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The map label for this gene is yogA [H]

Identifier: 73541350

GI number: 73541350

Start: 1791535

End: 1792548

Strand: Reverse

Name: yogA [H]

Synonym: Reut_A1660

Alternate gene names: 73541350

Gene position: 1792548-1791535 (Counterclockwise)

Preceding gene: 73541351

Following gene: 73541349

Centisome position: 47.09

GC content: 65.48

Gene sequence:

>1014_bases
ATGACTCAAGCAAAAGCCATTTATCTCGATCCGCCGGCGGGTTTCGATCACATCGTCGTCGGCACCGCTGACGTTGCGCC
GCCGGGGCCGGGCGAGATCACCGTCCGCATTCGGGCCAGCTCGCTCAATTATCACGACTACGCCGTGGTTTCCGGCCGCA
GGAGCCTGACGGAACGACGCATTCCGATGGCGGACGGCGCCGGAGAAGTCACAGCTGTCGGCGCGGACGTCAAGGGTTTC
TCGGTGGGCGACCATGTCGTCAGCACCTTCTTCCCGACGTGGCAGGACGGCGAGCCGCGATTGTGCGATTTCGCTACCGT
CCCCGGAGATGGCGTCGACGGCTTTGCGCGCGACGCCGTGACGATGCCGCAAGATTGCTTCACGCTCGCGCCGCGCGGCT
ACAGCCATGCCGAGGCCGCGACGCTGACCACCGCCGGACTGACAGCCTGGCGGGCACTGTTTGTCGACGGCGCCCTGAAG
CCCGGAGAGACCGTGCTGGTACTTGGGACCGGTGGCGTATCGGTCTTTGCCCTGCAGTTCGCCAAAGCGGCCGGCGCGCG
CGTGATCGCCACTTCCTCGTCGGACGAGAAGCTCGACAGGCTTCGGGAGCTTGGCGCGGATTGCCTGATCAACTATCGCA
AGACGGCGCAGTGGGGCGAGACCGTGCGCGATATGACGGACGGCGCCGGCGCCGACCATGTGATCGAGGTAGGCGGCGCC
GCAACGCTGGATCAATCGATGACGGCAGCCCGTGTCGGCGGCCATATCGCCGTGATCGGCGCGCTGGGGAACGCGACCGC
GCCGATTACTGTCACGCAGCTGCTGCGCAAGCATCTGCGTCTGACCGGCCTGCTCGTCGGGAGTCGCCGACACCAGCTTG
ACATGGTGCGTGCCCTCGATGCCACCGGCATCCGCCCGGTCATTGACCGCCACTTTCCGCTCGAATCCATGGCTGACGCG
TTTCGCTACCAGGCGTCGAACCAGCATTTTGGCAAGATTGTGCTGGACATCTGA

Upstream 100 bases:

>100_bases
GAACACCGTCGCCGACGTGAACCGTTCCGTCATTGTCTAGTTCCGTGCACCATTGAAGCCGCACCGGGCGGAATCGCGCC
GGTCGCAAGAAGGGAATGCC

Downstream 100 bases:

>100_bases
GCGGGCGTCTGCCTCGCTGAAGGACGCATGGTTACCATGCGCAGCCGCTGACTCCCTGGCATCAGTCCAGCCTGCCCGAA
ACATCGCCTAGTCCGATAAG

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTQAKAIYLDPPAGFDHIVVGTADVAPPGPGEITVRIRASSLNYHDYAVVSGRRSLTERRIPMADGAGEVTAVGADVKGF
SVGDHVVSTFFPTWQDGEPRLCDFATVPGDGVDGFARDAVTMPQDCFTLAPRGYSHAEAATLTTAGLTAWRALFVDGALK
PGETVLVLGTGGVSVFALQFAKAAGARVIATSSSDEKLDRLRELGADCLINYRKTAQWGETVRDMTDGAGADHVIEVGGA
ATLDQSMTAARVGGHIAVIGALGNATAPITVTQLLRKHLRLTGLLVGSRRHQLDMVRALDATGIRPVIDRHFPLESMADA
FRYQASNQHFGKIVLDI

Sequences:

