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The map label for this gene is aspA [H]

Identifier: 73541480

GI number: 73541480

Start: 1954847

End: 1956274

Strand: Reverse

Name: aspA [H]

Synonym: Reut_A1791

Alternate gene names: 73541480

Gene position: 1956274-1954847 (Counterclockwise)

Preceding gene: 73541481

Following gene: 73541479

Centisome position: 51.39

GC content: 62.61

Gene sequence:

>1428_bases
ATGACTGCCGGACCCTTCAGAACCGAACATGACCTGCTGGGCGACCGGGAAGTCCCGGCCCAGGCTTACTACGGTGTCCA
CACCCTGCGCGCCGTCGAGAACTTCCCGATCACGGGCACGCCAATTTCCATCTACCCGGAACTGATCAAGGCGCTGGCCG
CGATCAAGCAAGCCGCGGCGCAAGCGAACCATGAGCTTGAGCTGCTAGACCAGGCACGCTGCGACGCGATCGTCGCTGCC
TGTCAGGAACTGATGGCCGGAAAGCTGCACGATCAGTTCGTGGTCGACGTGATCCAGGGCGGCGCTGGCACCTCGACAAA
CATGAACGCCAATGAGGTCATCGCCAATCGCGCGCTCGAGCTTCTCGGGCATCAGAAGGGCGAGTACCAGCACCTGCATC
CGAACGAACACGTCAATATCGGCCAGAGCACCAACGATGTCTATCCGACCGCGTTGAAGGTCGCGACATGGTTCGGCATC
CGTGGGCTTGTGGAGGCCATGGCGGTACTGCGCCAGGCGTTCGAGGCGAAGGCCGAGGAGTTCGCCCACATCATCAAGAT
GGGCCGCACGCAATTGCAGGATGCGGTGCCGATGACGCTCGGCCAGGAATTCAGCACCTATGCCGTGATGCTCGGCGAAG
ACGAGGCGCGCTTGCGTGAAGCCGCGATGCTGATCTGCGAGATCAACCTCGGCGCCACCGCCATTGGCACCGGCATTACC
GCGCACCCATCCTATGCGCCGGTGGTGCTGCGGCGGCTGCGCGACATCACTGGCATTCCGCTTGAGACAGCGCCAAACCT
GATCGAAGCTACGCAGGATTGCGGCGCCTTCGTGCAACTGTCCGGCGTGCTAAAACGCGTTGCGGTGAAGCTTTCAAAGA
CCTGCAACGACCTGCGGCTGTTGTCGAGCGGCCCGCGTGCGGGGCTGTGCGAAATCAACCTGCCGCCGATGCAGGCCGGC
TCCAGCATCATGCCCGGCAAGGTGAATCCGGTGATCCCCGAAGTCGTGAACCAGATTGCCTTCGAGGTCATCGGCAACGA
CGTGACGGTCAGCTTCGCAGCCGAAGCCGGCCAGCTGCAGCTCAATGCGTTCGAGCCGATCATTGCCCACAGCCTGTTCA
AGAGCGTCAGCCATCTGCGCAACGGTTGCCTGACGCTCGCCGAGCGTTGCGTCAAGGGCATCACGGCAAATGCAGATCGG
CTGCGGGCTACCGTCGAGAACTCGATCGGGATCGTCACGGCGCTCAACCCTTACATCGGCTATGCGAACGCAACAGCCGT
CGCGCAGGAAGCCCACGCCACCGGCGGCAGCGTCTATGAGATCGTGCTGCGCAAGGGCCTGTTGTCGAAAGAGGAGCTTG
ACCACATCCTGCGGCCCGAGACGCTGACACAGCCCGTGCCGCTCGATCTGCATGGGAAGTCGCACTGA

Upstream 100 bases:

>100_bases
CGCAGGCCATGAAATCTCGGCATTGAACGTAGCGGTGGAGCAGCCGATTCCCTAGACTTCTGAAGGAAAGCTGAAGCCAC
TTTACGCGAGGAGCGCGACC

Downstream 100 bases:

>100_bases
AAGATTGGGGCGGCAACTCTGTCTTCTCATTTTCCGCCACCAGCGGTATTTGCCGCCGCCGGTATACCTCCTCGCGCCGC
ATTCTATTCCCGGATCCGGA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTAGPFRTEHDLLGDREVPAQAYYGVHTLRAVENFPITGTPISIYPELIKALAAIKQAAAQANHELELLDQARCDAIVAA
CQELMAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHQKGEYQHLHPNEHVNIGQSTNDVYPTALKVATWFGI
RGLVEAMAVLRQAFEAKAEEFAHIIKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEARLREAAMLICEINLGATAIGTGIT
AHPSYAPVVLRRLRDITGIPLETAPNLIEATQDCGAFVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLCEINLPPMQAG
SSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPIIAHSLFKSVSHLRNGCLTLAERCVKGITANADR
LRATVENSIGIVTALNPYIGYANATAVAQEAHATGGSVYEIVLRKGLLSKEELDHILRPETLTQPVPLDLHGKSH

