Click here to switch to the map view.

The map label for this gene is echA8 [H]

Identifier: 73541252

GI number: 73541252

Start: 1682656

End: 1683432

Strand: Direct

Name: echA8 [H]

Synonym: Reut_A1562

Alternate gene names: 73541252

Gene position: 1682656-1683432 (Clockwise)

Preceding gene: 73541251

Following gene: 73541253

Centisome position: 44.2

GC content: 67.05

Gene sequence:

>777_bases
ATGCAAACGAATCAAACCGCCCAGAAGGCGCCGTTTCGCGTCTACATCGGCGATGGCATCGCCGAACTGGTGATTGATCA
TCCCCCCGTCAATGCGCTCGACAATGCCGGTTGGCAGGCGCTGGCACGGGCAATCGACCGCCTCGGCGATGACCCGACGG
TGCGCGTGATCCTGCTGCGCGGCGAAGGCCGTGGCTTCTGCGCGGGCGTCGATATCAAAGAACTGGCGAAGCATCCGCAG
CGCATCGTCGACGTCAACGCCGGTAACTACGCGACGTTCCGCGCCGTGCACCGCAATCCCAAGCCGGTCATTGTCGCCGT
GCACGGCTTCGTGCTGGGTGGCGGCATCGGCATCTGCGGTGCGGCCGACATCATCGTGGCGTCCGAGTGCGCAAGCTTTG
GCGTGCCGGAGATCGACCGCGGCGCCATGGGCGGCGGCGCCCACCTGCAGCGCATGTTCGGTGTGCAGAAGGTGCGCGCG
ATGTACTTCACCGGCGAGATGATCGATGCGGCCGAGGCCTTCCGCCTCGGTGCGGTCGAGCGTGTCGTGCCGCGCGACGC
ATTGCGCGACGAGGCCCTGGCGCTGGCCCGCAAGATTGCCGCCAAGAGCCCGGCTATGCTGCAGCTTGCCAAGGAAGCGC
TCAACGGCGTCGAGGACGGCAACCTCGAGGACAAGTACCGCTGGGAGCAGGGCTTCACGCTGCAGGCCTATATGAGCGCC
GATTCCGGCGAGGCGCGCGCCGCCTTCGTGGAAGGCCGCGAGGCCCGCTTCGCCTAG

Upstream 100 bases:

>100_bases
GCAGATTGTCGCGGGCATCGCTGCCCAGGCGCAGGTGCGGCTGGCCGCGTTGTCCGGCACCGGGCTGGCTGCTGCCGCCT
GACCACAGACAGAGGAAACC

Downstream 100 bases:

>100_bases
CCCGGGAATCCACCATGCATCTCGAATACACCGACGCCCAGCGCGCTTTCCGCGCCGAGGTCCGCGCGTGGATGGCCGAA
CACGTGCCGCGCACGCCGCT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MQTNQTAQKAPFRVYIGDGIAELVIDHPPVNALDNAGWQALARAIDRLGDDPTVRVILLRGEGRGFCAGVDIKELAKHPQ
RIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVASECASFGVPEIDRGAMGGGAHLQRMFGVQKVRA
MYFTGEMIDAAEAFRLGAVERVVPRDALRDEALALARKIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSA
DSGEARAAFVEGREARFA

Sequences:

>Translated_258_residues
MQTNQTAQKAPFRVYIGDGIAELVIDHPPVNALDNAGWQALARAIDRLGDDPTVRVILLRGEGRGFCAGVDIKELAKHPQ
RIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVASECASFGVPEIDRGAMGGGAHLQRMFGVQKVRA
MYFTGEMIDAAEAFRLGAVERVVPRDALRDEALALARKIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSA
DSGEARAAFVEGREARFA
>Mature_258_residues
MQTNQTAQKAPFRVYIGDGIAELVIDHPPVNALDNAGWQALARAIDRLGDDPTVRVILLRGEGRGFCAGVDIKELAKHPQ
RIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVASECASFGVPEIDRGAMGGGAHLQRMFGVQKVRA
MYFTGEMIDAAEAFRLGAVERVVPRDALRDEALALARKIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSA
DSGEARAAFVEGREARFA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=232, Percent_Identity=33.1896551724138, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI70995211, Length=243, Percent_Identity=30.4526748971193, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI31542718, Length=244, Percent_Identity=30.7377049180328, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI4502327, Length=236, Percent_Identity=31.3559322033898, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68989263, Length=188, Percent_Identity=27.1276595744681, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI37594471, Length=241, Percent_Identity=27.8008298755187, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI37594469, Length=241, Percent_Identity=27.8008298755187, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=35.1239669421488, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI221142681, Length=243, Percent_Identity=32.9218106995885, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=31.0756972111554, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082183, Length=236, Percent_Identity=28.8135593220339, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=29.2490118577075, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790281, Length=193, Percent_Identity=33.160621761658, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=31.7708333333333, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=251, Percent_Identity=30.6772908366534, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17534483, Length=250, Percent_Identity=27.6, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=251, Percent_Identity=31.4741035856574, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=35.2657004830918, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=212, Percent_Identity=27.8301886792453, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=256, Percent_Identity=28.125, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17558304, Length=174, Percent_Identity=28.735632183908, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=169, Percent_Identity=27.2189349112426, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=249, Percent_Identity=36.5461847389558, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24653477, Length=249, Percent_Identity=36.5461847389558, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=33.6448598130841, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=29.6, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21357171, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=29.8165137614679, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27714; Mature: 27714

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNQTAQKAPFRVYIGDGIAELVIDHPPVNALDNAGWQALARAIDRLGDDPTVRVILLR
CCCCCCHHHCCEEEEECCCHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEE
GEGRGFCAGVDIKELAKHPQRIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICG
CCCCEEEECCCHHHHHCCCHHEEECCCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCC
AADIIVASECASFGVPEIDRGAMGGGAHLQRMFGVQKVRAMYFTGEMIDAAEAFRLGAVE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RVVPRDALRDEALALARKIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSA
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECC
DSGEARAAFVEGREARFA
CCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MQTNQTAQKAPFRVYIGDGIAELVIDHPPVNALDNAGWQALARAIDRLGDDPTVRVILLR
CCCCCCHHHCCEEEEECCCHHHEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEE
GEGRGFCAGVDIKELAKHPQRIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICG
CCCCEEEECCCHHHHHCCCHHEEECCCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCC
AADIIVASECASFGVPEIDRGAMGGGAHLQRMFGVQKVRAMYFTGEMIDAAEAFRLGAVE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RVVPRDALRDEALALARKIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSA
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECC
DSGEARAAFVEGREARFA
CCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]