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The map label for this gene is dctA [H]

Identifier: 73541408

GI number: 73541408

Start: 1856997

End: 1858430

Strand: Reverse

Name: dctA [H]

Synonym: Reut_A1719

Alternate gene names: 73541408

Gene position: 1858430-1856997 (Counterclockwise)

Preceding gene: 73541409

Following gene: 73541407

Centisome position: 48.82

GC content: 69.39

Gene sequence:

>1434_bases
ATGAAAGCCCTGCGCTGGCTCTCCAACTCCTTTCCGGGCCTGCTGCTGTGCCTGGTGGCCGGCGCTGGCGCGGGCATGGT
GATGGCGCCGCTCGGCACGCTGGCATGGCTGTTCGGACAGCTTTACCTGTCGGTGGTCAACATGGCGTCGGTGCCGCTGC
TGGTGGTGGCCACCTTCTTCGGCCTGCGCCAGGTGCTGACGCTGCCACGGCCGGGCCTGCGCACCGGCACCTTGCTCGGC
GTCGGCGTGGCGCTGCTCGCACTGTGCGCGGTGGCGGGCACGCTGGCCGGCGTGCTGATGGCGCCCGGTGCCGGCCTGAC
ACACAGCGCGGGCGGCGAGCTGGGCCAGCTGATCATGAAGCAGGTGGCCGATGGTGGCGAGGTCCGCATGGACCTGGCCG
GGCGGGGCGCGGACGACAGCGCGGCAGGGAGTTCGGGAGTGCCGGCGGCGGAGCTGGAAGACATCGTCCCCGACAACTTC
TATCGCGCGCTGGCGCAGGGCCACACGCTCGGCATCCTGACCGGCACCATCCTGTTCGGCCTGGCGTTCGCGGCCCTGTC
GCGCGAGCAGACCAGCATGCTCAGCAACGTCTTCGAGGGCATCTACCGCACGTTCGAAAGCATCATTGCCCACGCCAACC
TGTTGCTGCCGGTACTGGCGTTCGGGCTGGCTGCACACCTGACCATGCATAGCGATTCGGCCACGGTACAGGCCATGGCC
GGGGTGCTGTGCAGTTTTACGGTGCTGTGCCTGCTGTTGAGCGTGGCTGCGGCGCTGGCTATCGCGCGCTGCGCGCGGCT
GCCGCTGGTGCAGGTAATGACCGAACTGAAGGGGCCGATGATGGTCGGCCTGGCGTCGGGCAGCGCGACGGCGCCGATCC
CGCATGCGATCGAGGTAATGAGCACGCGCCTGGGCTTTCCGCGAGGGCTGGTGGAACTGACGGTGCCGTTCGGGTCGGTC
TTCCTGCGCGCCGGCTCGGCGCTGTATTTCGGGCTGGTGGCGGTGTTCGTGGCCAACCTGTACGGGCACCCGATCGGTGC
CGGCGAGCTGGTCTGGATTGCCGGCGCCGCCACCGTTGCTGCCTGCCTGTCGGCAGGGCAGAACGGCGTTGGCACGGTGG
CTTACGCCGGTGTGGTGATGTCCGCGCTGCAGTTGCCGGTAGAAGCCGCCGGCGTGCTGCTGCTGGCGGTGGACCCGATC
TGCGACGGTCCGCGCAACGCGCTCAGCCTGATCTGCACTTGCGCAGTCGTGGCGCTGGTGTCGGTGGGCCTGCCCAGCGA
ACGCCCGGCGCCGCAGCCGGTACAGATGGAGCAGCGCGGCGGCGTGGTGCTGCGGCTGGTATTTACACGCGCGCAAATGA
TGCTGCTCGGCGCCTGCGGGCTGGCCGCGGCGGCACTGATCGTGCTGATGGGAATCGGGGTGGGCGCCAAATGA

Upstream 100 bases:

>100_bases
GCCAGCTCCGCTCTGCGCCGCTGACGATGATCGACAGCACCCTCGAACTGGCGCGCGCCACCGTGGCCTTCGCCATGGAG
CGCGGCTGGAACAAGGCCCA

Downstream 100 bases:

>100_bases
TGGCCGTCTGCCGACAGTCTGGAGACATGCAATGAAGGTAGTGTCGATCTGGCGTGGCATTGCGCGTGGGGCCGGCGGGC
TGCTCCTGGCGGGCGCGCTG

Product: Sodium:dicarboxylate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKALRWLSNSFPGLLLCLVAGAGAGMVMAPLGTLAWLFGQLYLSVVNMASVPLLVVATFFGLRQVLTLPRPGLRTGTLLG
VGVALLALCAVAGTLAGVLMAPGAGLTHSAGGELGQLIMKQVADGGEVRMDLAGRGADDSAAGSSGVPAAELEDIVPDNF
YRALAQGHTLGILTGTILFGLAFAALSREQTSMLSNVFEGIYRTFESIIAHANLLLPVLAFGLAAHLTMHSDSATVQAMA
GVLCSFTVLCLLLSVAAALAIARCARLPLVQVMTELKGPMMVGLASGSATAPIPHAIEVMSTRLGFPRGLVELTVPFGSV
FLRAGSALYFGLVAVFVANLYGHPIGAGELVWIAGAATVAACLSAGQNGVGTVAYAGVVMSALQLPVEAAGVLLLAVDPI
CDGPRNALSLICTCAVVALVSVGLPSERPAPQPVQMEQRGGVVLRLVFTRAQMMLLGACGLAAAALIVLMGIGVGAK

