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The map label for this gene is cysW [H]

Identifier: 73541657

GI number: 73541657

Start: 2155743

End: 2156666

Strand: Reverse

Name: cysW [H]

Synonym: Reut_A1969

Alternate gene names: 73541657

Gene position: 2156666-2155743 (Counterclockwise)

Preceding gene: 73541658

Following gene: 73541656

Centisome position: 56.66

GC content: 63.2

Gene sequence:

>924_bases
ATGGCCGGCGCAGTTTCGGGCCGCCTTGGGCGGCAGGGTGGGGCCCACCACAAGTTCGACGCCACCGGCGAAGCCGTATG
GGTGCGCTTCACGCTGATCACCGTGGCGGTGTTGTTCCTTACGCTGTTCCTGTTCGTGCCGCTCGCATCGGTCTTCTACG
AGGCGCTGCGCAAGGGCGTAGAGACTTACTGGGAAGCGCTGATCGAGCCGGACGCGCTGTCTGCAATCGAGCTCACGCTG
ACGGTGGCCACGATTGCCGTGCCGCTGAACTTGGTGTTCGGCGTGGCTGCAGCATGGGCCATCGCCAAGTTCGAGTTCCC
GGGCAAGAGCCTGCTGACCACGCTGATCGACCTGCCGTTCTCGGTGTCGCCGGTGATCTCGGGCCTGGTCTATGTGCTGA
TGTTCGGCGCGCAGGGCTGGTTTGGTCCGTGGCTGGAGGCGCATGACATCAAGATCATGTTTGCGGTGCCCGGCATCGTG
CTGGCCACGATCTTCGTAACCTTCCCGTTCGTGGCGCGCGAGCTGATTCCGTTGATGCAGGCGCAGGGCAGTGAGGAGGA
AGAAGCCGCCATCGTGCTGGGTGCGTCGGGCTGGCAGACCTTCTGGCACATCACGCTGCCGAATATCCGCTGGGGCCTGC
TGTACGGCGTGATTCTGTGTAACGCCCGTGCGATGGGCGAGTTCGGCGCGGTATCGGTCGTATCGGGCCATATTCGTGGC
CTGACCAACACCATGCCGCTGCACGTGGAGATCCTCTACAACGAGTACAACTTCGCTGCGGCATTCGCGGTCGCATCGCT
GCTGACGCTGCTGGCCCTGGTCACGCTGGGCATCAAGACGCTGGTCGAACTGCGCGCCCGCAAGGAAAACGAAGACGCTG
CCACGGAACTTCCGCTGGCTGCATTGCAAGGACAGGCATCATGA

Upstream 100 bases:

>100_bases
CTCGTGATCAACCTGCTGCAGGCCTGGACGCGCCGCCACCAGTCGGGCGCGCATAGCGGCCTGGCACTCGAAGCGCCCGC
CACGGACAAGGAGCACTGAC

Downstream 100 bases:

>100_bases
GCATTCAGGTCAAGAACGTAGAGAAGCGCTTTGGCGATTTTGTCGCGCTGGACAACGTCTCGCTGGACTTCGAAGAGGGC
GAGCTGACCGCGCTGCTCGG

Product: sulphate transport system permease protein 2:Sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MAGAVSGRLGRQGGAHHKFDATGEAVWVRFTLITVAVLFLTLFLFVPLASVFYEALRKGVETYWEALIEPDALSAIELTL
TVATIAVPLNLVFGVAAAWAIAKFEFPGKSLLTTLIDLPFSVSPVISGLVYVLMFGAQGWFGPWLEAHDIKIMFAVPGIV
LATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHITLPNIRWGLLYGVILCNARAMGEFGAVSVVSGHIRG
LTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVELRARKENEDAATELPLAALQGQAS

Sequences:

>Translated_307_residues
MAGAVSGRLGRQGGAHHKFDATGEAVWVRFTLITVAVLFLTLFLFVPLASVFYEALRKGVETYWEALIEPDALSAIELTL
TVATIAVPLNLVFGVAAAWAIAKFEFPGKSLLTTLIDLPFSVSPVISGLVYVLMFGAQGWFGPWLEAHDIKIMFAVPGIV
LATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHITLPNIRWGLLYGVILCNARAMGEFGAVSVVSGHIRG
LTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVELRARKENEDAATELPLAALQGQAS
>Mature_306_residues
AGAVSGRLGRQGGAHHKFDATGEAVWVRFTLITVAVLFLTLFLFVPLASVFYEALRKGVETYWEALIEPDALSAIELTLT
VATIAVPLNLVFGVAAAWAIAKFEFPGKSLLTTLIDLPFSVSPVISGLVYVLMFGAQGWFGPWLEAHDIKIMFAVPGIVL
ATIFVTFPFVARELIPLMQAQGSEEEEAAIVLGASGWQTFWHITLPNIRWGLLYGVILCNARAMGEFGAVSVVSGHIRGL
TNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVELRARKENEDAATELPLAALQGQAS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=280, Percent_Identity=52.1428571428571, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=38.3495145631068, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786980, Length=229, Percent_Identity=32.7510917030568, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787369, Length=240, Percent_Identity=28.75, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33175; Mature: 33044

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAVSGRLGRQGGAHHKFDATGEAVWVRFTLITVAVLFLTLFLFVPLASVFYEALRKGV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETYWEALIEPDALSAIELTLTVATIAVPLNLVFGVAAAWAIAKFEFPGKSLLTTLIDLPF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SVSPVISGLVYVLMFGAQGWFGPWLEAHDIKIMFAVPGIVLATIFVTFPFVARELIPLMQ
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AQGSEEEEAAIVLGASGWQTFWHITLPNIRWGLLYGVILCNARAMGEFGAVSVVSGHIRG
CCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHC
LTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVELRARKENEDAATELPLA
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHH
ALQGQAS
HHCCCCC
>Mature Secondary Structure 
AGAVSGRLGRQGGAHHKFDATGEAVWVRFTLITVAVLFLTLFLFVPLASVFYEALRKGV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETYWEALIEPDALSAIELTLTVATIAVPLNLVFGVAAAWAIAKFEFPGKSLLTTLIDLPF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
SVSPVISGLVYVLMFGAQGWFGPWLEAHDIKIMFAVPGIVLATIFVTFPFVARELIPLMQ
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
AQGSEEEEAAIVLGASGWQTFWHITLPNIRWGLLYGVILCNARAMGEFGAVSVVSGHIRG
CCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHC
LTNTMPLHVEILYNEYNFAAAFAVASLLTLLALVTLGIKTLVELRARKENEDAATELPLA
CCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHH
ALQGQAS
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]