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The map label for this gene is nolF [H]

Identifier: 73540457

GI number: 73540457

Start: 816469

End: 817581

Strand: Reverse

Name: nolF [H]

Synonym: Reut_A0753

Alternate gene names: 73540457

Gene position: 817581-816469 (Counterclockwise)

Preceding gene: 73540458

Following gene: 73540456

Centisome position: 21.48

GC content: 67.48

Gene sequence:

>1113_bases
ATGAAGCGAAACAAGAAGATCCTGATCGCCGTGCTTGGAGCCGTGGCGGTGCTGAGCGCAACGGCCGCCGTGCGCAAGGT
CAGCAGCGCGCGCCAGGCACCCACGCAGACCGCGGCGCCTTCGGTGGTGGAGTTCCTCCAGTCCGACCTGGTACAGGCCG
GTGCACAGGACCTGCGGGTGGCACTGCCGCTGTCGGGCAGCCTGCGCGCACTGAACCAGGCTTCGGTCAAGGCCAAGGTC
TCTGGCGAAGTGCTGCAGGTGCTCGTGCGCGAAGGCGAAGCCGTGCGGGCCGGCCAGGTGATCGTGCGCATCGATCCGAC
CGAATACGAGGCCAAAGTTGCGCAGGCCCGTGGCCAGATGCTGGCGGCACGCGGGCAATACGAGAATTCGCGCCAGACCT
GGGAGCGCAACCGCGAACTGGTCGGCAAGGGCTTTATCTCGAAGACCGCGTTCGACAACTACCAGTCCAACCTGGATGTG
GCCCGCGCCAACCTCGATGCCGCGCAAGGCGGCCTGGCTGTCGCGCAGAAGGCGTTGAACGACACCGTGGTCAAGGCGCC
GCTCGACGGCATGGTGGCCGCGCGCGCTGTGCAGCCTGGCGAGAAGGTATCGCCCGACACGCGCCTGATCGACGTGGTCG
ATTTGCGCGTACTGGAACTGGAAGCCCCGGTGCCGATGGCCGACGTGGCGCGCGCATCGGTGGGCCAGTCCGTACAGCTC
GATGTGGAAGGCGCCGGGCAGTTTGCCGGCGCTCTCGTGCGCATCAACCCGGCCGTGAGCCAGGGGACGCGCAGCATCAT
GGTCTATGTGCGCGTGCAGAACGACGACGGCAAGCTGCGTGCCGGTATGTTCGCGCGGGGCGCGCTGGTGCTAGGCCAGC
GCAGCGGTGTGGTGTCGGTGCCGGCTTCAGCGGTGCGCAGCGACGGCGAACGTGCTTTCGTCTATGTCATCGAGAACGGC
GTCCTCGCCGAGCGCGCGGTGCAGACCGGCGTTCGCGATGAAGGCAGCGGCATGGTGGAAATCGTCTCGGGACTGACAAC
CGGTACCGAGGTCGTGCGCAACAACCTCGGCACGCTGCGCAGCGGCAGCCAGGTGCGCCGCGTGAAGGCCTGA

Upstream 100 bases:

>100_bases
GGGAAAAGATCCGCGATGAAATGACTGCGCAGCGGCTGGGCCAGCGCACCGACGATCTCGACACGGTTGCCCCGGCGGCC
ACCGCACAACAAGACAAGAC

Downstream 100 bases:

>100_bases
GGACGCACCGCCATGTGGTTCACCCGCCTGTCGATCCAGAACCCGGTGCTGGCCACCATGATGATGATGGCGTTCATCGT
CGTGGGCCTGTTCTCGTACC

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKRNKKILIAVLGAVAVLSATAAVRKVSSARQAPTQTAAPSVVEFLQSDLVQAGAQDLRVALPLSGSLRALNQASVKAKV
SGEVLQVLVREGEAVRAGQVIVRIDPTEYEAKVAQARGQMLAARGQYENSRQTWERNRELVGKGFISKTAFDNYQSNLDV
ARANLDAAQGGLAVAQKALNDTVVKAPLDGMVAARAVQPGEKVSPDTRLIDVVDLRVLELEAPVPMADVARASVGQSVQL
DVEGAGQFAGALVRINPAVSQGTRSIMVYVRVQNDDGKLRAGMFARGALVLGQRSGVVSVPASAVRSDGERAFVYVIENG
VLAERAVQTGVRDEGSGMVEIVSGLTTGTEVVRNNLGTLRSGSQVRRVKA

Sequences:

