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The map label for this gene is pcpR [H]

Identifier: 73541273

GI number: 73541273

Start: 1706153

End: 1707124

Strand: Reverse

Name: pcpR [H]

Synonym: Reut_A1583

Alternate gene names: 73541273

Gene position: 1707124-1706153 (Counterclockwise)

Preceding gene: 73541281

Following gene: 73541272

Centisome position: 44.85

GC content: 66.05

Gene sequence:

>972_bases
ATGGACACTATTCCCCAGCGTCATGAAGACGAACTCAGCCTGAATCAACTGCTGGTGCTGGATGTACTGCTGAGCGAGCG
CAGCCTCACCAAGGCGGCGCGCGTGCTCAACCTGACGCAGCCTGCTTTGAGCAAGACGTTGGCGCGTCTGCGCCAGTATT
TCGGTGACCCGCTGTTCGTCCGCGTCGGCCTGCGCATGGAGCCGACCCCCAAGGCACTGGAGCTTGCTGATCCGGTGCGG
GGCATTCTCCAGAGCGTGCGCGCGCTGCGTTCGGACCATGTCGGCTTCGATCCGCGCACCAGCCAGCGCAGCTTCAGCTT
CTTCCTGCTCGATGCCGGCGCCTCGCAGATGCTGCCACCGCTGCTGAGCCGGCTGGCGGAAGAAGCACCGGGCATCCTGG
TGCAGGCGGTCAACGCCGATGCCACGCATCTCGACATCTGGCTGGAGAACGGCCTTGTCGACCTGGCCATCGGTTCGTTC
CCGGGCCTCACGCAGAGCATCCGCCGGCAGTTGCTATGGCACGAAACCTACGGCGCGGTCGTGCGCAAAGACCATCCGCG
GCTCGCTCAACTGGGAGACCTGGATGCCTTCGTCGCCGAGAAGCATGTGCTGGTATCCGCGGTCGGTACGGGCCACCAGC
ACCTGAGCATCGAGCGCGCCATCGAGGCCGCAGTGCCGCACACCAACATCATCTGCCGCGTGCCCAGCTTCGCCACCGCG
GGCCTGATCGCCAAGCATTCGGACGCCGTGGCGGTCCTGCCGCTAACGCTGGCACGCGCGCTTTGCAAGGATCTCGACCT
GACGGTTGTCATGCCACCGCTGACGCTGCCCGGCATGGACATCGCCCAACACTGGCACGACCGCGTGCACCGCGAGCCCG
GCAACCAGTGGATCCGTGCGCTCGTACACAGGCTGTTCGCCCGGCACGATGCCGTGGCGGGCGCGGCTGCCGGGGCGAAC
ATGGAAGGATGA

Upstream 100 bases:

>100_bases
TGAATGAAGCTTTGTCTCTGGAAACCTGCTTTCTGATCTGTGTCAGCTGTGTGAAACTAGACTACAGCCGAGCCGGCCCG
TTTGCTAATTACCTCGCATC

Downstream 100 bases:

>100_bases
GCGCCGGTTATAGACACCATGACGGCAAAGGATTGGCGTCCTGCGCCATCGCCTCCTAGCATGCGGTGCATGACAAGAGG
AGGAGAGCATCATGCAGGTA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDTIPQRHEDELSLNQLLVLDVLLSERSLTKAARVLNLTQPALSKTLARLRQYFGDPLFVRVGLRMEPTPKALELADPVR
GILQSVRALRSDHVGFDPRTSQRSFSFFLLDAGASQMLPPLLSRLAEEAPGILVQAVNADATHLDIWLENGLVDLAIGSF
PGLTQSIRRQLLWHETYGAVVRKDHPRLAQLGDLDAFVAEKHVLVSAVGTGHQHLSIERAIEAAVPHTNIICRVPSFATA
GLIAKHSDAVAVLPLTLARALCKDLDLTVVMPPLTLPGMDIAQHWHDRVHREPGNQWIRALVHRLFARHDAVAGAAAGAN
MEG

