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The map label for this gene is mvaB [H]

Identifier: 73541156

GI number: 73541156

Start: 1580632

End: 1581585

Strand: Reverse

Name: mvaB [H]

Synonym: Reut_A1462

Alternate gene names: 73541156

Gene position: 1581585-1580632 (Counterclockwise)

Preceding gene: 73541157

Following gene: 73541151

Centisome position: 41.55

GC content: 65.51

Gene sequence:

>954_bases
ATGGAACGCAACGCCCCTTCCCTGCTCCAGGCGCTGCCGTTGCCTGACGCCGTCAAGATCGTCGAGGTCGGGCCGCGCGA
CGGCCTGCAGAATGAAACGCACCATGTACCGGCCGCGGTCAAGGTCGAACTCGTCAATCGCCTGAGCGAGGCCGGCTTCG
CCAATATCGAGGCCGCATCGTTTGTCTCGCCCAAGTGGGTACCGCAGATGGCCGACGGCGCTGAAGTCATGGGCGCCATC
GCGCGGCGGGCCGGCACCGTCTATTCGGCGCTGACGCCCAATATGAAGGGCTTCGAAGCCGCGCTGGCAGCAGGGGCCGA
CGAGGCGGTCATCTTCGGTGCGGCGAGCGAAGCGTTCTCCCAACGCAATATCAACTGTTCGGTGGCCGAATCGATCGCGC
GTTTCGAGCCCGTGGCGGCTGCGGCCAAGGCGGCCGGCCTGCGCCTGCGCGCCAGCATCTCGTGCGCACTCGGCTGCCCG
TACGAGGGCGAGATCGCGATCGAAAAGGTCGCCGAGGTGGTATCGCGCATGCGCGATCTCGGCTGCGACGAGATCGACAT
CGCCGATACCATCGGCGTCGGCACGCCGCTGCGCACGCAGGCCGTCATGAACGCCGCGGCGCAAGTCTTTCCGCGCGAGC
GGGTGGCGGGACATTTCCACGACACGTATGGCCAGGCACTGCCCAATATCCTGGCCAGCCTGCAGGTCGGTATCCGCATC
TTCCATGCGTCGGTCGCAGGCCTTGGCGGCTGCCCATACGCCAAGGGCGCCTCGGGCAATGTGGCGACGGAAGACCTGCT
TTACATGCTGCATGGCATGGGCATCCATACTGGCATCGATCTTGGCCAGGTGATCCAGGCAGGTGCCTTCATCTCCGAGG
CAATCGGGCGGCCGTATGGGTCGCGTGTTGGCAAGGCATTGCTCGAATGCAGTTGCCAGCAGCAAAGCGCCTGA

Upstream 100 bases:

>100_bases
GCCCCCGCAGGCGGCAAGGTGCAGGAAGTGCTGTACGCCGTGGGGGACCAGGTCCAGGAAGGCGCGCAGCTCGTGACGCT
GGAACAGGAGGCCGCCTGAC

Downstream 100 bases:

>100_bases
GCCTGATGCTTGCGCCTGCCCACATCGCCCGGGTCGCCGCAGCGTCCCGGGCGTTTTACATGGTTACGGCTTCTTCGGCC
GCACGGCATCCGCGGTGCCA

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MERNAPSLLQALPLPDAVKIVEVGPRDGLQNETHHVPAAVKVELVNRLSEAGFANIEAASFVSPKWVPQMADGAEVMGAI
ARRAGTVYSALTPNMKGFEAALAAGADEAVIFGAASEAFSQRNINCSVAESIARFEPVAAAAKAAGLRLRASISCALGCP
YEGEIAIEKVAEVVSRMRDLGCDEIDIADTIGVGTPLRTQAVMNAAAQVFPRERVAGHFHDTYGQALPNILASLQVGIRI
FHASVAGLGGCPYAKGASGNVATEDLLYMLHGMGIHTGIDLGQVIQAGAFISEAIGRPYGSRVGKALLECSCQQQSA

Sequences:

>Translated_317_residues
MERNAPSLLQALPLPDAVKIVEVGPRDGLQNETHHVPAAVKVELVNRLSEAGFANIEAASFVSPKWVPQMADGAEVMGAI
ARRAGTVYSALTPNMKGFEAALAAGADEAVIFGAASEAFSQRNINCSVAESIARFEPVAAAAKAAGLRLRASISCALGCP
YEGEIAIEKVAEVVSRMRDLGCDEIDIADTIGVGTPLRTQAVMNAAAQVFPRERVAGHFHDTYGQALPNILASLQVGIRI
FHASVAGLGGCPYAKGASGNVATEDLLYMLHGMGIHTGIDLGQVIQAGAFISEAIGRPYGSRVGKALLECSCQQQSA
>Mature_317_residues
MERNAPSLLQALPLPDAVKIVEVGPRDGLQNETHHVPAAVKVELVNRLSEAGFANIEAASFVSPKWVPQMADGAEVMGAI
ARRAGTVYSALTPNMKGFEAALAAGADEAVIFGAASEAFSQRNINCSVAESIARFEPVAAAAKAAGLRLRASISCALGCP
YEGEIAIEKVAEVVSRMRDLGCDEIDIADTIGVGTPLRTQAVMNAAAQVFPRERVAGHFHDTYGQALPNILASLQVGIRI
FHASVAGLGGCPYAKGASGNVATEDLLYMLHGMGIHTGIDLGQVIQAGAFISEAIGRPYGSRVGKALLECSCQQQSA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=293, Percent_Identity=57.3378839590444, Blast_Score=355, Evalue=3e-98,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=54.9488054607508, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=54.9488054607508, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=52.9411764705882, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17510563, Length=291, Percent_Identity=48.1099656357388, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24582381, Length=291, Percent_Identity=56.0137457044674, Blast_Score=347, Evalue=6e-96,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 33036; Mature: 33036

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01062 HMG_COA_LYASE ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNAPSLLQALPLPDAVKIVEVGPRDGLQNETHHVPAAVKVELVNRLSEAGFANIEAAS
CCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
FVSPKWVPQMADGAEVMGAIARRAGTVYSALTPNMKGFEAALAAGADEAVIFGAASEAFS
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCCCEEEEECHHHHHH
QRNINCSVAESIARFEPVAAAAKAAGLRLRASISCALGCPYEGEIAIEKVAEVVSRMRDL
HCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GCDEIDIADTIGVGTPLRTQAVMNAAAQVFPRERVAGHFHDTYGQALPNILASLQVGIRI
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHASVAGLGGCPYAKGASGNVATEDLLYMLHGMGIHTGIDLGQVIQAGAFISEAIGRPYG
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCCHH
SRVGKALLECSCQQQSA
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERNAPSLLQALPLPDAVKIVEVGPRDGLQNETHHVPAAVKVELVNRLSEAGFANIEAAS
CCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
FVSPKWVPQMADGAEVMGAIARRAGTVYSALTPNMKGFEAALAAGADEAVIFGAASEAFS
CCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHCCCCCEEEEECHHHHHH
QRNINCSVAESIARFEPVAAAAKAAGLRLRASISCALGCPYEGEIAIEKVAEVVSRMRDL
HCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHC
GCDEIDIADTIGVGTPLRTQAVMNAAAQVFPRERVAGHFHDTYGQALPNILASLQVGIRI
CCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHASVAGLGGCPYAKGASGNVATEDLLYMLHGMGIHTGIDLGQVIQAGAFISEAIGRPYG
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHCCCHH
SRVGKALLECSCQQQSA
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]