>Translated_337_residues
MTQAKAIYLDPPAGFDHIVVGTADVAPPGPGEITVRIRASSLNYHDYAVVSGRRSLTERRIPMADGAGEVTAVGADVKGF
SVGDHVVSTFFPTWQDGEPRLCDFATVPGDGVDGFARDAVTMPQDCFTLAPRGYSHAEAATLTTAGLTAWRALFVDGALK
PGETVLVLGTGGVSVFALQFAKAAGARVIATSSSDEKLDRLRELGADCLINYRKTAQWGETVRDMTDGAGADHVIEVGGA
ATLDQSMTAARVGGHIAVIGALGNATAPITVTQLLRKHLRLTGLLVGSRRHQLDMVRALDATGIRPVIDRHFPLESMADA
FRYQASNQHFGKIVLDI
>Mature_336_residues
TQAKAIYLDPPAGFDHIVVGTADVAPPGPGEITVRIRASSLNYHDYAVVSGRRSLTERRIPMADGAGEVTAVGADVKGFS
VGDHVVSTFFPTWQDGEPRLCDFATVPGDGVDGFARDAVTMPQDCFTLAPRGYSHAEAATLTTAGLTAWRALFVDGALKP
GETVLVLGTGGVSVFALQFAKAAGARVIATSSSDEKLDRLRELGADCLINYRKTAQWGETVRDMTDGAGADHVIEVGGAA
TLDQSMTAARVGGHIAVIGALGNATAPITVTQLLRKHLRLTGLLVGSRRHQLDMVRALDATGIRPVIDRHFPLESMADAF
RYQASNQHFGKIVLDI

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=349, Percent_Identity=25.5014326647564, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI22538444, Length=349, Percent_Identity=25.5014326647564, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI47519420, Length=357, Percent_Identity=28.0112044817927, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194239674, Length=310, Percent_Identity=26.1290322580645, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI13236495, Length=310, Percent_Identity=26.1290322580645, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI18379349, Length=334, Percent_Identity=25.748502994012, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI28557745, Length=339, Percent_Identity=30.0884955752212, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI156627571, Length=323, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI41872631, Length=326, Percent_Identity=26.3803680981595, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081918, Length=316, Percent_Identity=25.9493670886076, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789651, Length=287, Percent_Identity=27.1777003484321, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87082125, Length=340, Percent_Identity=24.4117647058824, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790045, Length=332, Percent_Identity=25.3012048192771, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788073, Length=212, Percent_Identity=30.188679245283, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=316, Percent_Identity=28.4810126582279, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=326, Percent_Identity=25.7668711656442, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562878, Length=215, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=323, Percent_Identity=25.6965944272446, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=333, Percent_Identity=27.3273273273273, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=355, Percent_Identity=25.6338028169014, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=332, Percent_Identity=26.2048192771084, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=290, Percent_Identity=26.8965517241379, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=347, Percent_Identity=24.7838616714697, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24581345, Length=223, Percent_Identity=30.0448430493274, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19920632, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221330659, Length=222, Percent_Identity=29.2792792792793, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 35677; Mature: 35546

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAKAIYLDPPAGFDHIVVGTADVAPPGPGEITVRIRASSLNYHDYAVVSGRRSLTERR
CCCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHCC
IPMADGAGEVTAVGADVKGFSVGDHVVSTFFPTWQDGEPRLCDFATVPGDGVDGFARDAV
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHCCC
TMPQDCFTLAPRGYSHAEAATLTTAGLTAWRALFVDGALKPGETVLVLGTGGVSVFALQF
CCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
AKAAGARVIATSSSDEKLDRLRELGADCLINYRKTAQWGETVRDMTDGAGADHVIEVGGA
HHHCCCEEEEECCCHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
ATLDQSMTAARVGGHIAVIGALGNATAPITVTQLLRKHLRLTGLLVGSRRHQLDMVRALD
CHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHC
ATGIRPVIDRHFPLESMADAFRYQASNQHFGKIVLDI
CCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEC
>Mature Secondary Structure 
TQAKAIYLDPPAGFDHIVVGTADVAPPGPGEITVRIRASSLNYHDYAVVSGRRSLTERR
CCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCEEEECCCHHHHHCC
IPMADGAGEVTAVGADVKGFSVGDHVVSTFFPTWQDGEPRLCDFATVPGDGVDGFARDAV
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHCCC
TMPQDCFTLAPRGYSHAEAATLTTAGLTAWRALFVDGALKPGETVLVLGTGGVSVFALQF
CCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
AKAAGARVIATSSSDEKLDRLRELGADCLINYRKTAQWGETVRDMTDGAGADHVIEVGGA
HHHCCCEEEEECCCHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
ATLDQSMTAARVGGHIAVIGALGNATAPITVTQLLRKHLRLTGLLVGSRRHQLDMVRALD
CHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHC
ATGIRPVIDRHFPLESMADAFRYQASNQHFGKIVLDI
CCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]