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The map label for this gene is gsiB [H]

Identifier: 73541615

GI number: 73541615

Start: 2111766

End: 2113322

Strand: Reverse

Name: gsiB [H]

Synonym: Reut_A1926

Alternate gene names: 73541615

Gene position: 2113322-2111766 (Counterclockwise)

Preceding gene: 73541616

Following gene: 73541614

Centisome position: 55.52

GC content: 63.07

Gene sequence:

>1557_bases
ATGTCTGCATCGATGGTTTCGCCCAGGCTGGTGGCCGGGCTGCTTGTGGCCGGCGCGATAGGCGCGCTGGCGCCGGTGTC
CGCTTTTGCCGCCAAGGATGCGGTAATGGCTGTCGCTTCCACGTTCACCACGCTGGACCCGTACGATTCGAACGACACGC
TGTCGCAGGCCGTAACCAAAAGCTTCTACCAGGGCCTGTTCGGCCTGGACAAGGACATGAAGCTGGTCAACGTGCTGGCC
GAAAGCTATGACGTCAGCAAGGACGGCCTGACCTACACGATCAAGCTCAAGAAGGGCATCAAGTTCCACGACGGCACCCC
CTTCGACGCCGCCGCCGTGAAGGCCAACCTTGACCGCGTGACCAACCCGGCCAACAAGCTCAAGCGCTATACGCTGTTCA
ACCGCGTCGCCAAGACCGATGTGGTGGACAGCAGCACGGTAAAGGTCACGCTGAAGGAGCCGTTCTCGCCGTTCATCAAC
GTGCTGGCCCACCCGTCGGCCGTGATGATCTCGCCCGTGGCGCTGCAGAAGTACGGCAAGGAGATTGCCTTCCATCCGGT
CGGCACGGGCCCGTTCGAGTTCGTCGAGTGGAAGCAGCCCGATCACCTGAAGGGCAAGAAATTCGCAGGCTACTGGAAGA
CTGGTTATCCGAAGATCGACACCGTCACCTGGAAGCCGGTGGTGGACAACAACACCCGCGCGGCCATCATGCAGACCGGC
GAGGCCGACTTTGCCTTCAGCATCCCGTTCGAGCAGGCCGCGGTGCTGAAGAACAGCCCCAAGGTGGACCTGATCGCGGC
GCCGTCGATTATCCAGCGCTACCTGAGCCTGAACACCACGGTCAAGCCGTTCAACGACCCGAAGGTGCGCCAGGCCATCA
ACTACGCAATCAATAAGGACGCGCTGGCCAAGGTGGCCTTCGCTGGCTACGCCGTGCCGGCCGAAGGCGTGGTGCCGCCG
GGCGTGGACTATGCCGAGAAGCTGGGCCCGTGGCCGTATGACCCGGCCAAGGCGCGCGCGCTGCTGAAGGAAGCCGGCTA
CCCGAACGGCTTCGAGACCACGCTGTGGTCGGCCTATAACCACACCACCGCGCAGAAGGTGATCCAGTTCGTGCAGCAGC
AGCTGCAGCAGGTCGGCATCAAGGCCAGCGTGCAGGCGCTGGAAGCCGGCCAGCGCGTGGAGAAGGTGGAAAGCGTGCAG
AAGCCGGAAGATGCCGGCGTGCGCATCTACTACATCGGCTGGTCGTCGTCGACCGGTGAGTCGGACTGGGCGCTGCGTCC
GCTGCTGGCCTCGGAATCGATGCCGCCGAAGTTGCTGAACACCGCGTACTACAAGAACGACCAGGTCGATGCCGACATCG
CCGGCGCGCTGCGCACGACCGATCGCGGCGAGAAGGCCAAGCTGTACAAGGACGCGCAGGAACGCATCTGGAAGGATGCG
CCGTGGGCCTTCCTGGTGACCGAGAAGGTGCTGTACGCGCGCAGCAAGCGCCTGACCGGTGCGTATGTGATGCCCGATGG
CTCGTTCAATTTCGACGACATCGATATCAAGCAGTAA