Sequences:

>Translated_323_residues
MDTIPQRHEDELSLNQLLVLDVLLSERSLTKAARVLNLTQPALSKTLARLRQYFGDPLFVRVGLRMEPTPKALELADPVR
GILQSVRALRSDHVGFDPRTSQRSFSFFLLDAGASQMLPPLLSRLAEEAPGILVQAVNADATHLDIWLENGLVDLAIGSF
PGLTQSIRRQLLWHETYGAVVRKDHPRLAQLGDLDAFVAEKHVLVSAVGTGHQHLSIERAIEAAVPHTNIICRVPSFATA
GLIAKHSDAVAVLPLTLARALCKDLDLTVVMPPLTLPGMDIAQHWHDRVHREPGNQWIRALVHRLFARHDAVAGAAAGAN
MEG
>Mature_323_residues
MDTIPQRHEDELSLNQLLVLDVLLSERSLTKAARVLNLTQPALSKTLARLRQYFGDPLFVRVGLRMEPTPKALELADPVR
GILQSVRALRSDHVGFDPRTSQRSFSFFLLDAGASQMLPPLLSRLAEEAPGILVQAVNADATHLDIWLENGLVDLAIGSF
PGLTQSIRRQLLWHETYGAVVRKDHPRLAQLGDLDAFVAEKHVLVSAVGTGHQHLSIERAIEAAVPHTNIICRVPSFATA
GLIAKHSDAVAVLPLTLARALCKDLDLTVVMPPLTLPGMDIAQHWHDRVHREPGNQWIRALVHRLFARHDAVAGAAAGAN
MEG

Specific function: Transcriptional activator for the pcpA, pcpB and pcpE genes for pentachlorophenol (PCP) degradation. Essential for PCP degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=300, Percent_Identity=28, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790147, Length=305, Percent_Identity=26.5573770491803, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081766, Length=285, Percent_Identity=22.1052631578947, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786820, Length=288, Percent_Identity=23.6111111111111, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35407; Mature: 35407

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTIPQRHEDELSLNQLLVLDVLLSERSLTKAARVLNLTQPALSKTLARLRQYFGDPLFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEE
RVGLRMEPTPKALELADPVRGILQSVRALRSDHVGFDPRTSQRSFSFFLLDAGASQMLPP
EECCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LLSRLAEEAPGILVQAVNADATHLDIWLENGLVDLAIGSFPGLTQSIRRQLLWHETYGAV
HHHHHHHHCCCEEEEEECCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VRKDHPRLAQLGDLDAFVAEKHVLVSAVGTGHQHLSIERAIEAAVPHTNIICRVPSFATA
HCCCCCCHHHHCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHH
GLIAKHSDAVAVLPLTLARALCKDLDLTVVMPPLTLPGMDIAQHWHDRVHREPGNQWIRA
HHHEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHH
LVHRLFARHDAVAGAAAGANMEG
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTIPQRHEDELSLNQLLVLDVLLSERSLTKAARVLNLTQPALSKTLARLRQYFGDPLFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCEEE
RVGLRMEPTPKALELADPVRGILQSVRALRSDHVGFDPRTSQRSFSFFLLDAGASQMLPP
EECCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LLSRLAEEAPGILVQAVNADATHLDIWLENGLVDLAIGSFPGLTQSIRRQLLWHETYGAV
HHHHHHHHCCCEEEEEECCCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
VRKDHPRLAQLGDLDAFVAEKHVLVSAVGTGHQHLSIERAIEAAVPHTNIICRVPSFATA
HCCCCCCHHHHCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCHHH
GLIAKHSDAVAVLPLTLARALCKDLDLTVVMPPLTLPGMDIAQHWHDRVHREPGNQWIRA
HHHEECCCCEEHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHH
LVHRLFARHDAVAGAAAGANMEG
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12169590 [H]