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The map label for this gene is mls [H]

Identifier: 73541639

GI number: 73541639

Start: 2137180

End: 2138766

Strand: Reverse

Name: mls [H]

Synonym: Reut_A1951

Alternate gene names: 73541639

Gene position: 2138766-2137180 (Counterclockwise)

Preceding gene: 73541640

Following gene: 73541638

Centisome position: 56.19

GC content: 62.51

Gene sequence:

>1587_bases
ATGGCTATCACGCTGCCCGCGGGCATGACGATTACCGGCGAGATCCTGCCGGCCTACGAAGATATCCTCACGCCGGAAGC
CCTGGCCCTGGTAGACAAACTGCACCGCGCCTTCGAGCCGCGCCGTCAGGAACTGCTGGCCGCGCGCGTGGAACGCGCCA
AGCGCCTGGACGCTGGCGAAGTGCCCGATTTCCTGCCGGAAACCAAGCACATCCGCGAAGGCGACTGGAAGGTTGCCCCG
ATCCCCAAGGCCCTGGAATGCCGCCGCGTGGAAATCACCGGTCCGGTCGAGGCCAAGATGGTCATCAACGCCTTCAACTC
GGGCGCTGACAGCTACATGACCGACTTCGAGGACTCCAACACCCCGAACTGGCACAACCAGATGCAGGGTCAGGTGAACC
TGAAGGCTGCCGTGCGCCGCACGCTGACGCTGGAATCGAAGGGCAAGCAATACAAGCTCAACGACAAGATCGCTACGCTG
CAGGTGCGTCCCCGTGGCTGGCACCTCGACGAAAAGCACGTCACGATCGACGGCAAGCGCGTCTCGGGCGGCATCTTCGA
CTTCGCGCTGTTCCTGGTCCACAACGCCAAGGAGCAAATCGCCCGCGGCGACGGCCCGTTCTTCTACCTGCCGAAGATGG
AAAGCCATCTGGAAGCACGCCTGTGGAACGATATCTTCGTGATGGCCCAGAACGAGATCGGCCTGCCGCAAGGCACGATC
AAGGCCACGGTGCTGATCGAAACCATCCTCGCCGCGTTCGAAATGGATGAAATCCTGTACGAACTGCGCGAGCACAGCGC
TGGGCTGAACGCCGGCCGCTGGGACTACATCTTCTCGTGCATCAAGAAGTTCAAGAACGACAAGAACTTCTGCCTGGCCG
ACCGCGCCAAGGTCACCATGACCGCGCCGTTCATGCGCGCGTACGCACTGCTGCTGCTGAAGACCTGCCACTATCGCGGC
GCGCCCGCCATGGGCGGCATGAGCGCACTGATCCCGATCAAGAACGATCCGGAGAAGAACGCCATCGCCATGCAGGGCAT
CATCAACGACAAGAAGCGCGACGCGACCGACGGCTACGACGGCGGCTGGGTGGCTCACCCGGGCCTGGTCGAGCCCGCCA
TGAAGGAATTCGTGGCTGTGCTCGGCGACAAGCCGAACCAGTTCGAGAAGCAGCGTCCGGACGTGAACGTGAAGGGTTCG
GACCTGCTGGACTTCCAGCCGGAAACGCCGATCACCGAAGGCGGCCTGCGCATGAACATCAACGTGGGCATCCACTACCT
GGGCGCCTGGCTGGCCGGCAACGGCTGCGTGCCGATCCACAACCTGATGGAAGATGCGGCCACCGCCGAAATCTCGCGCT
CGCAGGTGTGGCAATGGATCCGCTCGCCGAAGGGCAAGCTGGAAGACGGCACCAAGGTCACGGCCGAACTCGTGCGCAAG
CTGATTCCGGAAGAACTGGCCAAGGTGAAGGCTGAAGTCGGCGGCGAGACCAAGACGTACGACCGCGCTGCGGAAATCTT
CGAGCAGATGTCGACGTCGGAAGACTTTGCCGAATTCCTGACGCTGCCGCTGTACGAAGAAGTCTGA

Upstream 100 bases:

>100_bases
GAACGACCTGCTCGATTTCGTCCGCGCCAAAGGCGTGGAAATCTGAACGGCAACGAGCGGTCAACCCTCTAACAAGCTAT
CGACTAACAGGAGAAGACTG

Downstream 100 bases:

>100_bases
GCCTGTAGCGGCTTAAGGCAGGGCTTAAGGCAGTGAAAAAACCCGCGGTGAGAGCCGCGGGTTTTTGTTTGAGTAGTGGT
ATTGGCGGGCCGGCTGACGA

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MAITLPAGMTITGEILPAYEDILTPEALALVDKLHRAFEPRRQELLAARVERAKRLDAGEVPDFLPETKHIREGDWKVAP
IPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNTPNWHNQMQGQVNLKAAVRRTLTLESKGKQYKLNDKIATL
QVRPRGWHLDEKHVTIDGKRVSGGIFDFALFLVHNAKEQIARGDGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
KATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDKNFCLADRAKVTMTAPFMRAYALLLLKTCHYRG
APAMGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVNVKGS
DLLDFQPETPITEGGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
LIPEELAKVKAEVGGETKTYDRAAEIFEQMSTSEDFAEFLTLPLYEEV

