCCTGGACCAGCACCGCCTGCGTACCGGCCGGCTGCTCGACGAAGACTGGCCGCGCCTGACGCATGCGATCCAGCGCA
TGAACGACGCCCAGCTCTTCATCGACGAGACGCCCGCGCTGAATCCGATGGAACTGCGTGCGCGCTCGCGCCGGCTGGCA
CGCCAGTGCGGGCAGCTCGGCCTGATCATCATCGATTACCTGCAGCTGATGTCGGGCTCCGGTGGCGGTGAGAACCGGGC
AACCGAAATCTCGGAGATTTCCCGTTCGCTGAAGGGCCTGGCCAAGGAACTGAACTGCCCGGTGATCGCGCTGTCGCAGC
TGAACCGAAGCCTGGAGCAGCGCCCGAACAAGCGCCCGGTGATGTCCGACCTGCGTGAATCCGGCGCTATCGAACAGGAT
GCCGACGTGATCCTGTTCATTTACCGCGACGAAGTCTACAACCAGGATTCGCAGGACAAGGGCACGGCCGAAATCATTAT
CGGCAAGCAGCGTAACGGTCCGATCGGCACGGTGCGCCTGACCTTCCTGGGCCAGTTCACCAAGTTCGATAACTTCAGTG
GCGGTCCTGCCTTCTTCGACAACGACAACTGA

Upstream 100 bases:

>100_bases
CGTTTTCGCTGCACACGTTCTGGTGTCAAGGCCTGATGCCGAATTGTTGCCGCTCATTTGTCAGGCGGGTAGCGTCCGCC
CGCCGGTATAATCCCACCCC

Downstream 100 bases:

>100_bases
AGTCAAGCCTGCCTGCGAGCAAGCGTCTCGTTTGCGACATATGGGCGGGCATTGCAGCAACAACCCTGTAAAATATTGCG
CTTTGATGACGGGGGCTCAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNAPVADPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFISEADFYRFDHRMIFQSIAKLISATKPADVITVFEML
QVAGKAEEVGGLAYLNSLAQNTPSAANIRRYAEIVRERAVLRKLVTVADDIASSAFAPQGREVRELLDAAESKVFAIAEE
GSRGQKGFQEIQPLLTQVVERIDELYHRDSTTDVTGVPTGFIDLDKMTSGMQPGDLVIVAGRPSMGKTAFSLNIGEHVAV
EQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQRMNDAQLFIDETPALNPMELRARSRRLA
RQCGQLGLIIIDYLQLMSGSGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQD
ADVILFIYRDEVYNQDSQDKGTAEIIIGKQRNGPIGTVRLTFLGQFTKFDNFSGGPAFFDNDN

Sequences:

>Translated_463_residues
MNAPVADPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFISEADFYRFDHRMIFQSIAKLISATKPADVITVFEML
QVAGKAEEVGGLAYLNSLAQNTPSAANIRRYAEIVRERAVLRKLVTVADDIASSAFAPQGREVRELLDAAESKVFAIAEE
GSRGQKGFQEIQPLLTQVVERIDELYHRDSTTDVTGVPTGFIDLDKMTSGMQPGDLVIVAGRPSMGKTAFSLNIGEHVAV
EQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQRMNDAQLFIDETPALNPMELRARSRRLA
RQCGQLGLIIIDYLQLMSGSGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQD
ADVILFIYRDEVYNQDSQDKGTAEIIIGKQRNGPIGTVRLTFLGQFTKFDNFSGGPAFFDNDN
>Mature_463_residues
MNAPVADPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFISEADFYRFDHRMIFQSIAKLISATKPADVITVFEML
QVAGKAEEVGGLAYLNSLAQNTPSAANIRRYAEIVRERAVLRKLVTVADDIASSAFAPQGREVRELLDAAESKVFAIAEE
GSRGQKGFQEIQPLLTQVVERIDELYHRDSTTDVTGVPTGFIDLDKMTSGMQPGDLVIVAGRPSMGKTAFSLNIGEHVAV
EQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQRMNDAQLFIDETPALNPMELRARSRRLA
RQCGQLGLIIIDYLQLMSGSGGGENRATEISEISRSLKGLAKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQD
ADVILFIYRDEVYNQDSQDKGTAEIIIGKQRNGPIGTVRLTFLGQFTKFDNFSGGPAFFDNDN

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=455, Percent_Identity=56.7032967032967, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51001; Mature: 51001

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPVADPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFISEADFYRFDHRMIFQS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLISATKPADVITVFEMLQVAGKAEEVGGLAYLNSLAQNTPSAANIRRYAEIVRERAV
HHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRKLVTVADDIASSAFAPQGREVRELLDAAESKVFAIAEEGSRGQKGFQEIQPLLTQVVE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
RIDELYHRDSTTDVTGVPTGFIDLDKMTSGMQPGDLVIVAGRPSMGKTAFSLNIGEHVAV
HHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCHHHH
EQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQRMNDAQLF
HCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEE
IDETPALNPMELRARSRRLARQCGQLGLIIIDYLQLMSGSGGGENRATEISEISRSLKGL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNQDSQDK
HHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCC
GTAEIIIGKQRNGPIGTVRLTFLGQFTKFDNFSGGPAFFDNDN
CCEEEEEECCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNAPVADPQLDSLKVPPHSIEAEQSVLGGLLLDNAAWDRIADFISEADFYRFDHRMIFQS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKLISATKPADVITVFEMLQVAGKAEEVGGLAYLNSLAQNTPSAANIRRYAEIVRERAV
HHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LRKLVTVADDIASSAFAPQGREVRELLDAAESKVFAIAEEGSRGQKGFQEIQPLLTQVVE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
RIDELYHRDSTTDVTGVPTGFIDLDKMTSGMQPGDLVIVAGRPSMGKTAFSLNIGEHVAV
HHHHHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCHHHH
EQGLPVAVFSMEMAGTQLAMRMLGSVGRLDQHRLRTGRLLDEDWPRLTHAIQRMNDAQLF
HCCCCEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEE
IDETPALNPMELRARSRRLARQCGQLGLIIIDYLQLMSGSGGGENRATEISEISRSLKGL
EECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
AKELNCPVIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNQDSQDK
HHHCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCC
GTAEIIIGKQRNGPIGTVRLTFLGQFTKFDNFSGGPAFFDNDN
CCEEEEEECCCCCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]