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The map label for this gene is fucA [C]

Identifier: 73541107

GI number: 73541107

Start: 1530086

End: 1530931

Strand: Direct

Name: fucA [C]

Synonym: Reut_A1413

Alternate gene names: 73541107

Gene position: 1530086-1530931 (Clockwise)

Preceding gene: 73541106

Following gene: 73541108

Centisome position: 40.2

GC content: 65.72

Gene sequence:

>846_bases
ATGAACGCCCCGCAAGCCCATCCCGCAGCCGCCGACGAAATCGGCGCCGGCCTGTCCGAAGAGGTCAAGGCCCGCTACCG
CAACCTGCCGCGGCCGCCGGAGTTCGACACGCCCGCGCAGGAACGCCTCCATCGCAAGCAACGCCTTGCCGCGGCGTTCC
GGCTGTTTTCGAAGTTCGGCTTCGATGAAGGCGTCGCCGGCCACATCACGGCGCGCGATCCCGAGCATACCGACACGTTT
TGGGTCAACCCGTTCGGCGTGCATTTCAGCCAGGTGAACGTGTCGAACCTGATCCGCTGCGACCATCACGGCAACGTGGT
GGAAGGCGACTATCCGGTCAACGCAGCAGCCTTTGCAATCCATTCACGCGTGCACCAGACGCGTCCGGACGCGGTCGCCG
CCGCGCACTCGCACAGCACGTATGGCCGCGCATGGTCCACGCTGGGCCGCCCGCTCGACCCGCTCACGCAGGACGTCTGC
GCGTTCTATAACGACCATGCCGTCTATGACGATTTCGGCGGCGTGGTGGTGGAGCTGGACGAGGGCCAGCGCATTGCCAA
TGCGCTGGGATCGAACAAGGCCGCCATCCTGCAGAACCATGGGCTGCTGACGGTCGGTAAGACCGTCGACGAGGCCGCGT
GGTGGTTCATCACGATGGAGCGCTCGTGCCAGGTGCAATTGCTGGCGGAAGCCGCTGCCGCGCGCACCAATGCGCCGCTC
AAGTTCATCTCCGAGGCCGCAGCGAGCCAGGCCTATTCGATCGTGGGTACCGCGCAGGCGGGCTGGTTCCAATTCCAGCC
GCTGTACGCCCGCATCGTCAAAGAGCAGCCCGACCTGCTGGACTGA

Upstream 100 bases:

>100_bases
CAAGCAGCTGGTGAAACTGGTGTAAGCGCCAGTGCGTCGCCGGCCGGGCCTGGCGCCCGGTCGGCGGACCTGCGCACAAC
GACTACCACAGGAGACACGC

Downstream 100 bases:

>100_bases
AGCCATGGCCCCGTTCACCGGCAGGGTGGTACTGATCACGGGTGCGTCGGACGGCATTGGCGCGGAACTCGCGATGCTGC
TGGCACAGCAGGGCGCCCGG

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNAPQAHPAAADEIGAGLSEEVKARYRNLPRPPEFDTPAQERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEHTDTF
WVNPFGVHFSQVNVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRPDAVAAAHSHSTYGRAWSTLGRPLDPLTQDVC
AFYNDHAVYDDFGGVVVELDEGQRIANALGSNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTNAPL
KFISEAAASQAYSIVGTAQAGWFQFQPLYARIVKEQPDLLD

Sequences:

>Translated_281_residues
MNAPQAHPAAADEIGAGLSEEVKARYRNLPRPPEFDTPAQERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEHTDTF
WVNPFGVHFSQVNVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRPDAVAAAHSHSTYGRAWSTLGRPLDPLTQDVC
AFYNDHAVYDDFGGVVVELDEGQRIANALGSNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTNAPL
KFISEAAASQAYSIVGTAQAGWFQFQPLYARIVKEQPDLLD
>Mature_281_residues
MNAPQAHPAAADEIGAGLSEEVKARYRNLPRPPEFDTPAQERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEHTDTF
WVNPFGVHFSQVNVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRPDAVAAAHSHSTYGRAWSTLGRPLDPLTQDVC
AFYNDHAVYDDFGGVVVELDEGQRIANALGSNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTNAPL
KFISEAAASQAYSIVGTAQAGWFQFQPLYARIVKEQPDLLD

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826323, Length=198, Percent_Identity=32.3232323232323, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI29826325, Length=198, Percent_Identity=32.3232323232323, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29826321, Length=198, Percent_Identity=32.3232323232323, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI29826319, Length=198, Percent_Identity=32.3232323232323, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI89276787, Length=198, Percent_Identity=29.7979797979798, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI297307160, Length=198, Percent_Identity=29.7979797979798, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI9257190, Length=198, Percent_Identity=29.7979797979798, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI297307158, Length=198, Percent_Identity=29.7979797979798, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI9257192, Length=198, Percent_Identity=29.7979797979798, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI9943848, Length=198, Percent_Identity=29.2929292929293, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI9951927, Length=198, Percent_Identity=29.2929292929293, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI62912453, Length=198, Percent_Identity=29.2929292929293, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789164, Length=175, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115533216, Length=224, Percent_Identity=26.7857142857143, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533218, Length=224, Percent_Identity=26.7857142857143, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71989541, Length=200, Percent_Identity=28.5, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71989550, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71989560, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71989544, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71989555, Length=200, Percent_Identity=28.5, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281363763, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281363757, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17136738, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281363755, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281363751, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655690, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655685, Length=186, Percent_Identity=34.4086021505376, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363761, Length=186, Percent_Identity=34.4086021505376, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363759, Length=186, Percent_Identity=34.4086021505376, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363753, Length=186, Percent_Identity=34.4086021505376, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 30854; Mature: 30854

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQAHPAAADEIGAGLSEEVKARYRNLPRPPEFDTPAQERLHRKQRLAAAFRLFSKFG
CCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FDEGVAGHITARDPEHTDTFWVNPFGVHFSQVNVSNLIRCDHHGNVVEGDYPVNAAAFAI
CCCCCCCEEECCCCCCCCEEEECCCCCEEEEECHHHHEEECCCCCEECCCCCCCHHHHHH
HSRVHQTRPDAVAAAHSHSTYGRAWSTLGRPLDPLTQDVCAFYNDHAVYDDFGGVVVELD
HHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEEC
EGQRIANALGSNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTNAPL
CCHHHHHHHCCCCEEEEECCCEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCH
KFISEAAASQAYSIVGTAQAGWFQFQPLYARIVKEQPDLLD
HHHHHHHHHHHEEEEEECCCCCEEECHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNAPQAHPAAADEIGAGLSEEVKARYRNLPRPPEFDTPAQERLHRKQRLAAAFRLFSKFG
CCCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
FDEGVAGHITARDPEHTDTFWVNPFGVHFSQVNVSNLIRCDHHGNVVEGDYPVNAAAFAI
CCCCCCCEEECCCCCCCCEEEECCCCCEEEEECHHHHEEECCCCCEECCCCCCCHHHHHH
HSRVHQTRPDAVAAAHSHSTYGRAWSTLGRPLDPLTQDVCAFYNDHAVYDDFGGVVVELD
HHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCEEEEEC
EGQRIANALGSNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTNAPL
CCHHHHHHHCCCCEEEEECCCEEEECCCHHCCEEEEEEECCCCHHHHHHHHHHHCCCCCH
KFISEAAASQAYSIVGTAQAGWFQFQPLYARIVKEQPDLLD
HHHHHHHHHHHEEEEEECCCCCEEECHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]