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The map label for this gene is ilvB [H]

Identifier: 73541188

GI number: 73541188

Start: 1616482

End: 1618230

Strand: Reverse

Name: ilvB [H]

Synonym: Reut_A1497

Alternate gene names: 73541188

Gene position: 1618230-1616482 (Counterclockwise)

Preceding gene: 73541193

Following gene: 73541186

Centisome position: 42.51

GC content: 67.87

Gene sequence:

>1749_bases
ATGCACGATAGGCCGCACAACCCCGCGCGTCAAACGGAACAATTGCAGACATTCATCCGACCAGACCTCGCCATGACCTC
CACTCCTGCCCAGGCCGCCCGCACCGGCGGCCGCGTTCTTGTCGACCAACTGCGCATCCATGGCACCGAACGCATCTTCT
GTGTGCCGGGCGAGAGCTTTCTCGACGTGCTCGACGCGCTGCACGACCAGCCAGCCATCGATCTCGTCGTCTGCAAGCAC
GAGGGCGCAGCCGCAAACATGGCCGAGGCCGACGGCAAGCTGACTGGCCGCCCCGGCATCTGCTTCGTGACGCGCGGACC
GGGCGCCACGCATGGCAGCATCGGCGTGCATATCGCCCGGCAGGACTCGACGCCGATGATCCTGTTCGCGGGCCAGATCG
CGCGGGAGCACAAGGGCCGGGAAGCGTTCCAGGAAGTGGACTACGCCGCAGTGTTTGGCTCGATGGCCAAGTGGGCGGCC
GAGATCGACGATCCGGCCCGGATTCCGGAGATGGTGGCACGTGCGTTCCAGGTCGCCACATCGGGCCGGCCCGGCCCCGT
GGTGCTGGCGCTGCCGGAAGACGTGCTCGACGGCCTCGTGAATTGCGCCGATGCCGCGCCGTACCGGCCGGTTGCCGCCG
CACCGCGTGCCGAGGATGCCAACGCGCTGGCCACTGCCCTGCGCGCCGCGCAGCGTCCGCTGGTCATTGCCGGTGGCGCG
AACTGGACCGAGCAAGCGACCATCGATTTCGCCGCGTTCGTCAGGGCGTGGGATTTGCCGGTCGCCTGTGCCTTCCGCCG
CCAGGACGTGATCGACAACCGCGATCGGCACTACGTCGGCCACCTGAGCCTGGGCGTCAACCCGAAGCTGGCCGAACGCG
TGAAGACGGCCGATCTGATCGTGGCCGTCGGCACGCGGCTCGGCGATATCGCGACAGACGGCTACACGATGCTGCAGGTA
CCGGTGCCGACGCAGAAACTTGTGCATATCCATGCGGACAGCGCGGAACTGGGGCGCGTGTACCAGGCCGCGCTGCCGAT
CCACGCGGGCGTGGCGCCCGCCGCCGCAATGCTGGCCGCCCTGCCCGCGCCGGCTACCGCGCCGTGGAAGGACTGGACCG
CCGGCGCGCGTGCCGACCACGAGGGGTTCGTCGCGCCACCGAAGCCCCATGCCGAACTGACCGGCGTCGATATGATCGCG
GCAATGTCGCACCTGAGCCAGGTCTTGCCCGAGGATGCTGTCGTGACCAACGGCGCCGGCAACTACGCGGTCTGGCTGCA
CCGCTTCTACACCTACAAGCAGTCACGCACGCAACTGGCGCCGACCTGCGGCGCCATGGGCTATGGCTTGCCGGCAGCCA
TCGCGGCCAAGCTGCGCCATCCGCAAAAAACGGTGCTCTGCCTGGCCGGCGACGGCTGCTTCCTGATGTATCCGCAGGAA
ATCGCCACGGCCGCCGCGCATGGCGTGAACCTGGTCGTGATCGTGGTGAACAACGGCATGTACGGCACGATCCGGATGCA
CCAGGAACGCCGCTACCCGGGCCGCCAGAGCGGCACGACCATCGTCAACCCGGATTTTGTGGCCATGGCCAGGTCATGCG
GTGCCTGGGCCGAGCGCGTGGAACGGACGGAAGACTTTGCGGCGGCATTCGAGCGTGCCCGTCAGGCGGGCAAGCCAGCC
GTGCTGGAACTGGTGACCGACCCACTGCAGATTACGCCTGCGATGCGGGTCAGCGATCTGCCGCAATAA

Upstream 100 bases:

>100_bases
CAAGGCAGACCGCGCGTTTCCGTGTCGTTTCCATGCAAGACCCAGCAGCAAATCTACACGGACAGCCACCTCGCCGACTT
GCAGAAATCGCATAACAGAA

Downstream 100 bases:

>100_bases
GGCATATCGGGCCGGGCAACCTGACCCCGCGGGTCACGGTCCCGGCCGCAGCGCGGTCAGCCAGGCTGTTCGTCGTCGGT
CGACTCCTGGAGCAAATGCC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MHDRPHNPARQTEQLQTFIRPDLAMTSTPAQAARTGGRVLVDQLRIHGTERIFCVPGESFLDVLDALHDQPAIDLVVCKH
EGAAANMAEADGKLTGRPGICFVTRGPGATHGSIGVHIARQDSTPMILFAGQIAREHKGREAFQEVDYAAVFGSMAKWAA
EIDDPARIPEMVARAFQVATSGRPGPVVLALPEDVLDGLVNCADAAPYRPVAAAPRAEDANALATALRAAQRPLVIAGGA
NWTEQATIDFAAFVRAWDLPVACAFRRQDVIDNRDRHYVGHLSLGVNPKLAERVKTADLIVAVGTRLGDIATDGYTMLQV
PVPTQKLVHIHADSAELGRVYQAALPIHAGVAPAAAMLAALPAPATAPWKDWTAGARADHEGFVAPPKPHAELTGVDMIA
AMSHLSQVLPEDAVVTNGAGNYAVWLHRFYTYKQSRTQLAPTCGAMGYGLPAAIAAKLRHPQKTVLCLAGDGCFLMYPQE
IATAAAHGVNLVVIVVNNGMYGTIRMHQERRYPGRQSGTTIVNPDFVAMARSCGAWAERVERTEDFAAAFERARQAGKPA
VLELVTDPLQITPAMRVSDLPQ

Sequences:

>Translated_582_residues
MHDRPHNPARQTEQLQTFIRPDLAMTSTPAQAARTGGRVLVDQLRIHGTERIFCVPGESFLDVLDALHDQPAIDLVVCKH
EGAAANMAEADGKLTGRPGICFVTRGPGATHGSIGVHIARQDSTPMILFAGQIAREHKGREAFQEVDYAAVFGSMAKWAA
EIDDPARIPEMVARAFQVATSGRPGPVVLALPEDVLDGLVNCADAAPYRPVAAAPRAEDANALATALRAAQRPLVIAGGA
NWTEQATIDFAAFVRAWDLPVACAFRRQDVIDNRDRHYVGHLSLGVNPKLAERVKTADLIVAVGTRLGDIATDGYTMLQV
PVPTQKLVHIHADSAELGRVYQAALPIHAGVAPAAAMLAALPAPATAPWKDWTAGARADHEGFVAPPKPHAELTGVDMIA
AMSHLSQVLPEDAVVTNGAGNYAVWLHRFYTYKQSRTQLAPTCGAMGYGLPAAIAAKLRHPQKTVLCLAGDGCFLMYPQE
IATAAAHGVNLVVIVVNNGMYGTIRMHQERRYPGRQSGTTIVNPDFVAMARSCGAWAERVERTEDFAAAFERARQAGKPA
VLELVTDPLQITPAMRVSDLPQ
>Mature_582_residues
MHDRPHNPARQTEQLQTFIRPDLAMTSTPAQAARTGGRVLVDQLRIHGTERIFCVPGESFLDVLDALHDQPAIDLVVCKH
EGAAANMAEADGKLTGRPGICFVTRGPGATHGSIGVHIARQDSTPMILFAGQIAREHKGREAFQEVDYAAVFGSMAKWAA
EIDDPARIPEMVARAFQVATSGRPGPVVLALPEDVLDGLVNCADAAPYRPVAAAPRAEDANALATALRAAQRPLVIAGGA
NWTEQATIDFAAFVRAWDLPVACAFRRQDVIDNRDRHYVGHLSLGVNPKLAERVKTADLIVAVGTRLGDIATDGYTMLQV
PVPTQKLVHIHADSAELGRVYQAALPIHAGVAPAAAMLAALPAPATAPWKDWTAGARADHEGFVAPPKPHAELTGVDMIA
AMSHLSQVLPEDAVVTNGAGNYAVWLHRFYTYKQSRTQLAPTCGAMGYGLPAAIAAKLRHPQKTVLCLAGDGCFLMYPQE
IATAAAHGVNLVVIVVNNGMYGTIRMHQERRYPGRQSGTTIVNPDFVAMARSCGAWAERVERTEDFAAAFERARQAGKPA
VLELVTDPLQITPAMRVSDLPQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=24.3339253996448, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI21361361, Length=165, Percent_Identity=32.7272727272727, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI87081685, Length=543, Percent_Identity=29.6500920810313, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=28, Blast_Score=195, Evalue=9e-51,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=25.3141831238779, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=24.2201834862385, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=24.3006993006993, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17542570, Length=577, Percent_Identity=23.9168110918544, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=548, Percent_Identity=24.8175182481752, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=548, Percent_Identity=24.8175182481752, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=26.4054514480409, Blast_Score=221, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6320816, Length=560, Percent_Identity=21.6071428571429, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=23.0612244897959, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=502, Percent_Identity=23.3067729083665, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19922626, Length=560, Percent_Identity=26.25, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62358; Mature: 62358