Upstream 100 bases:

>100_bases
CACTGGTGCCTGTCGCGGACAGCCGTGGAATCGGTCACTTTGTCGCGCGGCACGCCATTGGCGGTGCCTACTGAAATCGG
TTTATCCAAGGAGAATCAAG

Downstream 100 bases:

>100_bases
GCCTGCGGAAAGCGCGCCGCTACCGCGGCGCGCACCGGGTAGCAAGCAGTAAGCCGAACGTGGCCGCCTGTCCCATCGCA
GGGGCATGGACAGGCGGCCT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSASMVSPRLVAGLLVAGAIGALAPVSAFAAKDAVMAVASTFTTLDPYDSNDTLSQAVTKSFYQGLFGLDKDMKLVNVLA
ESYDVSKDGLTYTIKLKKGIKFHDGTPFDAAAVKANLDRVTNPANKLKRYTLFNRVAKTDVVDSSTVKVTLKEPFSPFIN
VLAHPSAVMISPVALQKYGKEIAFHPVGTGPFEFVEWKQPDHLKGKKFAGYWKTGYPKIDTVTWKPVVDNNTRAAIMQTG
EADFAFSIPFEQAAVLKNSPKVDLIAAPSIIQRYLSLNTTVKPFNDPKVRQAINYAINKDALAKVAFAGYAVPAEGVVPP
GVDYAEKLGPWPYDPAKARALLKEAGYPNGFETTLWSAYNHTTAQKVIQFVQQQLQQVGIKASVQALEAGQRVEKVESVQ
KPEDAGVRIYYIGWSSSTGESDWALRPLLASESMPPKLLNTAYYKNDQVDADIAGALRTTDRGEKAKLYKDAQERIWKDA
PWAFLVTEKVLYARSKRLTGAYVMPDGSFNFDDIDIKQ

Sequences:

>Translated_518_residues
MSASMVSPRLVAGLLVAGAIGALAPVSAFAAKDAVMAVASTFTTLDPYDSNDTLSQAVTKSFYQGLFGLDKDMKLVNVLA
ESYDVSKDGLTYTIKLKKGIKFHDGTPFDAAAVKANLDRVTNPANKLKRYTLFNRVAKTDVVDSSTVKVTLKEPFSPFIN
VLAHPSAVMISPVALQKYGKEIAFHPVGTGPFEFVEWKQPDHLKGKKFAGYWKTGYPKIDTVTWKPVVDNNTRAAIMQTG
EADFAFSIPFEQAAVLKNSPKVDLIAAPSIIQRYLSLNTTVKPFNDPKVRQAINYAINKDALAKVAFAGYAVPAEGVVPP
GVDYAEKLGPWPYDPAKARALLKEAGYPNGFETTLWSAYNHTTAQKVIQFVQQQLQQVGIKASVQALEAGQRVEKVESVQ
KPEDAGVRIYYIGWSSSTGESDWALRPLLASESMPPKLLNTAYYKNDQVDADIAGALRTTDRGEKAKLYKDAQERIWKDA
PWAFLVTEKVLYARSKRLTGAYVMPDGSFNFDDIDIKQ
>Mature_517_residues
SASMVSPRLVAGLLVAGAIGALAPVSAFAAKDAVMAVASTFTTLDPYDSNDTLSQAVTKSFYQGLFGLDKDMKLVNVLAE
SYDVSKDGLTYTIKLKKGIKFHDGTPFDAAAVKANLDRVTNPANKLKRYTLFNRVAKTDVVDSSTVKVTLKEPFSPFINV
LAHPSAVMISPVALQKYGKEIAFHPVGTGPFEFVEWKQPDHLKGKKFAGYWKTGYPKIDTVTWKPVVDNNTRAAIMQTGE
ADFAFSIPFEQAAVLKNSPKVDLIAAPSIIQRYLSLNTTVKPFNDPKVRQAINYAINKDALAKVAFAGYAVPAEGVVPPG
VDYAEKLGPWPYDPAKARALLKEAGYPNGFETTLWSAYNHTTAQKVIQFVQQQLQQVGIKASVQALEAGQRVEKVESVQK
PEDAGVRIYYIGWSSSTGESDWALRPLLASESMPPKLLNTAYYKNDQVDADIAGALRTTDRGEKAKLYKDAQERIWKDAP
WAFLVTEKVLYARSKRLTGAYVMPDGSFNFDDIDIKQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=64.6245059288538, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=30.4255319148936, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1787495, Length=506, Percent_Identity=25.4940711462451, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787762, Length=492, Percent_Identity=26.6260162601626, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789887, Length=459, Percent_Identity=26.5795206971678, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787551, Length=544, Percent_Identity=25.1838235294118, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789397, Length=467, Percent_Identity=22.6980728051392, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56844; Mature: 56713