>Translated_370_residues
MKRNKKILIAVLGAVAVLSATAAVRKVSSARQAPTQTAAPSVVEFLQSDLVQAGAQDLRVALPLSGSLRALNQASVKAKV
SGEVLQVLVREGEAVRAGQVIVRIDPTEYEAKVAQARGQMLAARGQYENSRQTWERNRELVGKGFISKTAFDNYQSNLDV
ARANLDAAQGGLAVAQKALNDTVVKAPLDGMVAARAVQPGEKVSPDTRLIDVVDLRVLELEAPVPMADVARASVGQSVQL
DVEGAGQFAGALVRINPAVSQGTRSIMVYVRVQNDDGKLRAGMFARGALVLGQRSGVVSVPASAVRSDGERAFVYVIENG
VLAERAVQTGVRDEGSGMVEIVSGLTTGTEVVRNNLGTLRSGSQVRRVKA
>Mature_370_residues
MKRNKKILIAVLGAVAVLSATAAVRKVSSARQAPTQTAAPSVVEFLQSDLVQAGAQDLRVALPLSGSLRALNQASVKAKV
SGEVLQVLVREGEAVRAGQVIVRIDPTEYEAKVAQARGQMLAARGQYENSRQTWERNRELVGKGFISKTAFDNYQSNLDV
ARANLDAAQGGLAVAQKALNDTVVKAPLDGMVAARAVQPGEKVSPDTRLIDVVDLRVLELEAPVPMADVARASVGQSVQL
DVEGAGQFAGALVRINPAVSQGTRSIMVYVRVQNDDGKLRAGMFARGALVLGQRSGVVSVPASAVRSDGERAFVYVIENG
VLAERAVQTGVRDEGSGMVEIVSGLTTGTEVVRNNLGTLRSGSQVRRVKA

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=317, Percent_Identity=25.5520504731861, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87081796, Length=335, Percent_Identity=28.6567164179104, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=24.4845360824742, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789665, Length=328, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081951, Length=149, Percent_Identity=28.8590604026846, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39027; Mature: 39027

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNKKILIAVLGAVAVLSATAAVRKVSSARQAPTQTAAPSVVEFLQSDLVQAGAQDLRV
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
ALPLSGSLRALNQASVKAKVSGEVLQVLVREGEAVRAGQVIVRIDPTEYEAKVAQARGQM
EECCCCCHHHHHHHHHEEHHHHHHHHHHHHCCCCEEECEEEEEECCCHHHHHHHHHHHHH
LAARGQYENSRQTWERNRELVGKGFISKTAFDNYQSNLDVARANLDAAQGGLAVAQKALN
EECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCHHCCHHHHHHHHHC
DTVVKAPLDGMVAARAVQPGEKVSPDTRLIDVVDLRVLELEAPVPMADVARASVGQSVQL
CCEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCEEEE
DVEGAGQFAGALVRINPAVSQGTRSIMVYVRVQNDDGKLRAGMFARGALVLGQRSGVVSV
EECCCCCCCCEEEEECCHHHCCCEEEEEEEEEECCCCCEEECEEECCEEEEECCCCEEEC
PASAVRSDGERAFVYVIENGVLAERAVQTGVRDEGSGMVEIVSGLTTGTEVVRNNLGTLR
CHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCC
SGSQVRRVKA
CCCHHHHCCC
>Mature Secondary Structure
MKRNKKILIAVLGAVAVLSATAAVRKVSSARQAPTQTAAPSVVEFLQSDLVQAGAQDLRV
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEE
ALPLSGSLRALNQASVKAKVSGEVLQVLVREGEAVRAGQVIVRIDPTEYEAKVAQARGQM
EECCCCCHHHHHHHHHEEHHHHHHHHHHHHCCCCEEECEEEEEECCCHHHHHHHHHHHHH
LAARGQYENSRQTWERNRELVGKGFISKTAFDNYQSNLDVARANLDAAQGGLAVAQKALN
EECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCHHCCHHHHHHHHHC
DTVVKAPLDGMVAARAVQPGEKVSPDTRLIDVVDLRVLELEAPVPMADVARASVGQSVQL
CCEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCEEEE
DVEGAGQFAGALVRINPAVSQGTRSIMVYVRVQNDDGKLRAGMFARGALVLGQRSGVVSV
EECCCCCCCCEEEEECCHHHCCCEEEEEEEEEECCCCCEEECEEECCEEEEECCCCEEEC
PASAVRSDGERAFVYVIENGVLAERAVQTGVRDEGSGMVEIVSGLTTGTEVVRNNLGTLR
CHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCC
SGSQVRRVKA
CCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]