Sequences:

>Translated_528_residues
MAITLPAGMTITGEILPAYEDILTPEALALVDKLHRAFEPRRQELLAARVERAKRLDAGEVPDFLPETKHIREGDWKVAP
IPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNTPNWHNQMQGQVNLKAAVRRTLTLESKGKQYKLNDKIATL
QVRPRGWHLDEKHVTIDGKRVSGGIFDFALFLVHNAKEQIARGDGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
KATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDKNFCLADRAKVTMTAPFMRAYALLLLKTCHYRG
APAMGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVNVKGS
DLLDFQPETPITEGGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
LIPEELAKVKAEVGGETKTYDRAAEIFEQMSTSEDFAEFLTLPLYEEV
>Mature_527_residues
AITLPAGMTITGEILPAYEDILTPEALALVDKLHRAFEPRRQELLAARVERAKRLDAGEVPDFLPETKHIREGDWKVAPI
PKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSNTPNWHNQMQGQVNLKAAVRRTLTLESKGKQYKLNDKIATLQ
VRPRGWHLDEKHVTIDGKRVSGGIFDFALFLVHNAKEQIARGDGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTIK
ATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDKNFCLADRAKVTMTAPFMRAYALLLLKTCHYRGA
PAMGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYDGGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVNVKGSD
LLDFQPETPITEGGLRMNINVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRKL
IPEELAKVKAEVGGETKTYDRAAEIFEQMSTSEDFAEFLTLPLYEEV

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=508, Percent_Identity=49.4094488188976, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=49.4274809160305, Blast_Score=503, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=50.7142857142857, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324212, Length=515, Percent_Identity=50.0970873786408, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6322222, Length=515, Percent_Identity=50.2912621359223, Blast_Score=503, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59105; Mature: 58973

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITLPAGMTITGEILPAYEDILTPEALALVDKLHRAFEPRRQELLAARVERAKRLDAGE
CEEEECCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
VPDFLPETKHIREGDWKVAPIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSN
CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCHHHEEEHHCCCCHHHHHCCCCCC
TPNWHNQMQGQVNLKAAVRRTLTLESKGKQYKLNDKIATLQVRPRGWHLDEKHVTIDGKR
CCCHHHHCCCCEEHHHHHHHHHEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCC
VSGGIFDFALFLVHNAKEQIARGDGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
CCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHH
KATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDKNFCLADRAKVTM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEE
TAPFMRAYALLLLKTCHYRGAPAMGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYD
EHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHCCCCCCCCCCCC
GGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVNVKGSDLLDFQPETPITEGGLRMNI
CCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
NVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
EECCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LIPEELAKVKAEVGGETKTYDRAAEIFEQMSTSEDFAEFLTLPLYEEV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHCC
>Mature Secondary Structure 
AITLPAGMTITGEILPAYEDILTPEALALVDKLHRAFEPRRQELLAARVERAKRLDAGE
EEEECCCCEEECCHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
VPDFLPETKHIREGDWKVAPIPKALECRRVEITGPVEAKMVINAFNSGADSYMTDFEDSN
CCCCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCHHHEEEHHCCCCHHHHHCCCCCC
TPNWHNQMQGQVNLKAAVRRTLTLESKGKQYKLNDKIATLQVRPRGWHLDEKHVTIDGKR
CCCHHHHCCCCEEHHHHHHHHHEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEECCCC
VSGGIFDFALFLVHNAKEQIARGDGPFFYLPKMESHLEARLWNDIFVMAQNEIGLPQGTI
CCCHHHHHHHHHHHCCHHHHHCCCCCEEECCCHHHHHHHHHHCCEEEEEECCCCCCCCHH
KATVLIETILAAFEMDEILYELREHSAGLNAGRWDYIFSCIKKFKNDKNFCLADRAKVTM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEE
TAPFMRAYALLLLKTCHYRGAPAMGGMSALIPIKNDPEKNAIAMQGIINDKKRDATDGYD
EHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCEEEHHHHCCCCCCCCCCCC
GGWVAHPGLVEPAMKEFVAVLGDKPNQFEKQRPDVNVKGSDLLDFQPETPITEGGLRMNI
CCEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
NVGIHYLGAWLAGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGKLEDGTKVTAELVRK
EECCEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LIPEELAKVKAEVGGETKTYDRAAEIFEQMSTSEDFAEFLTLPLYEEV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA