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The map label for this gene is mdlB [H]

Identifier: 73541351

GI number: 73541351

Start: 1792609

End: 1793856

Strand: Reverse

Name: mdlB [H]

Synonym: Reut_A1661

Alternate gene names: 73541351

Gene position: 1793856-1792609 (Counterclockwise)

Preceding gene: 73541352

Following gene: 73541350

Centisome position: 47.13

GC content: 63.22

Gene sequence:

>1248_bases
ATGACATCTCGAACAGAAGTTGCAGCAAGCCAGCTTGCACCAAGCGCCGGAATGCCAGCTACGGCTGCTAAGGCTGCTTA
TGGCGTCAACCGCCAGATTCATCGACGCTTGCGCCCTATCCTTTCACTCGCCGATTTTGAAACCGCCGCAAAGCGCGTGC
TTCCGCGCCCGATCTTTGGCTACATTGCCGGGGCGGCGGAGGATGAAAAGTCGCTCGCCGCCAACCGGTCGGCATTCGAC
GCGGTCAGGTTTCGCCCCAGGGTCCTGGTCGACGTCTCTGGACGATCGCAAGCGACCGAGATCTTCGGGCAGCGCTACGC
GTCTCCCTTTGGCATTGCGCCCGTCGGAATCAGCGCGATTGCCGCTTACCGTGGCGACGTGGTGCTGGCGCAAGCCGCGC
GCGACGCGCAAATACCCGCCATCATGAGCGGCACGTCGCTGATTCCGATGGAAGAGGTCCACGCTGCCGCGCCCGGCACG
TGGTTCCAGGCGTACCTGCCGGGCGATGCCCTGCGGCGGGACGCGCTGATCGAGCGGATTTGCGCGGCGGGATTCGGTAC
CCTGGTCCTGACCGTGGATATTCCCGTCTGGGCCAATCGGGAGAACAACGTCCGTACTGGCTTCTCGCTGCCCTTGCGGC
CGTCTGTCCGCCTGGCATTTGATGGTGTGTCCCGTCCGCGCTGGCTGGCCGGGACGTTCGCCAGGACGCTTCTCACGAGC
GGCATGCCGCATTTCGAGAATTCCTTTGCCACGCGCGGCGCACCGATCCTGTCGGCCTCGGCGATTCGCGACACAACAGG
ACGCGATCACCTGAACTGGATAGACATCGAACGCATCCGGCAAAGATGGCCCGGCAATCTGGTTATCAAGGGGATTTTGC
ACAAGGCTGATGCAGAGCGCGCTGTCGCGTTGGGCGCCGACGGCATCATCGTCTCGAATCACGGCGGCCGCCAACTGGAC
GGTGCCGTCGAGCCGCTTGCCGTACTGCCGGAAATCTGTGACAGCGTCGGGCACAATACCGCCGTCATGATGGACAGTGG
TATCCGGCGCGGCGGTGATGTGCTGAAAGCCCTTGCATTGGGCGCCCGCTTTGTCTTCCTGGGGCGGCCCTTTATCTACG
CAGCGTCCGTGGGTGGACCCGAGGGCGTTTGCCACGCCATCACGCTCTTGCGCGACGAAGTCGATCGAAACATGGCTATG
CTTGGGGCGAACACCGTCGCCGACGTGAACCGTTCCGTCATTGTCTAG

Upstream 100 bases:

>100_bases
GGAACTGATGCGCCAGCGCGGGACATTCGCCTGAACATGTAATGGGTTCCGGCACGGCTGGCCGTGACAGCTGCCGGGCT
GGACAGAGGCTTCAAACAAG

Downstream 100 bases:

>100_bases
TTCCGTGCACCATTGAAGCCGCACCGGGCGGAATCGCGCCGGTCGCAAGAAGGGAATGCCATGACTCAAGCAAAAGCCAT
TTATCTCGATCCGCCGGCGG

Product: L-lactate dehydrogenase (cytochrome)

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTSRTEVAASQLAPSAGMPATAAKAAYGVNRQIHRRLRPILSLADFETAAKRVLPRPIFGYIAGAAEDEKSLAANRSAFD
AVRFRPRVLVDVSGRSQATEIFGQRYASPFGIAPVGISAIAAYRGDVVLAQAARDAQIPAIMSGTSLIPMEEVHAAAPGT
WFQAYLPGDALRRDALIERICAAGFGTLVLTVDIPVWANRENNVRTGFSLPLRPSVRLAFDGVSRPRWLAGTFARTLLTS
GMPHFENSFATRGAPILSASAIRDTTGRDHLNWIDIERIRQRWPGNLVIKGILHKADAERAVALGADGIIVSNHGGRQLD
GAVEPLAVLPEICDSVGHNTAVMMDSGIRRGGDVLKALALGARFVFLGRPFIYAASVGGPEGVCHAITLLRDEVDRNMAM
LGANTVADVNRSVIV

Sequences:

>Translated_415_residues
MTSRTEVAASQLAPSAGMPATAAKAAYGVNRQIHRRLRPILSLADFETAAKRVLPRPIFGYIAGAAEDEKSLAANRSAFD
AVRFRPRVLVDVSGRSQATEIFGQRYASPFGIAPVGISAIAAYRGDVVLAQAARDAQIPAIMSGTSLIPMEEVHAAAPGT
WFQAYLPGDALRRDALIERICAAGFGTLVLTVDIPVWANRENNVRTGFSLPLRPSVRLAFDGVSRPRWLAGTFARTLLTS
GMPHFENSFATRGAPILSASAIRDTTGRDHLNWIDIERIRQRWPGNLVIKGILHKADAERAVALGADGIIVSNHGGRQLD
GAVEPLAVLPEICDSVGHNTAVMMDSGIRRGGDVLKALALGARFVFLGRPFIYAASVGGPEGVCHAITLLRDEVDRNMAM
LGANTVADVNRSVIV
>Mature_414_residues
TSRTEVAASQLAPSAGMPATAAKAAYGVNRQIHRRLRPILSLADFETAAKRVLPRPIFGYIAGAAEDEKSLAANRSAFDA
VRFRPRVLVDVSGRSQATEIFGQRYASPFGIAPVGISAIAAYRGDVVLAQAARDAQIPAIMSGTSLIPMEEVHAAAPGTW
FQAYLPGDALRRDALIERICAAGFGTLVLTVDIPVWANRENNVRTGFSLPLRPSVRLAFDGVSRPRWLAGTFARTLLTSG
MPHFENSFATRGAPILSASAIRDTTGRDHLNWIDIERIRQRWPGNLVIKGILHKADAERAVALGADGIIVSNHGGRQLDG
AVEPLAVLPEICDSVGHNTAVMMDSGIRRGGDVLKALALGARFVFLGRPFIYAASVGGPEGVCHAITLLRDEVDRNMAML
GANTVADVNRSVIV

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=377, Percent_Identity=33.9522546419098, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI7705393, Length=377, Percent_Identity=33.9522546419098, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI11068137, Length=377, Percent_Identity=33.1564986737401, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1790033, Length=385, Percent_Identity=38.4415584415584, Blast_Score=232, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI193208036, Length=377, Percent_Identity=31.2997347480106, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI32566217, Length=303, Percent_Identity=30.03300330033, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323587, Length=390, Percent_Identity=30.5128205128205, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI281363140, Length=413, Percent_Identity=34.1404358353511, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78707190, Length=413, Percent_Identity=34.1404358353511, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI78707188, Length=378, Percent_Identity=35.1851851851852, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 44355; Mature: 44224

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTEVAASQLAPSAGMPATAAKAAYGVNRQIHRRLRPILSLADFETAAKRVLPRPIFG
CCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YIAGAAEDEKSLAANRSAFDAVRFRPRVLVDVSGRSQATEIFGQRYASPFGIAPVGISAI
HHCCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
AAYRGDVVLAQAARDAQIPAIMSGTSLIPMEEVHAAAPGTWFQAYLPGDALRRDALIERI
HHHCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
CAAGFGTLVLTVDIPVWANRENNVRTGFSLPLRPSVRLAFDGVSRPRWLAGTFARTLLTS
HHCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
GMPHFENSFATRGAPILSASAIRDTTGRDHLNWIDIERIRQRWPGNLVIKGILHKADAER
CCCCHHCCHHCCCCCCEEHHHHHCCCCCCCCCEECHHHHHHHCCCCEEEHHHHHHCCCCC
AVALGADGIIVSNHGGRQLDGAVEPLAVLPEICDSVGHNTAVMMDSGIRRGGDVLKALAL
EEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHH
GARFVFLGRPFIYAASVGGPEGVCHAITLLRDEVDRNMAMLGANTVADVNRSVIV
HCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure 
TSRTEVAASQLAPSAGMPATAAKAAYGVNRQIHRRLRPILSLADFETAAKRVLPRPIFG
CCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
YIAGAAEDEKSLAANRSAFDAVRFRPRVLVDVSGRSQATEIFGQRYASPFGIAPVGISAI
HHCCCCCCHHHHHHCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHH
AAYRGDVVLAQAARDAQIPAIMSGTSLIPMEEVHAAAPGTWFQAYLPGDALRRDALIERI
HHHCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
CAAGFGTLVLTVDIPVWANRENNVRTGFSLPLRPSVRLAFDGVSRPRWLAGTFARTLLTS
HHCCCCEEEEEEECCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHC
GMPHFENSFATRGAPILSASAIRDTTGRDHLNWIDIERIRQRWPGNLVIKGILHKADAER
CCCCHHCCHHCCCCCCEEHHHHHCCCCCCCCCEECHHHHHHHCCCCEEEHHHHHHCCCCC
AVALGADGIIVSNHGGRQLDGAVEPLAVLPEICDSVGHNTAVMMDSGIRRGGDVLKALAL
EEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHH
GARFVFLGRPFIYAASVGGPEGVCHAITLLRDEVDRNMAMLGANTVADVNRSVIV
HCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]