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDRPHNPARQTEQLQTFIRPDLAMTSTPAQAARTGGRVLVDQLRIHGTERIFCVPGESF
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEHHHCCCCCEEEEECCHHH
LDVLDALHDQPAIDLVVCKHEGAAANMAEADGKLTGRPGICFVTRGPGATHGSIGVHIAR
HHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
QDSTPMILFAGQIAREHKGREAFQEVDYAAVFGSMAKWAAEIDDPARIPEMVARAFQVAT
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGRPGPVVLALPEDVLDGLVNCADAAPYRPVAAAPRAEDANALATALRAAQRPLVIAGGA
CCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCC
NWTEQATIDFAAFVRAWDLPVACAFRRQDVIDNRDRHYVGHLSLGVNPKLAERVKTADLI
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHEE
VAVGTRLGDIATDGYTMLQVPVPTQKLVHIHADSAELGRVYQAALPIHAGVAPAAAMLAA
EECCCHHCCEECCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCHHCCHHHHHHHHHH
LPAPATAPWKDWTAGARADHEGFVAPPKPHAELTGVDMIAAMSHLSQVLPEDAVVTNGAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCC
NYAVWLHRFYTYKQSRTQLAPTCGAMGYGLPAAIAAKLRHPQKTVLCLAGDGCFLMYPQE
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEECHHH
IATAAAHGVNLVVIVVNNGMYGTIRMHQERRYPGRQSGTTIVNPDFVAMARSCGAWAERV
HHHHHHCCCEEEEEEECCCEEEEEEEEHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ERTEDFAAAFERARQAGKPAVLELVTDPLQITPAMRVSDLPQ
HHHHHHHHHHHHHHHCCCCEEEHHHCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MHDRPHNPARQTEQLQTFIRPDLAMTSTPAQAARTGGRVLVDQLRIHGTERIFCVPGESF
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEHHHCCCCCEEEEECCHHH
LDVLDALHDQPAIDLVVCKHEGAAANMAEADGKLTGRPGICFVTRGPGATHGSIGVHIAR
HHHHHHHCCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
QDSTPMILFAGQIAREHKGREAFQEVDYAAVFGSMAKWAAEIDDPARIPEMVARAFQVAT
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
SGRPGPVVLALPEDVLDGLVNCADAAPYRPVAAAPRAEDANALATALRAAQRPLVIAGGA
CCCCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCC
NWTEQATIDFAAFVRAWDLPVACAFRRQDVIDNRDRHYVGHLSLGVNPKLAERVKTADLI
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHEE
VAVGTRLGDIATDGYTMLQVPVPTQKLVHIHADSAELGRVYQAALPIHAGVAPAAAMLAA
EECCCHHCCEECCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCHHCCHHHHHHHHHH
LPAPATAPWKDWTAGARADHEGFVAPPKPHAELTGVDMIAAMSHLSQVLPEDAVVTNGAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCC
NYAVWLHRFYTYKQSRTQLAPTCGAMGYGLPAAIAAKLRHPQKTVLCLAGDGCFLMYPQE
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEECHHH
IATAAAHGVNLVVIVVNNGMYGTIRMHQERRYPGRQSGTTIVNPDFVAMARSCGAWAERV
HHHHHHCCCEEEEEEECCCEEEEEEEEHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ERTEDFAAAFERARQAGKPAVLELVTDPLQITPAMRVSDLPQ
HHHHHHHHHHHHHHHCCCCEEEHHHCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]