Sequences:

>Translated_475_residues
MTAGPFRTEHDLLGDREVPAQAYYGVHTLRAVENFPITGTPISIYPELIKALAAIKQAAAQANHELELLDQARCDAIVAA
CQELMAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHQKGEYQHLHPNEHVNIGQSTNDVYPTALKVATWFGI
RGLVEAMAVLRQAFEAKAEEFAHIIKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEARLREAAMLICEINLGATAIGTGIT
AHPSYAPVVLRRLRDITGIPLETAPNLIEATQDCGAFVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLCEINLPPMQAG
SSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPIIAHSLFKSVSHLRNGCLTLAERCVKGITANADR
LRATVENSIGIVTALNPYIGYANATAVAQEAHATGGSVYEIVLRKGLLSKEELDHILRPETLTQPVPLDLHGKSH
>Mature_474_residues
TAGPFRTEHDLLGDREVPAQAYYGVHTLRAVENFPITGTPISIYPELIKALAAIKQAAAQANHELELLDQARCDAIVAAC
QELMAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALELLGHQKGEYQHLHPNEHVNIGQSTNDVYPTALKVATWFGIR
GLVEAMAVLRQAFEAKAEEFAHIIKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEARLREAAMLICEINLGATAIGTGITA
HPSYAPVVLRRLRDITGIPLETAPNLIEATQDCGAFVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLCEINLPPMQAGS
SIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPIIAHSLFKSVSHLRNGCLTLAERCVKGITANADRL
RATVENSIGIVTALNPYIGYANATAVAQEAHATGGSVYEIVLRKGLLSKEELDHILRPETLTQPVPLDLHGKSH

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=41.8655097613883, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87082375, Length=461, Percent_Identity=58.351409978308, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1787896, Length=457, Percent_Identity=42.2319474835886, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1790398, Length=311, Percent_Identity=25.08038585209, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=42.0824295010846, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.9785932721713, Blast_Score=263, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=40.997830802603, Blast_Score=357, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24640179, Length=467, Percent_Identity=41.1134903640257, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=462, Percent_Identity=41.3419913419913, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=41.5584415584416, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=40.1727861771058, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=36.7965367965368, Blast_Score=334, Evalue=9e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51081; Mature: 50950

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGPFRTEHDLLGDREVPAQAYYGVHTLRAVENFPITGTPISIYPELIKALAAIKQAAA
CCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
QANHELELLDQARCDAIVAACQELMAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LLGHQKGEYQHLHPNEHVNIGQSTNDVYPTALKVATWFGIRGLVEAMAVLRQAFEAKAEE
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAHIIKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEARLREAAMLICEINLGATAIGTGIT
HHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHEEEEEECCCCCHHCCCCC
AHPSYAPVVLRRLRDITGIPLETAPNLIEATQDCGAFVQLSGVLKRVAVKLSKTCNDLRL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGLCEINLPPMQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQ
HCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEE
LNAFEPIIAHSLFKSVSHLRNGCLTLAERCVKGITANADRLRATVENSIGIVTALNPYIG
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHC
YANATAVAQEAHATGGSVYEIVLRKGLLSKEELDHILRPETLTQPVPLDLHGKSH
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAGPFRTEHDLLGDREVPAQAYYGVHTLRAVENFPITGTPISIYPELIKALAAIKQAAA
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
QANHELELLDQARCDAIVAACQELMAGKLHDQFVVDVIQGGAGTSTNMNANEVIANRALE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LLGHQKGEYQHLHPNEHVNIGQSTNDVYPTALKVATWFGIRGLVEAMAVLRQAFEAKAEE
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAHIIKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEARLREAAMLICEINLGATAIGTGIT
HHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHEEEEEECCCCCHHCCCCC
AHPSYAPVVLRRLRDITGIPLETAPNLIEATQDCGAFVQLSGVLKRVAVKLSKTCNDLRL
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGLCEINLPPMQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAGQLQ
HCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEE
LNAFEPIIAHSLFKSVSHLRNGCLTLAERCVKGITANADRLRATVENSIGIVTALNPYIG
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHC
YANATAVAQEAHATGGSVYEIVLRKGLLSKEELDHILRPETLTQPVPLDLHGKSH
CCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]