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The map label for this gene is ytwF [H]

Identifier: 73540454

GI number: 73540454

Start: 812133

End: 812456

Strand: Reverse

Name: ytwF [H]

Synonym: Reut_A0750

Alternate gene names: 73540454

Gene position: 812456-812133 (Counterclockwise)

Preceding gene: 73540455

Following gene: 73540453

Centisome position: 21.34

GC content: 67.59

Gene sequence:

>324_bases
ATGCAGGTCATCCAACCCACCGAACTGGCCCAGTGGCTGGCTGATGCCAGCCGCGCCAAGCCGGTCCTGCTGGATGTGCG
TGAGCCGTGGGAGGTGCAGACCTGCGCCATGCCCGGCATCACCCACATCCCCATGCGCGACATCCCGGCGCGCGCCGCCG
AGCTGGACGAGGATGCGGACATCGTCTGCATCTGCCATCACGGCATGCGCAGCATGCAGGTGGCCGCGTTTCTCGAGCGC
CAGGGCTACGCCAAGGTCTACAACCTCACCGGCGGCGTCGACGCCTGGGCGAGCCAGGTAGACCCGGCCATGCCGAAGTA
CTGA

Upstream 100 bases:

>100_bases
CGACACCGAATACCAGGTCGTGAATCTGTTCGAGACCGCGGTCAAGCCGCTGGTCGGCGCGGCCCAGCCGTCGCAATTCC
GGTTCTGAGGAAGCAGCATC

Downstream 100 bases:

>100_bases
GAGGCCGAAGCCCCGCTTGGTTGCCGATCGCCCGCGAAGTCAAACCCAAACAAAAAATGACCGTTGGCCGCGCCTGTGCC
GGCAACCTGGAGATGTGATG

Product: rhodanese-like protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MQVIQPTELAQWLADASRAKPVLLDVREPWEVQTCAMPGITHIPMRDIPARAAELDEDADIVCICHHGMRSMQVAAFLER
QGYAKVYNLTGGVDAWASQVDPAMPKY

Sequences:

>Translated_107_residues
MQVIQPTELAQWLADASRAKPVLLDVREPWEVQTCAMPGITHIPMRDIPARAAELDEDADIVCICHHGMRSMQVAAFLER
QGYAKVYNLTGGVDAWASQVDPAMPKY
>Mature_107_residues
MQVIQPTELAQWLADASRAKPVLLDVREPWEVQTCAMPGITHIPMRDIPARAAELDEDADIVCICHHGMRSMQVAAFLER
QGYAKVYNLTGGVDAWASQVDPAMPKY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11907; Mature: 11907

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVIQPTELAQWLADASRAKPVLLDVREPWEVQTCAMPGITHIPMRDIPARAAELDEDAD
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCC
IVCICHHGMRSMQVAAFLERQGYAKVYNLTGGVDAWASQVDPAMPKY
EEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQVIQPTELAQWLADASRAKPVLLDVREPWEVQTCAMPGITHIPMRDIPARAAELDEDAD
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCC
IVCICHHGMRSMQVAAFLERQGYAKVYNLTGGVDAWASQVDPAMPKY
EEEEECCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]