Sequences:

>Translated_477_residues
MKALRWLSNSFPGLLLCLVAGAGAGMVMAPLGTLAWLFGQLYLSVVNMASVPLLVVATFFGLRQVLTLPRPGLRTGTLLG
VGVALLALCAVAGTLAGVLMAPGAGLTHSAGGELGQLIMKQVADGGEVRMDLAGRGADDSAAGSSGVPAAELEDIVPDNF
YRALAQGHTLGILTGTILFGLAFAALSREQTSMLSNVFEGIYRTFESIIAHANLLLPVLAFGLAAHLTMHSDSATVQAMA
GVLCSFTVLCLLLSVAAALAIARCARLPLVQVMTELKGPMMVGLASGSATAPIPHAIEVMSTRLGFPRGLVELTVPFGSV
FLRAGSALYFGLVAVFVANLYGHPIGAGELVWIAGAATVAACLSAGQNGVGTVAYAGVVMSALQLPVEAAGVLLLAVDPI
CDGPRNALSLICTCAVVALVSVGLPSERPAPQPVQMEQRGGVVLRLVFTRAQMMLLGACGLAAAALIVLMGIGVGAK
>Mature_477_residues
MKALRWLSNSFPGLLLCLVAGAGAGMVMAPLGTLAWLFGQLYLSVVNMASVPLLVVATFFGLRQVLTLPRPGLRTGTLLG
VGVALLALCAVAGTLAGVLMAPGAGLTHSAGGELGQLIMKQVADGGEVRMDLAGRGADDSAAGSSGVPAAELEDIVPDNF
YRALAQGHTLGILTGTILFGLAFAALSREQTSMLSNVFEGIYRTFESIIAHANLLLPVLAFGLAAHLTMHSDSATVQAMA
GVLCSFTVLCLLLSVAAALAIARCARLPLVQVMTELKGPMMVGLASGSATAPIPHAIEVMSTRLGFPRGLVELTVPFGSV
FLRAGSALYFGLVAVFVANLYGHPIGAGELVWIAGAATVAACLSAGQNGVGTVAYAGVVMSALQLPVEAAGVLLLAVDPI
CDGPRNALSLICTCAVVALVSVGLPSERPAPQPVQMEQRGGVVLRLVFTRAQMMLLGACGLAAAALIVLMGIGVGAK

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1790514, Length=379, Percent_Identity=25.3298153034301, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789947, Length=262, Percent_Identity=23.6641221374046, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48487; Mature: 48487

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALRWLSNSFPGLLLCLVAGAGAGMVMAPLGTLAWLFGQLYLSVVNMASVPLLVVATFF
CCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRQVLTLPRPGLRTGTLLGVGVALLALCAVAGTLAGVLMAPGAGLTHSAGGELGQLIMK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QVADGGEVRMDLAGRGADDSAAGSSGVPAAELEDIVPDNFYRALAQGHTLGILTGTILFG
HHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
LAFAALSREQTSMLSNVFEGIYRTFESIIAHANLLLPVLAFGLAAHLTMHSDSATVQAMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GVLCSFTVLCLLLSVAAALAIARCARLPLVQVMTELKGPMMVGLASGSATAPIPHAIEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
STRLGFPRGLVELTVPFGSVFLRAGSALYFGLVAVFVANLYGHPIGAGELVWIAGAATVA
HHHCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHH
ACLSAGQNGVGTVAYAGVVMSALQLPVEAAGVLLLAVDPICDGPRNALSLICTCAVVALV
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SVGLPSERPAPQPVQMEQRGGVVLRLVFTRAQMMLLGACGLAAAALIVLMGIGVGAK
HCCCCCCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKALRWLSNSFPGLLLCLVAGAGAGMVMAPLGTLAWLFGQLYLSVVNMASVPLLVVATFF
CCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRQVLTLPRPGLRTGTLLGVGVALLALCAVAGTLAGVLMAPGAGLTHSAGGELGQLIMK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QVADGGEVRMDLAGRGADDSAAGSSGVPAAELEDIVPDNFYRALAQGHTLGILTGTILFG
HHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
LAFAALSREQTSMLSNVFEGIYRTFESIIAHANLLLPVLAFGLAAHLTMHSDSATVQAMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
GVLCSFTVLCLLLSVAAALAIARCARLPLVQVMTELKGPMMVGLASGSATAPIPHAIEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
STRLGFPRGLVELTVPFGSVFLRAGSALYFGLVAVFVANLYGHPIGAGELVWIAGAATVA
HHHCCCCCCCEEEECCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHH
ACLSAGQNGVGTVAYAGVVMSALQLPVEAAGVLLLAVDPICDGPRNALSLICTCAVVALV
HHHHCCCCCCHHHHHHHHHHHHHHCCHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
SVGLPSERPAPQPVQMEQRGGVVLRLVFTRAQMMLLGACGLAAAALIVLMGIGVGAK
HCCCCCCCCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]