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASMVSPRLVAGLLVAGAIGALAPVSAFAAKDAVMAVASTFTTLDPYDSNDTLSQAVTK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SFYQGLFGLDKDMKLVNVLAESYDVSKDGLTYTIKLKKGIKFHDGTPFDAAAVKANLDRV
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHH
TNPANKLKRYTLFNRVAKTDVVDSSTVKVTLKEPFSPFINVLAHPSAVMISPVALQKYGK
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCHHEECHHHHHHCCC
EIAFHPVGTGPFEFVEWKQPDHLKGKKFAGYWKTGYPKIDTVTWKPVVDNNTRAAIMQTG
EEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCEEEEECC
EADFAFSIPFEQAAVLKNSPKVDLIAAPSIIQRYLSLNTTVKPFNDPKVRQAINYAINKD
CCCEEEECCHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHH
ALAKVAFAGYAVPAEGVVPPGVDYAEKLGPWPYDPAKARALLKEAGYPNGFETTLWSAYN
HHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHC
HTTAQKVIQFVQQQLQQVGIKASVQALEAGQRVEKVESVQKPEDAGVRIYYIGWSSSTGE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
SDWALRPLLASESMPPKLLNTAYYKNDQVDADIAGALRTTDRGEKAKLYKDAQERIWKDA
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PWAFLVTEKVLYARSKRLTGAYVMPDGSFNFDDIDIKQ
CEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SASMVSPRLVAGLLVAGAIGALAPVSAFAAKDAVMAVASTFTTLDPYDSNDTLSQAVTK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SFYQGLFGLDKDMKLVNVLAESYDVSKDGLTYTIKLKKGIKFHDGTPFDAAAVKANLDRV
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHHHHHHH
TNPANKLKRYTLFNRVAKTDVVDSSTVKVTLKEPFSPFINVLAHPSAVMISPVALQKYGK
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCHHEECHHHHHHCCC
EIAFHPVGTGPFEFVEWKQPDHLKGKKFAGYWKTGYPKIDTVTWKPVVDNNTRAAIMQTG
EEEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCEECCCCCEEEEECC
EADFAFSIPFEQAAVLKNSPKVDLIAAPSIIQRYLSLNTTVKPFNDPKVRQAINYAINKD
CCCEEEECCHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHH
ALAKVAFAGYAVPAEGVVPPGVDYAEKLGPWPYDPAKARALLKEAGYPNGFETTLWSAYN
HHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHC
HTTAQKVIQFVQQQLQQVGIKASVQALEAGQRVEKVESVQKPEDAGVRIYYIGWSSSTGE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
SDWALRPLLASESMPPKLLNTAYYKNDQVDADIAGALRTTDRGEKAKLYKDAQERIWKDA
CCCCCHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
PWAFLVTEKVLYARSKRLTGAYVMPDGSFNFDDIDIKQ
CEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA