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The map label for this gene is trmB

Identifier: 73540462

GI number: 73540462

Start: 820572

End: 821342

Strand: Reverse

Name: trmB

Synonym: Reut_A0758

Alternate gene names: 73540462

Gene position: 821342-820572 (Counterclockwise)

Preceding gene: 73540463

Following gene: 73540458

Centisome position: 21.58

GC content: 65.24

Gene sequence:

>771_bases
ATGCACCCCCAAGACGCCAGTACCGAACAGACACCGGTCGACGACGACCAGGTTGAGTCTAGTCAGCCCGTTCATGCTCC
TGAGGACGTCGCCCACCCCCGCCGCATCCGCTCCTTCGTCCGACGCGCCGGCCGTACCTCCAGCGGCCAACAGCGAGCCA
TCGACGAAGTCGGCCCGCGCCTGATAGTGCCTTACGCTCCCCATCCGCTGGACTGGGAAGCCGCCTTTGGCCGCAAGGCC
CCGGCCATCCTCGAGATCGGCTTCGGCATGGGCGAGACGACCGCGCATATCGCGCAACTGCGCCCGCAAGACAATTTCCT
CGGCTGCGAAGTGCACGAGCCTGGCGTCGGCGCGCTGCTCAAGCTGGCCGATGAGCGCGAAATCGGCAATATCCGCATCA
TCCAGCACGATGCGGTGGAAGTCGTCGCTCACATGCTGACCGAGGACTGCCTCGACGGCGTGCATATCTACTTCCCCGAC
CCCTGGCACAAGAAGCGCCACAACAAGCGCCGGCTGGTGCAGCCGCCGCTTGTCAAGCTGCTGGCTTCGCGTCTGAAGCC
GGGCGGCTACATCCATTGCGCGACCGATTGGGAAGAATACGCGCACCAGATGGTGGAAGTGCTGGCGGGCGAGCCTGCAC
TCGAAAATGCGTCGTCGGCCGCCGACGGCTTTTCCGAACGGCCCGAATACCGGCCCATCACCAAGTTCGAACGGCGCGGC
GTGCGGCTCGGGCACGGTGTCTGGGATGTGGTGTTCCGCAAGCGCGGTTGA

Upstream 100 bases:

>100_bases
CATTGGGTTCCTTCGCCGGCTCCGCCTTATATCCGGATAAGCCGCCTGAGCCCCGGTCGCCGGATCAACCGGTGAACCGC
CTCGCGGCTTCTTAGTTGTC

Downstream 100 bases:

>100_bases
TCGGCGCAACCCGACACAAGCAGCCCAAGCAAAAAAAGGCGTGGCACATGGTGGCCACGCCTTTTTTGCATTCAGCCTGA
CGGATCAGGCGTGCCAGGCA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MHPQDASTEQTPVDDDQVESSQPVHAPEDVAHPRRIRSFVRRAGRTSSGQQRAIDEVGPRLIVPYAPHPLDWEAAFGRKA
PAILEIGFGMGETTAHIAQLRPQDNFLGCEVHEPGVGALLKLADEREIGNIRIIQHDAVEVVAHMLTEDCLDGVHIYFPD
PWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLAGEPALENASSAADGFSERPEYRPITKFERRG
VRLGHGVWDVVFRKRG

Sequences:

>Translated_256_residues
MHPQDASTEQTPVDDDQVESSQPVHAPEDVAHPRRIRSFVRRAGRTSSGQQRAIDEVGPRLIVPYAPHPLDWEAAFGRKA
PAILEIGFGMGETTAHIAQLRPQDNFLGCEVHEPGVGALLKLADEREIGNIRIIQHDAVEVVAHMLTEDCLDGVHIYFPD
PWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLAGEPALENASSAADGFSERPEYRPITKFERRG
VRLGHGVWDVVFRKRG
>Mature_256_residues
MHPQDASTEQTPVDDDQVESSQPVHAPEDVAHPRRIRSFVRRAGRTSSGQQRAIDEVGPRLIVPYAPHPLDWEAAFGRKA
PAILEIGFGMGETTAHIAQLRPQDNFLGCEVHEPGVGALLKLADEREIGNIRIIQHDAVEVVAHMLTEDCLDGVHIYFPD
PWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLAGEPALENASSAADGFSERPEYRPITKFERRG
VRLGHGVWDVVFRKRG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=221, Percent_Identity=51.5837104072398, Blast_Score=237, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CUPPJ (Q474P5)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_294982.1
- ProteinModelPortal:   Q474P5
- SMR:   Q474P5
- GeneID:   3609918
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0758
- NMPDR:   fig|264198.3.peg.1272
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   REUT264198:REUT_A0758-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 28734; Mature: 28734

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: BINDING 85-85 BINDING 110-110 BINDING 137-137 BINDING 160-160 BINDING 164-164 BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPQDASTEQTPVDDDQVESSQPVHAPEDVAHPRRIRSFVRRAGRTSSGQQRAIDEVGPR
CCCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCCCE
LIVPYAPHPLDWEAAFGRKAPAILEIGFGMGETTAHIAQLRPQDNFLGCEVHEPGVGALL
EEECCCCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEECEECCCCHHHHH
KLADEREIGNIRIIQHDAVEVVAHMLTEDCLDGVHIYFPDPWHKKRHNKRRLVQPPLVKL
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCCCHHHHH
LASRLKPGGYIHCATDWEEYAHQMVEVLAGEPALENASSAADGFSERPEYRPITKFERRG
HHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCC
VRLGHGVWDVVFRKRG
CCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHPQDASTEQTPVDDDQVESSQPVHAPEDVAHPRRIRSFVRRAGRTSSGQQRAIDEVGPR
CCCCCCCCCCCCCCHHHCCCCCCCCCCHHHCCHHHHHHHHHHHCCCCCCHHHHHHHCCCE
LIVPYAPHPLDWEAAFGRKAPAILEIGFGMGETTAHIAQLRPQDNFLGCEVHEPGVGALL
EEECCCCCCCCCHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCEECEECCCCHHHHH
KLADEREIGNIRIIQHDAVEVVAHMLTEDCLDGVHIYFPDPWHKKRHNKRRLVQPPLVKL
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCCCCCCHHHHH
LASRLKPGGYIHCATDWEEYAHQMVEVLAGEPALENASSAADGFSERPEYRPITKFERRG
HHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCC
VRLGHGVWDVVFRKRG
CCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA